Novel human and mouse genes encoding an acid phosphatase family member and its downregulation in W/W(V) mouse jejunum.


BACKGROUND AND AIMS:Interstitial cells of Cajal (ICC) are pacemakers and mediators of motor neurotransmission in gastrointestinal smooth muscles. ICC require cellular signalling via Kit, a receptor tyrosine kinase, for development and maintenance of phenotype. Much of the evidence demonstrating the functions of ICC comes from studies of W/W(V) mice, which have reduced Kit function and reductions in specific populations of ICC. The aim of the present study was to differentially examine gene expression in the small intestines of wild-type and W/W(V) mutant mice. METHODS AND RESULTS:RNA from the jejunums of wild-type and W/W(V) mutants was analysed using a differential gene display method. Eighteen queries were identified as novel genes that were differentially displayed in wild-type and W/W(V) mice. One candidate gene, encoding a novel acid phosphatase-like protein, was significantly suppressed in fed and starved W/W(V) mice. The full length clone of the murine gene and its human counterpart were designated acid phosphatase-like protein 1 (ACPL1). Human ACPL1 cDNA encodes a protein of 428 amino acids with homology to human prostatic acid phosphatase protein. This gene is located at 1q21. ACPL1 was abundantly expressed in the human small intestine and colon. Gene products were found to be cytoplasmic in transfected COS-7 cells. Reverse transcription-polymerase chain reaction analysis revealed expression of ACPL1 mRNA within single isolated ICCs. CONCLUSIONS:Gene analysis showed that ACPL1 was differentially expressed in the small intestines of normal and W/W(V) mice. ICC within the small intestine expressed mRNA for ACPL1. Specific downregulation of ACPL1 in the jejunums of W/W(V) mice and high expression in human intestinal tissue suggest that the ACPL1 gene could be associated with ICC function in mice and humans.






Takayama I,Daigo Y,Ward SM,Sanders KM,Walker RL,Horowitz B,Yamanaka T,Fujino MA





Has Abstract


2002-06-01 00:00:00














  • Selective deficiency of pancreatic amylase.

    abstract::Two patients with specific pancreatic amylase deficiency are described. The greatly reduced pancreatic amylase activity was not due to an enzymatically inactive amylase molecule but to an almost complete absence of the molecule itself. The findings are of diagnostic importance as they show that low pancreatic amylase ...


    pub_type: 杂志文章


    authors: Sjölund K,Häggmark A,Ihse I,Skude G,Kärnström U,Wikander M

    更新日期:1991-05-01 00:00:00

  • Electrical stimulation and insulin tests used with bilateral selective vagotomy.

    abstract::Completeness of vagotomy has been assessed by use of the electrical stimulation test and the insulin test in 50 patients suffering from chronic duodenal ulceration. A positive result from either was taken as evidence of incomplete vagotomy. The electrical stimulation test provided such evidence more often than the ins...


    pub_type: 杂志文章


    authors: Hollanders D

    更新日期:1971-08-01 00:00:00

  • Diagnostic misclassification reduces the ability to detect linkage in inflammatory bowel disease genetic studies.

    abstract:BACKGROUND:Linkage data have now identified several inflammatory bowel disease (IBD) susceptibility loci but these data have not been consistently replicated in independent studies. One potential explanation for this is the possibility that patients enrolled in such studies may have been erroneously classified with res...


    pub_type: 杂志文章


    authors: Silverberg MS,Daly MJ,Moskovitz DN,Rioux JD,McLeod RS,Cohen Z,Greenberg GR,Hudson TJ,Siminovitch KA,Steinhart AH

    更新日期:2001-12-01 00:00:00

  • Endothelial progenitors promote hepatocarcinoma intrahepatic metastasis through monocyte chemotactic protein-1 induction of microRNA-21.

    abstract:OBJECTIVES:Endothelial progenitor cells (EPCs) circulate with increased numbers in the peripheral blood of patients with highly-vascularised hepatocellular carcinoma (HCC) and contribute to angiogenesis and neovascularisation. We hypothesised that angiogenic EPCs, that is, colony forming unit-endothelial cells (CFU-ECs...


