当前位置： SCI文献检索 > Journal of Neuroinflammation期刊下所有文献 > Functional characteristics of Th1, Th17, and ex-Th17 cells in EAE revealed by intravital two-photon microscopy.

Functional characteristics of Th1, Th17, and ex-Th17 cells in EAE revealed by intravital two-photon microscopy.

Abstract：

BACKGROUND:T helper (Th) 17 cells are a highly plastic subset of T cells, which in the context of neuroinflammation, are able to acquire pathogenic features originally attributed to Th1 cells (resulting in so called ex-Th17 cells). Thus, a strict separation between the two T cell subsets in the context of experimental autoimmune encephalomyelitis (EAE) is difficult. High variability in culture and EAE induction protocols contributed to previous conflicting results concerning the differential contribution of Th1 and Th17 cells in EAE. Here, we systematically evaluate the role of different T cell differentiation and transfer protocols for EAE disease development and investigate the functional dynamics of encephalitogenic T cells directly within the inflamed central nervous system (CNS) tissue. METHODS:We compiled the currently used EAE induction protocols reported in literature and investigated the influence of the different Th1 and Th17 differentiation protocols as well as EAE induction protocols on the EAE disease course. Moreover, we assessed the cytokine profile and functional dynamics of both encephalitogenic Th1 and Th17 cells in the inflamed CNS using flow cytometry and intravital two-photon laser scanning microscopy. Lastly, we used astrocyte culture and adoptive transfer EAE to evaluate the impact of Th1 and Th17 cells on astrocyte adhesion molecule expression in vitro and in vivo. RESULTS:We show that EAE courses are highly dependent on in vitro differentiation and transfer protocols. Moreover, using genetically encoded reporter mice (B6.IL17A-EGFP.acRFP x 2d2/2d2.RFP), we show that the motility of interferon (IFN)γ-producing ex-Th17 cells more closely resembles Th1 cells than Th17 cells in transfer EAE. Mechanistically, IFNγ-producing Th1 cells selectively induce the expression of cellular adhesion molecules I-CAM1 while Th1 as well as ex-Th17 induce V-CAM1 on astrocytes. CONCLUSIONS:The behavior of ex-Th17 cells in EAE lesions in vivo resembles Th1 rather than Th17 cells, underlining that their change in cytokine production is associated with functional phenotype alterations of these cells.

journal_name

J Neuroinflammation

journal_title

Journal of neuroinflammation

authors

Loos J,Schmaul S,Noll TM,Paterka M,Schillner M,Löffel JT,Zipp F,Bittner S

doi

10.1186/s12974-020-02021-x

subject

Has Abstract

pub_date

2020-11-26 00:00:00

pages

357

issue

1

issn

1742-2094

pii

10.1186/s12974-020-02021-x

journal_volume

17

pub_type

杂志文章

相关文献

Journal of Neuroinflammation文献大全
  • Role of docosahexaenoic acid in the modulation of glial cells in Alzheimer's disease.

    abstract：:Docosahexaenoic acid (DHA) is an omega-3 (ω-3) long-chain polyunsaturated fatty acid (LCPUFA) relevant for brain function. It has largely been explored as a potential candidate to treat Alzheimer's disease (AD). Clinical evidence favors a role for DHA in the improvement of cognition in very early stages of the AD. In ...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章,评审

    doi：10.1186/s12974-016-0525-7

    authors： Heras-Sandoval D,Pedraza-Chaverri J,Pérez-Rojas JM

    更新日期：2016-03-10 00:00:00

  • [18F]FSPG-PET reveals increased cystine/glutamate antiporter (xc-) activity in a mouse model of multiple sclerosis.

    abstract：BACKGROUND:The cystine/glutamate antiporter (xc-) has been implicated in several neurological disorders and, specifically, in multiple sclerosis (MS) as a mediator of glutamate excitotoxicity and proinflammatory immune responses. We aimed to evaluate an xc-specific positron emission tomography (PET) radiotracer, (4S)-4...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-018-1080-1

    authors： Hoehne A,James ML,Alam IS,Ronald JA,Schneider B,D'Souza A,Witney TH,Andrews LE,Cropper HC,Behera D,Gowrishankar G,Ding Z,Wyss-Coray T,Chin FT,Biswal S,Gambhir SS

    更新日期：2018-02-22 00:00:00

  • Hippocampal microglial activation triggers a neurotoxic-specific astrocyte response and mediates etomidate-induced long-term synaptic inhibition.

