Sustained GABA-induced regulation of the inactivation of the voltage-dependent Ca2+ current in crustacean muscle fibers.

Abstract:

:We describe a new form of gamma-aminobutyric acid (GABA) -mediated regulation of the inactivation and of the recovery from inactivation of the L-type Ca2+ current (I(Ca)) in crayfish muscle. GABA (1 mM) was applied during a 2-min period and the peak I(Ca) was measured using two-electrode voltage-clamp recordings 30 min after returning to the control solution. Prepulse-pulse protocols showed that the GABA-mediated inhibition of I(Ca) decreased (>50%) both with increasing prepulse depolarization and as the delay between prepulse and pulse was reduced. GABA also shifted to depolarized values (>5 mV) the S-shaped plots of the peak I(Ca) evoked by a constant depolarizing pulse as a function of prepulse voltage (i.e., inactivation curves) and accelerated the recovery time from the inactivation evoked by depolarizing prepulses (>35%). The effects outlasted GABA application up to 1 h. The observed changes in inactivation properties may be of functional importance because they indicate that previous depolarization relieves the GABA-induced inhibition of I(Ca), implying that this long-lasting inhibition is under the regulation of the prepulse potential and the subsequent Ca2+ entry.

journal_name

Exp Brain Res

authors

Castellote JM,Buño W

doi

10.1007/s002210000445

keywords:

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

90-5

issue

1

eissn

0014-4819

issn

1432-1106

journal_volume

134

pub_type

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