    pub_type: 杂志文章


    authors: Shih YT,Wang MC,Zhou J,Peng HH,Lee DY,Chiu JJ

    更新日期:2015-07-01 00:00:00

  • Diagnosis of small bowel Crohn's disease: a prospective comparison of capsule endoscopy with magnetic resonance imaging and fluoroscopic enteroclysis.

    abstract:BACKGROUND AND AIMS:The diagnostic yield of capsule endoscopy (CE) compared with magnetic resonance imaging (MRI) in small bowel Crohn's disease is not well established. We prospectively investigated CE, MRI, and double contrast fluoroscopy in patients with suspected small bowel Crohn's disease. METHODS:Fifty two cons...


    pub_type: 杂志文章


    authors: Albert JG,Martiny F,Krummenerl A,Stock K,Lesske J,Göbel CM,Lotterer E,Nietsch HH,Behrmann C,Fleig WE

    更新日期:2005-12-01 00:00:00

  • Inflammatory bowel disease and risk of small bowel cancer: a binational population-based cohort study from Denmark and Sweden.

    abstract:OBJECTIVE:Crohn's disease (CD) is associated with increased risk of small bowel cancer (SBC), but previous studies have been small. We aimed to examine the risk of incident SBC and death from SBC in patients with inflammatory bowel disease (IBD). DESIGN:In a binational, population-based cohort study from Sweden and De...


    pub_type: 杂志文章


    authors: Axelrad JE,Olén O,Sachs MC,Erichsen R,Pedersen L,Halfvarson J,Askling J,Ekbom A,Sørensen HT,Ludvigsson JF

    更新日期:2021-02-01 00:00:00

  • Narrow band imaging for colonoscopic surveillance in hereditary non-polyposis colorectal cancer.

    abstract:BACKGROUND:Colonoscopic surveillance for hereditary non-polyposis colorectal cancer (HNPCC) reduces death rates, but early interval cancers still occur, probably due to missed small, aggressive adenomas. Narrow band imaging (NBI), a novel endoscopic technology, highlights superficial mucosal capillaries and improves co...


    pub_type: 杂志文章


    authors: East JE,Suzuki N,Stavrinidis M,Guenther T,Thomas HJ,Saunders BP

    更新日期:2008-01-01 00:00:00

  • Evolution of hereditary non-polyposis colorectal cancer.

    abstract::The prevalence of colorectal adenoma in 23 affected patients with hereditary non-polyposis colorectal cancer (HNPCC) was compared with that in an age matched forensic autopsy population. The mean ages at diagnosis of colorectal cancer in men (13) and women (10) were 39 and 37 years respectively. In HNPCC patients who ...


    pub_type: 杂志文章


    authors: Jass JR,Stewart SM

    更新日期:1992-06-01 00:00:00

  • Hedgehog inhibition prolongs survival in a genetically engineered mouse model of pancreatic cancer.

    abstract:BACKGROUND AND AIMS:Pancreatic cancer is among the most dismal of human malignancies. Current therapeutic strategies are virtually ineffective in controlling advanced, metastatic disease. Recent evidence suggests that the Hedgehog signalling pathway is aberrantly reactivated in the majority of pancreatic cancers, and t...


    pub_type: 杂志文章


    authors: Feldmann G,Habbe N,Dhara S,Bisht S,Alvarez H,Fendrich V,Beaty R,Mullendore M,Karikari C,Bardeesy N,Ouellette MM,Yu W,Maitra A

    更新日期:2008-10-01 00:00:00

  • Development of colonic neoplasia in p53 deficient mice with experimental colitis induced by dextran sulphate sodium.

    abstract:BACKGROUND:Several animal models for human ulcerative colitis (UC) associated neoplasia have been reported. However, most neoplasias developed in these models have morphological and genetic characteristics different from UC associated neoplasia. AIMS:To establish a new colitis associated neoplasia model in p53 deficie...


