Abstract：
OBJECTIVE:To determine whether absence of fertilization in IVF associated with an acrosomal enzyme defect (hyaluronidase deficiency) results from a simple mechanical block to sperm penetration or from a more serious sperm abnormality. DESIGN:Nonrandomized, prospective study. SETTING:Toronto Center for Advanced Reproductive Technology, a tertiary referral center for infertility associated with The University of Toronto. PATIENTS:One hundred twenty-two couples about to undergo intracytoplasmic sperm injection (ICSI) were selected. Thirty-six of the studied couples had failed to fertilize in prior IVF cycles. INTERVENTIONS:Hyaluronidase activity was measured in the semen samples provided for ICSI using a zymogenic assay. Intracytoplasmic sperm injection was performed in all couples using standard techniques. RESULTS:Forty-eight of 122 semen samples had poor of absent semen hyaluronidase activity. All 48 samples resulted in successful fertilization with ICSI in the present study. The average fertilization rate per oocyte was 59.43% in couples in whom the partner had low semen hyaluronidase activity and 55.85% in whom the male had normal hyaluronidase activity. The ET rate per cycle was 100% and 95% and pregnancy rates per cycles were 26% and 25% in cycles with poor and normal semen hyaluronidase activity, respectively. Unlike routine IVF, no statistical correlation was found between semen hyaluronidase activity and the fertilization rate in ICSI. CONCLUSION:Our results indicates that semen hyaluronidase deficiency is associated with a simple mechanical block to fertilization. In addition, the measurement of semen hyaluronidase activity can provide a reliable means for selecting couples who would benefit from ICSI.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Abdul-Aziz M,Meriano J,Casper RFsubject
Has Abstractpub_date
1996-05-01 00:00:00pages
977-80issue
5eissn
0015-0282issn
1556-5653pii
S0015-0282(16)58272-7journal_volume
65pub_type
杂志文章abstract：OBJECTIVE:To study the apparent association between interstitial fallopian tube polyps and isthmic tubal dilatation. DESIGN:Retrospective clinical study. SETTING:Tertiary academic medical center. PATIENT(S):Sixty-five patients with normal hysterosalpingograms and 40 patients with interstitial tubal polyps (25 unilat...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.10.049
更新日期：2005-05-01 00:00:00
abstract：:Specialist training in reproductive medicine within Europe continues to evolve. Recent revisions, updates, and initiatives have helped to refine the core educational needs for the specialist trainee. ...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2015.05.025
更新日期：2015-07-01 00:00:00
abstract：OBJECTIVE:To determine the optimal GnRH agonist dose for triggering of oocyte maturation in oocyte donors. DESIGN:Single-center, randomized, parallel, investigator-blinded trial. SETTING:IVFMD, My Duc Hospital, Ho Chi Minh City, Vietnam. PATIENT(S):One hundred sixty-five oocyte donors (aged 18-35 years, body mass in...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2015.10.014
更新日期：2016-02-01 00:00:00
abstract：OBJECTIVE:To present the set of reasons for and against fertility treatment for a very young patient. DESIGN:An expert opinion based on clinical experience. SETTING:An academic-affiliated fertility clinic situated in East Jerusalem. PATIENT(S):A 16-year-old married teenager with 2 years' duration of infertility due ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.02.172
更新日期：2008-11-01 00:00:00
abstract：OBJECTIVE:To investigate the role(s) of hyaluronan synthase 2 (HAS2) and hyaluronan in disease progression of endometriosis and epidermal growth factor (EGF)-induced motility changes of endometriotic cells. DESIGN:A case-control experimental study and in vitro primary cell culture study. SETTING:University hospital-a...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.05.005
更新日期：2020-10-01 00:00:00
abstract：OBJECTIVE:To investigate whether sphingosine-1-phosphate (S1P), an apoptosis-inhibitor, would be able to protect ovarian follicles from chemotherapy-induced cell death in vivo. DESIGN:Animal study. SETTING:Academic medical center. ANIMAL(S):Twenty female mice. INTERVENTION(S):Twenty mice were randomly assigned into...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.06.020
更新日期：2007-01-01 00:00:00
abstract：:Embryo transfer is facilitated with ultrasound guidance. The 16 ultrasound-guided transfers were rated easier, and there was less catheter distortion, in comparison with 12 transfers guided by "clinical feel." The benefits we ascribe to ultrasound guidance are as follows: (1) transfers can be done with the patient sup...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)48317-2
更新日期：1985-01-01 00:00:00
