当前位置： SCI文献检索 > MOLECULAR AND CELLULAR ENDOCRINOLOGY期刊下所有文献 > Different bioactivities of human thyrotropin receptors with different signal peptides.

Different bioactivities of human thyrotropin receptors with different signal peptides.

Abstract：

:For investigation of the mechanism and pathogenesis of Graves' disease, availability of a large amount of functional human thyrotropin receptor (TSHR) capable of recognition by Graves' autoantibodies is essential. Many attempts have been made to produce the extracellular domain of TSH receptor (TSHRE) in a baculovirus expression system. However, the receptor is expressed as an insoluble form and the refolded protein is often not recognized by the autoantibodies. In this study, we found that the TSHRE expressed with its own signal peptide (VL3-RE) in insect cells is retained inside of the cells and found in both soluble and insoluble fractions in equal proportion. The signal peptide is not removed. The receptor in the soluble fraction is not recognized by either TSH or Graves' autoantibodies. The TSHRE with an insect-specific mellitin signal peptide (Mel-RE) is also retained inside of the cell and found in both the soluble and insoluble fractions in equal proportion. However, the signal peptide is removed and the receptor is recognized by the Graves' autoantibodies but not by TSH. Also, the amount of Mel-RE expressed was 5-10-fold higher than VL3-RE. The two receptor preparations apparently have the same degree of glycosylation as evidenced by the same increased mass (approximately 15 kDa) due to glycosylation. However, the two receptors have different affinity for an anion-exchange resin and different pI. Deglycosylated receptors have the same pI. This suggests that the composition of sugars may be different. Taken together, the results suggest that the two receptors are modified and folded differently by different pathways due to the presence of different signal peptides. Use of an insect-specific signal peptide is recommended for expression of TSHR that is recognized by Graves' autoantibodies in a baculovirus system.

journal_name

Mol Cell Endocrinol

journal_title

Molecular and cellular endocrinology

authors

Park JY,Lee J,Cho BY,Chae CB

doi

10.1016/s0303-7207(98)00209-3

keywords:

subject

Has Abstract

pub_date

1999-01-25 00:00:00

pages

133-42

issue

1-2

eissn

0303-7207

issn

1872-8057

journal_volume

147

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR ENDOCRINOLOGY文献大全
  • Conjugated linoleic acid inhibits glucose metabolism, leptin and adiponectin secretion in primary cultured rat adipocytes.

    abstract：:Conjugated linoleic acid (CLA) supplementation has been reported to induce insulin resistance in animals and humans, however, the underlying mechanisms remain unclear. The aim of this study was to examine the direct effects of CLA on leptin and adiponectin secretion, two hormones with actions known to influence insuli...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2007.01.013

    authors： Pérez-Matute P,Marti A,Martínez JA,Fernández-Otero MP,Stanhope KL,Havel PJ,Moreno-Aliaga MJ

    更新日期：2007-03-30 00:00:00

  • A review on the mechanism of iodide metabolic dysfunction in differentiated thyroid cancer.

    abstract：:The incidence of differentiated thyroid cancer (DTC) has been increasing rapidly worldwide, and the risk factors remain unclear. With the growing number of patients with DTC, the related issues have been gradually highlighted. 131Iodide (131I) is an important treatment for DTC and has the potential to reduce the risk ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2018.09.002

    authors： Zhao Y,Zhong L,Yi H

    更新日期：2019-01-05 00:00:00

  • Morphological evidence of lysosomal uptake of high-density lipoproteins by rat adrenocortical cells in vitro.

    abstract：:The high-density lipoprotein (HDL) pathway in rat adrenocortical cells was studied at the electron microscopic level in vitro via colloidal gold labelling. Steroid hormone assays were performed to confirm that the cells remained intact, viable, responsive to ACTH under the applied conditions, and to reveal the steroid...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(86)90062-6

    authors： Tóth IE,Szabó D,Bácsy E,Szalay KS,Hesz A,Szollár LG

    更新日期：1986-02-01 00:00:00

  • Expression of two isoforms of the human growth hormone receptor in normal liver and hepatocarcinoma.

    abstract：:In man, two isoforms of growth hormone receptor (GHR) have been reported. The first, hGHR corresponds to the original form described by Leung et al. (J. Biol. Chem. 264 (1987) 9905-9910). The second, hGHRd3, missing 22 N-terminal amino acids is encoded by an mRNA derived from alternative splicing and deletion of the t...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(94)90064-7

    authors： Esposito N,Paterlini P,Kelly PA,Postel-Vinay MC,Finidori J

    更新日期：1994-07-01 00:00:00

  • Metformin regulates stromal-epithelial cells communication via Wnt2/β-catenin signaling in endometriosis.

