Abstract：
OBJECTIVES:To demonstrate the effectiveness of ultrarapid cryopreservation in human embryos. DESIGN:Retrospective study. SETTING:An IVF unit of a medical center. PATIENT(S):All cases received ultrarapid freezing (URF) of embryos and frozen embryo transfer. INTERVENTION(S):Embryos were placed in phosphate-buffered saline (PB1) + 20% maternal serum (MS) for 5 minutes. Embryos were loaded to the straws containing PB1 + MS + 0.25 M sucrose (SUC) + 3.5 M dimethyl sulfoxide for 3 minutes, and then were stored in a liquid nitrogen tank. Embryos were thawed in a 37 degrees C water for 6 seconds and then cultured in PB1 + MS + SUC for 10 minutes. Embryos were transferred into PB1 + MS for 5 minutes and were transferred into patients. MAIN OUTCOME MEASURE(S):The embryo grades before and after URF, the survival and transferred embryo numbers, and the pregnancy and abortion rates were analyzed. RESULT(S):A total of 1,582 embryos were thawed, of which 1,273 (80.5%) embryos were transferred and 1,032 (65.2%) embryos survived with > or = 50% intact blastomere. The embryo numbers with grade I or II before and after freezing and thawing were 1,110 (70.2%) and 790 (50.0%). The mean embryo numbers per transfer was 5.0. Twenty-eight pregnancies (11.4% per transfer) were established, which included 5 miscarriages, 1 ectopic pregnancy, 4 preterm and 18 term deliveries. CONCLUSION(S):Ultrarapid freezing is worthy of reconsideration for embryo cryopreservation.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Hsieh YY,Tsai HD,Chang CC,Chang CC,Lo HY,Lai ACdoi
10.1016/s0015-0282(99)00257-5keywords:
subject
Has Abstractpub_date
1999-08-01 00:00:00pages
253-6issue
2eissn
0015-0282issn
1556-5653pii
S0015-0282(99)00257-5journal_volume
72pub_type
杂志文章abstract：:Neutrophil migration did not change during normal menstrual cycles but was significantly reduced during controlled ovarian hyperstimulation (COH). Correlations between neutrophil migration and serum hormone concentrations could not be established. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.05.091
更新日期：2004-12-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of curcumin on aflatoxin-induced biochemical changes in testis of mice. DESIGN:Controlled research laboratory study. SETTING:Aflatoxin was obtained by growing Aspergillus parasiticus in SMKY liquid medium. Pure curcumin (97% purity) was purchased from Hi-Media Laboratories Pvt. Ltd...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.11.053
更新日期：2009-02-01 00:00:00
abstract：:Nine anovulatory patients with hypothalamic-pituitary dysfunction were treated with d-Trp6-luteinizing hormone-releasing hormone, an analog with far greater gonadotropin-releasing activity than luteinizing hormone-releasing hormone. Four of eight patients, who were formerly unsuccessfully treated with clomiphene, huma...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：
更新日期：1978-04-01 00:00:00
abstract：OBJECTIVE:To develop a rapid, sensitive, and reproducible hypoosmotic swelling test kit for the assessment of plasma membrane integrity of human sperm in vitro. DESIGN:Prospective comparison of results with the World Health Organization (WHO) method, performed simultaneously. SETTING:Infertility center in a major cit...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.02.003
更新日期：2008-01-01 00:00:00
abstract：:An attempt was made to develop an interclass somatic cell nuclear transfer method as an alternative means of establishing chicken embryonic stem cells. Chicken-to-cattle interclass embryos that activated calcium ionophore, cycloheximide, and cytochalasin D were developed into blastocysts, and the developing interclass...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.06.019
更新日期：2004-10-01 00:00:00
abstract：OBJECTIVE:To analyze embryo morphology in relation to the corresponding chromosomal status, in order to evaluate the effects of aneuploidy over fragmentation, delayed cleavage, and arrested cleavage. DESIGN:Retrospective study. SETTING:Reproductive Medicine Unit, Società Italiana di Studi di Medicina della Riproduzio...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.07.1512
更新日期：2007-03-01 00:00:00
abstract：:A double-blind randomized study was performed in two groups of eight normally cycling patients: group I received 10 mg/d of RU486 for 4 days from the date of ovulation and group II received a placebo. On day +5, cytosol and endometrial estrogen receptors (ERs), and progesterone receptors (PRs) were analyzed by radioli...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(16)54183-1
更新日期：1991-03-01 00:00:00
