Abstract：
:We have developed nanoparticles based on Murine Leukemia Virus virus-like-particles (VLPs) that efficiently deliver therapeutic bioactive proteins in their native state into target cells. Nuclear transcription factors and toxic proteins were incorporated into the VLPs from stable producer cells without transducing viral-encoded genetic material. Delivery of nuclear transcription factors required incorporation of nuclear export signals (NESs) into the vector backbone for the efficient formation of VLPs. In the presence of an appropriate targeting Env glycoprotein, transcription factors delivered and activated nuclear transcription in the target cells. Additionally, we show delivery of the bacterial toxin, MazF, which is an ACA-specific mRNA interferase resulted in the induction of cell death. The stable producer cells are protected from the toxin through co-expression of the anti-toxin MazE and continuously released MazF incorporating VLPs. This highly adaptable platform can be harnessed to alter and regulate cellular processes by bioactive protein delivery.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Wu DT,Roth MJdoi
10.1016/j.biomaterials.2014.06.006subject
Has Abstractpub_date
2014-09-01 00:00:00pages
8416-26issue
29eissn
0142-9612issn
1878-5905pii
S0142-9612(14)00682-6journal_volume
35pub_type
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