Abstract：
:Murine serum amyloid A1.1 (SAA1.1) has been conjugated with the fluorophore Texas Red (TxR), and its interaction with peritoneal macrophages has been visualized by scanning confocal microscopy. Binding of TxR-SAA to cell surfaces was inhibited by an excess of unlabelled SAA indicating the involvement of saturable receptors. Internalized TxR-SAA was seen initially as small punctate signals which in some cells evolved into a fine fluorescent network, a pattern typical of tubular endosomes. Colocalization of TxR-SAA with Cy5-labelled low density lipoprotein (LDL) but not with Oregon Green-labelled transferrin suggested that SAA trafficked through endosomes and lysosomes for degradation rather than through recycling compartments. Consistent with this catabolic pathway, macrophages loaded with TxR-SAA lost fluorescence within several days after being shifted to a fluorophore-free medium. In sharp contrast to this, cells maintained under amyloid-forming conditions, i.e. in the presence of unlabelled SAA and amyloid-enhancing factor (AEF) before and after treatment with TxR-SAA, remained brightly fluorescent over the course of 5 days. Immunocytochemistry verified the accumulation of SAA within macrophages. These findings support the hypothesis that a decreased catabolism of internalized SAA plays a role in AA amyloid pathogenesis.
journal_name
Scand J Immunoljournal_title
Scandinavian journal of immunologyauthors
Kluve-Beckerman B,Manaloor J,Liepnieks JJdoi
10.1046/j.1365-3083.2001.00879.xkeywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
393-400issue
4eissn
0300-9475issn
1365-3083pii
sji879journal_volume
53pub_type
杂志文章abstract：:Epstein-Barr virus (EBV)-transformed human B cells were seeded at densities below their capacity for autostimulatory growth and the effects of interleukin 1-alpha (IL-1-alpha), interleukin 1-beta (IL-1-beta) and B-cell growth factor (BCGF) were studied. When added separately, IL-1-alpha was able to support the growth ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1987.tb02263.x
更新日期：1987-09-01 00:00:00
abstract：:Pretreatment with D-Penicillamine of rat peritoneal macrophages markedly influences their ability to modulate Concanavalin A stimulated [3H]thymidine incorporation in non-adherent rat lymph node cells. Untreated macrophages added in high numbers to the lymphocytes (ratio lymphocytes/macrophage 7.5 : 1) depress their [...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1978.tb00453.x
更新日期：1978-04-01 00:00:00
abstract：:Allogeneic chimaeras that utilize C.B-17 SCID mice (H-2d) as recipients of MHC mismatched bone marrow from C57B1/6 (H-2b) or SJL/J (H-2s) mice have been used to provide experimental evidence demonstrating the necessity for a direct interaction between an effector T helper and the cytotoxic T-cell precursor in order to...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1992.tb03262.x
更新日期：1992-05-01 00:00:00
abstract：:Jacalin, a D-galactose-specific lectin from jackfruit, interacts with human IgA and one or two other serum proteins. Incubation of jacalin with fresh human serum was shown to result in activation of the complement system. Therefore the mechanism of complement activation by jacalin was studied. Jacalin was extracted fr...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1987.tb02243.x
更新日期：1987-08-01 00:00:00
abstract：:Crude preparations of platelet-activating factors (PAF) derived by methanolic extraction of supernatants from antigen-challenged basophils generally stimulated the secretion of histamine from human peripheral blood leucocytes when co-incubated with these cells in the absence of an additional stimulator. When these cru...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1981.tb00168.x
更新日期：1981-01-01 00:00:00
abstract：:The association of HLA-A and -B antigens with Bf alleles was investigated in 200 parents from 100 unrelated families. There were significant associations between HLA-A3 and Bf-F, B7 and Bf-S, B8 and Bf-S, B12 and Bf-F, and BW 35 and Bf-F. Three-point HLA-A,B,Bf haplotype frequencies, linkage disequilibrium parameters,...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1977.tb02103.x
更新日期：1977-01-01 00:00:00
abstract：:Interferon gamma (IFN-gamma) production as a measure of cellular sensitization was studied by detection of the cytokine in culture supernatant by enzyme immunoassay (EIA) and by measuring cellular mRNA using the reverse transcriptase polymerase chain reaction (RT-PCR) method. These assays were compared to the standard...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.1997.d01-144.x