    abstract：BACKGROUND:Accumulating evidence has highlighted the importance of microglial and astrocyte responses in the pathological development of postoperative cognitive dysfunction (POCD). However, the mechanisms involved are not well understood. METHODS:A perioperative neurocognitive disorders (PND) mouse model was generated...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-020-01799-0

    authors： Li D,Chen M,Meng T,Fei J

    更新日期：2020-04-07 00:00:00

  • Protein kinase C-delta inhibition protects blood-brain barrier from sepsis-induced vascular damage.

    abstract：BACKGROUND:Neuroinflammation often develops in sepsis leading to activation of cerebral endothelium, increased permeability of the blood-brain barrier (BBB), and neutrophil infiltration. We have identified protein kinase C-delta (PKCδ) as a critical regulator of the inflammatory response and demonstrated that pharmacol...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-018-1342-y

    authors： Tang Y,Soroush F,Sun S,Liverani E,Langston JC,Yang Q,Kilpatrick LE,Kiani MF

    更新日期：2018-11-06 00:00:00

  • Scolopendra subspinipes mutilans attenuates neuroinflammation in symptomatic hSOD1(G93A) mice.

    abstract：BACKGROUND:Amyotrophic lateral sclerosis (ALS) is a progressive, adult-onset neurodegenerative disorder characterized by selective motor neuron death in the spinal cord, brainstem, and motor cortex. Neuroinflammation is one of several pathological causes of degenerating motor neurons and is induced by activated microgl...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/1742-2094-10-131

    authors： Cai M,Choi SM,Song BK,Son I,Kim S,Yang EJ

    更新日期：2013-10-29 00:00:00

  • Evidence for the involvement of gamma delta T cells in the immune response in Rasmussen encephalitis.

    abstract：BACKGROUND:Rasmussen encephalitis (RE) is a rare neuroinflammatory disease characterized by intractable seizures and progressive atrophy on one side of the cerebrum. Perivascular cuffing and clusters of T cells in the affected cortical hemisphere are indicative of an active cellular immune response. METHODS:Peripheral...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-015-0352-2

    authors： Owens GC,Erickson KL,Malone CC,Pan C,Huynh MN,Chang JW,Chirwa T,Vinters HV,Mathern GW,Kruse CA

    更新日期：2015-07-19 00:00:00

  • Combining nitric oxide release with anti-inflammatory activity preserves nigrostriatal dopaminergic innervation and prevents motor impairment in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson's disease.

    abstract：BACKGROUND:Current evidence suggests a role of neuroinflammation in the pathogenesis of Parkinson's disease (PD) and in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model of basal ganglia injury. Reportedly, nonsteroidal anti-inflammatory drugs (NSAIDs) mitigate DAergic neurotoxicity in rodent models of PD. ...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/1742-2094-7-83

    authors： L'Episcopo F,Tirolo C,Caniglia S,Testa N,Serra PA,Impagnatiello F,Morale MC,Marchetti B

    更新日期：2010-11-23 00:00:00

  • Role of the indoleamine-2,3-dioxygenase/kynurenine pathway of tryptophan metabolism in behavioral alterations in a hepatic encephalopathy rat model.

    abstract：BACKGROUND:This study aims to explore the role of indoleamine-2,3-dioxygenase (IDO)/kynurenine (KYN) pathway of tryptophan (TRY) metabolism in behavioral alterations observed in hepatic encephalopathy (HE) rats. METHODS:Expression levels of proinflammatory cytokines were tested by QT-PCR and ELISA, levels of IDOs were...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-017-1037-9

    authors： Jiang X,Xu L,Tang L,Liu F,Chen Z,Zhang J,Chen L,Pang C,Yu X

    更新日期：2018-01-04 00:00:00

  • Three-dimensional morphometric analysis reveals time-dependent structural changes in microglia and astrocytes in the central amygdala and hypothalamic paraventricular nucleus of heart failure rats.

    abstract：BACKGROUND:Cardiovascular diseases, including heart failure, are the most common cause of death globally. Recent studies support a high degree of comorbidity between heart failure and cognitive and mood disorders resulting in memory loss, depression, and anxiety. While neuroinflammation in the hypothalamic paraventricu...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-020-01892-4

    authors： Althammer F,Ferreira-Neto HC,Rubaharan M,Roy RK,Patel AA,Murphy A,Cox DN,Stern JE

    更新日期：2020-07-23 00:00:00

  • Differential expression of Cathepsin E in transthyretin amyloidosis: from neuropathology to the immune system.

