Abstract：
:Spinocerebellar ataxia type 2 is caused by a polyglutamine stretch in the protein ataxin-2 that is due to an expansion of a CAG repeat in the spinocerebellar ataxia-2 gene. The function of wild-type ataxin-2 has not been clarified. A widespread distribution of this protein throughout the brain has been reported. We examined the expression of ataxin-2 in cortical cerebellar cells of the adult rat. We performed a single label immunohistochemical study of ataxin-2 and a single label immunofluorescence study of ataxin-2 and zebrin on adjacent sections, to compare the distribution of the observed parasagittal band pattern. We also performed a double label immunofluorescence study of ataxin-2 and one of each parvalbumin, calbindin, and calretinin. Single label studies revealed that between 50% and 70% of the Purkinje cells express ataxin-2. The abundance of ataxin-2 was different between hemisphere and vermis, with a clear prevalence for the former. Furthermore, the distribution of ataxin-2-positive Purkinje cells showed a peculiar alternating parasagittal band pattern. Among the other cortical cerebellar cells only basket and granule cells showed ataxin-2 staining. Our dual label studies showed that about 50% of calbindin and more than 70% of parvalbumin-immunoreactive Purkinje cells were also labeled for ataxin-2. The uneven distribution of ataxin-2 expression in the Purkinje cell layer does not support the hypothesized link between ataxin-2 content and cell vulnerability. The differences in ataxin-2 expression among the cell types of cerebellar cortex, on the other hand, suggest a possible correlation between ataxin-2 content and cell function.
journal_name
Brain Res Bulljournal_title
Brain research bulletinauthors
Fusco FR,Viscomi MT,Bernardi G,Molinari Mdoi
10.1016/s0361-9230(01)00601-3keywords:
subject
Has Abstractpub_date
2001-10-01 00:00:00pages
343-7issue
3-4eissn
0361-9230issn
1873-2747pii
S0361-9230(01)00601-3journal_volume
56pub_type
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更新日期：1994-01-01 00:00:00
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更新日期：1992-07-01 00:00:00
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更新日期：1991-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：1986-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：1994-01-01 00:00:00
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journal_title：Brain research bulletin
pub_type： 杂志文章,评审
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更新日期：2007-04-30 00:00:00