更新日期：1997-10-01 00:00:00
abstract：:In a well-established murine abortion model, stress is thought to trigger fetal rejection by inducing a proinflammatory immune response via substance P (SP), being tumour necrosis factor (TNF)-alpha-producing CD8+ T cells involved. Interestingly, the SP metabolite SP5-11 also binds to SP receptors and mediates SP-like...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2005.001711.x
更新日期：2006-01-01 00:00:00
abstract：:An antiserum to unstimulated rat peritoneal macrophages was produced in rabbits. The antibodies were directed against an acidic protein with a molecular weight of 35,000 and with an isoelectric point at 4.6. The macrophage acidic protein (MAP) was purified by gel filtration of rat lung soluble proteins, followed by pr...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1981.tb00576.x
更新日期：1981-10-01 00:00:00
abstract：:The T-cell differentiation antigen T4/Leu3 has been described as a non-polymorphic molecule important in T-cell recognition of class II major histocompatibility complex antigens. We report the polymorphism of this molecule in black individuals as manifest by a heterogeneity of staining with OKT4 monoclonal antibody. N...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1984.tb01003.x
更新日期：1984-09-01 00:00:00
abstract：:Discontinuous Percoll gradients have been used to obtain selected human peripheral blood T lymphocytes without having to resort to interactions with immune complexes in the fractionation of Tm, Tg and Ta cells. Here, we could show that Ta cells represent a heterogeneous population with no distinct density profile, in ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1982.tb00719.x
更新日期：1982-09-01 00:00:00
abstract：:Using ELISA, we have quantified the levels of IL-2 and IFN-gamma in the oral mucosa, ear skin and regional and distant lymph nodes in an experimental murine model of contact sensitivity (CS), induced by the hapten oxazolone (OXA). Compared to normal conditions, the levels of IL-2 peaked early (4-6 h) after hapten expo...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2010.02402.x
更新日期：2010-07-01 00:00:00
abstract：:Chronic obstructive pulmonary disease (COPD) is a common respiratory condition involving the airways and characterized by airflow limitation. Gaseous and noxious particles play an important role in this process. Antioxidants are the substances that may protect cells from the damage caused by unstable molecules known a...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/sji.12498
更新日期：2017-02-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1981.tb00154.x
更新日期：1981-01-01 00:00:00
abstract：:Screening for B-cell-specific antibodies in unabsorbed pregnancy sera preselected for weak reactivity in regular HLA-A, -B, and -C screening yielded a relatively high proportion (35/81) of B-cell-specific antibodies. Most B-cell-specific antibodies react broadly, showing inclusion phenomena suggesting 'cross-reactivit...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1977.tb02099.x
更新日期：1977-01-01 00:00:00
abstract：:World Health Organisation recommends the practice of BCG vaccination at birth in countries which have a high incidence of tuberculosis and/or high leprosy burden. The BCG vaccination is considered safe for a competent immune system. However, in children with weakened immune systems cause of which can be primary or sec...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/sji.13010
更新日期：2020-12-16 00:00:00
abstract：:The light (L) and heavy (H) chain and the idiotypic (Id) composition of the antibody (Ab) plaque-forming cells (PFC) and serum Ab specific for alpha-1,3 dextran have been characterized in murine strains exhibiting the CH-Ig-lb to e allotypes and in their F1 hybrids with Ig-la1, BALB/c prototypes. The Ab response of th...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1979.tb02724.x
更新日期：1979-03-01 00:00:00
abstract：:Epithelial expression of class II antigens encoded by the major histocompatibility complex (MHC) has been proposed as a means by which autoimmune thyroid disease may be initiated and maintained. We studied a rat thyroid epithelial cell line (FRTL-5), which constitutively expresses class I (OX18) but not class II (OX6 ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1987.tb01088.x
更新日期：1987-06-01 00:00:00
