Extraction of cholesterol with methyl-beta-cyclodextrin perturbs formation of clathrin-coated endocytic vesicles.

abstract：:Motile cells such as bacteria, amoebae, and fibroblasts display a continual level of energy-consuming reactions involving the cytoskeleton and signal pathways, regardless of whether or not they are actually migrating. I draw parallels between these "silent signals" and the intrinsic activity of the human brain, especi...

journal_title：Molecular biology of the cell

authors： Bray D

更新日期：2014-03-01 00:00:00

abstract：:Significant increase in JNK, c-Jun, and Cdk5 activities are reported in Alzheimer's disease (AD). Inhibition of c-Jun prevents neuronal cell death in in vivo AD models, highlighting it as a major JNK effector. Both JNK and Cdk5 promote neurodegeneration upon deregulation; however, Cdk5 has not been mechanistically lin...

journal_title：Molecular biology of the cell

authors： Sun KH,Lee HG,Smith MA,Shah K

更新日期：2009-11-01 00:00:00

abstract：:How recycling receptors are segregated from down-regulated receptors in the endosome is unknown. In previous studies, we demonstrated that substitutions in the transferrin receptor (TR) transmembrane domain (TM) convert the protein from an efficiently recycling receptor to one that is rapidly down regulated. In this s...

journal_title：Molecular biology of the cell

authors： Zaliauskiene L,Kang S,Brouillette CG,Lebowitz J,Arani RB,Collawn JF

更新日期：2000-08-01 00:00:00

abstract：:During mating, Saccharomyces cerevisiae cells must degrade the intervening cell wall to allow fusion of the partners. Because improper timing or location of cell wall degradation would cause lysis, the initiation of cell fusion must be highly regulated. Here, we find that yeast cell fusion is negatively regulated by c...

journal_title：Molecular biology of the cell

authors： Hall AE,Rose MD

更新日期：2019-02-15 00:00:00

abstract：:Secretory proteins are exported from the endoplasmic reticulum in COPII vesicles. SNARE proteins-core machinery for membrane fusion-are incorporated into COPII vesicles by direct interaction with Sec24. Here we report a novel mechanism for sorting of the ER-Golgi Q-SNAREs into COPII vesicles. Different mammalian Sec24...

journal_title：Molecular biology of the cell

authors： Adolf F,Rhiel M,Reckmann I,Wieland FT

更新日期：2016-09-01 00:00:00

abstract：:Alternative pre-mRNA splicing patterns can change an extracellular stimulus, but the signaling pathways leading to these changes are still poorly characterized. Here, we describe a tyrosine-phosphorylated nuclear protein, YT521-B, and show that it interacts with the nuclear transcriptosomal component scaffold attachme...

journal_title：Molecular biology of the cell

authors： Hartmann AM,Nayler O,Schwaiger FW,Obermeier A,Stamm S

更新日期：1999-11-01 00:00:00

abstract：:Yeast that naturally exhaust the glucose from their environment differentiate into three distinct cell types distinguishable by flow cytometry. Among these is a quiescent (Q) population, which is so named because of its uniform but readily reversed G1 arrest, its fortified cell walls, heat tolerance, and longevity. Da...

journal_title：Molecular biology of the cell

authors： Li L,Miles S,Melville Z,Prasad A,Bradley G,Breeden LL

更新日期：2013-12-01 00:00:00

abstract：:The aim of binding assays is to measure interactions between two molecules, such as a protein binding another protein, a small molecule, or a nucleic acid. Hard work is required to prepare reagents, but flaws in the design of many binding experiments limit the information obtained. In particular many experiments fail ...

journal_title：Molecular biology of the cell

authors： Pollard TD

更新日期：2010-12-01 00:00:00

abstract：:The kinetochore is a crucial structure for faithful chromosome segregation during mitosis and is formed in the centromeric region of each chromosome. The 16-subunit protein complex known as the constitutive centromere-associated network (CCAN) forms the foundation for kinetochore assembly on the centromeric chromatin....

journal_title：Molecular biology of the cell

authors： Nagpal H,Hori T,Furukawa A,Sugase K,Osakabe A,Kurumizaka H,Fukagawa T

更新日期：2015-11-01 00:00:00

abstract：:Two cDNAs encoding casein kinase-1 have been isolated from a yeast cDNA library and termed CKI1 and CKI2. Each clone encodes a protein of approximately 62,000 Da containing a highly conserved protein kinase domain surrounded by variable amino- and carboxy-terminal domains. The proteins also contain two conserved carbo...

journal_title：Molecular biology of the cell

authors： Wang PC,Vancura A,Mitcheson TG,Kuret J

更新日期：1992-03-01 00:00:00

abstract：:The structural maintenance of chromosomes (SMC) protein encoded by the fission yeast rad18 gene is involved in several DNA repair processes and has an essential function in DNA replication and mitotic control. It has a heterodimeric partner SMC protein, Spr18, with which it forms the core of a multiprotein complex. We...

