Abstract:
Background:Development of innovative immunotherapy is imperative to improve the poor survival of the nasopharyngeal carcinoma (NPC) patients. In this study, we evaluated the T cell response to melanoma-associated antigen (MAGE)-A1, MAGE-A3, or synovial sarcoma X-2 (SSX-2) in the peripheral blood of treatment-naive NPC patients. The relationship of responses among the three proteins and the human leukocyte antigen (HLA)-A types were analyzed to provide evidence of designing novel therapy. Methods:Sixty-one NPC patients admitted into the Tumor Hospital affiliated to the Xinjiang Medical University between March 2015 and July 2016 were enrolled. Mononuclear cells were isolated from the peripheral blood before any treatment. HLA-A alleles were typed with Sanger sequence-based typing technique. The T cell response to the MAGE-A1, MAGE-A3, or SSX-2 was evaluated with the Enzyme-Linked ImmunoSpot assay. Mann-Whitney U-test was used to compare the T cell responses from different groups. Spearman's rank correlation was used to analyze the relationship of T cell responses. Results:HLA-A*02:01, A*02:07, and A*24:02 were the three most frequent alleles (18.9%, 12.3%, and 11.5%, respectively) among the 22 detected alleles. 31.1%, 19.7%, and 16.4% of the patients displayed MAGE-A1, MAGE-A3, or SSX-2-specific T cell response, respectively. The magnitudes of response to the three proteins were 32.5, 38.0, and 28.7 SFC/106 peripheral blood mononuclear cells, respectively. The T cell response against the three proteins correlated with each other to different extent. The percentage of A*02:01 and A*24:02 carriers were significantly higher in patients responding to any of the three proteins compared to the nonresponders. Conclusion:MAGE-A1, MAGE-A3, or SSX-2-specific T cell responses were detectable in a subgroup of NPC patients, the frequency and magnitude of which were correlated. :中晚期鼻咽癌患者外周血中HLA-A基因限制性MAGE-A1、MAGE-A3及SSX-2蛋白诱导的T细胞免疫反应初探摘要背景:局部复发和远处转移是鼻咽癌(nasopharyngeal carcinoma, NPC)治疗失败的主要原因,严重影响患者的生存,寻找新的免疫治疗方案意义重大。本研究探讨中晚期NPC初治患者外周血中HLA A限制性MAGE A1、A3及SSX 2抗原肽细胞毒性T细胞(CTL)免疫反应水平,并探讨三者之间的内在联系,为中晚期NPC患者细胞免疫治疗提供实验依据。 方法:选择2015年3月~2016年7月新疆医科大学附属肿瘤医院由病理明确诊断的初治中晚期NPC患者61例,采用SBT sanger法检测患者HLA A等位基因单倍体分型;采用酶联免疫斑点法(ELISPOT)检测经MAGE A1、SSX 2抗原肽刺激后的PBMC分泌IFN γ的CTL细胞的频率及强度。采用Mann Whitney U秩和检验分析患者组间MAGE A1、 MAGE A3、SSX 2反应强度差异,用Spearman's rank correlation进行相关性分析。 结果:61例患者共检测出HLA A等位基因分型22种,其中基因频率前三位的分别是A*02:01(18.9%),A*02:07(12.3%)以及A*24:02(11.5%)。鼻咽癌患者外周血中MAGE A1、MAGE A3及SSX 2抗原特异性CTL免疫应答阳性率分别为31.1%,19.7%和16.4%;平均反应强度分别为32.5,38.0及28.7 SFC/106 PBMC。MAGE A1、MAGE A3与SSX 2之间CTL反应频率与强度均成正相关。MAGE A1、MAGE A3及SSX 2特异性抗原肽阳性反应患者中携带A*02:01(21.1%, 20.8%和25.0%)和A*24:02(18.4%, 25.0%和25.0%)等位基因型的频率较高。 结论:中晚期NPC患者可诱导HLA A限制性MAGE A1、MAGE A3与SSX 2抗原特异性T细胞免疫反应,且彼此间频率与强度具有一定关联性,可能为中晚期NPC患者免疫治疗的临床应用提供实验室依据。.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Fan PW,Huang L,Chang XM,Feng YN,Yao X,Peng YC,Dong T,Wang RZdoi
10.4103/0366-6999.232791subject
Has Abstractpub_date
2018-06-05 00:00:00pages
1289-1295issue
11eissn
0366-6999issn
2542-5641pii
ChinMedJ_2018_131_11_1289_232791journal_volume
131pub_type
杂志文章abstract::The effects of the extracts of 20 Chinese medicinal herbs and an antiviral drug foscarnet on duck hepatitis B virus (DHBV) endogenous DNA polymerase (DNAp) activity were compared. The extracts of P. urinaria showed a dose-dependent inhibition on DHBV DNAp. And those of other herbs showed little inhibition effect. Prim...
