Abstract:
:Considerable evidence suggests that low (picomolar) concentrations of the HIV-1 envelope glycoprotein gp120 induce neuronal cell death by stimulating the release of microglial toxins, which in turn activate N-methyl-D-aspartate (NMDA) receptors. Conversely, high (micromolar) concentrations of gp120 have been reported to directly inhibit NMDA receptor-mediated currents and do not induce neurotoxicity. Here we show that micromolar concentrations of a synthetic peptide corresponding to the V3-loop of gp120 (V3-pep) inhibited agonist responses of recombinant heteromeric rodent NMDA receptors expressed in Xenopus laevis oocytes by decreasing their apparent glycine affinity. Different combinations of NMDA receptor subunits displayed differential sensitivities to inhibition by V3-pep, with a potency rank order of NR1/2B > NR1/2D > NR1/2C > or = NR1/2A. Our observations may provide an explanation for the reduced neurotoxicity of high doses of gp120 in cell cultures and may be useful for the pharmacological discrimination of NMDA receptor subtypes.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Wittekindt B,Betz H,Laube Bdoi
10.1016/s0304-3940(00)00775-8keywords:
subject
Has Abstractpub_date
2000-02-18 00:00:00pages
151-4issue
2eissn
0304-3940issn
1872-7972pii
S0304-3940(00)00775-8journal_volume
280pub_type
杂志文章abstract::Intracellular regulation of NMDA-receptor-mediated currents in cultured rat spinal dorsal horn neurons was investigated by means of simultaneously recording whole-cell currents and intracellular Ca2+ concentration ([Ca2+]i). During recordings in which EGTA (11 mM) was used to buffer intracellular Ca2+, NMDA currents s...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(93)90732-z
更新日期:1993-07-23 00:00:00
abstract::To investigate whether there is a descending contribution to the spinal 5-hydroxytryptamine (5-HT) innervation in the lamprey, a primitive vertebrate, a study using retrograde transport of the fluorescent tracer Fast blue combined with 5-HT immunohistochemistry was conducted. Two to 4 weeks after an injection of Fast ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(86)90207-7
更新日期:1986-06-06 00:00:00
abstract::The effect of dexmedetomidine, a selective alpha2-adrenoreceptor agonist and neuroprotective agent on the expression of immediate early genes and heat shock protein hsp70, was studied using quantitative in situ hybridization in a global ischemia model. At the dose previously shown to be neuroprotective dexmedetomidine...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(97)00585-5
更新日期:1997-08-29 00:00:00
abstract::Neuropathy is observed in 50% of diabetic patients with diabetic foot. This study attempted to explore the potential role of human mesenchymal stem cells-umbilical cord blood (hMSCs-UC) in femoral nerve (FN) neuropathy. The model rats were established by one time administration of streptozotocin and empyrosis on the d...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2015.04.038
更新日期:2015-06-15 00:00:00
abstract::Involvement of 5-hydroxytryptaminergic (5-HT) system for aglycemia- and ischemia-induced depression of the spinal reflexes was evaluated. The monosynaptic (MSR) and polysynaptic (PSR) reflex potentials were elicited in the ventral root by stimulating the corresponding dorsal root in an isolated spinal cord from neonat...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2004.09.033
更新日期:2004-11-30 00:00:00
abstract::Neurons within the hypothalamic paraventricular nuclei (PVN) have been antidromically invaded following electrical stimulation within the pons and medulla of rats. These neurones are dispersed throughout the PVN and are often found near neurones antidromically invaded only from the pituitary salk. In addition, a small...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(81)90033-1
更新日期:1981-05-06 00:00:00
abstract::Although the therapeutic use of microglia has received some attention for the treatment of brain diseases, few non-invasive techniques exist for monitoring the cells after administration. Here, we present a technique using magnetic resonance imaging (MRI) to track microglia injected intra-cardially. We labeled microgl...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.10.074
更新日期:2006-02-27 00:00:00
abstract::The insular cortex has emerged as a novel target for nicotine addiction research. One unresolved question about the insular cortex is whether its neurons exhibit nicotine-induced dendritic remodeling similar to other brain regions implicated in nicotine addiction. To test this question, Long-Evans rats were administer...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2012.03.064
