Protein Kinase C Inhibition Mediates Neuroblast Enrichment in Mechanical Brain Injuries.

Abstract:

:Brain injuries of different etiologies lead to irreversible neuronal loss and persisting neuronal deficits. New therapeutic strategies are emerging to compensate neuronal damage upon brain injury. Some of these strategies focus on enhancing endogenous generation of neurons from neural stem cells (NSCs) to substitute the dying neurons. However, the capacity of the injured brain to produce new neurons is limited, especially in cases of extensive injury. This reduced neurogenesis is a consequence of the effect of signaling molecules released in response to inflammation, which act on intracellular pathways, favoring gliogenesis and preventing recruitment of neuroblasts from neurogenic regions. Protein kinase C (PKC) is a family of intracellular kinases involved in several of these gliogenic signaling pathways. The aim of this study was to analyze the role of PKC isozymes in the generation of neurons from neural progenitor cells (NPCs) in vitro and in vivo in brain injuries. PKC inhibition in vitro, in cultures of NPC isolated from the subventricular zone (SVZ) of postnatal mice, leads differentiation towards a neuronal fate. This effect is not mediated by classical or atypical PKC. On the contrary, this effect is mediated by novel PKCε, which is abundantly expressed in NPC cultures under differentiation conditions. PKCε inhibition by siRNA promotes neuronal differentiation and reduces glial cell differentiation. On the contrary, inhibition of PKCθ exerts a small anti-gliogenic effect and reverts the effect of PKCε inhibition on neuronal differentiation when both siRNAs are used in combination. Interestingly, in cortical brain injuries we have found expression of almost all PKC isozymes found in vitro. Inhibition of PKC activity in this type of injuries leads to neuronal production. In conclusion, these findings show an effect of PKCε in the generation of neurons from NPC in vitro, and they highlight the role of PKC isozymes as targets to produce neurons in brain lesions.

journal_name

Front Cell Neurosci

authors

García-Bernal F,Geribaldi-Doldán N,Domínguez-García S,Carrasco M,Murillo-Carretero M,Delgado-Ariza A,Díez-Salguero M,Verástegui C,Castro C

doi

10.3389/fncel.2018.00462

subject

Has Abstract

pub_date

2018-11-27 00:00:00

pages

462

issn

1662-5102

journal_volume

12

pub_type

杂志文章
  • Overall Assay of Neuronal Signal Propagation Pattern With Long-Term Potentiation (LTP) in Hippocampal Slices From the CA1 Area With Fast Voltage-Sensitive Dye Imaging.

    abstract::Activity-dependent changes in the input-output (I-O) relationship of a neural circuit are central in the learning and memory function of the brain. To understand circuit-wide adjustments, optical imaging techniques to probe the membrane potential at every component of neurons, such as dendrites, axons and somas, in th...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2018.00389

    authors: Tominaga Y,Taketoshi M,Tominaga T

    更新日期:2018-10-24 00:00:00

  • Cytoplasmic TDP43 Binds microRNAs: New Disease Targets in Amyotrophic Lateral Sclerosis.

    abstract::Amyotrophic lateral sclerosis (ALS) is a progressive, fatal, and incurable neurodegenerative disease. Recent studies suggest that dysregulation of gene expression by microRNAs (miRNAs) may play an important role in ALS pathogenesis. The reversible nature of this dysregulation makes miRNAs attractive pharmacological ta...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2020.00117

    authors: Paez-Colasante X,Figueroa-Romero C,Rumora AE,Hur J,Mendelson FE,Hayes JM,Backus C,Taubman GF,Heinicke L,Walter NG,Barmada SJ,Sakowski SA,Feldman EL

    更新日期:2020-05-12 00:00:00

  • Plasticity in the Drosophila larval visual system.

    abstract::The remarkable ability of the nervous system to modify its structure and function is mostly experience and activity modulated. The molecular basis of neuronal plasticity has been studied in higher behavioral processes, such as learning and memory formation. However, neuronal plasticity is not restricted to higher brai...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2013.00105

    authors: Farca-Luna AJ,Sprecher SG

    更新日期:2013-07-04 00:00:00

  • Enhanced Axonal Extension of Subcortical Projection Neurons Isolated from Murine Embryonic Cortex using Neuropilin-1.