journal_title:Chinese medical journal
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更新日期:1995-09-01 00:00:00
abstract:BACKGROUND:The acute abdomen remains a challenge for all obstetricians and physicians who take part in the care of women in pregnancy. To add substantially to our understanding of acute pancreatitis (AP) in pregnancy, in particular affirming the increased risks for mother and fetus associated with AP, we explored featu...
journal_title:Chinese medical journal
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更新日期:2011-09-01 00:00:00
abstract:BACKGROUND:Receptor activator of nuclear factor kappa B (NF-κB) ligand (RANKL) and osteoprotegerin (OPG) have been recently shown to play important roles in bone resorption. The aim of this study was to investigate the possible association between the expression of bone resorption regulators (RANKL and OPG) and inflamm...
journal_title:Chinese medical journal
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更新日期:2011-07-01 00:00:00
abstract:BACKGROUND:The rapid transmission and high mortality rate made severe acute respiratory syndrome (SARS) a global threat for which no efficacious therapy is available now. Without sufficient knowledge about the SARS coronavirus (SARS-CoV), it is impossible to define the candidate for the anti-SARS targets. The putative ...
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abstract:BACKGROUND:Premature ventricular contractions (PVCs) are common in the general population, and frequent PVCs may result in the poor quality of life or even the damage of cardiac function. We examined the efficacy and safety of a traditional Chinese medicine Wenxin Keli for the treatment of frequent PVCs among a relativ...
journal_title:Chinese medical journal
pub_type: 杂志文章,多中心研究,随机对照试验
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abstract::The effect of harringtonine (HA) in various concentrations on HL-60 was evaluated. 0.10 microgram/ml HA showed a strong cell killing effect, and 0.025 microgram/ml HA a weak differentiation induction accompanied by cell growth retardation. Differentiation induction did not occur in 5 other human leukemic cell lines. W...
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abstract:BACKGROUND:Estrogen receptor (ER)-negative breast cancer cells are more aggressive than ER-positive cells. Elevated levels of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor-C (VEGF-C) expression have been detected in cultured human breast cancer cells and are associated with negative hormone receptor s...
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更新日期:2010-08-05 00:00:00
abstract:BACKGROUND:Studies have shown that β-blockers can improve cardiac performance in heart failure (HF) by reversing protein kinase A (PKA)-mediated sarcoplasmic reticulum (SR) Ca2+ leak. However, it is being strongly questioned as to whether the PKA-mediated ryanodine receptor (RyR2) hyper-phosphorylation is a critical re...
journal_title:Chinese medical journal
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更新日期:2012-03-01 00:00:00
abstract:BACKGROUND:Contrast-enhanced ultrasound is a dynamic and continuous modality providing real-time view of vascularization and flow distribution patterns of different organs and tumors. In order to evaluate the diagnostic significance of intraoperative contrast-enhanced ultrasound in assessing the resection degree of bra...
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pub_type: 杂志文章
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abstract:: ...
journal_title:Chinese medical journal
pub_type: 信件
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journal_title:Chinese medical journal
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doi:
更新日期:2003-01-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2008-02-20 00:00:00
abstract:OBJECTIVES:To study the interrelation between nitric oxide (NO) and endothelin-1 (ET1) in experimental acute hypoxic rats, and to evaluate the mechanism of acute hypoxic pulmonary hypertension affected by NO and ET1 and its intervention. METHODS:Nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemi...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract:OBJECTIVE:Dermoscopy is a useful technique for improving the diagnostic accuracy of various types of skin disorders. In China, dermoscopy has been widely accepted, and domestic researchers have made tremendous progress in the field of dermoscopy. The main purpose of this review is to summarize the current status of der...
