Abstract：
:In the present study, we constructed the recombinant plasmid IL10-PEGFP-C1 and successfully transfected into human mesenchymal stem cells. After culturing for 72 h, the levels of IL6 and TNF-α in the supernatant of the MSCs-IL10 group were significantly lower than the vector group and the control group (17.6 ± 0.68vs73.8 ± 0.8 and 74.4 ± 1.5) µg/L and (65.05 ± 3.8 vs 203.2 ± 2.4 and 201.3 ± 3.7) µg/L, respectively (p < 0.001) .The animal experiments showed that the volume of subcutaneous tumors in the MSCs-IL10 group in vivo was a significantly less level compared to that in MSC control and the blank control groups (76.84 ± 20.11) mm3 vs (518. 344 ± 48.66) mm3, (576.99± 49.88) mm3, (P < 0. 05) and they have a longer life time. Further we found the mass concentrations of IL6 and TNF-α in the blood serum of MSC-IL10 group were lower than the vector group and the control group (64.42 ± 10.9 vs120.83 ± 15.52 and 122.65 ± 13.71) and (40.05 ± 5.63 vs 126.78 ±1.89 and 105.83 ± 2.16) µg/L respectively (p < 0.001). CD31 immunohistochemistry and alginate encapsulation experiments showed tumor angiogenesis were inhibited in MSCs-IL10 group in comparison to the control and vector group (P < 0.001), FITC-labeled dextran intake was also lower than the other groups (P < 0.01). Collectively, this study suggested IL10 could inhibit the growth of the transplanted tumor in vivo and prolong survival of mice, and the primary mechanism may be the indirect inhibition of pro-inflammatory cytokines IL6 and TNF-α secretion and tumor angiogenesis formation.
journal_name
J Cancerjournal_title
Journal of Cancerauthors
Zhao C,Pu Y,Zhang H,Hu X,Zhang R,He S,Zhao Q,Mu Bdoi
10.7150/jca.38062subject
Has Abstractpub_date
2020-07-09 00:00:00pages
5345-5352issue
18issn
1837-9664pii
jcav11p5345journal_volume
11pub_type
杂志文章abstract：:Thyroid transcription factor-1 (TTF-1) is overexpressed in up to 95% of primary lung adenocarcinoma while negative for almost all squamous cell carcinomas. TTF-1 expression has been investigated as a prognostic factor in non-small-cell lung cancer (NSCLC) with conflicting results. We conducted this meta-analysis to ga...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.26830
更新日期：2018-10-20 00:00:00
abstract：:Ovarian cancer, cervical cancer and endometrial cancer are three relatively common malignant cancers of the female reproductive system. Despite improvements in female genital tract cancer detection and development of new therapeutic approaches, there are still poor prognoses and some do not respond to therapeutic patt...
journal_title：Journal of Cancer
pub_type： 杂志文章,评审
doi：10.7150/jca.44727
更新日期：2020-10-21 00:00:00
abstract：:Objectives: Chordomas are slow-growing malignancies that commonly affect vital neurological structures. These neoplasms are highly resistant to current chemotherapeutic regimens and often recur after surgical intervention. Therefore, there is an urgent need to identify molecular targets and more robust drugs to improv...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.35426
更新日期：2020-01-01 00:00:00
abstract：:Background: Population-based data on the prognosis of brain metastases at initial diagnosis of ovary cancer (OCBM) are currently lacking. Besides, the effective treatment for OCBM patients is still controversial now. The study aimed to explore the prognostic factors among OCBM. Methods: We retrospectively reviewed the...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.44494
更新日期：2020-05-18 00:00:00
abstract：:Background: The programmed death 1 (PD1)/programmed death ligand 1 (PDL1) targeted therapies have gained positive outcomes in several tumors, but the evidence of the expression and prognosis value of PD1/PDL1 in high risk prostate cancer was rare. Methods: Immunohistochemical analysis of PDL1/PD1 expression by a valid...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.30384
更新日期：2019-06-02 00:00:00
abstract：:Esophageal cancer (EC) is a unique and heterogeneous disease diagnosed mostly at advanced stages. Altered glycans presented on cell surfaces are involved in the occurrence and development of malignancy. However, the effects of glycans on EC progression are largely unexplored. Here, a lectin array was utilized to detec...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.43806
更新日期：2020-06-01 00:00:00
abstract：:Background: Identification of new predictive markers in melanoma is of great clinical importance. This study was aimed to analyze association between selected common variants in the cancer susceptibility genes and melanoma progression at the time of diagnosis. Material and Method: The study included 243 consecutive pa...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.26404
更新日期：2018-08-06 00:00:00
abstract：:Cancer cells express epithelial markers, and when progressing in malignancy they may express markers of the mesenchymal cell type. Therefore an epithelial-mesenchymal transition of the cancer cells is assumed. However the mesenchymal markers can equally well be interpreted as myeloid markers since they are common in b...
