Abstract：
:Immobilization of biomolecules on surfaces enables both localization and retention of molecules at the cell-biomaterial interface. Since metallic biomaterials used for orthopedic and dental implants possess a paucity of reactive functional groups, biomolecular modification of these materials is challenging. In the present work, we investigated the use of a plasma surface modification strategy to enable immobilization of bioactive molecules on a "bioinert" metal. Conditions during plasma polymerization of allyl amine on Ti-6Al-4V were varied to yield 5 ("low")- and 12 ("high")-NH2/nm2. One- and two-step carbodiimide schemes were used to immobilize lysozyme, a model biomolecule, and bone morphogenetic protein-4 (BMP-4) on the aminated surfaces. Both schemes could be varied to control the amount of protein bound, but the one-step method destroyed the activity of immobilized lysozyme because of crosslinking. BMP-4 was then immobilized using the two-step scheme. Although BMP bound to both low- and high-NH2 surfaces was initially able to induce alkaline phosphatase activity in pluripotent C3H10T1/2 cells, only high amino group surfaces were effective following removal of weakly bound protein by incubation in cell culture medium.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Puleo DA,Kissling RA,Sheu MSdoi
10.1016/s0142-9612(01)00339-8keywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
2079-87issue
9eissn
0142-9612issn
1878-5905pii
S0142-9612(01)00339-8journal_volume
23pub_type
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