Intracytoplasmic sperm injection for treatment of infertility due to acrosomal enzyme deficiency.


OBJECTIVE:To determine whether absence of fertilization in IVF associated with an acrosomal enzyme defect (hyaluronidase deficiency) results from a simple mechanical block to sperm penetration or from a more serious sperm abnormality. DESIGN:Nonrandomized, prospective study. SETTING:Toronto Center for Advanced Reproductive Technology, a tertiary referral center for infertility associated with The University of Toronto. PATIENTS:One hundred twenty-two couples about to undergo intracytoplasmic sperm injection (ICSI) were selected. Thirty-six of the studied couples had failed to fertilize in prior IVF cycles. INTERVENTIONS:Hyaluronidase activity was measured in the semen samples provided for ICSI using a zymogenic assay. Intracytoplasmic sperm injection was performed in all couples using standard techniques. RESULTS:Forty-eight of 122 semen samples had poor of absent semen hyaluronidase activity. All 48 samples resulted in successful fertilization with ICSI in the present study. The average fertilization rate per oocyte was 59.43% in couples in whom the partner had low semen hyaluronidase activity and 55.85% in whom the male had normal hyaluronidase activity. The ET rate per cycle was 100% and 95% and pregnancy rates per cycles were 26% and 25% in cycles with poor and normal semen hyaluronidase activity, respectively. Unlike routine IVF, no statistical correlation was found between semen hyaluronidase activity and the fertilization rate in ICSI. CONCLUSION:Our results indicates that semen hyaluronidase deficiency is associated with a simple mechanical block to fertilization. In addition, the measurement of semen hyaluronidase activity can provide a reliable means for selecting couples who would benefit from ICSI.


Fertil Steril


Fertility and sterility


Abdul-Aziz M,Meriano J,Casper RF


Has Abstract


1996-05-01 00:00:00














  • Intestinal endometriosis complicated by ileal perforation after initiation of gonadotropin-releasing hormone agonist therapy.

    abstract:OBJECTIVE:To report a rare complication of GnRH agonist therapy for intestinal endometriosis. DESIGN:Case report. SETTING:University hospital. PATIENT(S):A 45-year-old nulliparous Japanese woman with catamenial digestive symptoms. INTERVENTION(S):GnRH agonist therapy. MAIN OUTCOME MEASURE(S):Acute abdomenal crisis...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Saito S,Murakami T,Suzuki K,Terada Y,Fukushima K,Moriya T

    更新日期:2007-10-01 00:00:00

  • Polyovular follicles associated with human in vitro fertilization.

    abstract::Polyovular follicles were found in 61 (24%) of 251 laparoscopies performed for in vitro fertilization. Of 898 follicles that contained at least one oocyte, 76 (8%) were polyovular. When the oocytes from polyovular follicles were assessed by morphologic criteria, it was found that 46 of the follicles contained oocytes ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Dandekar PV,Martin MC,Glass RH

    更新日期:1988-03-01 00:00:00

  • Maturation and apoptosis of human oocytes in vitro are age-related.

    abstract:OBJECTIVE:To study the morphological changes of apoptotic oocytes, and rates of in vitro maturation and apoptosis of human oocytes in relation to age. DESIGN:Prospective comparative study. SETTING:Reproductive medicine center. PATIENT(S):Women undergoing surgery for ovarian cysts. INTERVENTION(S):Oocytes were incub...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Wu J,Zhang L,Wang X

    更新日期:2000-12-01 00:00:00

  • Induction of endometrial cycles and ovulation in a woman with combined 17alpha-hydroxylase/17,20-lyase deficiency due to compound heterozygous mutations on the p45017alpha gene.

    abstract:OBJECTIVE:To describe the case of a Japanese woman with combined 17alpha-hydroxylase/17,20-lyase deficiency (congenital adrenal hyperplasia type V) and to discuss possible therapeutic procedures in such patients. DESIGN:Case report. SETTING:University hospital. PATIENT(S):A 26-year-old woman with secondary amenorrhe...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Matsuzaki S,Yanase T,Murakami T,Uehara S,Nawata H,Yajima A

    更新日期:2000-06-01 00:00:00

  • Placental expression profiling in preeclampsia: local overproduction of hemoglobin may drive pathological changes.

    abstract:OBJECTIVE:To create a library enriched in cDNAs from preeclamptic placentas to print onto microarrays for placental profiling of preeclampsia (PE) and high risk pregnancies. DESIGN:Prospective study. SETTING:University women's clinic and academic research laboratory. PATIENT(S):Ten patients with PE, 5 with PE and bi...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Centlow M,Carninci P,Nemeth K,Mezey E,Brownstein M,Hansson SR

