Abstract:
:The leptospiral LigA protein consists of 13 bacterial immunoglobulin-like (Big) domains and is the only purified recombinant subunit vaccine that has been demonstrated to protect against lethal challenge by a clinical isolate of Leptospira interrogans in the hamster model of leptospirosis. We determined the minimum number and location of LigA domains required for immunoprotection. Immunization with domains 11 and 12 was found to be required but insufficient for protection. Inclusion of a third domain, either 10 or 13, was required for 100% survival after intraperitoneal challenge with Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130. As in previous studies, survivors had renal colonization; here, we quantitated the leptospiral burden by qPCR to be 1.2×10(3) to 8×10(5) copies of leptospiral DNA per microgram of kidney DNA. Although renal histopathology in survivors revealed tubulointerstitial changes indicating an inflammatory response to the infection, blood chemistry analysis indicated that renal function was normal. These studies define the Big domains of LigA that account for its vaccine efficacy and highlight the need for additional strategies to achieve sterilizing immunity to protect the mammalian host from leptospiral infection and its consequences.
journal_name
PLoS Negl Trop Disjournal_title
PLoS neglected tropical diseasesauthors
Coutinho ML,Choy HA,Kelley MM,Matsunaga J,Babbitt JT,Lewis MS,Aleixo JA,Haake DAdoi
10.1371/journal.pntd.0001422subject
Has Abstractpub_date
2011-12-01 00:00:00pages
e1422issue
12eissn
1935-2727issn
1935-2735pii
PNTD-D-11-00830journal_volume
5pub_type
杂志文章abstract::Burkholderia pseudomallei, the bacterial agent of melioidosis, causes disease through inhalation of infectious particles, and is classified as a Tier 1 Select Agent. Optical diagnostic imaging has demonstrated that murine respiratory disease models are subject to significant upper respiratory tract (URT) colonization....
journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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abstract:BACKGROUND:Lymphatic filariasis (LF), a global public health problem affecting approximately 120 million people worldwide, is a leading cause of disability in the developing world including the South Pacific. Despite decades of ongoing mass drug administration (MDA) in the region, some island nations have not yet achie...
journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章,评审
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
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doi:10.1371/journal.pntd.0005697
更新日期:2017-08-04 00:00:00
abstract:INTRODUCTION:Schistosomiasis is a chronic parasitic trematode disease that affects over 240 million people worldwide. The Schistosoma lifecycle is complex, involving transmission via specific intermediate-host freshwater snails. Predictive mathematical models of Schistosoma transmission have often chosen to simplify or...
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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更新日期:2018-03-26 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
doi:10.1371/journal.pntd.0006220
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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abstract::[This corrects the article DOI: 10.1371/journal.pntd.0007025.]. ...
journal_title:PLoS neglected tropical diseases
pub_type: 已发布勘误
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journal_title:PLoS neglected tropical diseases
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pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章,meta分析,评审
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更新日期:2014-11-13 00:00:00
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pub_type: 杂志文章
doi:10.1371/journal.pntd.0000854
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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更新日期:2015-10-06 00:00:00