Abstract：
:To understand spatiotemporally coordinated activity in neural networks and interaction between different areas or layers in brain tissue, simultaneous multisite recording is a prerequisite. For in vitro studies pursuing these goals, substrate integrated, planar microelectrode arrays (MEAs) have been developed to monitor spikes and local field potentials. Here we report for the first time recordings of single-unit spike activity with MEAs in acute slice preparations of the rat cerebellum. We compare these recordings to results of conventional techniques, and discuss the recording conditions in view of the equivalent circuits commonly used. Simultaneous recordings with tungsten microelectrodes and MEAs verified that recording characteristics and signal-to-noise ratios of MEA electrodes were comparable to those of conventional extracellular electrodes. Spike shapes were identical on both electrodes. We found no detectable overlap between spike signals recorded at neighboring MEA electrodes (200 microm spacing). Neuronal spike activity was detected with MEA electrodes at distances of up to 100 microm from the site of spike generation. We conclude that extracellular recording of independent single-unit spike activity with MEAs is indeed suitable to monitor network activity in acute slices, making MEAs an exceptionally useful tool for the assessment of fast network dynamics in acute slices.
journal_name
Exp Brain Resjournal_title
Experimental brain researchauthors
Egert U,Heck D,Aertsen Adoi
10.1007/s00221-001-0932-5keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
268-74issue
2eissn
0014-4819issn
1432-1106journal_volume
142pub_type
杂志文章abstract：:The aim of the present study was to further investigate the contribution of primary muscle spindle feedback to proprioception and higher brain functions, such as movement trajectory recognition. For this purpose, complex illusory movements were evoked in subjects by applying patterns of muscle tendon vibration mimicki...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-005-0325-2
更新日期：2006-06-01 00:00:00
abstract：:Perhaps the most intriguing disorders of body representation are those that are not due to primary disease of brain tissue. Strange and sometimes painful phantom limb sensations can result from loss of afference to the brain; and Complex Regional Pain Syndrome (CRPS)-the subject of the current report-can follow limb t...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-009-2107-8
更新日期：2010-07-01 00:00:00
abstract：:We recognize objects even when they are partially degraded by visual noise. Using monkeys performing a sequential delayed match-to-sample task, we studied the relation between the amount of visual noise (5, 10, 15, 20 or 25%) degrading the eight black and white stimuli used here, and the accuracy and speed with which ...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-005-2230-0
更新日期：2005-05-01 00:00:00
abstract：:Reflexive attentional shift in response to another individual's gaze direction has been reported, but it remains unknown whether this process can occur subliminally. We investigated this issue using facial stimuli consisting of drawings (Experiment 1) and photographs (Experiment 2). The gaze direction was expressed by...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-007-1025-x
更新日期：2007-10-01 00:00:00
abstract：:Chemoreceptor pathways from the vomeronasal organ (VNO), and main olfactory system are known to be separate as they pass into the brain, at least until the level of the amygdala. In the amygdala, vomeronasal pathways project to the posteromedial cortical nucleus (PMCN), and medial nucleus (MN). The main olfactory path...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/BF00247024
更新日期：1987-01-01 00:00:00
abstract：:The luminance detectors in the olivary pretectal nucleus, which are likely candidates mediating the pupillary light reflex, responded to all frequencies of sinusoidally modulated light up to 12-25 Hz. At low frequencies (0.05-4.0 Hz) the luminance detectors responded with modulated firing to different stimulation rate...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/BF00237669
更新日期：1985-01-01 00:00:00
abstract：:Previous studies have shown that rearing rabbits in a stroboscopically illuminated environment results in a decrease in orientation and direction selectivity and an increase in responsivity to stroboscopic stimuli among neurons in area 17. In the present study, the critical period for susceptibility to these effects w...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/BF00239202
更新日期：1983-01-01 00:00:00
abstract：:Lightly touching a stable reference is associated with sway reduction during standing. Unexpected displacement of the touch reference results in a false-positive balance reaction in some participants, but only with the first such disturbance. This study investigated whether light touch reduces standing sway (1) after ...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-018-5455-4
更新日期：2019-03-01 00:00:00
abstract：:In this study, we investigated the neural basis of virtual time to contact (VTC) and the hypothesis that VTC provides predictive information for future postural instability. A novel approach to differentiate stable pre-falling and transition-to-instability stages within a single postural trial while a subject was perf...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-009-1956-5
