当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Ca2+-independent and Ca2+/GTP-binding protein-controlled exocytosis in a plant cell.

Ca2+-independent and Ca2+/GTP-binding protein-controlled exocytosis in a plant cell.

Abstract：

:Exocytosis allows the release of secretory products and the delivery of new membrane material to the plasma membrane. So far, little is known about the underlying molecular mechanism and its control in plant cells. We have used the whole-cell patch-clamp technique to monitor changes in membrane capacitance to study exocytosis in barley aleurone protoplasts. To investigate the involvement of Ca2+ and GTP-binding proteins in exocytosis, protoplasts were dialyzed with very low (<2 nM) and high (1 microM) free Ca2+ and nonhydrolyzable guanine nucleotides guanosine 5'-gamma-thio]triphosphate (GTP[gammaS]) or guanosine 5'-[beta-thio]diphosphate (GDP[betaS]). With less than 2 nM cytoplasmic free Ca2+, the membrane capacitance increased significantly over 20 min. This increase was not altered by GTP[gammaS] or GDP[betaS]. In contrast, dialyzing protoplasts with 1 microM free Ca2+ resulted in a large increase in membrane capacitance that was slightly reduced by GTP[gammaS] and strongly inhibited by GDP[betaS]. We conclude that two exocytotic pathways exist in barley aleurone protoplasts: one that is Ca2+-independent and whose regulation is currently not known and another that is stimulated by Ca2+ and modulated by GTP-binding proteins. We suggest that Ca2+-independent exocytosis may be involved in cell expansion in developing protoplasts. Ca2+-stimulated exocytosis may play a role in gibberellic acid-stimulated alpha-amylase secretion in barley aleurone and, more generally, may be involved in membrane resealing in response to cell damage.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Homann U,Tester M

doi

10.1073/pnas.94.12.6565

keywords:

subject

Has Abstract

pub_date

1997-06-10 00:00:00

pages

6565-70

issue

12

eissn

0027-8424

issn

1091-6490

journal_volume

94

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Profound differences in potassium current properties of normal and Rous sarcoma virus-transformed chicken embryo fibroblasts.

    abstract：:The membrane currents of chicken embryo fibroblasts (CEFs) transformed by Rous sarcoma virus (RSV) were compared with the currents of their nontransformed counterparts by using the whole-cell patch-clamp technique. In nontransformed CEFs, the main membrane current is a delayed outward K+ current that is sensitive to t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.8.3403

    authors： Repp H,Draheim H,Ruland J,Seidel G,Beise J,Presek P,Dreyer F

    更新日期：1993-04-15 00:00:00

  • Inhibition of pancreatic carcinoma by homo- and heterocombinations of antibodies against EGF-receptor and its kin HER2/ErbB-2.

    abstract：:Due to intrinsic aggressiveness and lack of effective therapies, prognosis of pancreatic cancer remains dismal. Because the only molecular targeted drug approved for pancreatic ductal adenocarcinoma is a kinase inhibitor specific to the epidermal growth factor receptor (EGFR), and this receptor collaborates with anoth...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1313857110

    authors： Maron R,Schechter B,Mancini M,Mahlknecht G,Yarden Y,Sela M

    更新日期：2013-09-17 00:00:00

  • New evidence on the impact of sustained exposure to air pollution on life expectancy from China's Huai River Policy.

    abstract：:This paper finds that a 10-μg/m3 increase in airborne particulate matter [particulate matter smaller than 10 μm (PM10)] reduces life expectancy by 0.64 years (95% confidence interval = 0.21-1.07). This estimate is derived from quasiexperimental variation in PM10 generated by China's Huai River Policy, which provides f...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1616784114

    authors： Ebenstein A,Fan M,Greenstone M,He G,Zhou M

    更新日期：2017-09-26 00:00:00

  • Lipophosphoglycan is a virulence factor distinct from related glycoconjugates in the protozoan parasite Leishmania major.