journal_title：Molecular biology of the cell

authors： Taylor EM,Moghraby JS,Lees JH,Smit B,Moens PB,Lehmann AR

更新日期：2001-06-01 00:00:00

abstract：:Wounding of endothelial cells is associated with altered direct intercellular communication. To determine whether gap junctional communication participates to the wound repair process, we have compared connexin (Cx) expression, cell-to-cell coupling and kinetics of wound repair in monolayer cultures of PymT-transforme...

journal_title：Molecular biology of the cell

authors： Kwak BR,Pepper MS,Gros DB,Meda P

更新日期：2001-04-01 00:00:00

abstract：:Cellular levels of the versatile second messenger cyclic (c)AMP are regulated by the antagonistic actions of the canonical G protein → adenylyl cyclase pathway that is initiated by G-protein-coupled receptors (GPCRs) and attenuated by phosphodiesterases (PDEs). Dysregulated cAMP signaling drives many diseases; for exa...

journal_title：Molecular biology of the cell

authors： Getz M,Swanson L,Sahoo D,Ghosh P,Rangamani P

更新日期：2019-06-15 00:00:00

abstract：:The inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular calcium channel involved in coupling cell membrane receptors to calcium signal transduction pathways within cells including endocrine cells. Several isoforms (I, II, and III) of IP3Rs have been identified, which are encoded by separate genes, and are...

journal_title：Molecular biology of the cell

authors： Nucifora FC Jr,Sharp AH,Milgram SL,Ross CA

更新日期：1996-06-01 00:00:00

abstract：:When Pichia pastoris adapts from methanol to glucose growth, peroxisomes are rapidly sequestered and degraded within the vacuole by micropexophagy. During micropexophagy, sequestering membranes arise from the vacuole to engulf the peroxisomes. Fusion of the sequestering membranes and incorporation of the peroxisomes i...

journal_title：Molecular biology of the cell

authors： Chang T,Schroder LA,Thomson JM,Klocman AS,Tomasini AJ,Strømhaug PE,Dunn WA Jr

更新日期：2005-10-01 00:00:00

abstract：:Dynamic microtubule ends exert pulling and pushing forces on intracellular membranes and organelles. However, the mechanical linkage of microtubule tips to their cargoes is poorly understood. CENP-F is a nonmotor microtubule-binding protein that participates in microtubule binding at kinetochores and in the mitotic re...

journal_title：Molecular biology of the cell

authors： Kanfer G,Peterka M,Arzhanik VK,Drobyshev AL,Ataullakhanov FI,Volkov VA,Kornmann B

更新日期：2017-09-01 00:00:00

abstract：:We have investigated the role of myosin in cytokinesis in Dictyostelium cells by examining cells under both adhesive and nonadhesive conditions. On an adhesive surface, both wild-type and myosin-null cells undergo the normal processes of mitotic rounding, cell elongation, polar ruffling, furrow ingression, and separat...

journal_title：Molecular biology of the cell

authors： Zang JH,Cavet G,Sabry JH,Wagner P,Moores SL,Spudich JA

更新日期：1997-12-01 00:00:00

abstract：:Colony-stimulating factor-1 (CSF-1) induces expression of immediate early gene, such as c-myc and c-fos and delayed early genes such as D-type cyclins (D1 and D2), whose products play essential roles in the G1 to S phase transition of the cell cycle. Little is known, however, about the cytoplasmic signal transduction ...

journal_title：Molecular biology of the cell

authors： Dey A,She H,Kim L,Boruch A,Guris DL,Carlberg K,Sebti SM,Woodley DT,Imamoto A,Li W

更新日期：2000-11-01 00:00:00

abstract：:Cell morphogenesis is a complex process that relies on a diverse array of proteins and pathways. We have identified a transglutaminase-like protein (Cyk3p) that functions in fission yeast morphogenesis. The phenotype of a cyk3 knockout strain indicates a primary role for Cyk3p in cytokinesis. Correspondingly, Cyk3p lo...

journal_title：Molecular biology of the cell

authors： Pollard LW,Onishi M,Pringle JR,Lord M

更新日期：2012-07-01 00:00:00

abstract：:The juvenile form of neuronal ceroid lipofuscinoses (JNCLs), or Batten disease, results from mutations in the CLN3 gene, and it is characterized by the accumulation of lipopigments in the lysosomes of several cell types and by extensive neuronal death. We report that the yeast model for JNCL (btn1-Delta) that lacks BT...

journal_title：Molecular biology of the cell

authors： Osório NS,Carvalho A,Almeida AJ,Padilla-Lopez S,Leão C,Laranjinha J,Ludovico P,Pearce DA,Rodrigues F

更新日期：2007-07-01 00:00:00

abstract：:The AP-1 transcription factor JunD is highly expressed in intestinal epithelial cells, but its exact role in maintaining the integrity of intestinal epithelial barrier remains unknown. The tight junction (TJ) protein zonula occludens (ZO)-1 links the intracellular domain of TJ-transmembrane proteins occludin, claudins...