    abstract：BACKGROUND:Increasing evidence supports a key role for inflammation in the neurodegenerative process of familial amyloidotic polyneuropathy (FAP). While there seems to be an overactivation of the neuronal interleukin-1 signaling pathway, the immune response is apparently compromised in FAP. Accordingly, little immune c...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-017-0891-9

    authors： Gonçalves NP,Moreira J,Martins D,Vieira P,Obici L,Merlini G,Saraiva M,Saraiva MJ

    更新日期：2017-06-06 00:00:00

  • Increased levels of HMGB1 and pro-inflammatory cytokines in children with febrile seizures.

    abstract：OBJECTIVE:Febrile seizures are the most common form of childhood seizures. Fever is induced by pro-inflammatory cytokines during infection, and pro-inflammatory cytokines may trigger the development of febrile seizures. In order to determine whether active inflammation, including high mobility group box-1 (HMGB1) and p...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/1742-2094-8-135

    authors： Choi J,Min HJ,Shin JS

    更新日期：2011-10-11 00:00:00

  • Mannan binding lectin-associated serine protease-2 (MASP-2) critically contributes to post-ischemic brain injury independent of MASP-1.

    abstract：BACKGROUND:Complement activation via the lectin activation pathway (LP) has been identified as the key mechanism behind post-ischemic tissue inflammation causing ischemia-reperfusion injury (IRI) which can significantly impact the clinical outcome of ischemic disease. This work defines the contributions of each of the ...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-016-0684-6

    authors： Orsini F,Chrysanthou E,Dudler T,Cummings WJ,Takahashi M,Fujita T,Demopulos G,De Simoni MG,Schwaeble W

    更新日期：2016-08-30 00:00:00

  • Virus-mediated EpoR76E gene therapy preserves vision in a glaucoma model by modulating neuroinflammation and decreasing oxidative stress.

    abstract：BACKGROUND:Glaucoma is a complex neurodegeneration and a leading cause of blindness worldwide. Current therapeutic strategies, which are all directed towards lowering the intraocular pressure (IOP), do not stop progression of the disease. We have demonstrated that recombinant adeno-associated virus (rAAV) gene delivery...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-016-0499-5

    authors： Hines-Beard J,Bond WS,Backstrom JR,Rex TS

    更新日期：2016-02-15 00:00:00

  • Monomethyl fumarate promotes Nrf2-dependent neuroprotection in retinal ischemia-reperfusion.

    abstract：BACKGROUND:Retinal ischemia results in neuronal degeneration and contributes to the pathogenesis of multiple blinding diseases. Recently, the fumaric acid ester dimethyl fumarate (DMF) has been FDA-approved for the treatment of multiple sclerosis, based on its neuroprotective and anti-inflammatory effects. Its potentia...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-015-0452-z

    authors： Cho H,Hartsock MJ,Xu Z,He M,Duh EJ

    更新日期：2015-12-21 00:00:00

  • Anti-inflammatory and anti-oxidant mechanisms of an MMP-8 inhibitor in lipoteichoic acid-stimulated rat primary astrocytes: involvement of NF-κB, Nrf2, and PPAR-γ signaling pathways.

    abstract：BACKGROUND:Recent evidence suggests that reactive astrocytes play an important role in neuroinflammation and neurodegenerative diseases. Thus, controlling astrocyte reactivity has been suggested as a promising strategy for treating neurodegenerative diseases. In the present study, we investigated whether a matrix metal...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-018-1363-6

    authors： Lee EJ,Park JS,Lee YY,Kim DY,Kang JL,Kim HS

    更新日期：2018-11-23 00:00:00

  • Atorvastatin prevents age-related and amyloid-β-induced microglial activation by blocking interferon-γ release from natural killer cells in the brain.

    abstract：BACKGROUND:Microglial function is modulated by several factors reflecting the numerous receptors expressed on the cell surface, however endogenous factors which contribute to the age-related increase in microglial activation remain largely unknown. One possible factor which may contribute is interferon-γ (IFNγ). IFNγ h...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/1742-2094-8-27

    authors： Lyons A,Murphy KJ,Clarke R,Lynch MA

    更新日期：2011-03-31 00:00:00

  • Loss of mural cell-derived laminin aggravates hemorrhagic brain injury.