    abstract：:Protozoan parasites of the genus Leishmania undergo a complex life cycle involving transmission by biting sand flies and replication within mammalian macrophage phagolysosomes. A major component of the Leishmania surface coat is the glycosylphosphatidylinositol (GPI)-anchored polysaccharide called lipophosphoglycan (L...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.160257897

    authors： Späth GF,Epstein L,Leader B,Singer SM,Avila HA,Turco SJ,Beverley SM

    更新日期：2000-08-01 00:00:00

  • In vitro analysis of DNA-protein interactions by proximity ligation.

    abstract：:Protein-binding DNA sequence elements encode a variety of regulated functions of genomes. Information about such elements is currently in a state of rapid growth, but improved methods are required to characterize the sequence specificity of DNA-binding proteins. We have established an in vitro method for specific and ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0611229104

    authors： Gustafsdottir SM,Schlingemann J,Rada-Iglesias A,Schallmeiner E,Kamali-Moghaddam M,Wadelius C,Landegren U

    更新日期：2007-02-27 00:00:00

  • Reentrant spiral waves of spreading depression cause macular degeneration in hypoglycemic chicken retina.

    abstract：:Spreading depression (SD), a slow diffusion-mediated self-sustained wave of depolarization that severely disrupts neuronal function, has been implicated as a cause of cellular injury in a number of central nervous system pathologies, including blind spots in the retina. Here we show that in the hypoglycemic chicken re...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1121111109

    authors： Yu Y,Santos LM,Mattiace LA,Costa ML,Ferreira LC,Benabou K,Kim AH,Abrahams J,Bennett MV,Rozental R

    更新日期：2012-02-14 00:00:00

  • Peroxisomal D-hydroxyacyl-CoA dehydrogenase deficiency: resolution of the enzyme defect and its molecular basis in bifunctional protein deficiency.

    abstract：:Peroxisomes play an essential role in a number of different metabolic pathways, including the beta-oxidation of a distinct set of fatty acids and fatty acid derivatives. The importance of the peroxisomal beta-oxidation system in humans is made apparent by the existence of a group of inherited diseases in which peroxis...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.5.2128

    authors： van Grunsven EG,van Berkel E,Ijlst L,Vreken P,de Klerk JB,Adamski J,Lemonde H,Clayton PT,Cuebas DA,Wanders RJ

    更新日期：1998-03-03 00:00:00

  • The evolution of coloration and toxicity in the poison frog family (Dendrobatidae).

    abstract：:The poison frogs (family Dendrobatidae) are terrestrial anuran amphibians displaying a wide range of coloration and toxicity. These frogs generally have been considered to be aposematic, but relatively little research has been carried out to test the predictions of this hypothesis. Here we use a comparative approach t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.101134898

    authors： Summers K,Clough ME

    更新日期：2001-05-22 00:00:00

  • Phenotypic plasticity facilitates recurrent rapid adaptation to introduced predators.

    abstract：:A central role for phenotypic plasticity in adaptive evolution is often posited yet lacks empirical support. Selection for the stable production of an induced phenotype is hypothesized to modify the regulation of preexisting developmental pathways, producing rapid adaptive change. We examined the role of plasticity in...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0912748107

    authors： Scoville AG,Pfrender ME

    更新日期：2010-03-02 00:00:00

  • Conformational changes induced in dihydrofolate reductase by folates, pyridine nucleotide coenzymes, and methotrexate.

    abstract：:Dihydrofolate reductase (EC 1.5.1.3; 5,6,7,8-tetrahydrofolate:NADP(+) oxidoreductase) from antifolate-resistant Lactobacillus casei has been isolated in pure form and examined in solution by high resolution proton magnetic resonance spectroscopy. The 220 MHz proton magnetic resonance spectrum of this small enzyme (abo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.10.3849

    authors： Pastore EJ,Kisliuk RL,Plante LT,Wright JM,Kaplan NO

    更新日期：1974-10-01 00:00:00

  • Arc/Arg3.1 mediates a critical period for spatial learning and hippocampal networks.