journal_title：Molecular biology of the cell

authors： Chen J,Xiao L,Rao JN,Zou T,Liu L,Bellavance E,Gorospe M,Wang JY

更新日期：2008-09-01 00:00:00

abstract：:Biogenesis of respiratory chain complexes depends on the expression of mitochondrial-encoded subunits. Their synthesis occurs on membrane-associated ribosomes and is probably coupled to their membrane insertion. Defects in expression of mitochondrial translation products are among the major causes of mitochondrial dis...

journal_title：Molecular biology of the cell

authors： Bauerschmitt H,Mick DU,Deckers M,Vollmer C,Funes S,Kehrein K,Ott M,Rehling P,Herrmann JM

更新日期：2010-06-15 00:00:00

abstract：:The BNIP-2 and Cdc42GAP homology (BCH) domain is a novel regulator for Rho GTPases, but its impact on p50-Rho GTPase-activating protein (p50RhoGAP or Cdc42GAP) in cells remains elusive. Here we show that deletion of the BCH domain from p50RhoGAP enhanced its GAP activity and caused drastic cell rounding. Introducing c...

journal_title：Molecular biology of the cell

authors： Zhou YT,Chew LL,Lin SC,Low BC

更新日期：2010-09-15 00:00:00

abstract：:During their migration to the periphery, cranial neural crest cells (NCCs) are repulsed by an ErbB4-dependent cue(s) in the mesenchyme adjoining rhombomeres (r) 3 and 5, which are segmented hindbrain neuromeres. ErbB4 has many ligands, but which ligand functions in the above system has not yet been clearly determined....

journal_title：Molecular biology of the cell

authors： Hoshino H,Uchida T,Otsuki T,Kawamoto S,Okubo K,Takeichi M,Chisaka O

更新日期：2007-04-01 00:00:00

abstract：:The key molecular regulatory mechanisms that govern and coordinate the molecular alterations that underpin the process of human labor remain incompletely understood although enhanced intrauterine prostaglandin production is known to be requisite. Studies from cancer tissues have indicated that at least one key enzyme ...

journal_title：Molecular biology of the cell

authors： Mitchell MD

更新日期：2006-01-01 00:00:00

abstract：:Cell-cell fusion is an intriguing differentiation process, essential for placental development and maturation. A proteomic approach identified a cytoplasmic protein, calponin 3 (CNN3), related to the fusion of BeWo choriocarcinoma cells. CNN3 was expressed in cytotrophoblasts in human placenta. CNN3 gene knockdown pro...

journal_title：Molecular biology of the cell

authors： Shibukawa Y,Yamazaki N,Kumasawa K,Daimon E,Tajiri M,Okada Y,Ikawa M,Wada Y

更新日期：2010-11-15 00:00:00

abstract：:Neural cell adhesion molecules (CAMs) are important players during neurogenesis and neurite outgrowth as well as axonal fasciculation and pathfinding. Some of these developmental processes entail the activation of cellular signaling cascades. Pharmacological and genetic evidence indicates that the neurite outgrowth-pr...

journal_title：Molecular biology of the cell

authors： Islam R,Kristiansen LV,Romani S,Garcia-Alonso L,Hortsch M

更新日期：2004-04-01 00:00:00

abstract：:Myosin- and Rab-interacting protein (MyRIP), which belongs to the protein kinase A (PKA)-anchoring family, is implicated in hormone secretion. However, its mechanism of action is not fully elucidated. Here we investigate the role of MyRIP in myosin Va (MyoVa)-dependent secretory granule (SG) transport and secretion in...

journal_title：Molecular biology of the cell

authors： Brozzi F,Lajus S,Diraison F,Rajatileka S,Hayward K,Regazzi R,Molnár E,Váradi A

更新日期：2012-11-01 00:00:00

abstract：:The RNA-binding protein HuD binds to a regulatory element in the 3' untranslated region (3' UTR) of the GAP-43 mRNA. To investigate the functional significance of this interaction, we generated PC12 cell lines in which HuD levels were controlled by transfection with either antisense (pDuH) or sense (pcHuD) constructs....

journal_title：Molecular biology of the cell

authors： Mobarak CD,Anderson KD,Morin M,Beckel-Mitchener A,Rogers SL,Furneaux H,King P,Perrone-Bizzozero NI

更新日期：2000-09-01 00:00:00

abstract：:Focal adhesion kinase (FAK) is a cytoplasmic tyrosine kinase protein localized to regions called focal adhesions, which are contact points between cells and the extracellular matrix. FAK protein acts as a scaffold to transfer adhesion-dependent and growth factor signals into the cell. Increased FAK expression is linke...

journal_title：Molecular biology of the cell

authors： Fonar Y,Gutkovich YE,Root H,Malyarova A,Aamar E,Golubovskaya VM,Elias S,Elkouby YM,Frank D

更新日期：2011-07-01 00:00:00