journal_title：Journal of Cancer
pub_type： 杂志文章,评审
doi：10.7150/jca.8242
更新日期：2014-01-15 00:00:00
abstract：:Objectives: Platinum-based chemotherapy is first-line treatment for non-small cell lung cancer (NSCLC) patients. The efficacy is limited by drug resistance. Recent studies suggest that ATP7B, a copper efflux transporter, may be involved in platinum resistance. However, the clinical significance of ATP7B expression in ...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.26286
更新日期：2018-09-08 00:00:00
abstract：:Even with the technological advances of dose-escalated IMRT with the addition of the latest image guidance technologies, local failures still occur. The combination of MRI-based imaging techniques can yield quantitative information that reflects on the biological properties of prostatic tissues. These techniques provi...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.5419
更新日期：2013-01-01 00:00:00
abstract：:Many preclinical and clinical studies are currently evaluating metformin in combination with classical therapeutic agents as anti-cancer therapy. In this study we used three distinct pancreatic cancer cell lines and evaluated cell death by trypan blue assay and Western Blots using antibodies directed against cleaved c...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.17972
更新日期：2017-07-01 00:00:00
abstract：:Studies have indicated the significance of tumor associated macrophages (TAMs) in breast cancer; however, inconsistent results still exist. We retrospectively reviewed the macrophage distribution in 1579 breast cancer specimens with anti-CD68 or anti-CD163 immunohistochemical staining, and further analyzed the overall...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.33914
更新日期：2019-07-23 00:00:00
abstract：PURPOSE:To evaluate acute hypersensitivity reactions at the UCSD Moores Cancer Center in San Diego, compare our findings to those reported previously in the literature, and examine the effectiveness of the objective grading scale as represented by the Common Terminology Criteria for Adverse Events (CTCAE). PATIENTS AN...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.2.153
更新日期：2011-03-10 00:00:00
abstract：:Objective: This study aimed to evaluate the therapeutic response of hepatocellular carcinoma (HCC) after transcatheter arterial chemoembolization (TACE) with diffusion kurtosis imaging (DKI). Methods: Forty-three patients with fifty-nine hepatic cancer nodules were recruited for this study. All patients were treated b...
journal_title：Journal of Cancer
pub_type： 杂志文章,评审
doi：10.7150/jca.32491
更新日期：2020-02-10 00:00:00
abstract：:Colorectal cancer (CRC) is the fifth leading cause of cancer-related death in China. The incidence of Chinese CRC has increased dramatically with the changes of dietary and lifestyle. However, the genetic landscape of Chinese colorectal cancer mutation is still poorly understood. In this study, we have performed whole...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.37017
更新日期：2020-01-01 00:00:00
abstract：:Clinical outcomes of checkpoint blockade immunotherapy on colorectal cancer (CRC) are influenced by mismatch repair (MMR) gene status, which is associated with distinct tumor immune infiltrates and systemic inflammatory response status. However, the prognostic value of PD-L1 expression and the systemic inflammatory re...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.25187
更新日期：2019-04-05 00:00:00
abstract：:Philadelphia chromosome-positive (Ph+) leukemia is characterized by reciprocal translocation between chromosomes 9 and 22. The resultant BCR/ABL fusion protein displays constitutive tyrosine kinase activity, leading to the induction of aberrant proliferation and neoplastic transformation. The bone marrow (BM) microenv...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.19633
更新日期：2017-10-23 00:00:00
abstract：:Background and aims. Cyclin-dependent kinase inhibitor 3 (CDKN3) has been found playing a varying role in carcinogenesis, but its biological function in esophageal squamous cell carcinoma (ESCC) is unclear. The aim of this study was to demonstrate the role of CDKN3 in ESCC. Materials and Methods: Real-time PCR and Wes...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.27053
更新日期：2019-04-21 00:00:00
abstract：:Oncogenic activation of the ETS Related Gene (ERG) in humans was originally identified in subsets of Ewing sarcomas, myeloid leukemias and, recently, in the majority of prostate cancers. Expression of human ERG protein and consequently its functions in normal and disease states needs to be better understood in light o...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.1.197
