Abstract：
OBJECTIVE:To investigate the expression of Toll-like receptor 2 (TLR2) and Toll-like receptor 4 (TLR4) and the effect of lipopolysaccharide (LPS) on their expression in cultured endothelial cells. METHODS:Total RNA was extracted from ECV304 cells and isolated human umbilical vein endothelial cells (HUVECs) exposed to LPS, respectively. The quantification of TLR2 and TLR4 mRNA in HUVECs and EVC304 cells was carried out by reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS:ECV304 cells and HUVECs were able to express TLR2 and TLR4 mRNA, but the expression levels of TLR4 appeared to be stronger than those of TLR2. LPS could upregulate the expression levels of TLR4 obviously, whereas it had no effect on the expression level of TLR2. CONCLUSIONS:Our data indicate that TLR4 may be the LPS signal transducer in endothelial cells and plays important roles in the cell activation of LPS. The ECV304 cell line is a better experimental model than isolated HUVECs in the research of endothelial cells.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Yang Q,Zhu P,Wang Z,Jiang Jkeywords:
subject
Has Abstractpub_date
2002-02-01 00:00:00pages
286-9issue
2eissn
0366-6999issn
2542-5641journal_volume
115pub_type
杂志文章abstract：:Pancreatic neuroendocrine tumors (NETs) are one subgroup of gastroenteropancreatic NETs. Its main characteristics are slow growth, frequent metastasis to the liver, and limited to the liver for long periods. In patients with irresectable liver metastatic NET, liver transplantation is the only radical treatment. About ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-08-01 00:00:00
abstract：OBJECTIVE:To investigate changes in intestinal microflora in patients with chronic severe hepatitis (CSH), and their role in this life-threatening disease. METHODS:We classified nineteen patients with chronic severe hepatitis as the CSH group, thirty patients with chronic hepatitis (CH) as the CH group and thirty-one ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2001-08-01 00:00:00
abstract：OBJECTIVE:To clarify the role of these cyclins in human gastric cancer. METHODS:38 gastric cancer patients, 29 first degree relatives of gastric cancer patients, as well as 18 healthy subjects were included. The mRNA expression of cyclins D1, D2, D3 and E in gastric biopsies was evaluated by RT-PCR analysis using spec...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2002-05-01 00:00:00
abstract：BACKGROUND:The endovascular treatment of abdominal aortic aneurysm (AAA) has improved greatly in the last 15 years. The present study aimed to evaluate the endografting experience for the treatment of unfavorable abdominal aortic aneurysm (uAAA). METHODS:During December 2001 and December 2007, 41 patients with uAAA we...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-04-05 00:00:00
abstract：BACKGROUND:Alzheimer disease (AD) is a neurodegenerative disease related to aging. At present, its pathological mechanisms remain unclear. Family members of the renin-angiotensin system (RAS) play a role in neuronal plasticity, as well as formation of learning and memory. In this study, we explore the effects of altere...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2008-11-20 00:00:00
abstract：BACKGROUND:After establishing a murine model of aminoglycoside antibiotic (AmAn) induced ototoxicity, the sensitivity of AmAn induced ototoxicity in three murine strains and the effect of kanamycin on the expression of Na-K-2Cl cotransporter-1 (NKCC1) in stria vascularis were investigated. METHODS:C57BL/6J, CBA/CaJ, N...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2006-06-20 00:00:00
abstract：:Hemoglobin Jianghua (Hb Jianghua) is a new fast--moving hemoglobin variant found in China, being named after the county where it was detected. Having its primary structure analysed, we report in this paper the results of studies on its function. The absorption spectrum, response to allosteric effectors and oxygen equi...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：BACKGROUND:Selection of patients with hepatocellular carcinoma (HCC) for orthotopic liver transplantation (OLT) remains controversial. Since there is a trend to expand the transplant criteria for HCC patients, we reviewed the data of patients with HCC who had received OLT at our institute to determine their survival an...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2005-04-20 00:00:00
abstract：:102 patients with nasal polyps receiving ND: YAG laser surgery or routine surgery were followed up for 18-36 months. The postoperative recurrence rates were 40.6% (24/54) and 66.6% (32/48) respectively for the patients of the two groups. Laser surgery is marked by less bleeding, no postoperative packing of the nose an...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：OBJECTIVE:The P53 tumor suppressor gene shows the most frequent genetic alteration in human tumors. Mutation, deletion and rearrangement of P53 gene have been found in several types of neoplasm including osteosarcoma. The present study is to clarify the status of abnormalities of this gene in osteosarcoma in China. MA...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1996-10-01 00:00:00
abstract：:Four calcium channel blockers, i.e. nifedipine, verapamil, cinnarizine and tetrandrine are currently available and used widely in treating cardiovascular diseases. To confirm the effects, if any, of calcium channel blockers on cirrhotic patients with portal hypertension, a study was performed on esophageal variceal pr...