    pub_type: 杂志文章


    authors: Fujii S,Fujimori T,Kawamata H,Takeda J,Kitajima K,Omotehara F,Kaihara T,Kusaka T,Ichikawa K,Ohkura Y,Ono Y,Imura J,Yamaoka S,Sakamoto C,Ueda Y,Chiba T

    更新日期:2004-05-01 00:00:00

  • Nerve activity recordings in routine human intestinal biopsies.

    abstract:BACKGROUND:Most direct understanding of enteric nerve (patho)physiology has been obtained by electrode and imaging techniques in animal models and human surgical samples. Until now, neuronal activity recordings from a more accessible human tissue source have remained a true challenge. OBJECTIVES:To record nerve activi...


    pub_type: 杂志文章


    authors: Cirillo C,Tack J,Vanden Berghe P

    更新日期:2013-02-01 00:00:00

  • Chronic intrahepatic cholestasis due to sarcoidosis.

    abstract::Two West Indian patients with Kveim-biopsy proven sarcoidosis developed chronic cholestatic liver disease, clinically and biochemically similar to primary biliary cirrhosis. Liver histology revealed multiple granulomas with reduction in bile ducts and, in one patient, progression to biliary cirrhosis. Portal hypertens...


    pub_type: 杂志文章


    authors: Bass NM,Burroughs AK,Scheuer PJ,James DG,Sherlock S

    更新日期:1982-05-01 00:00:00

  • Myristoleic acid produced by enterococci reduces obesity through brown adipose tissue activation.

    abstract:OBJECTIVE:Dietary fibre has beneficial effects on energy metabolism, and the majority of studies have focused on short-chain fatty acids produced by gut microbiota. Ginseng has been reported to aid in body weight management, however, its mechanism of action is not yet clear. In this study, we focused on the potential m...


    pub_type: 杂志文章


    authors: Quan LH,Zhang C,Dong M,Jiang J,Xu H,Yan C,Liu X,Zhou H,Zhang H,Chen L,Zhong FL,Luo ZB,Lam SM,Shui G,Li D,Jin W

    更新日期:2020-07-01 00:00:00

  • Normal deglutitive responses of the human lower oesophageal sphincter.

    abstract::The responses to deglutition of the lower oesophageal high pressure zone were studied in 13 healthy subjects. The recording assembly included three perfused pressure recording catheters, whose recording orifices were at the same axial level, and a more distally situated pH electrode. Relaxation and contraction progres...


    pub_type: 杂志文章


    authors: Kaye MD,Showalter JP

    更新日期:1972-05-01 00:00:00

  • Value of exfoliative cytology for investigating bile duct strictures.

    abstract::The cause of a biliary tract stricture may be difficult to determine radiologically. Exfoliative biliary cytology was evaluated in 62 patients (median age 65 years, range 30-94) with biliary tract strictures presenting to the Hepatobiliary Unit between January 1984 and December 1989. Bile samples were taken during end...


    pub_type: 杂志文章


    authors: Davidson B,Varsamidakis N,Dooley J,Deery A,Dick R,Kurzawinski T,Hobbs K

    更新日期:1992-10-01 00:00:00

  • Computer aided positioning of manometric probe in the upper digestive tract.

    abstract::A method not needing fluoroscopic control for positioning a manometric probe in the gastroduodenal tract is described. It is based on hydrostatic pressure variations occurring at each recording port of a multichannel perfused probe after changes in patient's position. The data are fed into a computer and the schematic...


    pub_type: 杂志文章


    authors: Bortolotti M,Bersani G

    更新日期:1988-07-01 00:00:00

  • Intestinal cells produce basement membrane proteins in vitro.

    abstract::The epithelial-mesenchymal interface of the intestinal mucosa obviously plays an important role in supporting the mucosal architecture. Its significance for the process of migration and differentiation of the epithelial cells remains to be resolved. It consists of a basement membrane, the anchoring zone and the subepi...


