Abstract:
:In the present study, we used co-culture of astrocytes from one species with neurons from a different species to examine neuritic outgrowth. We include a focus on human cells. Three types of neuron were used, including rat hippocampal dentate granule cells, rat hypothalamic neurons and human cortical neurons. To visualize neuronal processes, neurons were either immunostained with GABA antiserum or transfected with the jellyfish green fluorescent protein gene. The entire axonal and dendritic fields of single neurons could be quantitatively analysed based on their strong green fluorescent protein label. Astrocytes were obtained from rat hippocampus or hypothalamus, chicken cortex, normal human cortex, human cortex lesion, and from the sclerotic human hippocampus after surgery for intractable temporal lobe epilepsy. In the absence of astrocytes, isolated neurons died within three to four days. In contrast, neurons from both rat and human brains survived and extended dendrites and axons on rat, chicken and human astrocytes or in their conditioned medium. Astrocytes from interspecies cultures were not only capable of enhancing the survival of neuron co-cultures, but neuronal neurite extension in some cases was even greater on heterospecific astrocytes than on homospecific astrocytes. To support the hypothesis that synaptogenesis of rat hippocampal neurons was accelerated by a substrate of human astrocytes, we used a functional assay based on time-lapse confocal laser or digital imaging of calcium responses to transmitter release; synaptic responses were found earlier when rat neurons were grown on rat or human astrocytes than in the absence of these astrocytes. These data indicate that rodent glial cells enhance human neurite extension, and that rat neurite outgrowth can be used as a type of bioassay for the neurite promoting capacity of different derivations of human glia.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
van den Pol AN,Spencer DDdoi
10.1016/s0306-4522(99)00430-3keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
603-16issue
2eissn
0306-4522issn
1873-7544pii
S0306452299004303journal_volume
95pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::The unilateral application of GABA (10(-5) M; 30 min) into thalamic motor nuclei of the cat increases the release of dopamine in both caudate nuclei. This effect has been suggested to be related to an activation of the bilateral corticostriatal glutamatergic projection, glutamate exerting a presynaptic facilitatory in...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(89)90183-8
更新日期:1989-01-01 00:00:00
abstract::Far from disproving the model of mind functioning proposed by psychoanalysis, the recent advances in neuropsychiatrical research confirmed the crucial ideas of Sigmund Freud. The hypothesis that the origin of mental illnesses lies in the impossibility for a subject to erase the long-term effects of a remote adverse ev...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2004.09.032
更新日期:2005-01-01 00:00:00
abstract::We have previously demonstrated that progesterone significantly increases the rate of myelination in organotypic slice cultures of 7-day-old rat and mouse cerebellum. Here, we show that progesterone (20microM) stimulates the proliferation of oligodendrocyte precursors in cultured cerebellar slices of 7-day-old rats. T...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.05.023
更新日期:2005-01-01 00:00:00
abstract::The distribution of the urea cycle enzyme, argininosuccinate synthetase, in the rat brain was determined using immunohistochemistry. This enzyme participates in the only known metabolic pathway for citrulline, its condensation with aspartate to form argininosuccinate, which can then be cleaved to fumarate and arginine...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90519-8
更新日期:1992-12-01 00:00:00
abstract::Recent studies have shown a decrease in glial number and glial fibrillary acidic protein (GFAP) levels in the frontal and cingulate cortices of individuals with mood disorders and schizophrenia. In an attempt to verify and expand these findings we examined GFAP messenger ribonucleic acid (mRNA) levels in postmortem se...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.02.037
更新日期:2005-01-01 00:00:00
abstract::8-oxoguanine DNA glycosylase and Kin17 are proteins widely distributed and phylogenetically conserved in the CNS. 8-oxoguanine DNA glycosylase is a DNA repair enzyme that excises 7,8-dihydro-8-oxoguanine present in DNA damaged by oxidative stress. Kin17 protein is involved in DNA repair and illegitimate recombination ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.06.080
更新日期:2005-01-01 00:00:00
abstract::An antisense oligodeoxynucleotide selective for the rat neuropeptide Y1 receptor gene was given into the left lateral ventricle in the experimental group of rats, whereas a missense oligodeoxynucleotide or saline was given in the control groups. Some rats were decapitated at 1-2h after the last injection of the oligod...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00159-7