    更新日期:2008-11-01 00:00:00

  • Secondary hypogonadism in hemochromatosis.

    abstract::Hemochromatosis is a rare disorder of iron storage. This report illustrates a case of hypogonadotropic-hypogonadism in a female with biopsy-proven hemochromatosis. Dynamic pituitary and gonadal testing revealed subnormal gonadotropin responses to gonadotropin-releasing hormone (GnRH) but normal ovarian reserve, as sho...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Meyer WR,Hutchinson-Williams KA,Jones EE,DeCherney AH

    更新日期:1990-10-01 00:00:00

  • Transvaginal intratubal methotrexate treatment of ectopic pregnancy.

    abstract:OBJECTIVE:To evaluate the efficacy of transvaginal intratubal methotrexate (MTX) treatment of tubal ectopic pregnancy (EP). SETTING:Outpatient setting in University Hospital. PATIENTS:Forty women with early EP and rising serum beta-human chorionic gonadotropin (beta-hCG) levels. INTERVENTION:Transvaginal intratubal ...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章


    authors: Tulandi T,Atri M,Bret P,Falcone T,Khalife S

    更新日期:1992-07-01 00:00:00

  • Effects of aromatase inhibitors on proliferation and apoptosis in eutopic endometrial cell cultures from patients with endometriosis.

    abstract:OBJECTIVE:To study the effect of letrozole (Let) and anastrozole (Anas) on apoptosis and cell proliferation in epithelial endometrial cells (EEC) from patients with endometriosis (EDT). DESIGN:Prospective study. SETTING:Research institute and clinical fertility center. PATIENT(S):Eighteen women with untreated EDT. ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Meresman GF,Bilotas M,Abello V,Buquet R,Tesone M,Sueldo C

    更新日期:2005-08-01 00:00:00

  • Tumor necrosis factor-alpha regulates vascular endothelial growth factor secretion by human oviductal epithelial cells and stromal fibroblasts.

    abstract::Tumor necrosis factor-alpha stimulates the secretion of vascular endothelial growth factor by cultured human oviductal epithelial cells and stromal fibroblasts. Tumor necrosis factor-alpha-stimulated vascular endothelial growth factor secretion by these cells may control oviductal fluid secretion by regulating vascula...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Nasu K,Itoh H,Yuge A,Nishida M,Kawano Y,Narahara H

    更新日期:2007-01-01 00:00:00

  • Embryo morphology and development are dependent on the chromosomal complement.

    abstract:OBJECTIVE:To analyze embryo morphology in relation to the corresponding chromosomal status, in order to evaluate the effects of aneuploidy over fragmentation, delayed cleavage, and arrested cleavage. DESIGN:Retrospective study. SETTING:Reproductive Medicine Unit, Società Italiana di Studi di Medicina della Riproduzio...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Magli MC,Gianaroli L,Ferraretti AP,Lappi M,Ruberti A,Farfalli V

    更新日期:2007-03-01 00:00:00

  • Blood carbon dioxide tension changes during hysteroscopy.

    abstract::During hysteroscopy the uterus may be distended with carbon dioxide (CO2), nitrous oxide (N2O), or Hyskon (a high molecular weight dextran). An initial study in 27 patients (group 1) using arterialized venous blood samples demonstrated rises in carbon dioxide tension (PCO2) when N2O was insufflated by using a laparosc...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Hulf JA,Corall IM,Knights KM,Strunin L,Newton JR

    更新日期:1979-08-01 00:00:00

  • Public perspectives on placing age limits on men and women seeking fertility treatment.

    abstract:OBJECTIVE:To assess public attitudes toward placing age limits on men and women seeking fertility treatment. DESIGN:Cross-sectional web-based survey. SETTING:Not applicable. PATIENT(S):A nationally representative sample of 1,427 adults in the United States. INTERVENTION(S):Not applicable. MAIN OUTCOME MEASURE(S):S...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Lee MS,Farland LV,Thomas AM,Ginsburg ES

    更新日期:2019-03-01 00:00:00

  • Next-generation sequencing reveals differentially expressed small noncoding RNAs in uterine leiomyoma.

    abstract:OBJECTIVE:To determine the expression profile of small noncoding RNAs (sncRNAs) in leiomyoma, which has not been investigated to date. DESIGN:Laboratory-based investigation. SETTING:Academic center. PATIENT(S):Women undergoing hysterectomy for benign indications. INTERVENTION(S):Next-generation sequencing and scree...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Chuang TD,Xie Y,Yan W,Khorram O

    更新日期:2018-05-01 00:00:00

  • Relationship and parenthood in IVF couples with twin and singleton pregnancies compared with spontaneous singleton primiparous couples--a prospective 5-year follow-up study.