abstract：OBJECTIVE:To examine the expression of the heat shock protein hsp70-2, and the possible relationship with the pathogenesis of male infertility. DESIGN:Prospective study. SETTING:Reproductive testing laboratory in a university hospital. PATIENT(S):Men undergoing testicular biopsy during an investigation of subfertili...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(01)02892-8
更新日期：2001-12-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect of metformin in polycystic ovary syndrome (PCOS). As follow-up on a previous paper describing hormonal and metabolic factors, this paper focuses on correlations between adiponectin and anthropometric, hormonal, and metabolic factors in PCOS and the effect of metformin. DESIGN:Randomize...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2010.01.057
更新日期：2010-11-01 00:00:00
abstract：OBJECTIVE:To determine whether absence of fertilization in IVF associated with an acrosomal enzyme defect (hyaluronidase deficiency) results from a simple mechanical block to sperm penetration or from a more serious sperm abnormality. DESIGN:Nonrandomized, prospective study. SETTING:Toronto Center for Advanced Reprod...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
abstract：OBJECTIVE:To compare the effects of serum with those of Albuminar-5 (Armour Pharmaceutical Co., Eastbourne, Sussex, United Kingdom) as medium supplements to Earl's balanced salt solution (EBSS) for IVF and subsequent embryo development. DESIGN:A retrospective study. Gametes and embryos from 318 patients were cultured ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)57978-3
更新日期：1995-12-01 00:00:00
abstract：OBJECTIVE:To evaluate the impact of dynamic in vitro culture on initiation of early follicular growth in prepubertal mouse ovaries. DESIGN:Ovaries from 8-day-old BALB/c mice were cultured either in a dynamic system (n=28) or in a static system (n=20) for 4 days. Uncultured 8-day-old (n=9) or 12-day-old (n=17) ovaries ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.05.038
更新日期：2014-09-01 00:00:00
abstract：OBJECTIVE:To examine the impact of a recent surgery on development of endometriosis-related adhesions in a chimeric model and to determine the therapeutic efficacy of pioglitazone (PIO). DESIGN:Human endometrial biopsies were maintained in E(2) with or without PIO for 24 h before intraperitoneal injection into immunoc...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.09.017
更新日期：2011-03-15 00:00:00
abstract：OBJECTIVE:To assess the variability of antimüllerian hormone (AMH) concentrations in women with "adequate ovarian reserve" during unstimulated menstrual cycles and to determine the impact on clinical classifications. DESIGN:Pilot cohort study. SETTING:Private fertility clinic. PATIENT(S):Twelve consecutive women (ag...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.01.132
更新日期：2013-05-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect of melatonin both on the ovaries of pinealectomized female rats through histomorphometric analysis and on steroid receptors, proliferating cell nuclear antigen (PCNA), and vascular endothelial growth factor (VEGF) expression. DESIGN:Experimental study. SETTING:Federal University of Sã...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.04.042
更新日期：2011-03-15 00:00:00
abstract：:The effect on sperm motility of nonoxynol-9 chlorhexidine diacetate was compared in semen and cervical mucus. Both compounds had similar spermicidal potency in semen, abolishing sperm motility within 3 minutes at 0.5 mg/ml. When these compounds were allowed to diffuse into mucus, the subsequent survival of sperm in th...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)49165-x
更新日期：1986-02-01 00:00:00
abstract：OBJECTIVE:Hepatic lipase activity is involved in the removal of cholesterol and phospholipid from plasma high-density lipoprotein (HDL) to the liver. Changes in hepatic lipase are responsible for some lipoprotein modifications observed during estrogen administration (i.e., increased HDL levels). The aim of this study w...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54361-1
更新日期：1991-06-01 00:00:00
abstract：OBJECTIVE:To determine if the solubilized human zona pellucida (ZP)-induced acrosome reaction is mediated by protein kinases. DESIGN:Capacitated spermatozoa were incubated with inhibitors of cyclic adenosine 3':5'-monophosphate (cAMP)-dependent kinase (KT5720), Ca(2+)- and phospholipid-dependent kinase (Calphostin C),...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：OBJECTIVE:To determine whether endometrial hCG infusion at the time of human blastocyst transfer impacts implantation rates. DESIGN:Randomized double-blinded placebo-controlled trial. SETTING:Academic. PATIENT(S):Infertile couples with the female partner less than 43 years old (n = 300) undergoing fresh or frozen ET...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2014.08.006
