Lipopolysaccharide upregulates the expression of Toll-like receptor 4 in human vascular endothelial cells.

Abstract:

OBJECTIVE:To investigate the expression of Toll-like receptor 2 (TLR2) and Toll-like receptor 4 (TLR4) and the effect of lipopolysaccharide (LPS) on their expression in cultured endothelial cells. METHODS:Total RNA was extracted from ECV304 cells and isolated human umbilical vein endothelial cells (HUVECs) exposed to LPS, respectively. The quantification of TLR2 and TLR4 mRNA in HUVECs and EVC304 cells was carried out by reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS:ECV304 cells and HUVECs were able to express TLR2 and TLR4 mRNA, but the expression levels of TLR4 appeared to be stronger than those of TLR2. LPS could upregulate the expression levels of TLR4 obviously, whereas it had no effect on the expression level of TLR2. CONCLUSIONS:Our data indicate that TLR4 may be the LPS signal transducer in endothelial cells and plays important roles in the cell activation of LPS. The ECV304 cell line is a better experimental model than isolated HUVECs in the research of endothelial cells.

journal_name

Chin Med J (Engl)

journal_title

Chinese medical journal

authors

Yang Q,Zhu P,Wang Z,Jiang J

keywords:

subject

Has Abstract

pub_date

2002-02-01 00:00:00

pages

286-9

issue

2

eissn

0366-6999

issn

2542-5641

journal_volume

115

pub_type

杂志文章
  • Endodontic bacteria from primary and persistent endodontic lesions in Chinese patients as identified by cloning and 16S ribosomal DNA gene sequencing.

    abstract:BACKGROUND:Few literatures pertain to the 16S ribosomal DNA (16S rDNA) analysis of bacteria contributing to primary and persistent endodontic lesions, with no information available for the Chinese population. As such, we investigated endodontic bacteria associated with primary and persistent endodontic lesions in adult...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Li X,Zhu XF,Zhang CF,Cathro P,Seneviratne CJ,Shen S

    更新日期:2013-02-01 00:00:00

  • A Dosimetric Comparison between Conventional Fractionated and Hypofractionated Image-guided Radiation Therapies for Localized Prostate Cancer.

    abstract:BACKGROUND:Image-guided radiation therapy (IGRT) is the preferred method for curative treatment of localized prostate cancer, which could improve disease outcome and reduce normal tissue toxicity reaction. IGRT using cone-beam computed tomography (CBCT) in combination with volumetric-modulated arc therapy (VMAT) potent...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.4103/0366-6999.183429

    authors: Li M,Li GF,Hou XY,Gao H,Xu YG,Zhao T

    更新日期:2016-06-20 00:00:00

  • A study of medial and lateral temporal lobe epilepsy based on stereoelectroencephalography.

    abstract:BACKGROUND:Patients with temporal lobe epilepsy (TLE) originating from different seizure onset zones had distinct electrophysiological characteristics and surgical outcomes. In this study, we aimed to investigate the relationship between the origin and prognosis of TLE, and the stereoelectroencephalography (SEEG) featu...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.1097/CM9.0000000000001256

    authors: Yan XM,Xu CP,Wang YP,Ma K,Yu T,Zhang XH,Zhang X,Gao RS,Zhang GJ,Li YJ

    更新日期:2020-12-02 00:00:00

  • Analyses of Genotypes and Phenotypes of Ten Chinese Patients with Wolf-Hirschhorn Syndrome by Multiplex Ligation-dependent Probe Amplification and Array Comparative Genomic Hybridization.

    abstract:BACKGROUND:Wolf-Hirschhorn syndrome (WHS) is a contiguous gene syndrome that is typically caused by a deletion of the distal portion of the short arm of chromosome 4. However, there are few reports about the features of Chinese WHS patients. This study aimed to characterize the clinical and molecular cytogenetic featur...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.4103/0366-6999.177996

    authors: Yang WX,Pan H,Li L,Wu HR,Wang ST,Bao XH,Jiang YW,Qi Y

    更新日期:2016-03-20 00:00:00

  • Diagnosis and treatment of solid brainstem tumors in adults.