更新日期:2000-01-01 00:00:00
abstract::Amyloid-β (Aβ) production and clearance in the brain is a crucial focus of investigations into the pathogenesis of Alzheimer disease. Imbalance between production and clearance leads to accumulation of Aβ. The important Aβ-degrading enzymes in the brain are neprilysin (NEP) and insulin-degrading enzyme (IDE), and defe...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.08.030
更新日期:2017-10-24 00:00:00
abstract::Production of tissue inhibitors of matrix metalloproteinases (TIMPs), a family of secreted proteins with inhibitory actions on matrix metalloproteinases (MMPs), is up-regulated following nerve injuries and is suggested to have protective effects against MMP-mediated tissue damages. To clarify the extracellular signals...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.04.047
更新日期:2007-07-13 00:00:00
abstract::After transection of the sciatic nerve there is a dramatic increase in both galanin/galanin message-associated peptide-like immunoreactivities and preprogalanin messenger RNA levels in rat and mouse lumbar 4 and 5 dorsal root ganglion neurons. There is strong evidence that after nerve injury leukemia inhibitor factor ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00405-9
更新日期:1999-01-01 00:00:00
abstract::Ghrelin is a peptide produced in the gut with a wide range of physiological functions. Recent studies have suggested it may have potential as a neuroprotective agent in models of Parkinson's disease, reducing the impact of toxic challenges on the survival of nigral dopaminergic neurons. The presence of the ghrelin rec...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2018.10.045
更新日期:2018-12-15 00:00:00
abstract::The work of Aguayo and colleagues [Aguayo, David and Bray (1981) J. Exp. Biol. 95, 231-240] demonstrates that grafts of peripheral neural tissue are able to induce regenerative elongation of cut axons in the adult central nervous system. Elucidation of the mechanism of this response requires an understanding of the ce...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(87)90261-2
更新日期:1987-03-01 00:00:00
abstract::Effects of ionophoretic application of serotonin and of one of its agonists were tested on responses of dorsal horn dorsal spinocerebellar tract neurons evoked by electrical stimulation of peripheral nerves. Both drugs depressed monosynaptically evoked actions of group II muscle afferents; they decreased the number an...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00060-v
更新日期:1995-07-01 00:00:00
abstract::Selective replacement of degenerated neurons in the adult brain with allogeneic fetal neuroblasts is a promising therapeutic modality for human neurodegenerative diseases, but is confounded with practical and potential ethical problems. To evaluate the potential of xenogeneic donors as a cell source for neural transpl...
journal_title:Neuroscience
pub_type: 杂志文章,meta分析
doi:10.1016/0306-4522(94)90338-7
更新日期:1994-10-01 00:00:00
abstract::Differential vulnerability in the hindbrain neurons was examined immunohistochemically during hindbrain ischemia in the gerbil. Hindbrain ischemia was produced by extracranial occlusion of the bilateral vertebral arteries just before their entry into the transverse foramen of the cervical vertebra. Local cerebral bloo...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90343-e
更新日期:1993-09-01 00:00:00
abstract::The present study set out to assess the possible role of the medial prefrontal cortex (mPFC) cannabinoid CB1 receptors and BDNF/cFOS signaling pathways in morphine-dextromethorphan (DXM) cross state-dependent memory (SDM) using male Wistar rats. Changes on the levels of BDNF and cFOS proteins in the PFC were examined ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2018.10.012
更新日期:2018-11-21 00:00:00
abstract::Multiple system atrophy (MSA) is a sporadic neurodegenerative disease. The major pathological hallmark of MSA is the accumulation of α-synuclein in oligodendrocytes. In contrast to Parkinson's disease no definitive familial etiology for MSA has been determined. Yet, there is a growing body of evidence that perturbatio...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2016.02.042
更新日期:2016-05-13 00:00:00
abstract::Mammalian nociceptors have been classified into subclasses based on differential neurotrophin sensitivity and binding of the plant isolectin B4 (IB4). Most of the nerve growth factor-responsive IB4-negative (IB4 (-)) nociceptors contain neuropeptides such as substance P and calcitonin gene-related peptide, whereas the...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.09.053
更新日期:2007-01-19 00:00:00
