Abstract:
:Infection with the protozoan parasite Toxoplasma gondii is transmitted to humans from infected animals by tissue cysts and oocysts excreted by cats. Immunization with inactivated parasites or recombinant proteins has at best shown partial protection. We constructed a plasmid expressing the SAG1 surface antigen of T. gondii, p1tPASAG1, and showed that animals immunized with the plasmid produce anti-SAG1 antibodies which recognize the native SAG1. Mice immunized with p1tPASAG1 showed 80 to 100% protection against challenge with the non-cyst-producing, virulent RH isolate, compared to an 80% mortality in mice immunized with empty plasmid, which is the greatest efficacy of any vaccine against T. gondii produced so far. The SAG1 molecule was analyzed for potential cytotoxic T-lymphocyte (CTL) epitopes, and four peptides with the best fit were synthesized. The ability of the peptides to stimulate gamma interferon production by CD8(+) T cells from p1tPASAG1-immunized mice was tested in an ELISPOT assay, and one new CTL epitope was identified. Adoptive transfer of CD8(+) T cells from p1tPASAG1-immunized to naïve mice showed partial protection. In conclusion, DNA vaccination with p1tPASAG1 gave effective protection in mice against T. gondii infection and the protection could be adoptively transferred by purified CD8(+) T cells.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Nielsen HV,Lauemøller SL,Christiansen L,Buus S,Fomsgaard A,Petersen Ekeywords:
subject
Has Abstractpub_date
1999-12-01 00:00:00pages
6358-63issue
12eissn
0019-9567issn
1098-5522journal_volume
67pub_type
杂志文章abstract::Cell-mediated immunity is critical in host resistance against the pathogenic fungus Histoplasma capsulatum. To explore the role of L3T4+ T cells in protection of mice against H. capsulatum infection, we examined the effect of in vivo treatment with anti-L3T4 monoclonal antibody (MAb) GK1.5 on the course of murine diss...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.7.1685-1691.1988
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journal_title:Infection and immunity
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doi:10.1128/IAI.43.1.139-142.1984
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.7.2629-2639.1997
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.1.238-246.1976
更新日期:1976-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.4.1328-1335.1994
更新日期:1994-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.1092-1101.1983
更新日期:1983-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.5.1559-1564.1996
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.2.253-259.1979
更新日期:1979-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1547-1553.1998
更新日期:1998-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.891-898.1995
更新日期:1995-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.299-301.1984
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.10.6271-6275.2001
更新日期:2001-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.1.133-138.1983
更新日期:1983-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00386-05
更新日期:2006-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-09-18 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01218-07
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
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更新日期:2018-09-21 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.3.1131-1134.1997
更新日期:1997-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1997-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.28.1.254-257.1980
更新日期:1980-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.3.1107-1115.1999
更新日期:1999-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1113-1120.2002
更新日期:2002-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.2.531-535.1984
更新日期:1984-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.800-808.1982
更新日期:1982-03-01 00:00:00