journal_title：Chinese medical journal
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1995-11-01 00:00:00
abstract：OBJECTIVE:To investigate the feasibility of adrenal sparing surgery for selected renal cell carcinoma (RCC), since the necessity of routine adrenalectomy during radical nephrectomy for RCC has been challenged in recent years. METHODS:178 patients underwent perifascial nephrectomy in a 16-year period. Of these patients...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
abstract：:By employing monoclonal phosphorylated neurofilaments antibody, we studied the abnormally pale-staining neurons in 3 cases of corticonigral degeneration by the avidin-biotin immunoperoxidase method. For comparison, the central chromatolysis in anterior horn cells secondary to cervical spine fracture and "ballooned" ne...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：OBJECTIVE:To report the surgical techniques and results of an 8-year follow-up study of 42 patients with a modified orthotopic ileal neobladder restoring normal anatomical relationship. METHODS:Total cystoprostatectomy was performed extraperitoneally. A 45 - 50 cm segment of the ileal loop was isolated, detubularized,...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
abstract：BACKGROUND:Chronic obstructive pulmonary disease (COPD) is thought to be an inflammatory immune response disease. In most cases, the disease is caused by cigarette smoke, but it has been demonstrated that only 10% to 20% of smokers will definitely suffer from COPD. Dendritic cells (DCs) are considered to be the promote...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-06-01 00:00:00
abstract：BACKGROUND:Ulcerative colitis (UC) is associated with differential expression of genes involved in inflammation and tissue remodeling. MicroRNA (miRNA) plays an important role in the pathogenesis of UC by regulating the gene expression at the post-transcriptional level and control crucial physiological processes. This ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:The mechanisms underlying postoperative pain remain unclear. Neurotransmitters of excitatory and inhibitory amino acids play an important role in the transmission and modulation of pain in the spinal dorsal horn. This study aimed to investigate the changes of release of excitatory and inhibitory amino acids ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2007-11-20 00:00:00
abstract：BACKGROUND:It is important to determine prognostic factors for the outcome of amyotrophic lateral sclerosis (ALS) at an early stage. The time taken for symptoms to spread from spinal or bulbar regions to both (time to generalization; TTG) is considered a strong predictor of survival; however, this has rarely been studi...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000000200
更新日期：2019-05-05 00:00:00
abstract：BACKGROUND:Adenoid hypertrophy (AH) is associated with pediatric chronic rhinosinusitis (pCRS), but its role in the inflammatory process of pCRS is unclear. It is thought that innate immunity gene expression is disrupted in the epithelium of patients with chronic rhinosinusitis (CRS), including antimicrobial peptides a...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.168056
更新日期：2015-11-05 00:00:00
abstract：BACKGROUND:Susceptibility to coronary artery disease (CAD) and diabetes is encoded by distinct, tightly-linked single nucleotide polymorphisms on chromosome 9p21. This study aimed to examine the association of variant rs1333049 on chromosome 9p21.3 with early-onset and severity of CAD in Chinese patients with and witho...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.3901/jme.2011.08.066
更新日期：2011-01-01 00:00:00
abstract：BACKGROUND:Whether antibody to myelin oligodendrocyte glycoprotein (MOG) can be a diagnostic marker for multiple sclerosis (MS) is still controversial. Recent studies suggested that serum specific anti-MOG epitope antibody might be an MS specific marker. However, these studies did not include neuromyelitis optica (NMO)...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-09-01 00:00:00
abstract：BACKGROUND:There are few reports of microsurgical treatment of dorsum sellae meningiomas-which, because of location, size, and differences in growth direction, clinical presentations, degree of surgical difficulty, have varied posttreatment sequelae. In pursuit of an optimal microsurgical treatment option for dorsum se...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-08-20 00:00:00
abstract：BACKGROUND:The long-term safety and efficacy of drug-eluting stents (DES) versus bare metal stents (BMS) are unclear and controversial issues in patients with acute ST-elevation myocardial infarction (STEMI) undergoing primary percutaneous coronary intervention (PCI). The purpose of this study was to compare the long-t...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-08-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to demonstrate the lumbar pedicle cortical bone trajectory (CBT) screw fixation technique, a new fixation technique for lumbar surgery. DATA SOURCES:The data analyzed in this review are mainly from articles reported in PubMed published from 1994 to 2014. STUDY SELECTION:Origina...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:The change of glucose transporter 4 (GLUT4) expression could influence glucose uptake in the myocardial cells and then effect myocardial metabolism, which maybe one of the factor for the diabetes cardiovascular disease. This study aimed to explore the influence of glucose and insulin at different concentrati...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：: ...
journal_title：Chinese medical journal
pub_type： 社论
doi：10.4103/0366-6999.247217
更新日期：2018-12-20 00:00:00
abstract：OBJECTIVE:To investigate injury to epidermal melanocyte by Q-switched Alexandrite laser. METHODS:Multiple biopsies were performed on 5 patients with nevus of Ota from before irradiation to 1 year after irradiation. Fourteen specimens were obtained for light microscopy, and 17 for transmission electron microscopy. RES...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2003-04-01 00:00:00
abstract：BACKGROUND:Cervical arthroplasty is indicated to preserve cervical motion and prevent accelerated adjacent segment degeneration. Whether accelerated adjacent segment degeneration is prevented in the long term is unclear. This trial compared adjacent segment degeneration in Bryan disc arthroplasty with that in anterior ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-11-01 00:00:00
abstract：BACKGROUND:The most important objective of transplant studies in the injured spinal cord has been to provide a favorable environment for axonal growth. Moreover, the continuing discovery of new grafts is providing new potentially interesting transplant candidates. Our purpose was to observe the morphological and functi...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
abstract：Background:Development of innovative immunotherapy is imperative to improve the poor survival of the nasopharyngeal carcinoma (NPC) patients. In this study, we evaluated the T cell response to melanoma-associated antigen (MAGE)-A1, MAGE-A3, or synovial sarcoma X-2 (SSX-2) in the peripheral blood of treatment-naive NPC ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.232791
更新日期：2018-06-05 00:00:00
