Abstract：
:We have followed the migration of GFP-labelled nuclei in multinucleate hyphae of Ashbya gossypii. For the first time we could demonstrate that the mode of long range nuclear migration consists of oscillatory movements of nuclei with, on average, higher amplitudes in the direction of the growing tip. We could also show that mitotic division proceeds at a constant rate of 0. 64 microm/minute which differs from the biphasic kinetics described for the yeast Saccharomyces cerevisiae. Furthermore we were able to identify the microtubule-based motor dynein as a key element in the control of long range nuclear migration. For other filamentous fungi it had already been demonstrated that inactivating mutations in dynein led to severe problems in nuclear migration, i.e. generation of long nuclei-free hyphal tips and clusters of nuclei throughout the hyphae. This phenotype supported the view that dynein is important for the movement of nuclei towards the tip. In A. gossypii the opposite seems to be the case. A complete deletion of the dynein heavy chain gene leads to nuclear clusters exclusively at the hyphal tips and to an essentially nucleus-free network of hyphal tubes and branches. Anucleate hyphae and branches in the vicinity of nuclear clusters show actin cables and polarized actin patches, as well as microtubules. The slow growth of this dynein null mutant could be completely reverted to wild-type-like growth in the presence of benomyl, which can be explained by the observed redistribution of nuclei in the hyphal network.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Alberti-Segui C,Dietrich F,Altmann-Jöhl R,Hoepfner D,Philippsen Pkeywords:
subject
Has Abstractpub_date
2001-03-01 00:00:00pages
975-86issue
Pt 5eissn
0021-9533issn
1477-9137journal_volume
114pub_type
杂志文章abstract：:Immediately following embryonic cleavage, the cells of Astrephomene have four equal-sized basal bodies, two of which are connected by a striated distal fibre and two striated proximal fibres. The four microtubular rootlets, which alternate between having 3/1 and 2 members, are arranged cruciately. The two basal bodies...
journal_title：Journal of cell science
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journal_title：Journal of cell science
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更新日期：2002-11-15 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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更新日期：1998-08-01 00:00:00
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更新日期：1998-07-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2000-11-01 00:00:00