    abstract::The cerebral cortical tissue of murine embryo and pluripotent stem cell (PSC)-derived neurons can survive in the brain and extend axons to the spinal cord. For efficient cell integration to the corticospinal tract (CST) after transplantation, the induction or selection of cortical motor neurons is important. However, ...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2017.00123

    authors: Sano N,Shimogawa T,Sakaguchi H,Ioroi Y,Miyawaki Y,Morizane A,Miyamoto S,Takahashi J

    更新日期:2017-05-01 00:00:00

  • Super Resolution Imaging of Genetically Labeled Synapses in Drosophila Brain Tissue.

    abstract::Understanding synaptic connectivity and plasticity within brain circuits and their relationship to learning and behavior is a fundamental quest in neuroscience. Visualizing the fine details of synapses using optical microscopy remains however a major technical challenge. Super resolution microscopy opens the possibili...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2016.00142

    authors: Spühler IA,Conley GM,Scheffold F,Sprecher SG

    更新日期:2016-05-26 00:00:00

  • G Protein-Coupled Receptor Heteromers as Putative Pharmacotherapeutic Targets in Autism.

    abstract::A major challenge in the development of pharmacotherapies for autism is the failure to identify pathophysiological mechanisms that could be targetable. The majority of developing strategies mainly aim at restoring the brain excitatory/inhibitory imbalance described in autism, by targeting glutamate or GABA receptors. ...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章,评审

    doi:10.3389/fncel.2020.588662

    authors: DelaCuesta-Barrutia J,Peñagarikano O,Erdozain AM

    更新日期:2020-10-30 00:00:00

  • Rifampicin Attenuated Global Cerebral Ischemia Injury via Activating the Nuclear Factor Erythroid 2-Related Factor Pathway.

    abstract::Background: Recent studies have found that rifampicin has neuroprotective properties in neurodegenerative diseases. However, the exact mechanisms of action remain unclear. The nuclear factor erythroid 2-related factor 2 (Nrf2) has been considered a potential target for neuroprotection. In this study, we examined wheth...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2016.00273

    authors: Chen B,Cao H,Chen L,Yang X,Tian X,Li R,Cheng O

    更新日期:2016-11-29 00:00:00

  • Distinct Residential and Infiltrated Macrophage Populations and Their Phagocytic Function in Mild and Severe Neonatal Hypoxic-Ischemic Brain Damage.

    abstract::Neonatal brain injury, especially severe injury induced by hypoxia-ischemia (HI), causes mortality and long-term neurological impairments. Our previous study demonstrated activation of CD11b+ myeloid cells, including residential microglial cells (MGs) and infiltrating monocyte-derived macrophages (MDMs) in a murine mo...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2020.00244

    authors: Min Y,Yan L,Wang Q,Wang F,Hua H,Yuan Y,Jin H,Zhang M,Zhao Y,Yang J,Jiang X,Yang Y,Li F

    更新日期:2020-08-10 00:00:00

  • Implications of glial nitric oxide in neurodegenerative diseases.

    abstract::Nitric oxide (NO) is a pleiotropic janus-faced molecule synthesized by nitric oxide synthases (NOS) which plays a critical role in a number of physiological and pathological processes in humans. The physiological roles of NO depend on its local concentrations, as well as its availability and the nature of downstream t...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章,评审

    doi:10.3389/fncel.2015.00322

    authors: Yuste JE,Tarragon E,Campuzano CM,Ros-Bernal F

    更新日期:2015-08-17 00:00:00

  • MicroRNA-210 Protects PC-12 Cells Against Hypoxia-Induced Injury by Targeting BNIP3.