    pub_type: 杂志文章


    authors: Hahn U,Schuppan D,Hahn EG,Merker HJ,Riecken EO

    更新日期:1987-01-01 00:00:00

  • Immune activation by DNA damage predicts response to chemotherapy and survival in oesophageal adenocarcinoma.

    abstract:OBJECTIVE:Current strategies to guide selection of neoadjuvant therapy in oesophageal adenocarcinoma (OAC) are inadequate. We assessed the ability of a DNA damage immune response (DDIR) assay to predict response following neoadjuvant chemotherapy in OAC. DESIGN:Transcriptional profiling of 273 formalin-fixed paraffin-...


    pub_type: 杂志文章


    authors: Turkington RC,Knight LA,Blayney JK,Secrier M,Douglas R,Parkes EE,Sutton EK,Stevenson L,McManus D,Halliday S,McCavigan AM,Logan GE,Walker SM,Steele CJ,Perner J,Bornschein J,MacRae S,Miremadi A,McCarron E,McQuaid S,

    更新日期:2019-11-01 00:00:00

  • High frequency of helicobacter negative gastritis in patients with Crohn's disease.

    abstract::The frequency of gastric Crohn's disease has been considered low. This study was undertaken to determine the prevalence of chronic gastritis and Helicobacter pylori infection in patients with Crohn's disease. Oesophagogastroduodenoscopy was performed on 62 consecutive patients suffering from ileocolonic Crohn's diseas...


    pub_type: 杂志文章


    authors: Halme L,Kärkkäinen P,Rautelin H,Kosunen TU,Sipponen P

    更新日期:1996-03-01 00:00:00

  • The cardiac sphincter in the cat.

    abstract::A study of the function of the lower end of the oesophagus in the cat confirms that it behaves as a true physiological sphincter, even though histological evidence for such a structure is absent. Sphincteric action is a function of the circular muscle fibres in the lowest 2 cm. of the oesophagus. Reflex changes in ton...


    pub_type: 杂志文章


    authors: CLARK CG,VANE JR

    更新日期:1961-09-01 00:00:00

  • Qualitative and quantitative evaluation of internal anal sphincter function in the newborn.

    abstract::One-hundred-and-one normal neonates were examined manometrically in the first day of life to assess anorectal function. In order to overcome some of the difficulties in subjective interpretation of results, the stimuli used and the response pattern seen in the anal canal were measured. In the unstimulated state, all s...


    pub_type: 杂志文章


    authors: Boston VE,Cywes S,Davies MR

    更新日期:1977-12-01 00:00:00

  • Serum amylase and serum lipase levels in man after administration of secretin and pancreozymin.

    abstract::A simple evocative test has been used to study pancreatic function. Serial estimations of amylase and lipase in blood serum are made at intervals up to six hours and again at 24 hours after injecting intravenously standard doses of secretin and pancreozymin. The results of 213 tests on a normal group, in pancreatic di...


    pub_type: 杂志文章



    更新日期:1960-06-01 00:00:00

  • Effect of ursodeoxycholic acid on methionine adenosyltransferase activity and hepatic glutathione metabolism in rats.

    abstract:BACKGROUND AND AIMS:Both bile salts and glutathione participate in the generation of canalicular bile flow. In this work, we have investigated the effect of different bile salts on hepatic glutathione metabolism. METHODS:Using the isolated and perfused rat liver, we studied hepatic glutathione content, and metabolism ...


    pub_type: 杂志文章


    authors: Rodríguez-Ortigosa CM,Cincu RN,Sanz S,Ruiz F,Quiroga J,Prieto J

    更新日期:2002-05-01 00:00:00

  • JC virus infects the enteric glia of patients with chronic idiopathic intestinal pseudo-obstruction.

    abstract:BACKGROUND AND AIMS:Chronic idiopathic intestinal pseudo-obstruction (CIIP) is characterised by severe impairment of intestinal propulsive motility that mimics bowel obstruction. JC virus (JCV) is a polyomavirus that can infect brain glial cells causing a fatal disease, but may also be found throughout the normal gastr...