更新日期：2014-12-01 00:00:00
abstract：:The serial measurement of serum hCG concentrations is an important clinical tool used to differentiate normal from abnormal pregnancies. At hCG values below which ultrasonography can be diagnostic, the comparison of a patient's hCG dynamics to those of established expected curves of rise or fall can aid the clinician ...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2012.09.014
更新日期：2012-11-01 00:00:00
abstract：OBJECTIVE:To determine whether differences existed in mood and coping styles among fertile men, oligoasthenospermic men, or euspermic men whose wives were undergoing ovulation stimulation with clomiphene and IUI. DESIGN:A cross-sectional research design. SETTING:Hospital-based academic fertility clinic. PATIENT(S):3...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(00)01511-9
更新日期：2000-10-01 00:00:00
abstract：OBJECTIVE:To characterize sperm parameters from thawed semen samples of men with different cancers who cryopreserved semen before oncologic therapy. DESIGN:Retrospective cohort study. SETTING:Tertiary academic medical center. PATIENT(S):1,010 semen samples collected between 1994 to 2010. INTERVENTION(S):None. MAIN...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.09.031
更新日期：2013-02-01 00:00:00
abstract：OBJECTIVE:To evaluate the efficacy of a dry versus humidified incubator on human embryo development ex vivo. DESIGN:Prospective, double-blind, randomized, controlled trial. SETTING:Private fertility centers. PATIENT(S):A total of 297 women undergoing in vitro fertilization randomized into two groups. INTERVENTION(S...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.fertnstert.2017.05.036
更新日期：2017-08-01 00:00:00
abstract：:A prospective longitudinal and standardized study is presented, dealing with ultrasonographic and hormonal characteristics of the luteinized unruptured follicle (LUF) syndrome. Among 600 cycles monitored in 270 infertility patients, 40 cycles in 27 patients showed no evidence of follicle rupture, in spite of signs of ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)48494-3
更新日期：1985-04-01 00:00:00
abstract：:Hysteroscopic visualization of the endometrium after 6 months of antitubercular therapy showed an improvement in the mucosal morphology. A closer visualization at increased magnification was helpful in demonstrating the remnants of a healing tubercular pathology after antitubercular therapy. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.07.1346
更新日期：2008-03-01 00:00:00
abstract：:Blood was collected from 20 patients with varicocele (15 in the infertile group and 5 in the incidental varicocele group) at the time of high ligation by insertion of a catheter into the internal spermatic vein toward the renal side. Simultaneously, blood was obtained from the antecubital vein. The levels of cortisol,...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)46043-7
更新日期：1982-02-01 00:00:00
abstract：:In the present study, we investigated the impact of metformin therapy on in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) outcomes in patients with polycystic ovary syndrome (PCOS). Metformin does not lead to any improvement in IVF/ICSI outcomes among patients with PCOS. ...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2005.03.043
更新日期：2005-09-01 00:00:00
abstract：OBJECTIVE:To quantify mitochondrial DNA using real-time PCR in women with premature ovarian failure (POF) and a control group. DESIGN:Prospective study. SETTING:Genome Research Center for Reproductive Medicine and Infertility, Korea Ministry of Health & Welfare. PATIENT(S):Thrity patients with POF and 30 control ind...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2005.06.036
更新日期：2005-12-01 00:00:00
abstract：OBJECTIVE:To assess cycle outcomes when antimüllerian hormone (AMH) is ultralow (≤0.16 ng/mL) and to determine which parameters contribute to the probability of cycle cancellation and/or outcome. DESIGN:Retrospective analysis. SETTING:Not applicable. PATIENT(S):5,087 (7.3%) fresh and 243 (1.5%) thawed cycles with ul...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.10.004
更新日期：2016-02-01 00:00:00
abstract：OBJECTIVE:To determine if the addition of fibrin sealant to incision closure in a rabbit uterine horn myomectomy model affects adhesion formation or strength of incision closure. DESIGN:Prospective randomized controlled trial. SETTING:Academic research center. ANIMAL(S):New Zealand white female rabbits. INTERVENTIO...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.07.1337
更新日期：2006-04-01 00:00:00
