Abstract:
BACKGROUND:Epigenetics changes have been shown to be affected by cigarette smoking. Cigarette smoke (CS)-mediated DNA methylation can potentially affect several cellular and pathophysiological processes, acute exacerbations, and comorbidity in the lungs of patients with chronic obstructive pulmonary disease (COPD). We sought to determine whether genome-wide lung DNA methylation profiles of smokers and patients with COPD were significantly different from non-smokers. We isolated DNA from parenchymal lung tissues of patients including eight lifelong non-smokers, eight current smokers, and eight patients with COPD and analyzed the samples using Illumina's Infinium HumanMethylation450 BeadChip. RESULTS:Our data revealed that the differentially methylated genes were related to top canonical pathways (e.g., G beta gamma signaling, mechanisms of cancer, and nNOS signaling in neurons), disease and disorders (organismal injury and abnormalities, cancer, and respiratory disease), and molecular and cellular functions (cell death and survival, cellular assembly and organization, cellular function and maintenance) in patients with COPD. The genome-wide DNA methylation analysis identified suggestive genes, such as NOS1AP, TNFAIP2, BID, GABRB1, ATXN7, and THOC7 with DNA methylation changes in COPD lung tissues that were further validated by pyrosequencing. Pyrosequencing validation confirmed hyper-methylation in smokers and patients with COPD as compared to non-smokers. However, we did not detect significant differences in DNA methylation for TNFAIP2, ATXN7, and THOC7 genes in smokers and COPD groups despite the changes observed in the genome-wide analysis. CONCLUSIONS:Our study suggests that DNA methylation in suggestive genes, such as NOS1AP, BID, and GABRB1 may be used as epigenetic signatures in smokers and patients with COPD if the same is validated in a larger cohort. Future studies are required to correlate DNA methylation status with transcriptomics of selective genes identified in this study and elucidate their role and involvement in the progression of COPD and its exacerbations.
journal_name
Clin Epigeneticsjournal_title
Clinical epigeneticsauthors
Sundar IK,Yin Q,Baier BS,Yan L,Mazur W,Li D,Susiarjo M,Rahman Idoi
10.1186/s13148-017-0335-5subject
Has Abstractpub_date
2017-04-14 00:00:00pages
38eissn
1868-7075issn
1868-7083pii
335journal_volume
9pub_type
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-017-0434-3
更新日期:2018-01-04 00:00:00
abstract:Background:Maternal social environmental stressors during pregnancy are associated with adverse birth and child developmental outcomes, and epigenetics has been proposed as a possible mechanism for such relationships. Methods:In a Mexican-American birth cohort of 241 maternal-infant pairs, cord blood samples were meas...
journal_title:Clinical epigenetics
pub_type: 杂志文章
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更新日期:2018-05-08 00:00:00
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更新日期:2018-10-24 00:00:00
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journal_title:Clinical epigenetics
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journal_title:Clinical epigenetics
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journal_title:Clinical epigenetics
pub_type: 临床试验,杂志文章
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更新日期:2019-10-11 00:00:00
abstract:INTRODUCTION:DNA methylation of CpG islands within the promoter region of genes is an epigenetic modification with an important role in the development of cancer and it is typically mediated by DNA methyltransferases (DNMTs). In cancer cells, global hypomethylation of the genome as a whole and regional hypermethylation...
journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/1868-7083-5-7
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-017-0421-8
更新日期:2017-11-07 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-015-0076-2
更新日期:2015-04-10 00:00:00
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journal_title:Clinical epigenetics
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-020-00832-6
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abstract:BACKGROUND:The current epidemic of obesity and associated diseases calls for swift actions to better understand the mechanisms by which genetics and environmental factors affect metabolic health in humans. Monozygotic (MZ) twin pairs showing discordance for obesity suggest that epigenetic influences represent one such ...
journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-015-0073-5
更新日期:2015-04-02 00:00:00
abstract::Altered DNA methylation events contribute to the pathogenesis and progression of metabolic disorders, including nonalcoholic fatty liver disease (NAFLD). Investigations of global DNA methylation patterns in liver biopsies representing severe NAFLD fibrosis have been limited. We used the HumanMethylation 450K BeadChip ...
journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-018-0525-9
更新日期:2018-07-13 00:00:00
abstract:BACKGROUND:Frozen-thawed embryo transfer (FET) is increasingly available for the improvement of the success rate of assisted reproductive technologies other than fresh embryo transfer (ET). There have been numerous findings that FET provides better obstetric and perinatal outcomes. However, the birth weight of infants ...
journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-017-0379-6
更新日期:2017-08-03 00:00:00
abstract:BACKGROUND:Inflammation has been associated with higher rates of recurrence and mortality in head and neck cancer (HNC). While the biological mechanisms predisposing patients to heightened inflammatory states remain largely unknown, DNA methylation has been proposed to reflect systemic inflammation. In this analysis, w...
journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-020-00930-5
更新日期:2020-09-11 00:00:00
abstract:BACKGROUND:Zinc-finger protein 471 (ZNF471) is a member of the Krüppel-associated box domain zinc finger protein (KRAB-ZFP) family. ZNF471 is methylated in squamous cell carcinomas of tongue, stomach and esophageal. However, its role in breast carcinogenesis remains elusive. Here, we studied its expression, functions, ...
journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-020-00959-6
更新日期:2020-11-17 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-018-0557-1
更新日期:2018-11-01 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-016-0185-6
更新日期:2016-02-19 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-016-0195-4
更新日期:2016-03-15 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
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更新日期:2011-12-05 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
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更新日期:2021-01-25 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-020-00970-x
更新日期:2020-11-23 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1007/s13148-011-0038-2
更新日期:2011-08-01 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/1868-7083-5-18
更新日期:2013-10-03 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-020-0824-9
更新日期:2020-02-19 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-015-0152-7
更新日期:2015-11-05 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-018-0515-y
更新日期:2018-06-20 00:00:00
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journal_title:Clinical epigenetics
pub_type: 临床试验,杂志文章
doi:10.1186/s13148-019-0642-0
更新日期:2019-03-15 00:00:00
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journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-020-00989-0
更新日期:2021-01-29 00:00:00
abstract:BACKGROUND:Many conventional chemotherapeutic drugs are known to be involved in DNA damage, thus ultimately leading to apoptosis of leukemic cells. However, they fail to completely eliminate leukemia stem cells (LSCs) due to their higher DNA repair capacity of cancer stem cells than that of bulk cancer cells, which bec...
journal_title:Clinical epigenetics
pub_type: 杂志文章
doi:10.1186/s13148-017-0377-8
更新日期:2017-08-14 00:00:00