Abstract:
:Significant progress has been made in the last decade in the development of optogenetic effectors and sensors that can be deployed to understand complex biological signaling in mammals at a molecular level, without disrupting the distributed, lineage specific signaling circuits that comprise nuanced physiological responses. A major barrier to the widespread exploitation of these imaging tools, however, is the lack of readily available genetic reagents that can be easily combined to probe complex biological processes. Ideally, one could envision purpose-produced mouse lines expressing optically compatible sensors and effectors, sensor pairs in distinct lineages, or sensor pairs in discrete subcellular compartments, such that they could be crossed to enable in vivo imaging studies of unprecedented scientific power. Such lines could also be combined with mice to determine the alteration in signaling accompanying targeted gene deletion or addition. In order to address this lack, the National Heart Lung and Blood Institute has recently funded an optogenetic resource designed to create optically compatible, combinatorial mouse lines that will advance NHLBI research. Here we review recent advances in optogenetic sensor and effectors and describe the rationale and goals for the establishment of the Cornell/National Heart Lung Blood Resource for Optogenetic Mouse Signaling (CHROMus).
journal_name
Front Physioljournal_title
Frontiers in physiologyauthors
Shui B,Lee JC,Reining S,Lee FK,Kotlikoff MIdoi
10.3389/fphys.2014.00428subject
Has Abstractpub_date
2014-11-06 00:00:00pages
428issn
1664-042Xjournal_volume
5pub_type
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journal_title:Frontiers in physiology
pub_type: 杂志文章
doi:10.3389/fphys.2016.00211
更新日期:2016-06-07 00:00:00
abstract::Due to the important biological role of red blood cells (RBCs) in vertebrates, the analysis of reshaping and dynamics of RBCs motion is a critical issue in physiology and biomechanics. In this paper the behavior of RBCs within the immature capillary plexus during embryonic development of zebrafish has been analyzed. R...
journal_title:Frontiers in physiology
pub_type: 杂志文章
doi:10.3389/fphys.2016.00455
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abstract::Periodontal ligament contains periodontal ligament stem cells that maintain tissue homeostasis. Targeting hPDLSCs (human periodontal ligament cells) is a promising strategy for repair and regeneration of bone tissue destroyed by periodontal diseases. However, the mechanisms by which PDLSCs differentiate into osteoblas...
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pub_type: 杂志文章
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journal_title:Frontiers in physiology
pub_type: 杂志文章
doi:10.3389/fphys.2017.00907
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abstract::Trace conditioning is a form of associative learning that can be induced by presenting a conditioned stimulus (CS) and an unconditioned stimulus (US) following each other, but separated by a temporal gap. This gap distinguishes trace conditioning from classical delay conditioning, where the CS and US overlap. To bridg...
journal_title:Frontiers in physiology
pub_type: 杂志文章
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abstract::Claudin-1 is a tight junction transmembrane protein. Its localization in the maturation ameloblasts of rat incisors was examined by immunofluorescence microscopy. Distal junction area of ruffle-ended ameloblasts (RA) and the Golgi apparatus of a sub-population of smooth-ended ameloblasts (SA) and RAs stained positive ...
journal_title:Frontiers in physiology
pub_type: 杂志文章
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abstract::Genetically modified mice provide a number of models for studying cardiac channelopathies related to cardiac Na(+) channel (SCN5A) abnormalities. We review key pathophysiological features in these murine models that may underlie clinical features observed in sinus node dysfunction and progressive cardiac conduction di...
journal_title:Frontiers in physiology
pub_type: 杂志文章
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journal_title:Frontiers in physiology
pub_type: 杂志文章,评审
doi:10.3389/fphys.2017.00614
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abstract::The bicuspid aortic valve, a kind of heart disease that comes from parents, has been paid attention around the world. Although most bicuspid aortic valve (BAV) patients will suffer from some complications including aortic stenosis, aortic regurgitation, endocarditis, and heart dysfunction in the late stage of the dise...
journal_title:Frontiers in physiology
pub_type: 杂志文章,评审
doi:10.3389/fphys.2018.01921
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abstract::Ameloblasts are ectoderm-derived cells that produce an extracellular enamel matrix that mineralizes to form enamel. The development and use of immortalized cell lines, with a stable phenotype, is an important contribution to biological studies as it allows for the investigation of molecular activities without the cont...
journal_title:Frontiers in physiology
pub_type: 杂志文章
doi:10.3389/fphys.2014.00277
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abstract::The ultrarapid delayed rectifier K+ current (IKur), mediated by Kv1.5 channels, constitutes a key component of the atrial action potential. Functional mutations in the underlying KCNA5 gene have been shown to cause hereditary forms of atrial fibrillation (AF). Here, we combine targeted genetic engineering with cardiac...