    abstract:OBJECTIVE:To evaluate the diagnosis and aggressive surgical approach for brainstem tumors. METHODS:A series of 24 adult patients with brainstem tumors were involved. Magnetic resonance (MR) imaging was performed on all patients. Surgical technique, potential risk and management of complications were described. RESULT...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Xu Q,Bao W,Mao R

    更新日期:1997-02-01 00:00:00

  • Changes of serial cerebrospinal fluid immunoglobulin parameters in neurological disorders.

    abstract::Serial cerebrospinal fluid immunoglobulin parameters were determined with the method of single radial immunodiffusion in 419 cases of 20 kinds of neurological diseases. The results were compared with the normal values of 36 subjects of corresponding ages and statistically analyzed. The authors believe that the elevate...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Wang WZ,Zhou YK

    更新日期:1989-03-01 00:00:00

  • Noninvasive three-dimensional computed tomographic angiography in preoperative detection of intracranial arteriovenous malformations.

    abstract:OBJECTIVE:To assess the value of noninvasive three-dimensional computed tomographic angiography (3D-CTA) in preoperative detection of intracranial arteriovenous malformations (AVMs). METHODS:A prospective evaluation at a single institute over a 2-year period included 23 patients suspected of intracranial AVMs. All pat...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Wu J,Chen X,Shi Y,Chen S

    更新日期:2000-10-01 00:00:00

  • Molecular epidemiology of serotype 19A Streptococcus pneumoniae isolated from children in Beijing, 1997-2006.

    abstract:BACKGROUND:Despite the prevalence of Streptococcus pneumoniae serotype 19A, the molecular characteristics of this serotype are yet to be fully elucidated. The aim of this study was therefore to determine the homology of the serotype 19A in China. METHODS:Pulsed-field gel electrophoresis and multilocus sequence typing ...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Xue L,Yao KH,Yu SJ,Liu ZJ,Qian J,Shen XZ,Yang YH

    更新日期:2011-06-01 00:00:00

  • Specialized Pro-resolving Mediators Regulate Alveolar Fluid Clearance during Acute Respiratory Distress Syndrome.

    abstract:Objective:Acute respiratory distress syndrome (ARDS) is an acute and lethal clinical syndrome that is characterized by the injury of alveolar epithelium, which impairs active fluid transport in the lung, and impedes the reabsorption of edema fluid from the alveolar space. This review aimed to discuss the role of pro-re...

    journal_title:Chinese medical journal

    pub_type: 杂志文章,评审

    doi:10.4103/0366-6999.229890

    authors: Wang Q,Yan SF,Hao Y,Jin SW

    更新日期:2018-04-20 00:00:00

  • Generation and analysis of 113 adult stage Schistosoma japonicum (Chinese strain) expressed sequence tags.

    abstract:OBJECTIVE:To rapidly and economically obtain knowledge about adult stage Schistosoma japonicum (Chinese strain) expressed genes using expressed sequence tag (EST). METHODS:A directional cDNA library constructed from Schistosoma japonicum (Chinese strain) adult stage RNA was used to generate expressed sequence tags (ES...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Bian G,Yu X,Wu Z

    更新日期:2002-10-01 00:00:00

  • Long-term immunogenicity and efficacy of recombinant yeast derived hepatitis B vaccine for interruption of mother-infant transmission of hepatitis B virus.

    abstract::Recombinant DNA Yeast-Derived Hepatitis B Vaccine (RYHB vaccine) is comparable to and can replace Plasma-Derived Hepatitis B Vaccine (PHB vaccine) for the prevention of mother-infant transmission of hepatitis B virus (HBV), but the duration of immune efficacy of RYHB vaccine is not clear. This study indicates the long...

