Estimating age of humans based on telomere shortening.

Abstract:

:To estimate age using DNA based on telomere shortening, we determined the terminal restriction fragment (TRF) length, as telomere length, using Southern blot analysis of peripheral human blood and blood stains. All blood stains had been stored at room temperature for 5 months. The average TRF length clearly showed a tendency to shortening with aging. The formula for age estimation was based on a correlation between average TRF length and age of the subjects. The estimated age calculated from TRF length widely depends on environmental and genetic factors. However, as long as the DNA is well preserved, use of our method is feasible regardless of age of the subject and can give a rough estimation of age of subjects in forensic samples that carry no morphological information.

journal_name

Forensic Sci Int

authors

Tsuji A,Ishiko A,Takasaki T,Ikeda N

doi

10.1016/s0379-0738(02)00086-5

keywords:

subject

Has Abstract

pub_date

2002-05-23 00:00:00

pages

197-9

issue

3

eissn

0379-0738

issn

1872-6283

pii

S0379073802000865

journal_volume

126

pub_type

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