abstract::Monoamine storage vesicles accumulate transmitters via an active transport process which presents similar pharmacological and bioenergetic properties in all monoaminergic systems. Using [3H]reserpine, a specific ligand of the vesicular monoamine transporter on isolated storage vesicles, we have determined the molecula...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(88)90207-2
更新日期:1988-12-01 00:00:00
abstract::A transient aberrant projection from the retina to the contralateral inferior colliculus was demonstrated in pigmented rats in both whole-brains and sections following intra-ocular injection of horseradish peroxidase. The projection was prominent on the day of birth but reached its maximum density and extent after inj...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(90)90087-k
更新日期:1990-01-01 00:00:00
abstract::The long-term effects of intrastriatal injections of the agonist of N-methyl-D-aspartate receptors, quinolinic acid, have been extensively characterized. Much less is known, however, about the early molecular and neurochemical changes which occur within a few hours of the toxin injection. In the present study, we have...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00239-0
更新日期:1999-01-01 00:00:00
abstract::Aging is associated with disturbances in autonomic cardiovascular control. The purpose of this study was to test the hypothesis that changes in nitric oxide occur with aging in brainstem nuclei involved in autonomic cardiovascular control. The principal and unexpected finding in this study was that NADPH-diaphorase re...
journal_title:Neuroscience
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::In synaptosomes prepared from dentate gyrus, activation of the metabotropic glutamate receptor by the specific agonist, trans-1-amino-cyclopentyl-1,3-dicarboxylate, increases release of glutamate in the presence of a low concentration of arachidonic acid. A similar interaction between trans-1-amino-cyclopentyl-1,3-dic...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00579-x
更新日期:1996-06-01 00:00:00
abstract::Neurons restore their function in response to external or internal perturbations and maintain neuronal or network stability through a homeostatic scaling mechanism. Homeostatic responses at synapses along the auditory system would be important for adaptation to normal and abnormal fluctuations in the sensory environme...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.07.049
更新日期:2009-11-10 00:00:00
abstract::Vitamin D is a neuroactive steroid. Its genomic actions are mediated via the active form of vitamin D, 1,25(OH)2D3, binding to the vitamin D receptor (VDR). The VDR emerges in the rat mesencephalon at embryonic day 12, representing the peak period of dopaminergic cell birth. Our prior studies reveal that developmental...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.07.048
更新日期:2015-09-24 00:00:00
abstract::The purpose of this study was to examine whether mechanisms, involved during the maintenance of familiar movement information in memory, were influenced by the degree of familiarity of the display in which the movements were embedded. Twelve gymnasts who possessed high visual and motor familiarity with the movements e...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.03.045
更新日期:2012-06-14 00:00:00
abstract::In the vestibular nuclei, GABAergic and glycinergic neurons play important roles in signal processing for normal function, during development, and after peripheral vestibular lesions. The chicken tangential nucleus is a major avian vestibular nucleus, whose principal cells are projection neurons with axons transmittin...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.11.050
更新日期:2011-02-23 00:00:00
abstract::Studies demonstrated that chronic high-dose homocysteine administration induced learning and memory impairment in animals. Atractylenolide III (Aen-III), a neuroprotective constituent of Atractylodis macrocephalae Koidz, was isolated in our previous study. In this study, we investigated potential benefits of Aen-III i...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.01.060
更新日期:2015-04-02 00:00:00
abstract::Rapid eye movement sleep deprivation increases Na-K ATPase activity and decreases aminergic neuronal firing rate as well as norepinephrine degrading enzyme, monoamine oxidase, activity. On the other hand, norepinephrine is known to increase Na-K ATPase activity. Hence, this study was conducted to find if the deprivati...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)00333-z
更新日期:1995-01-01 00:00:00
abstract::Rat cerebellar granule cells, when subjected to a glucose-free environment for 4 h, developed extensive degeneration of neuronal cell bodies and their associated neurite network over the following 24 h. This neuronal damage was quantitated with a colorimetric assay using the metabolic dye 3-(4,5-dimethylthiazol-2-yl)-...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(90)90101-9
更新日期:1990-01-01 00:00:00