abstract：:Gangliosides are complex glycosphingolipids, which exert immune-modulating effects on various cell types. Ganglioside GD(3) and GM(3) are the predominant gangliosides of human breast milk but during the early phase of lactation, the content of GD(3) decreases while GM(3) increases. The biological value of gangliosides...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2005.01566.x
更新日期：2005-06-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1994.tb03479.x
更新日期：1994-10-01 00:00:00
abstract：:Recently, associations were found between several autoimmune diseases and functional variants of interleukin-23 receptor (IL23R) gene; here, we studied the possible association of nine polymorphisms of IL23R with ankylosing spondylitis (AS) and with Sjögren syndrome (SS). In our study, we genotyped groups of patients ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2009.02265.x
更新日期：2009-07-01 00:00:00
abstract：:The binding of human serum amyloid P component (SAP) to blood cells and monocyte-derived dendritic cells was investigated by flow cytometry. Monocytes bound biotinylated SAP with avidity in a dose-dependent and saturable manner. By contrast, the binding of SAP to monocyte-derived dendritic cells was weak. No binding t...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2006.01774.x
更新日期：2006-07-01 00:00:00
abstract：:Purified human amyloid protein A (AA) or serum amyloid protein A (SAA) was incubated with normal human high-density lipoprotein (HDL). After ultracentrifugation the amount of AA or SAA associated with HDL was measured. It was found that the binding capacity of HDL for SAA was higher than that for AA. Incubation of the...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1988.tb01498.x
更新日期：1988-12-01 00:00:00
abstract：:The production of antibody by human colostral cells was assayed by the hemolysis in-gel technique. When sheep erythrocytes coated with O antigens from frequently encountered Escherichia coli bacteria were used as detector cells and anti-IgA serum was added for development, numerous plaque-forming cells (PFC) were demo...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1975.tb02659.x
更新日期：1975-09-01 00:00:00
abstract：:Cellular immune response and delayed-type hypersensitivity reactions are considered to play a major role in the immunopathogenesis of pulmonary tuberculosis (PTB). But the exact mechanism is still to be clarified. Th1 cells are mainly involved in cellular immune responses in PTB and provide a normal healing process wi...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2009.02294.x
更新日期：2009-09-01 00:00:00
abstract：:We investigated interferon (IFN) production by peripheral blood mononuclear cells from four patients with chronic OKT4 T-lymphocytic leukaemia and three patients with abnormal expansions of granular lymphocytes. No spontaneous production of IFN-gamma was found in supernatants of cultures from both patients and normal ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1985.tb01436.x
更新日期：1985-04-01 00:00:00
abstract：:The avidity of breast milk IgA antibody was studied with the aid of thiocyanate elution of antibody from solid-phase bound E.coli polysaccharides and diphtheria toxoid. The relative avidity index for each sample was determined by the molarity of thiocyanate required to elute 50% of the bound IgA antibody under conditi...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1988.tb01512.x
更新日期：1988-12-01 00:00:00
abstract：:We have previously shown that various benign and malignant natural killer (NK)-resistant monolayer cells inhibit endogenous human NK activity, probably by reducing the secretion of cytotoxic factors from the effector cells. The nature of the molecules responsible for the inhibition has been unclear. In this study we s...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1988.tb02411.x
更新日期：1988-07-01 00:00:00
abstract：:Expression of CD4+ or CD8+ determinants and ten different TCR V beta genes was analysed by two-colour flow cytometry in lymph node cells (LNC) of BALB/c and dm2 (a BALB/c Ld loss mutant) mice. TCR V beta 14 expression of CD8+ T cells and TCR V beta 7 and 14 of CD4+ T Cells were significantly more frequent in BALB/c th...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1992.tb03230.x
更新日期：1992-10-01 00:00:00
abstract：:The ability of macrophage subpopulations, separated by virtue of differences in size, to liberate soluble factors able to reconstitute antibody and cell-mediated immune responses in macrophage-depleted lymphoid cultures has been investigated. Evidence is presented to show that all macrophages release trypsin- and peps...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1977.tb02148.x
更新日期：1977-01-01 00:00:00
