Abstract：
:Molecular monitoring of minimal residual disease (MRD) and BCR-ABL1 kinase domain (KD) mutation testing have a well consolidated role in the routine management of chronic myeloid leukemia (CML) patients, as they provide precious information for therapeutic decision-making. Molecular response levels are used to define whether a patient has an "optimal", "warning", or "failure" response to tyrosine kinase inhibitor (TKI) therapy. Mutation status may be useful to decide whether TKI therapy should be changed and which alternative TKI (or TKIs) are most likely to be effective. Real-time quantitative polymerase chain reaction (RQ-qPCR) and Sanger sequencing are currently the gold standard for molecular response monitoring and mutation testing, respectively. However, in recent years, novel technologies such as digital PCR (dPCR) and next-generation sequencing (NGS) have been evaluated. Here, we critically describe the main features of these old and novel technologies, provide an overview of the recently published studies assessing the potential clinical value of dPCR and NGS, and discuss how the state of the art might evolve in the next years.
journal_name
J Clin Medjournal_title
Journal of clinical medicineauthors
Soverini S,Bernardi S,Galimberti Sdoi
10.3390/jcm9123865subject
Has Abstractpub_date
2020-11-27 00:00:00issue
12issn
2077-0383pii
jcm9123865journal_volume
9pub_type
杂志文章,评审abstract：:In the last decade, it has been well-established that tumor-infiltrating myeloid cells fuel not only the process of carcinogenesis through cancer-related inflammation mechanisms, but also tumor progression, invasion, and metastasis. In particular, tumor-associated macrophages (TAMs) are the most abundant leucocyte sub...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm9103226
更新日期：2020-10-08 00:00:00
abstract：:This article presents the technical aspects of the Polish fetal cardiac interventions (FCI) program, including preparation of the team and modifications in the technique of the procedure that aim to increase its safety for the mother and the fetus. Over 9 years, 128 FCI in 113 fetuses have been performed: 94 balloon a...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9092888
更新日期：2020-09-07 00:00:00
abstract：:Amiodarone is recommended for shock-refractory ventricular arrhythmia during resuscitation; however, it is unknown whether amiodarone is effective for preventing ventricular arrhythmia recurrence in out-of-hospital cardiac arrest (OHCA) survivors treated with targeted temperature management (TTM). We investigated the ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8020244
更新日期：2019-02-13 00:00:00
abstract：:Air pollution is a major environmental risk to health and a global public health concern. In 2016, according to the World Health Organization (WHO), ambient air pollution in cities and rural areas was estimated to cause 4.2 million premature deaths. It is estimated that around 91% of the world's population lives in pl...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm10010077
更新日期：2020-12-28 00:00:00
abstract：:Intraovarian platelet-rich plasma (PRP) infusion was recently introduced in the context of addressing ovarian insufficiency. Reporting on its effectiveness prior to adopting in clinical routine practice is imperative. This study aims to provide pilot data regarding PRP application for ovarian rejuvenation. Four pilot ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9061809
更新日期：2020-06-10 00:00:00
abstract：:Restless legs syndrome (RLS) is highly prevalent among pregnant women. In the present study, a neurological-obstetrical sample of 561 postpartum women was retrospectively screened for RLS symptoms during pregnancy and in the first 12 weeks postpartum. The first screening took place within 1 to 6 days of delivery (T0) ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9093046
更新日期：2020-09-21 00:00:00
abstract：:The prognosis of multiple myeloma (MM) has improved with the introduction of novel agents. These data are largely derived from clinical trials and might not reflect real-world patient outcomes accurately. We surveyed real-world data from 284 patients newly diagnosed with MM between 2010 and 2018 in Miyazaki Prefecture...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm10010105
更新日期：2020-12-30 00:00:00
abstract：:Infections with SARS-CoV-2 can result in severe clinical manifestations. As such patients present with systemic inflammation, we studied the prevalence and predictive value of anemia of inflammation (AI) or functional iron deficiency (FID), originating from immune-mediated alterations of iron homeostasis. Within this ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9082429
更新日期：2020-07-29 00:00:00