更新日期：2009-10-01 00:00:00
abstract：:The purpose of the present work was to characterize calcium responses of brain-capillary endothelial cells (BCEC), the cells forming the blood-brain barrier, to chemical, hyperosmolar and mechanical stimulation. Confluent BCEC cultures were grown from capillary fragments isolated from rat cerebral cortex. Intracellula...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s002210050755
更新日期：1999-06-01 00:00:00
abstract：:The executive functions, which depend on the adequate maturation and functioning of the prefrontal cortex and its connection to posterior zones, follow a process of development as age increases. This work studied changes in the absolute power (AP) of EEG activity recorded in the prefrontal and parietal areas during th...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-013-3693-z
更新日期：2013-11-01 00:00:00
abstract：:The time course of 72-kDa heat-shock protein (hsp72) induction was evaluated by immunoblotting in cerebral cortex, striatum, hippocampus, cerebellum, liver, and kidney of rats subjected to 60-min focal cerebral ischemia following proximal unilateral occlusion of the right middle cerebral artery (MCA). Neurological exa...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s002210000564
更新日期：2001-01-01 00:00:00
abstract：:The responses of 80 amygdaloid neurons to the four basic taste (sucrose, NaCl, HCl and quinine hydrochloride), thermal (5 degrees C, 20 degrees C and 40 degrees C) and tactile (brushing) stimuli applied to the anterior part of the tongue were recorded in anesthetized rats. About 90% of the taste-sensitive amygdaloid n...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/BF00237437
更新日期：1984-01-01 00:00:00
abstract：:Postsynaptic potentials (PSPs) evoked in motoneurons innervating the back and abdominal muscles in the lumbar part of the body by stimulating hindlimb cutaneous afferents were investigated in unanesthetized decerebate and spinal cats. Various types of PSP: pure excitatory postsynaptic potential (EPSP), pure inhibitory...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s002210050879
更新日期：1999-10-01 00:00:00
abstract：:Postural control is an important factor for early motor development; however, compared with adults, little is known about how infants control their unperturbed upright posture. This lack of knowledge, particularly with respect to spatial and temporal characteristics of infants' unperturbed independent standing, repres...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-007-1236-1
更新日期：2008-03-01 00:00:00
abstract：:In dystonia, both sensory malfunctioning and an abnormal intermuscular low-frequency drive of 3-7 Hz have been found, although cause and effect are unknown. It is hypothesized that sensory processing is primarily disturbed and induces this drive. Accordingly, experimenter-controlled sensory input should be able to inf...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-010-2174-x
更新日期：2010-05-01 00:00:00
abstract：:It is known that kittens reared in ways that restrict movement while visual stimulation is received exhibit deficits in visually guided behavior. Presumably, the behavioral dysfunction is due to a lack of sensorimotor coordination during visual exposure. The current investigation was undertaken to study this effect bo...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/BF00238794
更新日期：1978-09-15 00:00:00
abstract：:Many studies have demonstrated attenuated verbal working memory (WM) under articulatory suppression. However, performance is not completely abolished, suggesting a less efficient, non-articulatory mechanism for the maintenance of verbal information. The neural causes for the reduced efficiency of such a putative compl...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-013-3770-3
更新日期：2014-02-01 00:00:00
abstract：:The neural activity in area 7a and the arm area of motor cortex was recorded while real or path-guided apparent motion stimuli were presented to behaving monkeys in the absence of a motor response. A smooth stimulus motion was produced in the real motion condition, whereas in the apparent motion condition five stimuli...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-003-1664-5
更新日期：2004-02-01 00:00:00
abstract：:We investigated in sheep the effects of stimulation of the thick afferent fibers running through the lingual nerve (LN) upon the activity of some of the muscles and medullary interneurons or motoneurons which are active during swallowing. Using electromyography (EMG), and extra- and intracellular neuronal recording, w...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s002210000361
更新日期：2000-06-01 00:00:00
abstract：:It has long been assumed that the human central nervous system uses flexible combinations of several muscle synergies to effortlessly and efficiently control redundant movements. However, whether muscle synergies exist in the neural circuit remains controversial, and it is critical to examine the association between t...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-015-4253-5
更新日期：2015-06-01 00:00:00
abstract：:In 1995, an aftereffect following treadmill running was described, in which people would inadvertently advance when attempting to run in place on solid ground with their eyes closed. Although originally induced from treadmill running, the running-in-place after-effect is argued here to result from the absence of senso...