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pub_type: 杂志文章,评审
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更新日期:2019-09-05 00:00:00
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pub_type: 杂志文章
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.168040
更新日期:2015-11-05 00:00:00
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pub_type: 杂志文章
doi:
更新日期:2011-06-01 00:00:00
abstract::102 patients with nasal polyps receiving ND: YAG laser surgery or routine surgery were followed up for 18-36 months. The postoperative recurrence rates were 40.6% (24/54) and 66.6% (32/48) respectively for the patients of the two groups. Laser surgery is marked by less bleeding, no postoperative packing of the nose an...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract:BACKGROUND:The earthworm fibrinolytic enzyme (EFE) is a complex protein enzyme that is widely distributed in the earthworm's digestive cavity. Possessing strong protein hydrolysis activity, EFE not only has a direct effect on fibrin, but also can activate plasminogen. Its therapeutic and preventative effects on thrombo...
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pub_type: 杂志文章
doi:
更新日期:2007-05-20 00:00:00
abstract:BACKGROUND:Restrictive cardiomyopathy (RCM) is the least common cardiomyopathy in which the walls are rigid and the heart is restricted from stretching and filling properly. Cardiac troponin I (cTnI) mutation-caused myofibril Ca2+ hypersensitivity has been shown to be associated with impaired diastolic function. This s...
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pub_type: 杂志文章
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doi:
更新日期:2013-11-01 00:00:00
abstract:BACKGROUND:Although breast-conserving surgery is one of the standard treatments for breast cancer, few studies have assessed its recent implementation in China. We aimed to clarify the current real-world status of breast-conserving surgery in China. METHODS:This cross-sectional survey relied on data collected by the C...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000001152
更新日期:2020-11-20 00:00:00
abstract:BACKGROUND:Irritable bowel syndrome (IBS) is reported associated with the alteration of gut microbial composition termed as dysbiosis. However, the pathogenic mechanism of IBS remains unclear, while the studies of Chinese individuals are scarce. This study aimed to understand the concept of dysbiosis among patients wit...
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pub_type: 杂志文章
doi:10.1097/CM9.0000000000000192
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abstract:BACKGROUND:Orthotopic liver transplantation (OLT) has become the therapeutic option of choice for end-stage liver disease. The aim of this study was to investigate the changes of splenic morphology, signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), apparent diffusion coefficient (ADC) values and explore their...
journal_title:Chinese medical journal
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:To examine the effect of selective head cooling (SHC) on brain cell membrane activity involving ATPase, phospholipase A2, content of total membrane phospholipids during postischemic reperfusion, so as to elucidate the possible underlying mechanism on resuscitating effect of SHC. MATERIALS AND METHODS:Complet...
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doi:
更新日期:1996-06-01 00:00:00
abstract::A rare type of annular pancreas, in which the tail of the annular duct is fused with the dorsal pancreatic duct to form the main duct, and its diagnostic imagings of endoscopic retrograde cholangiopancreatography, hypotonic duodenography, computed tomography and ultrasound are described. Annular pancreas is an anomaly...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract:BACKGROUND:Morphologic imaging after radiofrequency ablation (RFA) of liver metastases is hampered by an inflammatory response in the ablation margin, making the identification of local tumor progression (LTP) difficult. The aim of this study was to evaluate the efficacy of early (18)F-FDG PET/CT scanning to monitor th...
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pub_type: 杂志文章
doi:
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND:Previous studies demonstrated general anesthetics affect potassium ion channels, which may be one of the mechanisms of general anesthesia. Because the effect of etomidate on potassium channels in rat hippocampus which is involved in memory function has not been studied, we investigated the effects of etomida...
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更新日期:2010-03-20 00:00:00
abstract:Objective:Endothelial cells (ECs) are important metabolic and endocrinal organs which play a significant role in regulating vascular function. Vascular ECs, located between the blood and vascular tissues, can not only complete the metabolism of blood and interstitial fluid but also synthesize and secrete a variety of b...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
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