Abstract:
:Dynamic microtubule ends exert pulling and pushing forces on intracellular membranes and organelles. However, the mechanical linkage of microtubule tips to their cargoes is poorly understood. CENP-F is a nonmotor microtubule-binding protein that participates in microtubule binding at kinetochores and in the mitotic redistribution of the mitochondrial network. CENP-F-driven mitochondrial transport is linked to growing microtubule tips, but the underlying molecular mechanisms are unknown. Here we show that CENP-F tracks growing microtubule ends in living cells. In vitro reconstitution demonstrates that microtubule tips can transport mitochondria and CENP-F-coated artificial cargoes over micrometer-long distances during both growing and shrinking phases. Based on these and previous observations, we suggest that CENP-F might act as a transporter of mitochondria and other cellular cargoes by attaching them to dynamic microtubule ends during both polymerization and depolymerization of tubulin.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Kanfer G,Peterka M,Arzhanik VK,Drobyshev AL,Ataullakhanov FI,Volkov VA,Kornmann Bdoi
10.1091/mbc.E16-11-0756subject
Has Abstractpub_date
2017-09-01 00:00:00pages
2400-2409issue
18eissn
1059-1524issn
1939-4586pii
mbc.E16-11-0756journal_volume
28pub_type
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