journal_title：Experimental brain research
pub_type： 临床试验,杂志文章
doi：10.1007/s002210050769
更新日期：1999-07-01 00:00:00
abstract：:The purposes of this pilot study were to create a model of focal cortical ischemia in Macaca fascicularis and to explore contributions of the reticulospinal system in recovery of reaching. Endothelin-1 was used to create a focal lesion in the shoulder/elbow representation of left primary motor cortex (M1) of two adult...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-015-4390-x
更新日期：2015-11-01 00:00:00
abstract：:Current studies show that type II glucocorticoid receptor (GR) stimulation during recurring insulin-induced hypoglycemia (RIIH) results in diminished hypoglycemic activation of neurons in discrete CNS metabolic structures, namely the lateral hypothalamic area (LHA), hypothalamic paraventricular (PVH) and dorsomedial (...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-006-0614-4
更新日期：2007-01-01 00:00:00
abstract：:The topographical mapping of glial fibrillary acidic protein (GFAP)-immunoreactivity was performed in coronal serial sections of the rat mesencephalon, rhombencephalon and spinal cord. Relative to a background of poor or moderate overall staining of the mesencephalon, the interpeduncular nucleus, substantia nigra and ...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/BF00230695
更新日期：1989-01-01 00:00:00
abstract：:Grasping using two forelimbs in opposition to one another is evolutionary older than the hand with an opposable thumb (Whishaw and Coles in Behav Brain Res 77:135-148, 1996); yet, the mechanisms for bimanual grasps remain unclear. Similar to unimanual grasping, the localization of matching stable grasp points on an ob...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-013-3643-9
更新日期：2013-10-01 00:00:00
abstract：:In 15 musicians, cortical DC-potentials were recorded from the scalp before and during the execution of bimanual motor sequences. Subjects (Ss) either tapped with their two index fingers in synchrony (quavers against quavers; "2 against 2") or they tapped quavers against triplets ("2 against 3"). Either the right or t...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/BF00229320
更新日期：1990-01-01 00:00:00
abstract：:Traditional approaches to research on bimanual coordination focus on sensorimotor interference, motor programming, and effects of perception and feedback guidance; surprisingly, little is known about high-level conceptual constraints that might unify separate movements into coordinated actions. We investigated two pos...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-010-2365-5
更新日期：2010-08-01 00:00:00
abstract：:We have developed a tri-axial model of spatial orientation applicable to static 1g and non-1g environments. The model attempts to capture the mechanics of otolith organ transduction of static linear forces and the perceptual computations performed on these sensor signals to yield subjective orientation of the vertical...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-006-0387-9
更新日期：2006-08-01 00:00:00
abstract：:Normal vision overrides perturbed vestibular information for the optimization of performance during goal directed locomotion, suggesting down-regulation of vestibular gain. However, it is not known if the responses to vestibular perturbation are accentuated when vision is impaired. Furthermore, both visual and vestibu...
journal_title：Experimental brain research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00221-006-0593-5
更新日期：2007-01-01 00:00:00