更新日期:2012-05-10 00:00:00
abstract::Conditioned environmental stimuli are known to be important determinants of drug seeking behavior. c-Fos, the protein product of the protooncogene c-Fos, is expressed in neurons when there are drug-associated cue-induced drug-seeking behaviour. Therefore, its expression could serve as a marker of regional neuronal act...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.06.008
更新日期:2005-09-30 00:00:00
abstract::We have previously reported that there is an up-regulation of the NR2B gene expression in the adult cortex and cultured fetal cortical neurons of mice following chronic ethanol treatment due to demethylation of cytosine residues in the NR2B gene CpG island. In the present study, we investigated the methylation pattern...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.01.042
更新日期:2005-06-03 00:00:00
abstract::Morphine and other opioids have direct analgesic actions in the spinal cord and chronic spinal administration of opioid agonists is used clinically in patients suffering from severe, chronic pain. Neuropathic pain resulting from peripheral nerve injury is often less sensitive to opioid therapy than other forms of chro...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2003.12.067
更新日期:2004-05-06 00:00:00
abstract::Pargyline, an inhibitor of monoamine oxidase (MAO), prevented 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced inhibition of dihydroxyphenylalanine (DOPA) production by tyrosine hydroxylase (TH) system in rat striatal tissue slices. The result suggests that the metabolism of MPTP in rat striatal tissue slic...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(85)90509-9
更新日期:1985-06-24 00:00:00
abstract::Using the Golgi silver impregnation technique the present study examines the morphology and development of presumptive local circuit neurons in lamina III of the rat lumbar spinal cord. These neurons generate local axonal plexuses which remain within the gray matter and dendritic trees which arborize in lamina III and...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(88)90172-3
更新日期:1988-03-21 00:00:00
abstract::Increasing evidence suggests a correlation between cytokine-induced nitric oxide synthase (iNOS) and demyelination in Multiple sclerosis (MS). Inhibition of iNOS may therefore be a novel therapeutic approach in MS. To test an antisense oligodeoxynucleotide (ODN) knockdown strategy for inhibiting iNOS, we used lipopoly...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(96)13229-8
更新日期:1996-12-13 00:00:00
abstract::Bax, a pro-apoptotic member of Bcl-2 family proteins, plays a central role in mitochondria-dependent apoptosis. Bax normally resides in the cytosol in a quiescent state. Bax-inhibiting peptide (BIP) is a membrane permeable peptide comprised of five amino acids designed from the Bax-binding domain of Ku70 [M. Sawada, P...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2004.08.075
更新日期:2004-11-30 00:00:00
abstract::Three dopamine receptor mRNAs were detected by reverse polymerase chain reaction in the rat forebrain during ontogeny. The D1 receptor mRNA gave a faint signal from gestational day 11 (G11) on, while the D1b receptor was not detectable before G12. The D3 receptor mRNA was slightly expressed at G11 and clearly present ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(93)90680-j
更新日期:1993-05-28 00:00:00
abstract::The effect of insulin-induced hypoglycemic stress on the concentrations of immunoreactive beta-endorphin (ir beta-EP) in plasma and cerebrospinal fluid (CSF) was examined in conscious non-pregnant ewes in which the cisterna magna and a jugular vein had been previously catheterized. In control experiments, no significa...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(84)90126-5
更新日期:1984-08-24 00:00:00
abstract::Direct projections from the A1/C1 catecholaminergic cell group in the caudal ventrolateral medulla oblongata to neurons containing vasopressin (VP) in the supraoptic nucleus (SON) were studied electron microscopically by a double-labeling technique which combines anterograde tracing after injection of wheat germ agglu...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90828-u
更新日期:1992-12-14 00:00:00
abstract::Noise-induced hearing loss (NIHL) has been thought to primarily involve damage to the sensory hair cells of the cochlea via mechanical and metabolic mechanisms. This study examined the effects of baicalin, baicalein, and Scutellaria baicalensis (SB) extract against NIHL in a mouse model. Mice received oral treatment w...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2009.12.009