    abstract：BACKGROUND:Mural cells synthesize and deposit laminin to the basement membrane. To investigate the function of mural cell-derived laminin, we generated a mutant mouse line lacking mural cell-derived laminin (termed PKO). In a previous study, we showed that the PKO mice were grossly normal under homeostatic condition, b...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-020-01788-3

    authors： Gautam J,Xu L,Nirwane A,Nguyen B,Yao Y

    更新日期：2020-04-06 00:00:00

  • Blast traumatic brain injury and serum inflammatory cytokines: a repeated measures case-control study among U.S. military service members.

    abstract：BACKGROUND:There is a paucity of human data on exposure to blast traumatic brain injury (bTBI) and the corresponding systemic cytokine immune response at later time points (i.e., months, years) post-injury. METHODS:We conducted a repeated measures, case-control study, examining associations of serum levels of pro- and...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-019-1624-z

    authors： Rusiecki J,Levin LI,Wang L,Byrne C,Krishnamurthy J,Chen L,Galdzicki Z,French LM

    更新日期：2020-01-13 00:00:00

  • Ecto-5'-nucleotidase (CD73) attenuates inflammation after spinal cord injury by promoting macrophages/microglia M2 polarization in mice.

    abstract：BACKGROUND:Immune activation, specifically activation of macrophages and resident microglia, leading to inflammation is a key component in the progression of spinal cord injury (SCI). Macrophages/microglia exist in two states-the classically activated M1 phenotype that confers pro-inflammatory effects or the alternativ...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-018-1183-8

    authors： Xu S,Zhu W,Shao M,Zhang F,Guo J,Xu H,Jiang J,Ma X,Xia X,Zhi X,Zhou P,Lu F

    更新日期：2018-05-22 00:00:00

  • Activation of the NLRP3 inflammasome in lipopolysaccharide-induced mouse fatigue and its relevance to chronic fatigue syndrome.

    abstract：BACKGROUND:The NLRP3 inflammasome (NOD-like receptor family, pyrin domain containing 3) is an intracellular protein complex that plays an important role in innate immune sensing. Its activation leads to the maturation of caspase-1 and regulates the cleavage of interleukin (IL)-1β and IL-18. Various studies have shown t...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-016-0539-1

    authors： Zhang ZT,Du XM,Ma XJ,Zong Y,Chen JK,Yu CL,Liu YG,Chen YC,Zhao LJ,Lu GC

    更新日期：2016-04-05 00:00:00

  • Neuroinflammation in autism spectrum disorders.

    abstract：OBJECTIVES:The neurobiological basis for autism remains poorly understood. However, research suggests that environmentalfactors and neuroinflammation, as well as genetic factors, are contributors. This study aims to test the role that might be played by heat shock protein (HSP)70, transforming growth factor (TGF)-β2, C...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/1742-2094-9-265

    authors： El-Ansary A,Al-Ayadhi L

    更新日期：2012-12-11 00:00:00

  • Activin A increases phagocytosis of Escherichia coli K1 by primary murine microglial cells activated by toll-like receptor agonists.

    abstract：BACKGROUND:Bacterial meningitis is associated with high mortality and long-term neurological sequelae. Increasing the phagocytic activity of microglia could improve the resistance of the CNS against infections. We studied the influence of activin A, a member of the TGF-β family with known immunoregulatory and neuroprot...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-018-1209-2

    authors： Diesselberg C,Ribes S,Seele J,Kaufmann A,Redlich S,Bunkowski S,Hanisch UK,Michel U,Nau R,Schütze S

    更新日期：2018-06-07 00:00:00

  • DHCR24 exerts neuroprotection upon inflammation-induced neuronal death.

    abstract：BACKGROUND:DHCR24, involved in the de novo synthesis of cholesterol and protection of neuronal cells against different stress conditions, has been shown to be selectively downregulated in neurons of the affected brain areas in Alzheimer's disease. METHODS:Here, we investigated whether the overexpression of DHCR24 prot...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-017-0991-6

    authors： Martiskainen H,Paldanius KMA,Natunen T,Takalo M,Marttinen M,Leskelä S,Huber N,Mäkinen P,Bertling E,Dhungana H,Huuskonen M,Honkakoski P,Hotulainen P,Rilla K,Koistinaho J,Soininen H,Malm T,Haapasalo A,Hiltunen M

    更新日期：2017-11-07 00:00:00

  • LPS-induced systemic inflammation reveals an immunomodulatory role for the prion protein at the blood-brain interface.