    journal_title:Chinese medical journal

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Zhu QR,Gu XH,Duan SC,Xu HF

    更新日期:1994-12-01 00:00:00

  • The more, the less: age and chemotherapy load are predictive of poor stem cell mobilization in patients with hematologic malignancies.

    abstract:BACKGROUND:Intensive treatment such as autologous peripheral blood stem cell (PBSC) transplantation is an important therapeutic strategy in many hematologic malignancies. A number of factors have been reported to impact PBSC mobilization, but the predictive factors varied from one study to another. This retrospective s...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Yang SM,Chen H,Chen YH,Zhu HH,Zhao T,Liu KY

    更新日期:2012-02-01 00:00:00

  • The role of CTLA4-Ig in a mouse model against allergic asthma.

    abstract:OBJECTIVE:To investigate CTLA4-Ig's potential role in therapy for allergic asthma by blocking B7/CD28 interactions with cytotoxic T lymphocyte antigen 4-Ig (CTLA4-Ig). METHODS:We divided BALB/C mice into the three groups: Sham/Sham (control), ovalbumin (OVA)/OVA and mCTLA4-Ig. Blood, bronchoalveolar lavage, histology ...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Wan H,Zhou M,Xu Q,Huang S,Deng W

    更新日期:2003-03-01 00:00:00

  • Iron chelator daphnetin against Pneumocystis carinii in vitro.

    abstract:BACKGROUND:Although there are several drugs and drug combinations for the treatment of Pneumocystis carinii (P. carinii) pneumonia, all drugs have the toxicity as well as low efficacy. Iron chelators have been proposed as a source of new drugs for combating these infections. We hypothesized that iron chelators would su...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Ye B,Zheng YQ,Wu WH,Zhang J

    更新日期:2004-11-01 00:00:00

  • Radiation hormesis in cancer mortality.

    abstract::This review of recent literature presents new concepts to evaluate radiation hormesis in cancer mortality. Using these interpretations cancer mortality rates were found to be significantly reduced following whole body exposure to low doses of either acute or chronic irradiation. The threshold dose, the zero equivalent...

    journal_title:Chinese medical journal

    pub_type: 杂志文章,评审

    doi:

    authors: Lukey TD

    更新日期:1994-08-01 00:00:00

  • Induction and deduction in sepsis-induced cardiomyopathy: five typical categories.

    abstract::Sepsis is defined as life-threatening organ dysfunction caused by a dysregulated host response to infection. The heart is one of the most important oxygen delivery organs, and dysfunction significantly increases the mortality of the body. Hence, the heart has been studied in sepsis for over half a century. However, th...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.1097/CM9.0000000000000929

    authors: Wang J,Wang XT,Liu DW,Zhang HM,Su LX

    更新日期:2020-09-20 00:00:00

  • Age-specific differences in non-cardiac comorbidities among elderly patients hospitalized with heart failure: a special focus on young-old, old-old, and oldest-old.

    abstract:BACKGROUND:Despite the growing epidemic of heart failure (HF), there is limited data available to systematically compare non-cardiac comorbidities in the young-old, old-old, and oldest-old patients hospitalized for HF. The precise differences will add valuable information for better management of HF in elderly patients...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.1097/CM9.0000000000000560

    authors: Yang MX,An H,Fan XQ,Tao LY,Tu Q,Qin L,Zhang LF,Feng DP,Wang Y,Sun L,Gao S,Guan WZ,Zheng JG,Ren JY

    更新日期:2019-12-20 00:00:00

  • Incidence and Risk Factors of De novo Stress Urinary Incontinence after Pelvic Floor Reconstruction: A Nested Case-control Study.