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pub_type: 杂志文章
doi:10.3389/fphys.2017.00469
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journal_title:Frontiers in physiology
pub_type: 杂志文章,评审
doi:10.3389/fphys.2020.00661
更新日期:2020-06-16 00:00:00
abstract::Ischemia/reperfusion (I/R) injury induces irreversible oxidative stress damage to the cardiac myocytes. Many studies have revealed that propofol alleviates the important organelle-mediated injury from oxidative stress in vitro. However, it remains unclear whether propofol prevents I/R-induced DNA damage in cardiomyocy...
journal_title:Frontiers in physiology
pub_type: 杂志文章
doi:10.3389/fphys.2019.00223
更新日期:2019-03-15 00:00:00
abstract::Restitution of cutaneous sensory function is accomplished by neural regenerative processes of distinct mechanisms following peripheral nerve lesions. Although methods available for the study of functional cutaneous nerve regeneration are specific and accurate, they are unsuitable for the longitudinal follow-up of the ...
journal_title:Frontiers in physiology
pub_type: 杂志文章
doi:10.3389/fphys.2020.00439
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abstract::Osteoporosis is a major health problem in post-menopausal women (PMW). Exercise training is considered a cost-effective strategy to prevent osteoporosis in middle aged-older people. The purpose of this study is to summarize the effect of exercise on BMD among PMW. A comprehensive search of electronic databases was con...
journal_title:Frontiers in physiology
pub_type:
doi:10.3389/fphys.2020.00652
更新日期:2020-06-23 00:00:00
abstract::Mucolipins (TRPML) are endosome/lysosome Ca2+ permeable channels belonging to the family of transient receptor potential channels. In mammals, there are three TRPML proteins, TRPML1, 2, and 3, encoded by MCOLN1-3 genes. Among these channels, TRPML1 is a reactive oxygen species sensor localized on the lysosomal membran...
journal_title:Frontiers in physiology
pub_type: 杂志文章,评审
doi:10.3389/fphys.2020.00251
更新日期:2020-03-24 00:00:00
abstract::Obesity is a multi-factor chronic disease caused by the mixed influence of genetics, environments and an imbalance of energy intake and expenditure. Due to lifestyle changes, modern society sees a rapid increase in obesity occurrence along with an aggravated risk of metabolic syndromes in the general population, inclu...
journal_title:Frontiers in physiology
pub_type: 杂志文章,评审
doi:10.3389/fphys.2018.00850
更新日期:2018-07-06 00:00:00
abstract::The RR-interval time series or tachograms obtained from electrocardiograms have been widely studied since they reflect the cardiac variability, and this is an indicative of the health status of a person. The tachogram can be seen as a highly non-linear and complex time series, and therefore, should be analyzed with no...
journal_title:Frontiers in physiology
pub_type: 杂志文章
doi:10.3389/fphys.2020.00981
更新日期:2020-08-12 00:00:00
abstract::The ubiquitin proteasome system (UPS) is a highly regulated mechanism of intracellular protein degradation and turnover. To evaluate the effect of crowding stress on the UPS in fish, grass carp (Ctenopharyngodon idellus) were randomly reared at low stocking density (LSD, 0.9 kg m-2) or high stocking density (HSD, 5.9 ...
journal_title:Frontiers in physiology
pub_type: 杂志文章
doi:10.3389/fphys.2018.00837
更新日期:2018-07-05 00:00:00
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pub_type: 杂志文章
doi:10.3389/fphys.2019.00577
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journal_title:Frontiers in physiology
pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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journal_title:Frontiers in physiology
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journal_title:Frontiers in physiology
pub_type: 杂志文章
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journal_title:Frontiers in physiology
pub_type: 杂志文章
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journal_title:Frontiers in physiology
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