Different transcriptome profiles between human retinoblastoma Y79 cells and an etoposide-resistant subline reveal a chemoresistance mechanism.

Abstract:

BACKGROUND:Retinoblastoma (RB) is the most frequent pediatric retinal tumor. In the present study, to elucidate chemoresistance mechanisms and identify potential biomarkers in RB, we utilized RNA sequencing (RNAseq) technological platforms to reveal transcriptome profiles and identify any differentially expressed genes (DEGs) between an etoposide drug-resistant subline (Y79/EDR) and parental Y79 cells. METHODS:To test whether Y79/EDR cells showed resistance to antineoplastic agents for RB, we treated the cells with etoposide, carboplatin and vincristine and analyzed them with a Cell Counting Kit-8 (CCK-8). Y79/EDR and parental Y79 cells were used for RNAseq and bioinformatics analysis to enable a genome-wide review of DEGs between the two lines using the DESeq R package (1.10.1). Then, DEG enrichment in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways was analyzed with KOBAS software. Next, real-time quantitative reverse transcription polymerase chain reaction (real time QRT-PCR) and cytotoxicity assays were performed to experimentally and functionally validate the identified candidate biomarkers. RESULTS:Y79/EDR cells showed resistance to etoposide, carboplatin and vincristine at different concentrations. In total, 524 transcripts were differentially expressed in Y79/EDR cells based on analysis of fragments per kilobase of transcript per million fragments mapped (FPKM); among these, 57 genes were downregulated and 467 genes were upregulated in Y79/EDR cells compared to parental Y79 cells. We selected candidate DEGs, including ARHGAP9, HIST1H4H, RELN, DDIT4, HK2, STC1 and PFKFB4, for mRNA expression validation with real time QRT-PCR assays and found that the expression levels determined by real time QRT-PCR were consistent with the RNAseq data. Further studies involving downregulation of ARHGAP9 with a specific siRNA showed that ARHGAP9 altered the cellular sensitivity of Y79 cells to etoposide and carboplatin. CONCLUSION:Our initial findings provided a genomic view of the transcription profiles of etoposide-induced acquired resistance in RB. Follow-up studies indicated that ARHGAP9 might be a chemoresistance biomarker in RB, providing insight into potential therapeutic targets for overcoming acquired chemoresistance in RB. These findings can aid in understanding and overcoming chemoresistance during treatment of RB in the clinic.

journal_name

BMC Ophthalmol

journal_title

BMC ophthalmology

authors

Song WP,Zheng S,Yao HJ,Zhou XF,Li R,Zhang CY,Zhao JY,Wang LW,Shao RG,Li L

doi

10.1186/s12886-020-01348-6

subject

Has Abstract

pub_date

2020-03-06 00:00:00

pages

92

issue

1

issn

1471-2415

pii

10.1186/s12886-020-01348-6

journal_volume

20

pub_type

杂志文章
  • Congenital aniridia with cataract: case series.

    abstract:BACKGROUND:This study evaluates patients with congenital aniridia and cataract who underwent phacoemulsification, capsular tension ring placement, and foldable intraocular lens implantation. METHODS:In this prospective case series, 10 patients (17 eyes) underwent cataract surgery via a 3.2 mm clear corneal incision. A...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-017-0503-6

    authors: Wang JD,Zhang JS,Xiong Y,Li J,Li XX,Liu X,Zhao J,Tsai FF,Vishal J,You QS,Huang Y,Wan XH

    更新日期:2017-07-04 00:00:00

  • Successful treatment of endogenous endophthalmitis with extensive subretinal abscess: a case report.

    abstract:BACKGROUND:Endogenous endophthalmitis could lead to a devastating outcome without a prompt and appropriate management. We report a case of advanced endogenous Klebsiella pneumoniae endophthalmitis with extensive subretinal abscess that was successfully treated with a vitrectomy. CASE PRESENTATION:A systemically well 6...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-018-0908-x

    authors: Xu H,Fu B,Lu C,Xu L,Sun J

    更新日期:2018-09-05 00:00:00

  • Intravitreal dexamethasone implant Ozurdex® in naïve and refractory patients with different subtypes of diabetic macular edema.

    abstract:BACKGROUND:Diabetic macular edema (DME) can be treated with different alternatives, among them Dexamethasone intravitreal implant 0.7 mg (DEX 0.7) has demonstrated that may improve both central macular thickness (CMT) and best corrected visual acuity (BCVA). This study aimed to evaluate the effect of the intravitreal d...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-018-1022-9

    authors: Castro-Navarro V,Cervera-Taulet E,Navarro-Palop C,Monferrer-Adsuara C,Hernández-Bel L,Montero-Hernández J

    更新日期:2019-01-11 00:00:00

  • Ocular abnormalities in a large patient cohort with retinitis pigmentosa in Western China.

    abstract:BACKGROUND:To report the prevalence of ocular abnormalities and investigate visual acuity in a large cohort of retinitis pigmentosa (RP) patients in Western China. METHODS:The medical records and ophthalmic examination reports of 2127 eyes of 1065 RP patients at one eye hospital were retrospectively reviewed to determ...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-020-01797-z

    authors: Tan L,Long Y,Li Z,Ying X,Ren J,Sun C,Meng X,Li S

    更新日期:2021-01-18 00:00:00

  • First identification of Gordonia sputi in a post-traumatic endophthalmitis patient - a case report and literatures review.

    abstract:BACKGROUND:We present a case of post-traumatic endophthalmitis with relatively good prognosis caused by Gordonia sputi, which, to our knowledge is the first case in the literature. CASE PRESENTATION:A 24 year old man, who underwent an intraocular foreign body extraction half a month before presentation in the left eye...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-017-0573-5

    authors: Fang W,Li J,Cui HS,Jin X,Zhai J,Dai Y,Li Y

    更新日期:2017-10-11 00:00:00

  • Purtscher-like retinopathy associated with dermatomyositis.