abstract：:In patients with Cushing's disease (CD), prompt diagnosis and treatment are essential for favorable long-term outcomes, although this remains a challenging task. The differential diagnosis of CD is still difficult in some patients, even with an organized stepwise diagnostic approach. Moreover, despite the use of high-...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm8111951
更新日期：2019-11-12 00:00:00
abstract：:This study aimed to evaluate the effect of bladder neck preservation (BNP) on long-term urinary continence after robot-assisted laparoscopic prostatectomy (RALP). We systematically searched the PubMed, Embase, and Cochrane Library databases to identify studies that assessed the difference in urinary continence and onc...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm8122068
更新日期：2019-11-24 00:00:00
abstract：:Traumatic brain injury is not a discrete event but an unfolding sequence of damage to the central nervous system. Not only the acute phase but also the subacute and chronic period after injury, i.e., during inpatient rehabilitation, is characterized by multiple neurotransmitter alterations, cellular dysfunction, and m...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm4091815
更新日期：2015-09-22 00:00:00
abstract：:Background: Urinary calprotectin is a novel biomarker that distinguishes between intrinsic or prerenal acute kidney injury (AKI) in different studies. However, these studies were based on different populations and different AKI criteria. We evaluated the diagnostic accuracy of urinary calprotectin and compared its dia...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm8010074
更新日期：2019-01-10 00:00:00
abstract：:This study evaluated whether inter-hospital transfer (IHT) after the return of spontaneous circulation (ROSC) was associated with poor neurological outcomes after 6 months in post-cardiac-arrest patients treated with targeted temperature management (TTM). We used data from the Korean Hypothermia Network prospective re...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9061979
更新日期：2020-06-24 00:00:00
abstract：:Computer-aided diagnosis (CAD) and other risk stratification systems may improve ultrasound image interpretation. This prospective study aimed to compare the diagnostic performance of CAD and the European Thyroid Imaging Reporting and Data System (EU-TIRADS) classification applied by physicians with S-Detect 2 softwar...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9082495
更新日期：2020-08-03 00:00:00
abstract：:Low skeletal muscle mass (LSMM) is increasingly recognized for its predictive value for adverse events in cancer patients. In specific, the predictive value of LSMM has been demonstrated for anti-cancer drug toxicity in a variety of cancer types and anti-cancer drugs. However, due to the limited sample size and study ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9113780
更新日期：2020-11-23 00:00:00
abstract：:Overactivated osteoclasts lead to many bone diseases, including osteoporosis and rheumatoid arthritis. The p38 MAPK (p38) is an essential regulator of the receptor activator of nuclear factor-κB ligand (RANKL)-mediated osteoclastogenesis and bone loss. We previously reported TAT conjugated thioredoxin-interacting prot...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8040431
更新日期：2019-03-29 00:00:00
abstract：:The aim of our study was to evaluate redox status, enzymatic and non-enzymatic antioxidant barriers, oxidative damage of proteins, lipids and DNA, as well as concentration of coenzyme Q10 and vitamins A and E in patients with chronic granulomatous disease (CGD). The study was performed on fifteen Caucasian individuals...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9051397
更新日期：2020-05-09 00:00:00
abstract：:Anemia is a common feature of liver and bowel diseases. Although the main causes of anemia in these conditions are represented by gastrointestinal bleeding and iron deficiency, autoimmune hemolytic anemia should be considered in the differential diagnosis. Due to the epidemiological association, autoimmune hemolytic a...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm10030423
更新日期：2021-01-22 00:00:00
abstract：INTRODUCTION:Immunoglobulin G4-related disease (IgG4-RD) is a systemic immune-mediated disease characterised pathologically by the infiltration of IgG4-bearing plasma cells into the involved organs. Autoimmune pancreatitis (AIP) is a form of chronic pancreatitis with a heavy lymphocytic infiltration and two distinct hi...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9020409
更新日期：2020-02-03 00:00:00
abstract：:Fibrillary glomerulonephritis (FGN) is a rare glomerular disease characterized by glomerular deposition of randomly arranged non-amyloid fibrils. FGN has a poor renal prognosis and its optimal treatment is a medical challenge. Rituximab therapy has recently emerged as a promising approach even though its mechanism of ...
