Abstract:
:In addition to its traditional role in the regulation of calcium homeostasis and bone metabolism, vitamin D also exhibits immunomodulatory, anti-proliferative and cancer preventive activities. Molecular mechanisms that confer the chemo-preventive properties to vitamin D are poorly understood. We previously reported that constitutive phosphorylation of histone H2AX on Ser139 (γH2AX) and activation of ATM (Ser1981 phosphorylation), seen in untreated normal or tumor cells predominantly in S phase of the cell cycle, is to a large extent indicative of DNA replication stress occurring as a result of persistent DNA damage caused by endogenous oxidants, by-products of oxidative metabolism. In the present study we observed that exposure of mitogenically stimulated human lymphocytes, pulmonary carcinoma A549 and lymphoblastoid TK6 cells to 1,25-dihydroxyvitamin D3 (1,25-VD) reduced the level of constitutive expression of γH2AX and ATM-S1981P. We also observed that the H2O2-induced rise in the level of γH2AX in lymphocytes was attenuated by 1,25-VD. Whereas in lymphocytes 1,25-VD reduced by 50-70% the level of endogenous oxidants as determined by their ability to oxidize 2,7-dichlorodihydrofluorescein (DCFH) in A549 and TK6 cells the attenuation of DNA damage signaling by 1,25-VD was seen in the absence of detectable reduction in DCFH oxidation. These findings suggest that while the anti-oxidant activity of 1,25-VD may contribute to a reduction in the intensity of DNA replication stress in lymphocytes, other factors play a role in the 1,25-VD effects seen in A549 and TK6 cells. The data are consistent with the recent report on the interaction between DNA damage signaling (ATM activation) and 1,25D receptor (VDR) phosphorylation that lead to enhancement of DNA repair efficiency, and provide further support for the chemo-preventive and anti-aging properties of this vitamin/hormone.
journal_name
Aging (Albany NY)journal_title
Agingauthors
Halicka HD,Zhao H,Li J,Traganos F,Studzinski GP,Darzynkiewicz Zdoi
10.18632/aging.100450subject
Has Abstractpub_date
2012-04-01 00:00:00pages
270-8issue
4issn
1945-4589pii
100450journal_volume
4pub_type
杂志文章相关文献
Aging-US文献大全abstract::Despite improvements in surgical procedures and comprehensive therapies, pancreatic cancer remains one of the most aggressive and deadly human malignancies. It is therefore necessary to determine which cellular mediators associate with prognosis in pancreatic cancer so as to improve the treatment of this disease. In t...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102096
更新日期:2019-07-21 00:00:00
abstract::Cybrid technology was used to replace Leber hereditary optic neuropathy (LHON) causing mitochondrial DNA (mtDNA) mutations from patient-specific fibroblasts with wildtype mtDNA, and mutation-free induced pluripotent stem cells (iPSCs) were generated subsequently. Retinal ganglion cell (RGC) differentiation demonstrate...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101231
更新日期:2017-04-01 00:00:00
abstract::Caveolae are vesicular invaginations of the plasma membrane. Caveolin-1 is the structural protein component of caveolae. Caveolin-1 participates in signal transduction processes by acting as a scaffolding protein that concentrates, organizes and functional regulates signaling molecules within caveolar membranes. Cigar...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100079
更新日期:2009-08-10 00:00:00
abstract::As the outbreak of coronavirus disease 2019 (COVID-19) progresses, prognostic markers for early identification of high-risk individuals are an urgent medical need. Italy has one of the highest numbers of SARS-CoV-2-related deaths and one of the highest mortality rates. Worldwide, a more severe course of COVID-19 is as...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103415
更新日期:2020-06-05 00:00:00
abstract::Chondroitin sulfate (CS) proteoglycans in perineuronal nets (PNNs) from the central nervous system (CNS) are involved in the control of plasticity and memory. Removing PNNs reactivates plasticity and restores memory in models of Alzheimer's disease and ageing. Their actions depend on the glycosaminoglycan (GAG) chains...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101256
更新日期:2017-06-28 00:00:00
abstract::In this meta-analysis, we systematically investigated the correlation between single nucleotide polymorphisms (SNPs) and pancreatic cancer (PC) risk. We searched PubMed, Network Science, EMBASE, Cochrane Library, China National Knowledge Infrastructure (CNKI), China Science and Technology Periodical Database (VIP), an...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104128
更新日期:2020-11-20 00:00:00
abstract::New strategies to block the immune evasion activity of programmed death ligand-1 (PD-L1) are urgently needed. When exploring the PD-L1-targeted effects of mechanistically diverse metabolism-targeting drugs, exposure to the dietary polyphenol resveratrol (RSV) revealed its differential capacity to generate a distinct P...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102646