abstract：:A study was undertaken comparing the outcomes of 30 women with infertility due to untreated severe (grade IV) pelvic endometriosis with a comparable series of 28 women whose infertility was caused solely by irreversible tubal disease. There were no significant differences in either the follicular phase or luteal phase...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60078-x
更新日期：1988-08-01 00:00:00
abstract：:Two male cousins with partial 21-hydroxylase deficiency presented with bilateral testicular masses and infertility. In both cases, the testicular masses, consisting of adrenocorticotropic hormone-dependent pluripotential cells, were thought to play a major etiologic role in infertility. The administration of very smal...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)47485-6
更新日期：1983-12-01 00:00:00
abstract：OBJECTIVE:To describe a woman with Kallmann's syndrome who was treated successfully with highly purified FSH to achieve ovulation induction and pregnancy. DESIGN:Case report. SETTING:University hospital. PATIENT(S):A 32-year-old woman with Kallmann's syndrome who had been treated with oral contraceptives to prime se...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(99)00535-x
更新日期：2000-02-01 00:00:00
abstract：:Nonaromatizable androgens, administered in high doses to an adult patient with partial androgen insensitivity, failed to result in a change in phallic size despite a clear decline in SHBG and gonadotropin levels. These findings raise the question of differential tissue sensitivity to androgens. Because ancillary labor...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)56114-7
更新日期：1993-08-01 00:00:00
abstract：OBJECTIVE:To standardize and validate an assay for reactive oxygen species (ROS) in human semen. DESIGN:ROS levels assayed in blanks, negative and positive control samples (30% H2O2), and human semen, with the use of a luminol-based chemiluminescence assay measured in a single tube luminometer. SETTING:Andrology labo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.09.009
更新日期：2014-12-01 00:00:00
abstract：OBJECTIVES:To test the hypothesis that fertility is reduced in baboons with endometriosis. DESIGN:Prospective controlled follow-up study. SETTING:Institute of Primate Research, Nairobi Kenya. SUBJECTS:Seventy-one baboons, including 34 controls (normal pelvis) and 37 animals with histologically proven endometriosis (...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：OBJECTIVE:To describe a live birth obtained in Italy after autologous orthotopic transplantation of cryopreserved ovarian cortical tissue. DESIGN:Case report. SETTING:University department of gynecology and obstetrics, reproductive medicine and IVF unit. PATIENT(S):A 29-year-old patient affected by β-thalassemia (in...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.09.029
更新日期：2013-01-01 00:00:00
abstract：:The basal body temperature (BBT) charts in pregnancy cycles of 110 consecutive women who became pregnant by a single heterologous insemination were studied. The occurrence of ovulation was proved by the pregnancies in each case. All of these pregnancy charts showed a biphasic pattern, and most followed one of three st...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)46577-5
更新日期：1982-10-01 00:00:00
abstract：OBJECTIVE:To describe ovulation induction and pregnancy outcome in a unique model of genetically determined combined pituitary hormone deficiency (CPHD), with respect to the necessity for GH substitution therapy. DESIGN:Case report. SETTING:Academic units. PATIENT(S):Two patients with childhood onset of CPHD (GH, PR...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.03.026
更新日期：2004-08-01 00:00:00
abstract：OBJECTIVE:To investigate the mutation profiles and clinical characteristics of Chinese males with isolated hypogonadotropic hypogonadism (IHH) and discover new pathogenic genes that cause IHH. DESIGN:A gene panel, including 31 known IHH genes and 52 candidate genes, was used to perform semiconductor next-generation se...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2018.04.010
更新日期：2018-08-01 00:00:00
abstract：OBJECTIVE:To study the ovarian reserve, ovarian response to gonadotropins, and oocyte maturity in women with cancer undergoing in vitro fertilization (IVF) before chemotherapy or radiotherapy. DESIGN:Case-control study. SETTING:University teaching hospital. PATIENT(S):We evaluated all women with malignancy who under...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2011.04.070
更新日期：2011-07-01 00:00:00
abstract：:In this prospective case-control study, we determined the role of surface-enhanced laser desorption and ionization time-of-flight mass spectrometry in the detection of histologically proven endometriosis. In the plasma of the group with endometriosis, there were 20 different protein peaks, and the classifier showed a ...