更新日期：2010-10-25 00:00:00
abstract：:Multidrug resistance is a major obstacle to the effective treatment of esophageal carcinoma. It occurs more readily in hypoxia and acidosis microenvironment. TTLL6 is one of Tubulin tyrosine ligase-like family members. In this study, the effect of TTLL6 on the regulation of cisplatin (CDDP) sensitivity was evaluated i...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.47694
更新日期：2020-09-30 00:00:00
abstract：:Cellular compartmentalization of biochemical processes in eukaryotic cells is critical for many functions including shuttling of reducing equivalents across membranes. Although coordination of metabolic flux between different organelles is vital for cell physiology, its impact on tumor cell survival is not well unders...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.19373
更新日期：2017-07-05 00:00:00
abstract：:Endometrium is the mucosal lining of the uterus which expressed a cyclic process of proliferation, secretion and scaling under the control of hormones secreted by the ovary, and it also plays an indispensable role in the embryo implantation, the constitution of fetal-maternal interface, and the maintaining of pregnanc...
journal_title：Journal of Cancer
pub_type： 杂志文章,评审
doi：10.7150/jca.31742
更新日期：2019-06-09 00:00:00
abstract：:Background: Most hepatocellular carcinoma (HCC) patients have undergone a progression from chronic hepatitis, then liver cirrhosis (LC), and finally to carcinoma. The objective of this study was to elucidate risk factors to predict HCC development for cirrhosis patients. Methods: Multiple methylated specific PCR (MSP)...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.24024
更新日期：2018-06-04 00:00:00
abstract：PURPOSE:We conducted a meta-analysis to evaluate and compare the short- and long-term results of laparoscopic colorectal surgery (LCRS) and conventional open surgery (OCRS) for colorectal cancer (CRC). METHODS:We searched relevant papers published between January 1990 and May 2011. We analyzed the outcomes of each typ...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.2.425
更新日期：2011-01-01 00:00:00
abstract：:Background: The incidence and mortality rate of gastric cancer has markedly declined over the past few decades, due to the progress and advances in the development of diagnostic and treatment regimens. However, there is still a large portion of patients who are first diagnosed during the advanced stage of gastric canc...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.31303
更新日期：2019-10-06 00:00:00
abstract：UNLABELLED:The mechanism of progression from ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC) remains largely unknown. We compared gene expression in tumors with simultaneous DCIS and IDC to decipher how diverse proteins participate in the local invasive process.Twenty frozen tumor specimens with conc...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.4120
更新日期：2012-01-01 00:00:00
abstract：:Objective: To enquire into the clinical significance and potential molecular mechanism of microRNA (miRNA)-196b-5p in hepatocellular carcinoma (HCC). Methods: Quantitative reverse transcription and polymerase chain reaction (qRT-PCR) were utilized to examine miR-196b-5p expression level in 67 HCC paraffin embedded tis...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.29293
更新日期：2019-08-29 00:00:00
abstract：:Minichromosome Maintenance (MCM) proteins play essential roles in various cancers. We previously reported that MCM7 could be a prognostic biomarker in non-small cell lung cancer (NSCLC). The purpose of current study is to explore roles of other MCM proteins in NSCLC and their correlation with clinico-pathologic parame...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.20777
更新日期：2017-10-09 00:00:00
abstract：:Background: To support cancer therapy, development of low cost library preparation techniques for targeted next generation sequencing (NGS) is needed. In this study we designed and tested a PCR-based library preparation panel with limited target area for sequencing the top 12 somatic mutation hot spots in colorectal c...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.16037
更新日期：2017-01-12 00:00:00
abstract：:Glioblastoma multiforme (GBM) is commonly known as the most aggressive primary CNS tumor in adults. The mean survival of it is 14 to 15 months, following the standard therapy from surgery, chemotherapy, to radiotherapy. Efforts in recent decades have brought many novel therapies to light, however, with limitations. In...
journal_title：Journal of Cancer
pub_type： 杂志文章,评审
doi：10.7150/jca.32475
更新日期：2019-08-27 00:00:00