    abstract:BACKGROUND:Some patients with pelvic organ prolapse may suffer from lower urinary tract symptoms (LUTS), especially stress urinary incontinence (SUI) named de novo SUI after pelvic floor reconstruction. This study aimed to investigate the incidence and risk factors of de novo SUI. METHODS:This is a nested case-control...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.4103/0366-6999.201592

    authors: Wang SY,Cao TT,Wang RZ,Yang X,Sun XL,Wang JL

    更新日期:2017-03-20 00:00:00

  • Bone marrow-derived mesenchymal stem cells protect rats from endotoxin-induced acute lung injury.

    abstract:BACKGROUND:Acute lung injury (ALI) is a serious and common condition for which there are currently no specific strategies for treatment. Recent studies have suggested that bone marrow-derived multipotent mesenchymal stem cells (MSCs) may have therapeutic applications in multiple clinical disorders. We explored the biol...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Liang ZX,Sun JP,Wang P,Tian Q,Yang Z,Chen LA

    更新日期:2011-09-01 00:00:00

  • Renal metastases of choriocarcinoma. A clinicopathological study of 31 cases.

    abstract::From 1949 through 1978, 31 patients with renal metastasis were diagnosed in a total of 448 cases of choriocarcinoma admitted to our hospital, giving an incidence of 6.9%. Renal metastasis was invariably preceded by pulmonary metastases and usually accompanied by other visceral metastases, indicating that renal metasta...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Wang YE,Song HZ,Yang XY,Dong SY,Gan N

    更新日期:1991-09-01 00:00:00

  • Cutaneous Rosai-Dorfman disease presenting as a granulomatous rosacea-like rashs.

    abstract::A case of cutaneous Rosai-Dorfman disease (CRDD) presenting as a granulomatous rosacea-like rashs was reported. A 45-year-old Chinese woman presented with a 1-month history of a widespread nonpruiginous papulonodular eruption. The rash had begun on her face and rapidly progressed to involve the neck and extremities. S...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Shi XY,Ma DL,Fang K

    更新日期:2011-03-01 00:00:00

  • Menorrhagia and Uterine Volume Associated with Lower Urinary Tract Symptoms in Patients with Adenomyosis.

    abstract:BACKGROUND:Adenomyosis is a gynecological disorder with symptoms most presenting as dysmenorrhea and heavy menstrual bleeding. However, the presence of lower urinary tract symptoms (LUTS) among women with adenomyosis remains unclear. This study was designed to determine the prevalence of LUTS and factors related to the...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.4103/0366-6999.208232

    authors: Li T,Xu XX,Dai Y,Zhang JJ,Lang JH,Leng JH

    更新日期:2017-07-05 00:00:00

  • Fetal Hemodynamics and Fetal Growth Indices by Ultrasound in Late Pregnancy and Birth Weight in Gestational Diabetes Mellitus.

    abstract:BACKGROUND:The offspring of women with gestational diabetes mellitus (GDM) are prone to macrosomia. However, birth weight is difficult to be correctly estimated by ultrasound because of fetal asymmetric growth characteristics. This study aimed to investigate the correlations between fetal hemodynamics, fetal growth ind...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.4103/0366-6999.189057

    authors: Liu F,Liu Y,Lai YP,Gu XN,Liu DM,Yang M

    更新日期:2016-09-05 00:00:00

  • Neutrophil to lymphocyte ratio is a prognosis factor for post-operative pneumonia in aneurysmal subarachnoid hemorrhage patients.

    abstract:BACKGROUND:Although a variety of risk factors of pneumonia after clipping or coiling of the aneurysm (post-operative pneumonia [POP]) in patients with aneurysmal subarachnoid hemorrhage (aSAH) have been studied, the predictive model of POP after aSAH has still not been well established. Thus, the aim of this study was ...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.1097/CM9.0000000000001304

    authors: Chen Y,Lian BQ,Peng L,Ding CY,Lin YX,Yu LH,Wang DL,Kang DZ

    更新日期:2020-12-24 00:00:00

  • Effect of gingerol on substance P and NK1 receptor expression in a vomiting model of mink.