    abstract::MicroRNA (miR)-210 is the most consistently and predominantly up-regulated miR in response to hypoxia in multiple cancer cells. The roles of miR-210 in rat adrenal gland pheochromocytoma (PC-12) cells remain unknown. We aimed to explore the possible effect of miR-210 in neonatal brain injury. We explored the potential...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2017.00285

    authors: Luan Y,Zhang X,Zhang Y,Dong Y

    更新日期:2017-09-22 00:00:00

  • A Model of Germinal Matrix Hemorrhage in Preterm Rat Pups.

    abstract::Germinal matrix hemorrhage (GMH) is a serious complication in extremely preterm infants associated with neurological deficits and mortality. The purpose of the present study was to develop and characterize a grade III and IV GMH model in postnatal day 5 (P5) rats, the equivalent of preterm human brain maturation. P5 W...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2020.535320

    authors: Jinnai M,Koning G,Singh-Mallah G,Jonsdotter A,Leverin AL,Svedin P,Nair S,Takeda S,Wang X,Mallard C,Ek CJ,Rocha-Ferreira E,Hagberg H

    更新日期:2020-12-03 00:00:00

  • Firing Properties of Genetically Identified Dorsal Raphe Serotonergic Neurons in Brain Slices.

    abstract::Tonic spiking of serotonergic neurons establishes serotonin levels in the brain. Since the first observations, slow regular spiking has been considered as a defining feature of serotonergic neurons. Recent studies, however, have revealed the heterogeneity of serotonergic neurons at multiple levels, comprising their el...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2016.00195

    authors: Mlinar B,Montalbano A,Piszczek L,Gross C,Corradetti R

    更新日期:2016-08-03 00:00:00

  • Quantifying mechanical force in axonal growth and guidance.

    abstract::Mechanical force plays a fundamental role in neuronal development, physiology, and regeneration. In particular, research has shown that force is involved in growth cone-mediated axonal growth and guidance as well as stretch-induced elongation when an organism increases in size after forming initial synaptic connection...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章,评审

    doi:10.3389/fncel.2015.00359

    authors: Athamneh AI,Suter DM

    更新日期:2015-09-16 00:00:00

  • The WWOX Gene Influences Cellular Pathways in the Neuronal Differentiation of Human Neural Progenitor Cells.

    abstract::The brain is the most functionally organized structure of all organs. It manages behavior, perception and higher cognitive functions. The WWOX gene is non-classical tumor suppressor gene, which has been shown to have an impact on proliferation, apoptosis and migration processes. Moreover, genetic aberrations in WWOX i...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2019.00391

    authors: Kośla K,Płuciennik E,Styczeń-Binkowska E,Nowakowska M,Orzechowska M,Bednarek AK

    更新日期:2019-08-30 00:00:00

  • Plasticity of GABA transporters: an unconventional route to shape inhibitory synaptic transmission.

    abstract::The brain relies on GABAergic neurons to control the ongoing activity of neuronal networks. GABAergic neurons control the firing pattern of excitatory cells, the temporal structure of membrane potential oscillations and the time window for integration of synaptic inputs. These actions require a fine control of the tim...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2014.00128

    authors: Scimemi A

    更新日期:2014-05-13 00:00:00

  • 2-Methyl-6-(phenylethynyl) pyridine (MPEP) reverses maze learning and PSD-95 deficits in Fmr1 knock-out mice.

    abstract::Fragile X Syndrome (FXS) is caused by the lack of expression of the fragile X mental retardation protein (FMRP), which results in intellectual disability and other debilitating symptoms including impairment of visual-spatial functioning. FXS is the only single-gene disorder that is highly co-morbid with autism spectru...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2014.00070

    authors: Gandhi RM,Kogan CS,Messier C

    更新日期:2014-03-06 00:00:00

  • S100B Impairs Oligodendrogenesis and Myelin Repair Following Demyelination Through RAGE Engagement.

    abstract::Increased expression of S100B and its specific receptor for advanced glycation end products (RAGE) has been described in patients with multiple sclerosis (MS), being associated with an active demyelinating process. We previously showed that a direct neutralization of S100B reduces lysophosphatidylcholine (LPC)-induced...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2020.00279

    authors: Santos G,Barateiro A,Brites D,Fernandes A

    更新日期:2020-09-04 00:00:00

  • ER stress and unfolded protein response in amyotrophic lateral sclerosis-a controversial role of protein disulphide isomerase.