    abstract：:During early postnatal development, sensory regions of the brain undergo periods of heightened plasticity which sculpt neural networks and lay the foundation for adult sensory perception. Such critical periods were also postulated for learning and memory but remain elusive and poorly understood. Here, we present evide...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1810125115

    authors： Gao X,Castro-Gomez S,Grendel J,Graf S,Süsens U,Binkle L,Mensching D,Isbrandt D,Kuhl D,Ohana O

    更新日期：2018-12-04 00:00:00

  • Distribution of UDPglucuronosyltransferase in rat tissue.

    abstract：:UDPglucuronosyltransferase [UDPglucuronate beta-D-glucuronosyltransferase (acceptor-unspecific), EC 2.4.1.17] is a group of enzymes with distinct but partially overlapping substrate specificity. A rabbit antiserum raised against one purified rat liver UDPglycuronosyltransferase isoform was specific for UDPglucuronosyl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.9.2990

    authors： Chowdhury JR,Novikoff PM,Chowdhury NR,Novikoff AB

    更新日期：1985-05-01 00:00:00

  • Contrasting nitrogen and phosphorus budgets in urban watersheds and implications for managing urban water pollution.

    abstract：:Managing excess nutrients remains a major obstacle to improving ecosystem service benefits of urban waters. To inform more ecologically based landscape nutrient management, we compared watershed inputs, outputs, and retention for nitrogen (N) and phosphorus (P) in seven subwatersheds of the Mississippi River in St. Pa...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1618536114

    authors： Hobbie SE,Finlay JC,Janke BD,Nidzgorski DA,Millet DB,Baker LA

    更新日期：2017-04-18 00:00:00

  • Swainsonine: an inhibitor of glycoprotein processing.

    abstract：:Swainsonine, an indolizidine alkaloid, inhibits the processing of asparagine-linked glycoproteins in both cell-free extracts and animal cells in culture. Thus, in a liver particulate enzyme preparation, swainsonine at 0.1-1.0 microM inhibited the mannosidase that releases [3H]mannose from a high mannose glycopeptide b...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.12.7393

    authors： Elbein AD,Solf R,Dorling PR,Vosbeck K

    更新日期：1981-12-01 00:00:00

  • Long-term neurocognitive benefits of FLASH radiotherapy driven by reduced reactive oxygen species.

    abstract：:Here, we highlight the potential translational benefits of delivering FLASH radiotherapy using ultra-high dose rates (>100 Gy⋅s-1). Compared with conventional dose-rate (CONV; 0.07-0.1 Gy⋅s-1) modalities, we showed that FLASH did not cause radiation-induced deficits in learning and memory in mice. Moreover, 6 months a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1901777116

    authors： Montay-Gruel P,Acharya MM,Petersson K,Alikhani L,Yakkala C,Allen BD,Ollivier J,Petit B,Jorge PG,Syage AR,Nguyen TA,Baddour AAD,Lu C,Singh P,Moeckli R,Bochud F,Germond JF,Froidevaux P,Bailat C,Bourhis J,Vozenin MC

    更新日期：2019-05-28 00:00:00

  • Augmentor α and β (FAM150) are ligands of the receptor tyrosine kinases ALK and LTK: Hierarchy and specificity of ligand-receptor interactions.

    abstract：:Receptor tyrosine kinases (RTKs) are a class of cell surface receptors that, upon ligand binding, stimulate a variety of critical cellular functions. The orphan receptor anaplastic lymphoma kinase (ALK) is one of very few RTKs that remain without a firmly established protein ligand. Here we present a novel cytokine, F...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1520099112

    authors： Reshetnyak AV,Murray PB,Shi X,Mo ES,Mohanty J,Tome F,Bai H,Gunel M,Lax I,Schlessinger J

    更新日期：2015-12-29 00:00:00

  • Bile acid stimulates hepatocyte polarization through a cAMP-Epac-MEK-LKB1-AMPK pathway.