    abstract:BACKGROUND:Gingerol is the generic term for pungent constituents in ginger, which has been reported to be effective for inhibiting vomiting. We attempted to investigate the antiemetic effect of gingerol and its effective mechanism on substance P and NK(1) receptors in minks. METHODS:The antiemetic effect of gingerol w...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Qian QH,Yue W,Chen WH,Yang ZH,Liu ZT,Wang YX

    更新日期:2010-02-20 00:00:00

  • Efficacy and safety investigation of Kuntai capsule for the add-back therapy of gonadotropin releasing hormone agonist administration to endometriosis patients: a randomized, double-blind, blank- and tibolone-controlled study.

    abstract:BACKGROUND:As a Chinese Traditional Medicine product, Kuntai capsule could improve the peri-menopausal symptoms in postmenopausal women. But it is still not clear whether Kuntai capsule has a good effect on alleviating peri-menopausal symptoms induced by gonadotropin releasing hormone agonist (GnRH-a) treatment. The pu...

    journal_title:Chinese medical journal

    pub_type: 杂志文章,随机对照试验

    doi:10.4103/0366-6999.151057

    authors: Chen JM,Gao HY,Ding Y,Yuan X,Wang Q,Li Q,Jiang GH

    更新日期:2015-02-20 00:00:00

  • Effect of antimicrobial agents on the toll-like receptors and inflammatory cytokines in liver tissue of the alcohol-induced liver disease in rats with Vibrio vulnificus sepsis.

    abstract:BACKGROUND:Septicemia and inflammation-mediated septic shock caused by Vibrio vulnificus (VV) is strongly associated with chronic liver disease. This study examined the effects of antimicrobial therapy on expression of hepatic toll-like receptors and inflammatory cytokines in rats with alcohol-induced liver disease com...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Lu ZQ,Li MF,Qiu QM,Liang H,Zhou TL,Hong GL,Wu B

    更新日期:2009-08-20 00:00:00

  • Two novel STK11 mutations in three Chinese families with Peutz-Jeghers syndrome.

    abstract:BACKGROUND:Peutz-Jeghers syndrome (PJS) is an autosomal dominantly inherited disease. STK11/LKB1 gene germline mutations have been identified as responsible for PJS. In our study, we investigated the molecular basis of PJS and evaluated correlation between the STK11 mutations and the Chinese population. METHODS:We col...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:

    authors: Zuo YG,Xu KJ,Su B,Ho MG,Liu YH

    更新日期:2007-07-05 00:00:00

  • Surgical Outcomes of Anomalous Origin of the Left Coronary Artery from the Pulmonary Artery in Children: An Echocardiography Follow-up.

    abstract:BACKGROUND:Anomalous origin of the left coronary artery from the pulmonary artery (ALCAPA) is a rare but potentially life-threatening congenital heart defect. A retrospective analysis was carried out to elucidate the surgical outcomes of ALCAPA in infants and children using follow-up echocardiography. METHODS:From Sep...

    journal_title:Chinese medical journal

    pub_type: 杂志文章

    doi:10.4103/0366-6999.215334

    authors: Gao Y,Zhang J,Huang GY,Liang XC,Jia B,Ma XJ

    更新日期:2017-10-05 00:00:00

  • Circulatory responses to nasotracheal intubation: comparison of GlideScope videolaryngoscope and Macintosh direct laryngoscope.

    abstract:BACKGROUND:The GlideScope videolaryngoscope (GSVL) has been shown to have no special advantage over the Macintosh direct laryngoscope (MDL) in attenuating the circulatory responses to orotracheal intubation, but no study has compared the circulatory responses to nasotracheal intubation (NTI) using the two devices. This...

    journal_title:Chinese medical journal

    pub_type: 杂志文章,随机对照试验

    doi:

    authors: Xue FS,Li XY,Liu QJ,Liu HP,Yang QY,Xu YC,Liao X,Liu Y

    更新日期:2008-07-20 00:00:00