    abstract：BACKGROUND:The cellular prion protein (PrPC) is an evolutionary conserved protein abundantly expressed not only in the central nervous system but also peripherally including the immune system. A line of Norwegian dairy goats naturally devoid of PrPC (PRNP Ter/Ter) provides a novel model for studying PrPC physiology. M...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-017-0879-5

    authors： Salvesen Ø,Reiten MR,Espenes A,Bakkebø MK,Tranulis MA,Ersdal C

    更新日期：2017-05-22 00:00:00

  • Neuroprotective effects of bilobalide on cerebral ischemia and reperfusion injury are associated with inhibition of pro-inflammatory mediator production and down-regulation of JNK1/2 and p38 MAPK activation.

    abstract：BACKGROUND:Mitogen-activated protein kinase (MAPK) signaling pathways are implicated in inflammatory and apoptotic processes of cerebral ischemia and reperfusion (I/R) injury. Hence, MAPK pathways represent a promising therapeutic target. Exploring the full potential of inhibitors of MAPK pathways is a useful therapeut...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-014-0167-6

    authors： Jiang M,Li J,Peng Q,Liu Y,Liu W,Luo C,Peng J,Li J,Yung KK,Mo Z

    更新日期：2014-09-26 00:00:00

  • Lentivirus-mediated interleukin-1β (IL-1β) knock-down in the hippocampus alleviates lipopolysaccharide (LPS)-induced memory deficits and anxiety- and depression-like behaviors in mice.

    abstract：BACKGROUND:Recent evidence has suggested that peripheral inflammatory responses induced by lipopolysaccharides (LPS) play an important role in neuropsychiatric dysfunction in rodents. Interleukin-1β (IL-1β), a pro-inflammatory cytokine, has been proposed to be a key mediator in a variety of behavioral dysfunction induc...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-017-0964-9

    authors： Li M,Li C,Yu H,Cai X,Shen X,Sun X,Wang J,Zhang Y,Wang C

    更新日期：2017-09-20 00:00:00

  • Mossy cell hypertrophy and synaptic changes in the hilus following mild diffuse traumatic brain injury in pigs.

    abstract：BACKGROUND:Each year in the USA, over 2.4 million people experience mild traumatic brain injury (TBI), which can induce long-term neurological deficits. The dentate gyrus of the hippocampus is notably susceptible to damage following TBI, as hilar mossy cell changes in particular may contribute to post-TBI dysfunction. ...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-020-1720-0

    authors： Grovola MR,Paleologos N,Wofford KL,Harris JP,Browne KD,Johnson V,Duda JE,Wolf JA,Cullen DK

    更新日期：2020-01-31 00:00:00

  • Discovery of novel L-type voltage-gated calcium channel blockers and application for the prevention of inflammation and angiogenesis.

    abstract：BACKGROUND:The ways in which microglia activate and promote neovascularization (NV) are not fully understood. Recent in vivo evidence supports the theory that calcium is required for the transition of microglia from a surveillance state to an active one. The objectives of this study were to discover novel L-type voltag...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-020-01801-9

    authors： Saddala MS,Lennikov A,Mukwaya A,Yang Y,Hill MA,Lagali N,Huang H

    更新日期：2020-04-25 00:00:00

  • High serum levels of transforming growth factor β1 are associated with increased cortical thickness in cingulate and right frontal areas in healthy subjects.

    abstract：BACKGROUND:Transforming growth factor β (TGF-β) is a cytokine having multiple functions in the central nervous system such as promoting repair mechanisms in degenerative diseases and stroke. To date, however, its neuroprotective effects in non-disease conditions have not been studied METHODS:With the aim of exploring ...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/1742-2094-9-42

    authors： Piras F,Salani F,Bossù P,Caltagirone C,Spalletta G

    更新日期：2012-02-28 00:00:00

  • Sulforaphane rescues amyloid-β peptide-mediated decrease in MerTK expression through its anti-inflammatory effect in human THP-1 macrophages.

    abstract：BACKGROUND:Mer tyrosine kinase (MerTK) activity necessary for amyloid-stimulated phagocytosis strongly implicates that MerTK dysregulation might contribute to chronic inflammation implicated in Alzheimer's disease (AD) pathology. However, the precise mechanism involved in the regulation of MerTK expression by amyloid-β...

    journal_title：Journal of neuroinflammation

    pub_type： 杂志文章

    doi：10.1186/s12974-018-1112-x

    authors： Jhang KA,Park JS,Kim HS,Chong YH

    更新日期：2018-03-12 00:00:00