更新日期:2020-01-04 00:00:00
abstract::Aging and age-related diseases occur in almost all organisms. Recently, it was discovered that the inhibition of target of rapamycin complex 1 (TORC1), a conserved complex that mediates nutrient status and cell metabolism, can extend an individual's lifespan and inhibit age-related diseases in many model organisms. Ho...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102428
更新日期:2019-11-11 00:00:00
abstract::Age-related loss of muscle mass and strength can be accelerated by impaired recovery of muscle mass following a transient atrophic stimulus. The aim of this study was to identify the mechanisms underlying the attenuated recovery of muscle mass and strength in old rats following disuse-induced atrophy. Adult (9 month) ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100879
更新日期:2016-01-01 00:00:00
abstract::Progressive loss of ovarian estrogen (E2) production is a hallmark feature of, if not a driving force behind, reproductive aging and the menopause. Recent genetic studies in mice have shown that female germline or oogonial stem cells (OSCs) contribute to maintenance of adult ovarian function and fertility under physio...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103080
更新日期:2020-04-17 00:00:00
abstract::Numerous studies suggest that rapamycin treatment promotes insulin resistance, implying that rapamycin could have negative effects on patients with, or at risk for, type 2 diabetes (T2D). New evidence, however, indicates that rapamycin treatment produces some benefits to energy metabolism, even in the context of T2D. ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101117
更新日期:2016-11-30 00:00:00
abstract::MicroRNAs (miRNAs) serve as regulatory factors in both healthy tissue and various cancers. Here, we used an miRNA microarray to screen for miRNAs differentially expressed between HNSCC and adjacent epithelial tissue. Among these, levels of miR-141 were significantly reduced in HNSCC tissues. Expression levels of epide...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101791
更新日期:2019-02-08 00:00:00
abstract::Salivary gland dysfunction is a common symptom that occurs after menopause. This study was performed to investigate the mechanism of salivary gland dysfunction to confirm the relationship between ferroptosis and salivary gland dysfunction by ovariectomy. Forty-eight female rats were randomly divided into four groups (...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103882
更新日期:2020-11-05 00:00:00
abstract::The main aspects of severe COVID-19 disease pathogenesis include hyper-induction of proinflammatory cytokines, also known as 'cytokine storm', that precedes acute respiratory distress syndrome (ARDS) and often leads to death. COVID-19 patients often suffer from lung fibrosis, a serious and untreatable condition. There...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.202500
更新日期:2021-01-19 00:00:00
abstract::Cellular senescence is considered a stress response imposing a stable cell cycle arrest to restrict the growth of damaged cells. More recently however, cellular senescence was identified during mouse embryo development at particular structures during specific periods of time. This programmed cell senescence has been p...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103968
更新日期:2020-09-29 00:00:00
abstract::The broccoli-derived isothiocyanate sulforaphane inhibits inflammation, oxidative stress and cancer, but its effect on healthspan and longevity are unclear. We used the C. elegans nematode model and fed the wildtype and 9 mutant strains ±sulforaphane. The lifespan, phenotype, pharyngeal pumping, mobility, lipofuscin a...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.202512
更新日期:2021-01-20 00:00:00
abstract::Compounds that delay aging in model organisms may be of significant interest to anti-aging medicine, since these substances potentially provide pharmaceutical approaches to promote healthy lifespan in humans. We here aimed to test whether pharmaceutical concentrations of three fibrates, pharmacologically established s...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100548
更新日期:2013-04-01 00:00:00
abstract::Programmed death-1 (PD-1) polymorphisms have been associated with esophageal cancer risk. Here, the aims of this case-control study were to explore whether three PD-1 polymorphisms (rs10204525, rs7421861, and rs36084323) were related with the risk and clinical features of esophageal cancer in Chinese Han subjects (n =...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102845
更新日期:2020-02-21 00:00:00