    abstract：:In previous studies, we found that endometriotic stromal cells lose the ability to regulate cell survival signaling in endometriotic epithelial cells. Here, we invested the effect of Metformin on the stromal-epithelial cells crosstalk in endometriosis and explored the pathway that might be involved. We found that ecto...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2015.06.011

    authors： Zhang H,Xue J,Li M,Zhao X,Wei D,Li C

    更新日期：2015-09-15 00:00:00

  • MicroRNA-126 upregulation, induced by training, plays a role in controlling microcirculation in dexamethasone treated rats.

    abstract：:Microcirculation maintenance is associated with microRNAs. Nevertheless, the role of microRNAs induced by training in preventing dexamethasone (DEX)-induced microvascular rarefaction remains unknown. The study aim was to investigate if training-induced microRNAs are able to improve microcirculation proteins and preven...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2020.110732

    authors： Herrera NA,Duchatsch F,Tardelli LP,Dionísio TJ,Shinohara AL,Santos CF,Amaral SL

    更新日期：2020-04-05 00:00:00

  • Secretogranin I (chromogranin B) mRNA accumulation is hormonally regulated in GH3B6 rat pituitary tumor cells.

    abstract：:Secretogranin I (SgI; chromogranin B) belongs to a class of acidic tyrosine-sulfated secretory proteins believed to play a role in the secretory process of endocrine cells. Our aim here was to compare the levels of SgI mRNA to that of prolactin (PRL) and growth hormone (GH), using rat pituitary cell lines. As far as t...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(91)90141-e

    authors： Laverrière JN,Richard JL,Morin A,Buisson N,Tixier-Vidal A,Huttner WB,Gourdji D

    更新日期：1991-09-01 00:00:00

  • In vitro characterization and endocrine regulation of cholesterol and phospholipid transport in the mammary gland.

    abstract：:Cell-based studies previously showed that the ATP-binding cassette transporter A1 (ABCA1) transfers cholesterol across mammary epithelial cells (MEC). Data for phospholipid transport are lacking, and it is unclear from which cellular source the transported cholesterol stems, whether this transport activates signaling ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2016.10.016

    authors： Ontsouka CE,Huang X,Aliyev E,Albrecht C

    更新日期：2017-01-05 00:00:00

  • Pinopodes: Recent advancements, current perspectives, and future directions.

    abstract：:Successful embryo implantation is a complex and highly regulated process involving precise synchronization between the fetal-derived trophoblast cells and maternal uterine luminal epithelium. Multiple endocrine-driven factors are important for controlling the timely receptivity of the uterus, and this complexity under...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2019.110644

    authors： Quinn KE,Matson BC,Wetendorf M,Caron KM

    更新日期：2020-02-05 00:00:00

  • Effect of thyroid status on the development of the different molecular forms of Na+,K+-ATPase in rat brain.

    abstract：:The effect of thyroid status on the postnatal development of the two molecular forms of Na+,K+-ATPase, distinguished kinetically on the basis of their ouabain sensitivity, was examined in rat brain. Hypothyroidism induced by PTU from day 1 postnatally significantly reduced the Na+,K+-ATPase activity in cerebellum (22-...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(85)90170-4

    authors： Atterwill CK,Reid J,Athayde CM

    更新日期：1985-05-01 00:00:00

  • MAP kinase phosphatase 3 inhibits brown adipocyte differentiation via regulation of Erk phosphorylation.

    abstract：:Brown fat has been highlight as a new therapeutic target for treatment of obesity and diabetes. However, molecular mechanism underlying brown adipogenesis are not fully understood. Here, we identified that MAP kinase phosphatase 3 (MKP3) has a novel role as regulator of brown adipocyte differentiation. The expression ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2015.08.023

    authors： Kim WK,Oh KJ,Choi HR,Park A,Han BS,Chi SW,Kim SJ,Bae KH,Lee SC

    更新日期：2015-11-15 00:00:00

  • The sexual dimorphism of obesity.

    abstract：:The NIH has recently highlighted the importance of sexual dimorphisms and has mandated inclusion of both sexes in clinical trials and basic research. In this review we highlight new and novel ways sex hormones influence body adiposity and the metabolic syndrome. Understanding how and why metabolic processes differ by ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2014.11.029

    authors： Palmer BF,Clegg DJ

    更新日期：2015-02-15 00:00:00

  • Glycosylation variants of human TSH selectively activate signal transduction pathways.

    abstract：:The oligosaccharide chains of pituitary glycoprotein hormones such as human thyroid-stimulating hormone (hTSH) have been shown to be important in biosynthesis, subunit association, secretion and bioactivity. However, the exact biological significance of these glycosylation variants (isoforms) remains controversial. Th...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(97)00136-6

    authors： Schaaf L,Leiprecht A,Saji M,Hübner U,Usadel KH,Kohn LD

    更新日期：1997-09-19 00:00:00

  • 11Beta-hydroxysteroid dehydrogenase expression in first trimester human trophoblasts.

