Abstract：
AIM:To explore the virulence and the potential pathogenicity of coccoid Helicobacter pylori (H. pylori) transformed from spiral form by exposure to antibiotic. METHODS:Three strains of H. pylori, isolated from gastric biopsy specimens of confirmed peptic ulcer, were converted from spiral into coccoid from by exposure to metronidazole. Both spiral and coccoid form of H. pylori were tested for the urease activity, the adherence to Hep-2 cells and the vacuolating cytotoxicity to Hela cells, and the differences of the protein were analysed by SDS-PAGE and Western blot. The mutation of the genes including ureA, ureB,hpaA, vacA and cagA, related with virulence, was detected by means of PCR and PCR-SSCP. RESULTS:In the coccoid H. pylori,the urease activity, the adherence to Hep-2 cells and the vacuolating cytotoxicity to Hela cells all decreased. In strain F44, the rate and index of adherence reduced from 70.0% +/- 5.3% to 33% +/- 5.1% and from 2.6 +/- 0.4 to 0.96 +/- 0.3 (P < 0.01), respectively. The invasion of coccoid H. pylori into Hep-2 cell could be seen under electronmicroscope. SDS-PAGE showed that the content of the protein with the molecular weight over Mr 74000 decreased, and the hybriditional signal in band M(r) 125000 weakened, while the band M(r)110000 and M(r)63000 strengthened in coccoid H.pylori as shown in Western blot. The results of PCR were all positive, and PCR-SSCP indicated that there may exist the point mutation in gene hpaA or vacA. CONCLUSION:The virulence and the proteins with molecular weight over M(r)74000 in coccoid H.pylori decrease, but no deletion exists in amplification fragments from ureA, ureB, hpaA, vacA and cagA genes, suggesting that coccoid H.pylori may have potential pathogenicity.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
She FF,Su DH,Lin JY,Zhou LYdoi
10.3748/wjg.v7.i2.254keywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
254-8issue
2eissn
1007-9327issn
2219-2840journal_volume
7pub_type
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journal_title：World journal of gastroenterology
pub_type： 杂志文章,评审
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journal_title：World journal of gastroenterology
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abstract：AIM:To investigate the expression frequency of endocan in colorectal cancer and analyze the relationship between endocan expression and clinical parameters and to study the role of endocan in colorectal carcinogenesis. METHODS:Expression of endocan in 72 tumor tissue samples of colorectal cancer as well as in 27 norma...
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pub_type： 杂志文章
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journal_title：World journal of gastroenterology
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journal_title：World journal of gastroenterology
pub_type： 杂志文章,评审
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journal_title：World journal of gastroenterology
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pub_type： 杂志文章,meta分析,评审
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v21.i22.6931
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journal_title：World journal of gastroenterology
pub_type： 杂志文章,随机对照试验
doi：10.3748/wjg.v13.i46.6259
更新日期：2007-12-14 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v26.i47.7497
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
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journal_title：World journal of gastroenterology
pub_type： 杂志文章,多中心研究,随机对照试验
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pub_type： 杂志文章,评审
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journal_title：World journal of gastroenterology
pub_type： 杂志文章,评审
doi：10.3748/wjg.v20.i46.17297
更新日期：2014-12-14 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章,评审
doi：10.3748/wjg.v20.i14.3905
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journal_title：World journal of gastroenterology
pub_type： 杂志文章,评审
doi：10.3748/wjg.v20.i28.9270
更新日期：2014-07-28 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v11.i30.4735
更新日期：2005-08-14 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
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journal_title：World journal of gastroenterology
pub_type： 杂志文章,评审
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journal_title：World journal of gastroenterology
pub_type： 临床试验,杂志文章
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更新日期：2007-10-28 00:00:00
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journal_title：World journal of gastroenterology
pub_type： 杂志文章
doi：10.3748/wjg.v23.i42.7609
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journal_title：World journal of gastroenterology
pub_type： 社论
doi：10.3748/wjg.v21.i34.9833
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abstract：BACKGROUND:Extrahepatic metastasis (EHM) of hepatocellular carcinoma (HCC) is associated with poor outcomes. However, the clinical features and risk factors of EHM of HCC after radiofrequency ablation (RFA) remain unclear. AIM:To elucidate the characteristics and risk factors of EHM after RFA for HCC. METHODS:From Ja...
journal_title：World journal of gastroenterology
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