更新日期:2010-01-29 00:00:00
abstract::The non-invasive imaging technique of diffusion tensor imaging (DTI) has been used to investigate the microstructural properties of white matter (WM). The present study investigated whether individual differences in the WM structure of normal subjects as measured by fractional anisotropy (FA) values correlate with cog...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2010.04.031
更新日期:2010-06-21 00:00:00
abstract::It is known that alcohol induces disorders in the metabolism of retinoids and particularly in the biosynthetic pathways of retinoic acid (RA). Since RA has, along with other hormones and particularly triiodothyronine (T3), a physiological role in the adult brain, the effect of chronic exposure to alcohol on RA and T3 ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(96)12455-1
更新日期:1996-03-15 00:00:00
abstract::The corticosterone (CORT) response to environmental perturbation has been shown to be enhanced by estrogen but inhibited by the androgen dihydrotestosterone (DHT). However, the mechanism of androgen's action has not been identified. This study examined the effects of estradiol benzoate (EB), the non-aromatizable andro...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2004.04.035
更新日期:2004-07-15 00:00:00
abstract::Nerve ligation injury in rats may represent a useful model of some clinical neuropathic pains. Activation of N-methyl-D-aspartate (NMDA) receptors may maintain central sensitivity and contribute to neuropathic pain. Here, nerve injury was produced by unilateral ligation of the L5 and L6 spinal roots of the sciatic ner...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(95)12022-v
更新日期:1995-10-20 00:00:00
abstract::The displaced retinal ganglion cells in normal rats and rats with left eye enucleated at birth were studied using the fluorescent dye Fast blue as neuronal marker. The dye was introduced to the right optic tract of the rats 7 days before perfusion. Frozen coronal sections of the brains and retinal whole-mounts from al...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(86)90315-0
更新日期:1986-06-30 00:00:00
abstract::In a light microscopic study the ferric ion-ferrocyanide staining of the squid giant axon was investigated. In other neurons this stain is known to indicate excitable membranes with high sodium channel density, such as the node of Ranvier or the initial segment. In the squid the stain reacted in small patches of the a...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(85)90055-2
更新日期:1985-06-12 00:00:00
abstract::The orexinergic neurons, localized in the perifornical hypothalamic area (PeF), are active during waking and quiet during non-rapid eye movement (non-REM) and REM sleep. Orexins promote arousal and suppress non-REM and REM sleep. Although in vitro studies suggest that PeF-orexinergic neurons are under glutamatergic in...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2008.05.042
更新日期:2008-07-18 00:00:00
abstract::Normal treatment of rats with monosodium-L-glutamate (MSG) resulted in extensive degeneration of the inner nuclear layer of the retina. The retino-hypothalamic projection was still functional as these rats entrained their circadian food intake rhythm to the light-dark cycle. A normal proportion of dorsal lateral genic...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(81)90407-9
更新日期:1981-09-25 00:00:00
abstract::This study used magnetoencephalography (MEG) to examine the dynamic patterns of neural activity underlying the auditory steady-state response. We examined the continuous time-series of responses to a 32-Hz amplitude modulation. Fluctuations in the amplitude of the evoked response were found to be mediated by non-linea...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.05.043
更新日期:2005-09-16 00:00:00
abstract::The retrofacial nucleus project bilaterally to the ventrolateral nucleus of the tractus solitarius (vlNTS) as revealed by means of retrograde transport of the fluorescent markers, Fast blue (FB) and Diamidino yellow (DY), in the cat. Some of the neurons of the retrofacial nucleus send axonal ramifications to both vlNT...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(87)90415-0
更新日期:1987-05-19 00:00:00
abstract::The rat spinal cord slice preparation has been used to investigate the sensitivity of deep dorsal horn neurones to the excitatory amino acids N-methyl-D-aspartate (NMDA), quisqualate and L-glutamate. Intracellular recordings were made from 44 neurones in laminae III-VI of 14- to 16-day rats. Superfusion of quisqualate...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(88)90541-1
更新日期:1988-07-08 00:00:00