    abstract::Accumulation of proteins in aberrant conformation occurs in many neurodegenerative diseases. Furthermore, dysfunctions in protein handling in endoplasmic reticulum (ER) and the following ER stress have been implicated in a vast number of diseases, such as amyotrophic lateral sclerosis (ALS). During excessive ER stress...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章,评审

    doi:10.3389/fncel.2014.00402

    authors: Jaronen M,Goldsteins G,Koistinaho J

    更新日期:2014-12-02 00:00:00

  • Suppression of piriform cortex activity in rat by corticotropin-releasing factor 1 and serotonin 2A/C receptors.

    abstract::The piriform cortex (PC) is richly innervated by corticotropin-releasing factor (CRF) and serotonin (5-HT) containing axons arising from central amygdala and Raphe nucleus. CRFR1 and 5-HT2A/2CRs have been shown to interact in manner where CRFR activation subsequently potentiates the activity of 5-HT2A/2CRs. The purpos...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2015.00200

    authors: Narla C,Dunn HA,Ferguson SS,Poulter MO

    更新日期:2015-05-28 00:00:00

  • IL-33/ST2L Signaling Provides Neuroprotection Through Inhibiting Autophagy, Endoplasmic Reticulum Stress, and Apoptosis in a Mouse Model of Traumatic Brain Injury.

    abstract::Interleukin-33 (IL-33) is a member of the interleukin-1 (IL-1) cytokine family and an extracellular ligand for the orphan IL-1 receptor ST2. Accumulated evidence shows that the IL-33/ST2 axis plays a crucial role in the pathogenesis of central nervous system (CNS) diseases and injury, including traumatic brain injury ...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2018.00095

    authors: Gao Y,Zhang MY,Wang T,Fan YY,Yu LS,Ye GH,Wang ZF,Gao C,Wang HC,Luo CL,Tao LY

    更新日期:2018-04-25 00:00:00

  • NKCC1-Deficiency Results in Abnormal Proliferation of Neural Progenitor Cells of the Lateral Ganglionic Eminence.

    abstract::The proliferative pool of neural progenitor cells is maintained by exquisitely controlled mechanisms for cell cycle regulation. The Na-K-Cl cotransporter (NKCC1) is important for regulating cell volume and the proliferation of different cell types in vitro. NKCC1 is expressed in ventral telencephalon of embryonic brai...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2016.00200

    authors: Magalhães AC,Rivera C

    更新日期:2016-08-17 00:00:00

  • Structural and Functional Features of Developing Brain Capillaries, and Their Alteration in Schizophrenia.

    abstract::Schizophrenia affects more than 1% of the world's population and shows very high heterogeneity in the positive, negative, and cognitive symptoms experienced by patients. The pathogenic mechanisms underlying this neurodevelopmental disorder are largely unknown, although it is proposed to emerge from multiple genetic an...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章,评审

    doi:10.3389/fncel.2020.595002

    authors: Carrier M,Guilbert J,Lévesque JP,Tremblay MÈ,Desjardins M

    更新日期:2021-01-15 00:00:00

  • Effect of histone deacetylase inhibitors trichostatin A and valproic acid on hair cell regeneration in zebrafish lateral line neuromasts.

    abstract::In humans, auditory hair cells are not replaced when injured. Thus, cochlear hair cell loss causes progressive and permanent hearing loss. Conversely, non-mammalian vertebrates are capable of regenerating lost sensory hair cells. The zebrafish lateral line has numerous qualities that make it well-suited for studying h...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2014.00382

    authors: He Y,Cai C,Tang D,Sun S,Li H

    更新日期:2014-11-13 00:00:00

  • Local mechanisms for loud sound-enhanced aminoglycoside entry into outer hair cells.