    abstract:OBJECTIVE:To analyze relationship and parenthood in IVF twin couples, IVF singleton couples, and spontaneously pregnant primiparous couples. SETTING:Southeast region of Sweden. DESIGN:Prospective case-control study. PATIENT(S):In total, 24 women and men who became pregnant with twins, consecutively, and expected the...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Sydsjö G,Wadsby M,Sydsjö A,Selling KE

    更新日期:2008-03-01 00:00:00

  • Expression and activation of the membrane-cytoskeleton protein ezrin during the normal endometrial cycle.

    abstract:OBJECTIVE:To examine total ezrin expression (ezrin and phospho-ezrin) through the normal endometrial cycle and to correlate ezrin activation and localization with cytologic changes. DESIGN:Experimental laboratory study. SETTING:University medical centers. PATIENT(S):Reproductive-age women. INTERVENTION(S):A total o...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Tan O,Ornek T,Fadiel A,Carrick KS,Arici A,Doody K,Carr BR,Naftolin F

    更新日期:2012-01-01 00:00:00

  • Embryonic adhesion is not affected by endometrial leptin receptor gene silencing.

    abstract:OBJECTIVE:In rodents, evidence suggests that the leptin system is mandatory for embryonic implantation. We aimed to investigate the functional relevance of the endometrial leptin receptor (OB-R) in the adhesion phase of human implantation. DESIGN:We used an in vitro model for embryonic adhesion, composed of a human en...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Cervero A,Domínguez F,Horcajadas JA,Quiñonero A,Pellicer A,Simón C

    更新日期:2007-10-01 00:00:00

  • Spontaneous apoptosis of endometrial tissue is impaired in women with endometriosis.

    abstract:OBJECTIVE:To evaluate spontaneous apoptosis in single-cell suspensions of eutopic and ectopic endometrium from women with endometriosis and in eutopic endometrium from fertile controls without endometriosis. DESIGN:Paired specimens of eutopic and ectopic endometrial tissue from patients with endometriosis and eutopic ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Gebel HM,Braun DP,Tambur A,Frame D,Rana N,Dmowski WP

    更新日期:1998-06-01 00:00:00

  • Identification of low molecular weight immunosuppressor molecules in human in vitro fertilization supernatants predictive of implantation as a polyamine--possibly spermine.

    abstract::Suppressor activity in in vitro fertilization (IVF) culture medium correlates with successful implantation. High performance liquid chromatography fractionation revealed peak(s) of inhibitory activity in the 1,000- to 5,000-Da molecular weight range. Inhibitory activity was dependent on the presence of fetal bovine se...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Lea RG,Daya S,Clark DA

    更新日期:1990-05-01 00:00:00

  • Impact of obesity on women's health.

    abstract:OBJECTIVE:To review the impacts of obesity on women's reproductive health and fertility. DESIGN:Literature review. SETTING:Academic medical center. PATIENT(S):Forty articles are referenced. The number of patients evaluated in each of the studies varies from 33 to 213,208. INTERVENTION(S):Articles were identified fr...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Lash MM,Armstrong A

    更新日期:2009-05-01 00:00:00

  • Combined use of progesterone and human chorionic gonadotropin determinations for differential diagnosis of very early pregnancy.

    abstract::Progesterone (P) level and daily change in human chorionic gonadotropin (hCG) were determined in the serum of 307 patients with suspected ectopic pregnancy (EP). Of the viable intrauterine pregnancies (IUP), 99% had P values above 30 nmol/L, whereas 75% of the EP and 81% of the spontaneous abortions had P values less ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Hahlin M,Sjöblom P,Lindblom B

    更新日期:1991-03-01 00:00:00

  • A new concept for anastomosis of the fallopian tube: tissue reconstruction with nonpenetrating, arcuate, legged clips in the rat model.

    abstract:OBJECTIVE:To evaluate the titanium Kirsch-Zhu microclip microsurgical reanastomoses of the fallopian tubes. DESIGN:Compare the reanastomoses of the rat uterine horn between Kirsch-Zhu clips (Cushman Engineering, Albuquerque, NM) (group A) and conventional suture microsurgical techniques (group B). SETTING:Microsurger...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Tredway DR,Kirsch WM,Zhu YH,Weber K,Norburg M,Saukel GW,Seraj I

    更新日期:1994-09-01 00:00:00

  • Peroxisome proliferator-activated receptor gamma and early folliculogenesis during an acute hyperandrogenism condition.

    abstract::Acute hyperandrogenism decreases serum P levels and induces early apoptosis of antral follicles by a mechanism mediated by the peroxisome proliferator-activated receptor gamma system and independent of the steroidogenic acute regulator protein. ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Faut M,Elia EM,Parborell F,Cugnata NM,Tesone M,Motta AB