    abstract：:This study evaluated the levels and the enzymatic characteristics of 11beta-hydroxysteroid dehydrogenase activity (11beta-HSD) of chorionic villi isolated from first trimester human placenta. The results demonstrated a predominant expression of the NAD-dependent dehydrogenase isoform (11beta-HSD2) over the NADP-depend...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(98)00103-8

    authors： Arcuri F,Sestini S,Paulesu L,Bracci L,Carducci A,Manzoni F,Cardone C,Cintorino M

    更新日期：1998-06-25 00:00:00

  • Bypassing natural sperm selection during fertilization: the azh mutant offspring experience and the alternative of spermiogenesis in vitro.

    abstract：:Molecular aspects of spermiogenesis can be studied using mouse mutants and spermatids developed in vitro. The azh/azh mutant is an attractive model system because structural abnormalities in the sperm head and the ectopic position of the manchette are associated with tail bending and looping. Spermatids, developing an...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/s0303-7207(01)00692-x

    authors： Kierszenbaum AL,Tres LL

    更新日期：2002-02-22 00:00:00

  • Somatostatin analogs regulate tumor corticotrophs growth by reducing ERK1/2 activity.

    abstract：:Pasireotide has been associated with tumor shrinkage in patients with Cushing's disease subjected to long term treatment. However, to date the implicated molecular mechanisms are poorly elucidated. Here, we tested pasireotide-mediated cytostatic and cytotoxic effects in ACTH-secreting primary tumor cultures and murine...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2018.12.022

    authors： Treppiedi D,Giardino E,Catalano R,Mangili F,Vercesi P,Sala E,Locatelli M,Arosio M,Spada A,Mantovani G,Peverelli E

    更新日期：2019-03-01 00:00:00

  • Molecular cloning and endometrial expression of porcine high density lipoprotein receptor SR-BI during the estrous cycle and early pregnancy.

    abstract：:During rapid development of the fetus, levels of high density lipoprotein (HDL) are elevated in pregnant women. The receptor for HDL, scavenger receptor class B type I (SR-BI), mediates selective cholesteryl ester uptake and is highly expressed in the human placenta. Because of the rapid growth of uterus during early ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2004.04.008

    authors： Kim JG,Vallet JL,Nonneman D,Christenson RK

    更新日期：2004-07-30 00:00:00

  • Freezing of human ovarian tissue--not the oocytes but the granulosa is the problem.

    abstract：:The freezing of human ovarian tissue may be the key for restoring fertility after systemic therapy of cancer. In contrast to others we investigated the survival rate of whole follicles, and had a special look at the granulosa cells. Ovarian tissue was collected laparoscopically (n = 10) and divided into equal parts fo...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(00)00362-2

    authors： Siebzehnrübl E,Kohl J,Dittrich R,Wildt L

    更新日期：2000-11-27 00:00:00

  • Melanocortin 4 receptor is a transcriptional target of nescient helix-loop-helix-2.

    abstract：:Melanocortin 4 receptor (Mc4r/MC4R) is a G-Protein coupled receptor that is expressed in the hypothalamus and implicated in body weight control. Mutations in MC4R are the most frequent cause of monogenetic forms of human obesity. Despite its importance, the MC4R signaling pathways and transcriptional regulation underl...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2011.05.022

    authors： Wankhade UD,Good DJ

    更新日期：2011-07-20 00:00:00

  • Insights into sperm cell motility signaling through sNHE and the CatSpers.

    abstract：:Successful natural reproduction normally requires vigorously motile spermatozoa. Using a signal peptide trapping strategy, we identified two new genes, a putative sperm Na+/H+ exchanger (sNHE) and the putative cation channel CatSper2, with unique and essential roles in sperm motility. Disruption of the sNHE or CatSper...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2005.12.031

    authors： Quill TA,Wang D,Garbers DL

    更新日期：2006-05-16 00:00:00

  • FOXO3a governs early and late apoptotic endothelial programs during elevated glucose through mitochondrial and caspase signaling.

    abstract：:Mechanisms that preserve endothelial cell (EC) integrity remain elusive, but are critical for new strategies directed against endocrine disorders such as diabetes mellitus (DM). Here we demonstrate in primary cerebral ECs with a clinically relevant model of elevated d-glucose that Akt1 and the post-translational modif...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2010.02.037

    authors： Hou J,Chong ZZ,Shang YC,Maiese K

    更新日期：2010-06-10 00:00:00

  • Structure and distribution of rat menin mRNA.

    abstract：:Menin is a protein product of a tumor suppressor gene MEN1, mutations of which are responsible for multiple endocrine neoplasia type 1, an autosomal dominant familial cancer syndrome. We isolated rat menin cDNA clones from a fetal rat brain cDNA library. We also determined the nucleotide sequence of the protein coding...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(99)00150-1

    authors： Maruyama K,Tsukada T,Hosono T,Ohkura N,Kishi M,Honda M,Nara-Ashizawa N,Nagasaki K,Yamaguchi K