    abstract::Loud sound exposure exacerbates aminoglycoside ototoxicity, increasing the risk of permanent hearing loss and degrading the quality of life in affected individuals. We previously reported that loud sound exposure induces temporary threshold shifts (TTS) and enhances uptake of aminoglycosides, like gentamicin, by cochl...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2015.00130

    authors: Li H,Kachelmeier A,Furness DN,Steyger PS

    更新日期:2015-04-14 00:00:00

  • Molecular Pathology and Pharmacological Treatment of Autism Spectrum Disorder-Like Phenotypes Using Rodent Models.

    abstract::Autism spectrum disorder (ASD) is a heterogeneous neurodevelopmental disorder with a high prevalence rate. The core symptoms of ASD patients are impaired social communication and repetitive behavior. Genetic and environmental factors contribute to pathophysiology of ASD. Regarding environmental risk factors, it is kno...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章,评审

    doi:10.3389/fncel.2018.00422

    authors: Kuo HY,Liu FC

    更新日期:2018-11-20 00:00:00

  • The Molecular Determinants of Mitochondrial Membrane Contact With ER, Lysosomes and Peroxisomes in Neuronal Physiology and Pathology.

    abstract::Membrane tethering is an important communication method for membrane-packaged organelles. Mitochondria are organelles with a bilayer membrane, and the membrane contact between mitochondria and other organelles is indispensable for maintaining cellular homeostasis. Increased levels of molecular determinants that mediat...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2020.00194

    authors: Liao Y,Dong Y,Cheng J

    更新日期:2020-08-07 00:00:00

  • Human Neural Stem Cell Induced Functional Network Stabilization After Cortical Stroke: A Longitudinal Resting-State fMRI Study in Mice.

    abstract::Most stroke studies dealing with functional deficits and assessing stem cell therapy produce extensive hemispheric damage and can be seen as a model for severe clinical strokes. However, mild strokes have a better prospect for functional recovery. Recently, anatomic and behavioral changes have been reported for distal...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2020.00086

    authors: Minassian A,Green C,Diedenhofen M,Vogel S,Hess S,Stoeber M,Radmilovic MD,Wiedermann D,Kloppenburg P,Hoehn M

    更新日期:2020-04-07 00:00:00

  • Selective Modulation of α5 GABAA Receptors Exacerbates Aberrant Inhibition at Key Hippocampal Neuronal Circuits in APP Mouse Model of Alzheimer's Disease.

    abstract::Selective negative allosteric modulators (NAMs), targeting α5 subunit-containing GABAA receptors (GABAARs) as potential therapeutic targets for disorders associated with cognitive deficits, including Alzheimer's disease (AD), continually fail clinical trials. We investigated whether this was due to the change in the e...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2020.568194

    authors: Petrache AL,Khan AA,Nicholson MW,Monaco A,Kuta-Siejkowska M,Haider S,Hilton S,Jovanovic JN,Ali AB

    更新日期:2020-11-11 00:00:00

  • Different transporter systems regulate extracellular GABA from vesicular and non-vesicular sources.

    abstract::Tonic GABA type A (GABAA) conductance is a key factor regulating neuronal excitability and computation in neuronal networks. The magnitude of the tonic GABAA conductance depends on the concentration of ambient GABA originating from vesicular and non-vesicular sources and is tightly regulated by GABA uptake. Here we sh...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2013.00023

    authors: Song I,Volynski K,Brenner T,Ushkaryov Y,Walker M,Semyanov A

    更新日期:2013-03-13 00:00:00

  • Pannexin 1 Modulates Axonal Growth in Mouse Peripheral Nerves.

    abstract::The pannexin family of channels consists of three members-pannexin-1 (Panx1), pannexin-2 (Panx2), and pannexin-3 (Panx3) that enable the exchange of metabolites and signaling molecules between intracellular and extracellular compartments. Pannexin-mediated release of intracellular ATP into the extracellular space has ...

    journal_title:Frontiers in cellular neuroscience

    pub_type: 杂志文章

    doi:10.3389/fncel.2017.00365

    authors: Horton SM,Luna Lopez C,Blevins E,Howarth H,Weisberg J,Shestopalov VI,Makarenkova HP,Shah SB

    更新日期:2017-11-22 00:00:00