    abstract：:This study describes a unique function of taurocholate in bile canalicular formation involving signaling through a cAMP-Epac-MEK-Rap1-LKB1-AMPK pathway. In rat hepatocyte sandwich cultures, polarization was manifested by sequential progression of bile canaliculi from small structures to a fully branched network. Tauro...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1018376108

    authors： Fu D,Wakabayashi Y,Lippincott-Schwartz J,Arias IM

    更新日期：2011-01-25 00:00:00

  • Photoregulation of biological activity by photocromic reagents. II. Inhibitors of acetylcholinesterase.

    abstract：:The enzymic activity of acetylcholinesterase can be photoregulated through the mediation of photochromic inhibitors of the enzyme. N-p-phenylazophenyl-N-phenylcarbamyl fluoride, an irreversible inhibitor of acetylcholinesterase, exists as two geometric isomers which are interconvertible through the action of light. Th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.64.3.1103

    authors： Bieth J,Vratsanos SM,Wassermann N,Erlanger BF

    更新日期：1969-11-01 00:00:00

  • Na3[B20H17NH3]: synthesis and liposomal delivery to murine tumors.

    abstract：:The polyhedral borane ion [n-B20H18]2- reacts with liquid ammonia in the presence of a suitable base to produce an apical-equatorial (ae) isomer of the [B20H17NH3]3- ion, [1-(2'-B10H9)-2-NH3B10H8]3-. The structure of this product has been confirmed by 11B NMR spectroscopy and x-ray crystallography. This species underg...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.8.3029

    authors： Feakes DA,Shelly K,Knobler CB,Hawthorne MF

    更新日期：1994-04-12 00:00:00

  • The human Thy-1 gene: structure and chromosomal location.

    abstract：:The human Thy-1 gene has been isolated and sequenced and compared to the rat and mouse Thy-1 genes. All three genes are organized in the same way: one exon encoding the majority of the signal peptide, another encoding the transmembrane segment, and a third encoding the remainder of the protein. One major structural di...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.19.6657

    authors： Seki T,Spurr N,Obata F,Goyert S,Goodfellow P,Silver J

    更新日期：1985-10-01 00:00:00

  • The ubiquitin ligase Siah2 regulates tumorigenesis and metastasis by HIF-dependent and -independent pathways.

    abstract：:The ubiquitin ligase Siah2 has been shown to regulate prolyl hydroxylase 3 (PHD3) stability with concomitant effect on HIF-1alpha availability. Because HIF-1alpha is implicated in tumorigenesis and metastasis, we used SW1 mouse melanoma cells, which develop primary tumors with a propensity to metastasize, in a syngene...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0804063105

    authors： Qi J,Nakayama K,Gaitonde S,Goydos JS,Krajewski S,Eroshkin A,Bar-Sagi D,Bowtell D,Ronai Z

    更新日期：2008-10-28 00:00:00

  • Radiolabeled somatostatin receptor antagonists are preferable to agonists for in vivo peptide receptor targeting of tumors.

    abstract：:Targeting neuroendocrine tumors expressing somatostatin receptor subtypes (sst) with radiolabeled somatostatin agonists is an established diagnostic and therapeutic approach in oncology. While agonists readily internalize into tumor cells, permitting accumulation of radioactivity, radiolabeled antagonists do not, and ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0607761103

    authors： Ginj M,Zhang H,Waser B,Cescato R,Wild D,Wang X,Erchegyi J,Rivier J,Mäcke HR,Reubi JC

    更新日期：2006-10-31 00:00:00

  • Origin of metazoan cadherin diversity and the antiquity of the classical cadherin/β-catenin complex.

    abstract：:The evolution of cadherins, which are essential for metazoan multicellularity and restricted to metazoans and their closest relatives, has special relevance for understanding metazoan origins. To reconstruct the ancestry and evolution of cadherin gene families, we analyzed the genomes of the choanoflagellate Salpingoe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1120685109

    authors： Nichols SA,Roberts BW,Richter DJ,Fairclough SR,King N

    更新日期：2012-08-07 00:00:00

  • Chimeric tumor necrosis factor-TrkA receptors reveal that ligand-dependent activation of the TrkA tyrosine kinase is sufficient for differentiation and survival of PC12 cells.