    更新日期：1999-10-25 00:00:00

  • Pongamol from Pongamia pinnata stimulates glucose uptake by increasing surface GLUT4 level in skeletal muscle cells.

    abstract：:Skeletal muscle is the major site of postprandial glucose disposal and augmenting glucose uptake into this tissue may attenuate insulin resistance that precedes type 2 diabetes mellitus. Here, we investigated the effect of pongamol, an identified lead molecule from the fruits of Pongamia pinnata, on glucose uptake and...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2011.03.023

    authors： Tamrakar AK,Jaiswal N,Yadav PP,Maurya R,Srivastava AK

    更新日期：2011-06-06 00:00:00

  • Adrenal zonation: clues from 11beta-hydroxylase and aldosterone synthase.

    abstract：:Aldosterone and cortisol are the major mineralocorticoid and glucocorticoid produced by the human adrenal. Circulating levels of angiotensin II and potassium control the adrenal production of aldosterone, while the production of cortisol is controlled mainly by adrenocorticotropin. The capacity of the adrenal cortex t...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/s0303-7207(99)00051-9

    authors： Rainey WE

    更新日期：1999-05-25 00:00:00

  • Inhibition of FSH action on granulosa cells by low molecular weight components of follicular fluid.

    abstract：:Immature female rats were injected with a single dose (10 IU) of pregnant mare serum gonadotropin to induce growth and maturation of ovarian follicles. Using such ovaries as a model, we tested the effects of low molecular weight subfractions of charcoal-absorbed bovine follicular fluid (FF-c) on (a) radioiodinated hum...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(82)90086-7

    authors： Fletcher PW,Dias JA,Sanzo MA,Reichert LE Jr

    更新日期：1982-03-01 00:00:00

  • Estrogen receptor beta signals to inhibition of cardiac fibrosis.

    abstract：:Cardiac fibrosis evolves from the cardiac hypertrophic state. In this respect, estrogen and estrogen receptor beta (ERβ) inhibit the effects of cardiac hypertrophic peptides that also stimulate fibrosis. Here we determine details of the anti-fibrotic functions of ERβ. In acutely isolated rat cardiac fibroblasts. E2 or...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2016.06.018

    authors： Pedram A,Razandi M,Narayanan R,Levin ER

    更新日期：2016-10-15 00:00:00

  • Glucocorticoid receptor knock down reveals a similar apoptotic threshold but differing gene regulation patterns in T-cell and pre-B-cell acute lymphoblastic leukemia.

    abstract：:Glucocorticoids (GCs) are used in combination therapy for treating acute lymphoblastic leukemia (ALL). In T-cell (CEM-C7) and pre-B-cell (697) ALL cell lines, dexamethasone (Dex) treatment causes an auto-upregulation of glucocorticoid receptor (GR) mRNA transcripts and protein. We hypothesized that there is a threshol...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2010.02.014

    authors： Schwartz JR,Sarvaiya PJ,Vedeckis WV

    更新日期：2010-05-14 00:00:00

  • Regulated expression of vasopressin gene by cAMP and phorbol ester in primary rat fetal hypothalamic cultures.

    abstract：:Using dispersed cultures of fetal rat hypothalami, we studied the effects of forskolin and the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA), activators of protein kinase A and C, respectively, upon vasopressin (VP) secretion, VP mRNA expression and VP mRNA poly(A) tail length. Forskolin stimulated the VP m...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(92)90172-3

    authors： Emanuel RL,Girard DM,Thull DL,Majzoub JA

    更新日期：1992-07-01 00:00:00

  • Isolation, characterisation and tissue localisation of an N-terminal-truncated variant of fibroblast growth factor.

    abstract：:An additional form of basic fibroblast growth factor (FGF), possessing full biological activity, has been isolated from bovine pituitary extracts. This polypeptide mitogen exhibits an apparent molecular weight of 17,500 by SDS-PAGE and lacks the first four N-terminal amino acid residues (Pro-Ala-Leu-Pro) of a previous...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(87)90028-1

    authors： Bertolini J,Hearn MT

    更新日期：1987-06-01 00:00:00

  • The androgen-binding protein gene is expressed in CD1 mouse testis.

    abstract：:Androgen-binding protein (ABP) is a testicular Sertoli cell secretory protein that acts as a carrier of androgen in the male reproductive tract. ABP has been characterized from a wide range of animal species, including man, rabbit and rat. However, it has been widely accepted that mice do not produce testicular ABP. W...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(89)90084-1

    authors： Wang YM,Sullivan PM,Petrusz P,Yarbrough W,Joseph DR

    更新日期：1989-05-01 00:00:00