    更新日期:2011-01-01 00:00:00

  • The biologic significance of white blood cells in semen.

    abstract:OBJECTIVE:To analyze the data available on the biologic significance of white blood cells (WBC) in semen of infertility patients. DATA RESOURCES:The relevant literature was reviewed. RESULTS:It is not possible to identify reliably WBC by conventional sperm staining techniques. The peroxidase method is sufficient for ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审


    authors: Wolff H

    更新日期:1995-06-01 00:00:00

  • Treatment of cervical mucus with lectins: effect on sperm migration.

    abstract::Treatment of midcycle human cervical mucus with the lectins Ulex europaeus agglutinin I (UE), wheat germ agglutinin (WGA), and peanut agglutinin (PA) did not alter the ability of spermatozoa to enter and migrate through the mucus. These lectins form glycoconjugates by binding to L-fucose (UE), N-acetylglucosamine and ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Snyder MG,Zaneveld LJ

    更新日期:1985-11-01 00:00:00

  • Clinical management of mosaic results from preimplantation genetic testing for aneuploidy (PGT-A) of blastocysts: a committee opinion.

    abstract::Since the advent of preimplantation genetic testing for aneuploidy (PGT-A) in the 1990s, substantial changes in test methodology and technology now allow the detection and reporting of intermediate chromosome copy number (commonly referred to as mosaicism) for aneuploidy in a trophectoderm biopsy sample. Clinicians ar...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Practice Committee and Genetic Counseling Professional Group (GCPG) of the American Society for Reproductive Medicine. Electronic address:

    更新日期:2020-08-01 00:00:00

  • Prevalence, consequence, and significance of reverse cleavage by human embryos viewed with the use of the Embryoscope time-lapse video system.

    abstract:OBJECTIVE:To investigate the prevalence and potential causes of reverse cleavage (RC) by human early-cleavage embryos and its associations with embryonic development and implantation after transfer. DESIGN:Clinical retrospective cohort study. SETTING:Private fertility treatment center. PATIENT(S):A total of 126 cons...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Liu Y,Chapple V,Roberts P,Matson P

    更新日期:2014-11-01 00:00:00

  • The ReSTAGE Collaboration: defining optimal bleeding criteria for onset of early menopausal transition.

    abstract:OBJECTIVE:Criteria for staging the menopausal transition are not established. This article evaluates five bleeding criteria for defining early transition and provides empirically based guidance regarding optimal criteria. DESIGN/SETTING:Prospective menstrual calendar data from four population-based cohorts: TREMIN, Me...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,多中心研究


    authors: Harlow SD,Mitchell ES,Crawford S,Nan B,Little R,Taffe J,ReSTAGE Collaboration.

    更新日期:2008-01-01 00:00:00

  • Predicting pregnancy outcome after in vitro fertilization and embryo transfer using estradiol, progesterone, and human chorionic gonadotropin beta-subunit.

    abstract::Two hundred twenty consecutive in vitro fertilization (IVF) conception cycles were studied prospectively in order to examine the predictive value of serum human chorionic gonadotropin beta-subunit (beta-hCG), estradiol (E2), and progesterone (P) in predicting pregnancy outcome between 2 and 4 weeks after oocyte collec...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Yamashita T,Okamoto S,Thomas A,MacLachlan V,Healy DL

    更新日期:1989-02-01 00:00:00

  • A case of a methotrexate-resistant ectopic pregnancy in which dactinomycin was effective as a second-line chemotherapy.

    abstract:OBJECTIVE:To report a case of a methotrexate (MTX)-resistant heterotopic interstitial pregnancy in which dactinomycin was necessary as a second-line chemotherapy. DESIGN:Case report. SETTING:Medical college-affiliated hospital. PATIENT(S):A 39-year-old woman with vaginal bleeding after the transfer of two thawed 8-c...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Fujioka S,Yamashita Y,Kawabe S,Kamegai H,Terai Y,Ohmichi M

    更新日期:2009-03-01 00:00:00

  • Follicular fluid levels of insulin-like growth factor I, insulin-like growth factor binding protein I, and ovarian steroids collected during ovum pick-up.

    abstract:OBJECTIVE:To evaluate interrelationships between levels of insulin-like growth factor I (IGF-I), insulin-like growth factor binding protein (IGFBP-I), and ovarian steroids in late preovulatory follicular fluid (FF) after pituitary desensitization with GnRH agonist and controlled ovarian hyperstimulation. DESIGN:Follic...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Chang SY,Hsieh KC,Wang HS,Soong YK

    更新日期:1994-12-01 00:00:00