journal_title：Fertility and sterility
pub_type： 信件
doi：10.1016/j.fertnstert.2006.08.095
更新日期：2007-04-01 00:00:00
abstract：OBJECTIVE:To assess the expression and cellular distribution of pregnancy-associated plasma protein-A (PAPP-A) and major basic protein (MBP) in human ovarian tissue during the menstrual cycle. DESIGN:Ovarian tissues (n = 50) and corpora lutea (n = 18) were obtained from patients undergoing hysterectomy/oophorectomy fo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(03)00077-3
更新日期：2003-05-01 00:00:00
abstract：:The effect of administration of a superactive and long-acting analog of luteinizing hormone-releasing hormone (LH-RH), D-Trp6-LH-RH, in doses of 0.05 or 1 microgram/day for 10 days on the hypothalamic-pituitary-gonadal axis was studied in immature female rats. Treatment with a 0.05-microgram dose of analog produced fe...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)44061-6
更新日期：1979-06-01 00:00:00
abstract：OBJECTIVE:To determine whether fallopian sperm perfusion significantly improves pregnancy rates over intrauterine insemination (IUI) in infertile patients. DESIGN:Prospective, randomized, controlled trial and metaanalysis of the literature. SETTING:University-based infertility practice. PATIENT(S):Patients undergoin...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,meta分析,随机对照试验
doi：10.1016/s0015-0282(99)00068-0
更新日期：1999-05-01 00:00:00
abstract：:The penetration of a gonadotropin-releasing hormone agonist (GnRH-a), Buserelin (Hoechst AG, Frankfurt, West Germany), into human follicular fluids (FF) was studied by means of a radioimmunoassay (RIA) and a bioassay. Acute nasal administration of a therapeutic dose of Buserelin (300 and 600 micrograms) before the ovu...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60852-x
更新日期：1989-08-01 00:00:00
abstract：OBJECTIVE:To investigate whether a history of previous ectopic pregnancy is an independent risk factor for failure of methotrexate therapy in subsequent ectopic pregnancies and whether the previous treatment method influenced failure. SETTING:Inner-city teaching hospital. DESIGN:Retrospective case review. PATIENT(S)...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2003.09.070
更新日期：2004-05-01 00:00:00
abstract：:Pelvic adhesion formation represents a major problem following fallopian tube surgery for infertility. Intraperitoneal dextran may prevent pelvic adhesions. Extensive personal clinical experience (W. H. U.) with intraperitoneal dextran organ-flotation on completion of tubal and ovarian surgery has appeared to limit ad...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)43765-9
更新日期：1979-01-01 00:00:00
abstract：OBJECTIVE:To review the mechanisms of T replacement therapy's inhibition of spermatogenesis and current therapeutic approaches in reproductive aged men. DESIGN:Review of published literature. SETTING:PubMed search from 1990-2012. PATIENT(S):PubMed search from 1990-2012. INTERVENTION(S):A literature review was perfo...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2012.10.052
更新日期：2013-03-01 00:00:00
abstract：OBJECTIVE:To evaluate if the presence of endometriomas impacts on the ovarian reserve as evaluated with antimüllerian hormone (AMH). DESIGN:Systematic review and meta-analysis. SETTING:Not applicable. PATIENT(S):Patients with unoperated endometriomas versus controls without endometriomas. INTERVENTION(S):Electronic...
journal_title：Fertility and sterility
pub_type： 杂志文章,meta分析
doi：10.1016/j.fertnstert.2018.06.025
更新日期：2018-10-01 00:00:00
abstract：:Midluteal phase corpora lutea (CL) obtained from women induced with 50 mg (n = 5), 100 mg (n = 5), and 150 mg (n = 5) of clomiphene citrate (CC) were measured for luteinizing hormone/human chorionic gonadotropin (LH/hCG) concentrations and cytosol progesterone (P) and 17 alpha-hydroxyprogesterone (17-OHP) and compared...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：OBJECTIVE:To evaluate the benefit of Y microdeletion testing. DESIGN:Retrospective analysis. SETTING:University-based male fertility clinic and genetics laboratory. PATIENT(S):A total of 1,591 men with sperm concentrations less than 5 million sperm/mL. INTERVENTION(S):Semen analysis, Y microdeletion testing, microd...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.09.006
更新日期：2010-10-01 00:00:00
abstract：OBJECTIVE:To determine if increases in core body temperature preceding most hot flashes are caused by increased metabolic rate, peripheral vasoconstriction, or central noradrenergic activation. DESIGN:Laboratory physiological study. SETTING:University medical center. PATIENT(S):Fourteen healthy, postmenopausal women...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(98)00137-x
更新日期：1998-08-01 00:00:00