    abstract：:To elucidate the function of the two nerve growth factor (NGF) receptors, p75NGFR and p140trk, chimeric molecules were constructed of tumor necrosis factor (TNF) and NGF receptors. Rat PC12 pheochromocytoma cells transiently transfected with TNF-p140trk chimeras, which contain the extracellular domain of TNF receptor ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.18.8717

    authors： Rovelli G,Heller RA,Canossa M,Shooter EM

    更新日期：1993-09-15 00:00:00

  • Catalytic domain of human immunodeficiency virus type 1 integrase: identification of a soluble mutant by systematic replacement of hydrophobic residues.

    abstract：:The integrase protein of human immunodeficiency virus type 1 is necessary for the stable integration of the viral genome into host DNA. Integrase catalyzes the 3' processing of the linear viral DNA and the subsequent DNA strand transfer reaction that inserts the viral DNA ends into host DNA. Although full-length integ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.13.6057

    authors： Jenkins TM,Hickman AB,Dyda F,Ghirlando R,Davies DR,Craigie R

    更新日期：1995-06-20 00:00:00

  • The displacement field associated with the freezing of a melt and its role in determining crystal growth kinetics.

    abstract：:The atomic displacements associated with the freezing of metals and salts are calculated by treating crystal growth as an assignment problem through the use of an optimal transport algorithm. Converting these displacements into timescales based on the dynamics of the bulk liquid, we show that we can predict the activa...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1915806117

    authors： Sun G,Hawken A,Harrowell P

    更新日期：2020-02-18 00:00:00

  • Muscarinic and beta-adrenergic depression of the slow Ca2(+)-activated potassium conductance in hippocampal CA3 pyramidal cells is not mediated by a reduction of depolarization-induced cytosolic Ca2+ transients.

    abstract：:Combined intracellular and microfluorometric recording techniques were used to evaluate whether the inhibition by cholinergic or adrenergic transmitters of the Ca2(+)-activated potassium current (IAHP) in hippocampal CA3 pyramidal cells was mediated by an alteration of depolarization-induced change in cytosolic free C...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.11.4083

    authors： Knöpfel T,Vranesic I,Gähwiler BH,Brown DA

    更新日期：1990-06-01 00:00:00

  • Stereospecific gating of functional motions in Pin1.

    abstract：:Pin1 is a modular enzyme that accelerates the cis-trans isomerization of phosphorylated-Ser/Thr-Pro (pS/T-P) motifs found in numerous signaling proteins regulating cell growth and neuronal survival. We have used NMR to investigate the interaction of Pin1 with three related ligands that include a pS-P substrate peptide...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1019382108

    authors： Namanja AT,Wang XJ,Xu B,Mercedes-Camacho AY,Wilson KA,Etzkorn FA,Peng JW

    更新日期：2011-07-26 00:00:00

  • Evolved tooth gain in sticklebacks is associated with a cis-regulatory allele of Bmp6.

    abstract：:Developmental genetic studies of evolved differences in morphology have led to the hypothesis that cis-regulatory changes often underlie morphological evolution. However, because most of these studies focus on evolved loss of traits, the genetic architecture and possible association with cis-regulatory changes of gain...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1407567111

    authors： Cleves PA,Ellis NA,Jimenez MT,Nunez SM,Schluter D,Kingsley DM,Miller CT

    更新日期：2014-09-23 00:00:00

  • CCAAT/enhancer binding protein alpha (C/EBP alpha) undifferentiated protein: a developmentally regulated nuclear protein that binds to the C/EBP alpha gene promoter.

    abstract：:During differentiation of 3T3-L1 preadipocytes into adipocytes, transcription of the C/EBP alpha (CCA-AT/enhancer binding protein alpha) gene is activated. The promoter of the C/EBP alpha gene contains a bipartite cis element with binding sites for C/EBP alpha undifferentiated protein (CUP) and an Sp1-like GT box bind...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.15.7312

    authors： Vasseur-Cognet M,Lane MD

    更新日期：1993-08-01 00:00:00