journal_title：Journal of clinical medicine
pub_type：
doi：10.3390/jcm7110430
更新日期：2018-11-09 00:00:00
abstract：:The relationship between bruxism and tooth wear is contentious in the literature. The pathophysiological processes of tooth wear may be complicated by the relationship between bruxism and gastroesophageal reflux disease (GERD). The objective of this study was to evaluate the associations among bruxism, GERD, and tooth...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm7110417
更新日期：2018-11-06 00:00:00
abstract：:The relationship between obesity and asthma exacerbations is still under debate. The aim of our work is to analyse the relationship between obesity and hospital re-admissions in asthmatics. A review was retrospectively performed on all hospital admissions of adult patients due to asthma exacerbation occurring in our h...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9010221
更新日期：2020-01-14 00:00:00
abstract：:Active vision therapy using perceptual learning and/or dichoptic or binocular environments has shown its potential effectiveness in amblyopia, but some doubts remain about the type of stimuli and the mode and sequence of presentation that should be used. A search was performed in PubMed, obtaining 143 articles with in...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm9123985
更新日期：2020-12-09 00:00:00
abstract：:Patients with chronic obstructive pulmonary disease (COPD), asthma and interstitial lung diseases (ILD) frequently suffer from cardiovascular comorbidities (CVC). Exercise training is a cornerstone intervention for the management of these conditions, however recommendations on tailoring programmes to patients sufferin...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm8091458
更新日期：2019-09-13 00:00:00
abstract：BACKGROUND:In mildly to moderately malnourished adolescent patients with anorexia nervosa (AN), accelerated refeeding protocols using higher initial calory supply coupled with phosphate supplements were not associated with a higher incidence of refeeding syndrome (RS). It is unclear whether this is also a feasible appr...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9051535
更新日期：2020-05-19 00:00:00
abstract：:Community-acquired pneumonia (CAP) remains the first cause of morbidity and mortality worldwide due to infection. Several aspects such as severity and host response are related to its clinical course and outcome. Beyond the acute implications that the infection provokes in the host, pneumonia also has long-term negati...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm9020549
更新日期：2020-02-18 00:00:00
abstract：:Osteogenesis imperfecta (OI) is a connective tissue disorder that is characterized by low bone density leading to recurrent fractures. The efficacy of the anti-resorption drug denosumab for OI with osteoporosis is still largely unknown. We herein describe the clinical outcomes of eight osteoporotic cases of OI to exam...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm7120479
更新日期：2018-11-24 00:00:00
abstract：:Creatinine is produced in muscle metabolism as the end-product of creatine phosphate and is subsequently excreted principally by way of the kidneys, predominantly by glomerular filtration. Blood creatinine assays constitute the most common clinically relevant measure of renal function. The use of individual patient-le...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm4071348
更新日期：2015-06-26 00:00:00
abstract：BACKGROUND:Heart rate variability (HRV) as an accurate, noninvasive measure of the Autonomous Nervous System (ANS) can reflect mental health (e.g., stress, depression, or anxiety). Tai Chi and Yoga (Tai Chi/Yoga), as the most widely practiced mind⁻body exercises, have shown positive outcomes of mental health. To date, ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm7110404
更新日期：2018-10-31 00:00:00
abstract：:Stress response to robot-assisted colorectal surgery is largely unknown. Therefore, we conducted a prospective comparative nonrandomized study evaluating the perioperative dynamics of chemokines: IL-8/CXCL8, MCP-1/CCL2, MIP-1α/CCL3, MIP-1β/CCL4, RANTES/CCL5, and eotaxin-1/CCL11 in 61 colorectal cancer patients followi...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8060879
更新日期：2019-06-19 00:00:00