abstract:BACKGROUND:Chromosome 1p/19q codeletion is increasingly being recognized as the crucial genetic marker for glioma patients and have been included in WHO classification of glioma in 2016. Fluorescent in situ hybridization, a widely used method in detecting 1p/19q status, has some methodological limitations which might i...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101795
更新日期:2019-02-02 00:00:00
abstract::Increased activation of catabolic pathways, including apoptosis causes sarcopenia. However, the precise molecular mechanism that initiates apoptosis during aging is not well understood. Here, we report that aging alters miRNA expression profile in mouse skeletal muscle as evidenced by miRNA microarray and real-time PC...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101207
更新日期:2017-03-22 00:00:00
abstract::As of May 5, 2020, the number of confirmed coronavirus disease (COVID-19) cases has been more than 3.5 million with 243,540 deaths. We aimed to determine the associations between ageing population, median age, life expectancy at birth and COVID-19 mortality. The numbers of COVID-19 cases and deaths in the European reg...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104193
更新日期:2020-11-24 00:00:00
abstract::There are no specific therapies for autosomal dominant polycystic kidney disease (ADPKD), and clinical data evaluating the effects of non-specific therapies on ADPKD patients are scarce. We therefore evaluated those effects using data from a longitudinal health insurance database collected from 2000-2010. We individua...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102858
更新日期:2020-02-24 00:00:00
abstract::There is a pressing need to identify markers of cognitive and neural decline in healthy late-midlife participants. We explored the relationship between cross-sectional structural brain-imaging derived phenotypes (IDPs) and cognitive ability, demographic, health and lifestyle factors (non-IDPs). Participants were recru...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102151
更新日期:2019-08-26 00:00:00
abstract::Patients with end-stage renal disease (ESRD) display phenotypic features of premature biological aging, characterized by disproportionately high morbidity and mortality at a younger age. Nuclear factor erythroid 2-related factor 2 (Nrf2) activity, a master regulator of antioxidative responses, declines with age and is...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103685
更新日期:2020-07-13 00:00:00
abstract::Epigenetic regulation of various genomic functions, including gene expression, provide mechanisms whereby an organism can dynamically respond to changes in its environment and modify gene expression accordingly. One epigenetic mechanism implicated in human aging and age-related disorders is DNA methylation. Isolated p...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101187
更新日期:2017-02-28 00:00:00
abstract::Proinsulin, insulin and proinsulin/insulin (P/I) ratio have been reported to be correlated with fasting plasma glucose (FPG) and Hemoglobin A1c (HbA1c) in whole population study therefore sensitive predictors of T2D progression. However, by analyzing data collected from 2018-2019 from a cohort of 1579 East Asian indiv...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103289
更新日期:2020-06-12 00:00:00
abstract::Inflammatory bowel disease (IBD) is a group of chronic and recurrent nonspecific inflammatory disorders, including Crohn's disease (CD) and ulcerative colitis (UC). Due to the persistent inflammation of intestinal mucosa caused by immune disorders, barrier dysfunction may be an essential cause of the pathogenesis of I...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103566
更新日期:2020-08-28 00:00:00
abstract::Senescent cells secrete inflammatory cytokines, proteases, and other factors, which are indicated as senescence-associated secretory phenotype (SASP). There are contrasting studies on the role of the SASP in cancer. Studies suggested that cancer cells may misuse the senescent secretome for their growth. Other investig...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102172
更新日期:2019-08-14 00:00:00
abstract:OBJECTIVE:This study aimed to explore the effect of silencing hTERT on the CSC-like characteristics and radioresistance of CNE-2R cells. RESULTS:Silencing hTERT suppressed CNE-2R cell proliferation and increased the cell apoptosis rate and radiosensitivity in vitro. Moreover, it could also inhibit the growth of xenogr...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104167
更新日期:2020-11-24 00:00:00
abstract::Age-related hearing loss (ARHL) has been considered as a promising modifiable risk factor for cognitive impairment and dementia. Nonetheless, it is still unclear whether age-related hearing loss associates with neurodegenerative biomarkers of Alzheimer's disease (AD). Participants with ARHL were selected from the esta...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101971
更新日期:2019-05-22 00:00:00