    pub_type: 杂志文章


    authors: Selgrad M,De Giorgio R,Fini L,Cogliandro RF,Williams S,Stanghellini V,Barbara G,Tonini M,Corinaldesi R,Genta RM,Domiati-Saad R,Meyer R,Goel A,Boland CR,Ricciardiello L

    更新日期:2009-01-01 00:00:00

  • Increased risk and case fatality rate of pyogenic liver abscess in patients with liver cirrhosis: a nationwide study in Denmark.

    abstract:BACKGROUND:Patients with liver cirrhosis are at increased risk of serious bacterial infections carrying a high case fatality rate. Case reports have suggested an association between liver cirrhosis and pyogenic liver abscess. AIMS:To estimate the risk and case fatality rate of pyogenic liver abscess in Danish patients...


    pub_type: 杂志文章


    authors: Mølle I,Thulstrup AM,Vilstrup H,Sørensen HT

    更新日期:2001-02-01 00:00:00

  • Prostaglandin E2 is a mediator of 5-hydroxytryptamine induced water and electrolyte secretion in the human jejunum.

    abstract::Studies in the rat jejunum in vivo have shown that 5-hydroxytryptamine (5-HT) causes secretion of fluid and luminal release of prostaglandin (PG) E2. These effects can be blocked by indomethacin and ketanserin, which suggests that PGE2 may be an important intermediate in the transduction mechanism leading to 5-HT indu...


    pub_type: 杂志文章


    authors: Munck LK,Mertz-Nielsen A,Westh H,Bukhave K,Beubler E,Rask-Madsen J

    更新日期:1988-10-01 00:00:00

  • Behaviour of acid secretion, gastrin release, serum pepsinogen I, and gastric emptying of liquids over six months from eradication of helicobacter pylori in duodenal ulcer patients. A controlled study.

    abstract::The behaviour of basal and stimulated acid secretion, gastrin release, serum pepsinogen I, and gastric emptying of liquids was studied in 19 consecutive patients with Helicobacter pylori positive duodenal ulcer, over a follow up period of six months. Eleven patients were studied before and at three and six months afte...


    pub_type: 杂志文章


    authors: Parente F,Maconi G,Sangaletti O,Minguzzi M,Vago L,Bianchi Porro G

    更新日期:1995-08-01 00:00:00

  • Goseki histological grading of gastric cancer is an important predictor of outcome.

    abstract::TNM (tumour, node, metastases) staging has thus far been the most important guide to prognosis in patients with gastric cancer. Histological grading, in contrast, has not provided any additional information. Recently a novel grading system based on tubular differentiation and mucus production has been proposed, which ...


    pub_type: 杂志文章


    authors: Martin IG,Dixon MF,Sue-Ling H,Axon AT,Johnston D

    更新日期:1994-06-01 00:00:00

  • Predictive value of oesophageal pH variables in children with gastro-oesophageal reflux.

    abstract::Sixty three 24 hour oesophageal pH studies were performed in children with known or suspected gastro-oesophageal reflux and their progress was followed for at least a year. Forty two studies were of children who were well at follow up who were not receiving medical treatment for gastro-oesophageal reflux, and had not ...


    pub_type: 杂志文章


    authors: Colson DJ,Campbell CA,Wright VA,Watson BW

    更新日期:1990-04-01 00:00:00

  • The British Society of Gastroenterology early gastric cancer/dysplasia survey: an interim report.

    abstract::This presentation describes interim findings in a series of 319 patients referred from 41 hospitals on the basis of histopathological findings of 'early gastric cancer', 'dysplasia', or 'worrying mucosal appearances'. Data were recorded using a predefined proforma, and histopathological material circulated amongst a '...


    pub_type: 临床试验,杂志文章,多中心研究


    authors: de Dombal FT,Price AB,Thompson H,Williams GT,Morgan AG,Softley A,Clamp SE,Unwin BJ

    更新日期:1990-01-01 00:00:00