    abstract:BACKGROUND:To report a rare case of bilateral Purtscher-like retinopathy in a Chinese female patient with dermatomyositis. CASE PRESENTATION:An 18-year-old woman with lower extremity muscle weakness was admitted due to decreased vision of both eyes for two weeks. Ophthalmic examination revealed violaceous eruption on ...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/1471-2415-13-36

    authors: Yan Y,Shen X

    更新日期:2013-07-24 00:00:00

  • The effect of phacoemulsification plus goniosynechialysis in acute and chronic angle closure patients with extensive goniosynechiae.

    abstract:BACKGROUND:Patients with primary angle closure/glaucoma (PAC/PACG) with extensive peripheral anterior synechiae (PAS), and coexisting cataract, increasingly have been treated with phacoemulsification combined with goniosynechialysis (Phaco-GSL). Since the mechanisms of acute and chronic PAC/PACG may differ, the treatme...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-019-1070-9

    authors: Tian T,Li M,Pan Y,Cai Y,Fang Y

    更新日期:2019-03-04 00:00:00

  • Characterization of ex vivo cultured neuronal- and glial- like cells from human idiopathic epiretinal membranes.

    abstract:BACKGROUND:Characterization of the neuro-glial profile of cells growing out of human idiopathic epiretinal membranes (iERMs) and testing their proliferative and pluripotent properties ex vivo is needed to better understand the pathogenesis of their formation. METHODS:iERMs obtained during uneventful vitrectomies were ...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/1471-2415-14-165

    authors: Andjelić S,Lumi X,Yan X,Graw J,Moe MC,Facskó A,Hawlina M,Petrovski G

    更新日期:2014-12-23 00:00:00

  • The impact of irregular corneal shape parameters on visual acuity and contrast sensitivity.

    abstract:BACKGROUND:To understand which irregular corneal parameters determine the visual quality in keratoconus subjects. METHODS:The cross-sectional study examined the eyes of 44 subjects, graded from the first to third keratoconus stages by Amsler-Krumeich classification. We obtained measurements in two ways: (a) by project...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-020-01737-x

    authors: Liduma S,Luguzis A,Krumina G

    更新日期:2020-11-26 00:00:00

  • Safety and efficacy of a standardized intracameral combination of mydriatics and anesthetic for cataract surgery in type-2 diabetic patients.

    abstract:BACKGROUND:Cataract surgery in diabetics is more technically challenging due to a number of factors including poor intraoperative pupil dilation and a higher risk of vision threatening complications. This study evaluates the safety and efficacy of an intracameral combination of 2 mydriatics and 1 anesthetic (ICMA, Mydr...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1186/s12886-020-01343-x

    authors: Labetoulle M,Behndig A,Tassignon MJ,Nuijts R,Mencucci R,Güell JL,Pleyer U,Szaflik J,Rosen P,Bérard A,Chiambaretta F,Cochener-Lamard B,Intracameral Mydrane (ICMA), Ethics Group.

    更新日期:2020-03-03 00:00:00

  • The mutation spectrum in familial versus sporadic congenital cataract based on next-generation sequencing.

    abstract:BACKGROUND:Congenital cataract (CC) is a significant cause of lifelong visual loss, and its genetic diagnosis is challenging due to marked genetic heterogeneity. The purpose of this article is to report the genetic findings in sporadic and familial CC patients. METHODS:Patients (n = 53) who were clinically diagnosed w...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-020-01567-x

    authors: Fan F,Luo Y,Wu J,Gao C,Liu X,Mei H,Zhou X

    更新日期:2020-09-03 00:00:00

  • The association between intraocular pressure and different combination of metabolic syndrome components.

    abstract:BACKGROUND:Although the association between metabolic syndrome and intraocular pressure is well known, the relationship between the intraocular pressure and different combination of the components of metabolic syndrome has not been actively researched yet. The study aimed to investigate the relationship between the int...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-016-0263-8

    authors: Son J,Koh H,Son J

    更新日期:2016-06-06 00:00:00

  • Ocular dimensions of the Chinese adolescents with keratoconus.

    abstract:BACKGROUND:Adolescent KC is a special segment of the general KC population because an adolescents's eyes are still susceptible to blurred vision and optical defocus during the sensitive period of ocular and visual development. In the present study, we evaluated the ocular dimensions of 53 KC adolescents. METHODS:One h...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-018-0713-6

    authors: Jian W,Shen Y,Chen Y,Tian M,Zhou X

    更新日期:2018-02-13 00:00:00

  • Protection of retinal function and morphology in MNU-induced retinitis pigmentosa rats by ALDH2: an in-vivo study.

    abstract:BACKGROUND:Retinitis pigmentosa (RP) is a kind of inherited retinal degenerative diseases characterized by the progressive loss of photoreceptors. RP has been a conundrum without satisfactory countermeasures in clinic until now. Acetaldehyde dehydrogenase 2 (ALDH2), a major enzyme involved in aldehyde detoxification, h...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-020-1330-8

    authors: Yan W,Long P,Wei D,Yan W,Zheng X,Chen G,Wang J,Zhang Z,Chen T,Chen M

    更新日期:2020-02-18 00:00:00

  • A comparative study of glaucoma referrals in Southeast Scotland: effect of the new general ophthalmic service contract, Eyecare integration pilot programme and NICE guidelines.

    abstract:BACKGROUND:Glaucoma is a progressive disease responsible for the second commonest cause of blindness in the UK. Identifying appropriate patients for hospital care remains an ongoing challenge for all UK hospital glaucoma services. The purpose of our study is to evaluate accuracy and outcome of community optometry refer...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-015-0161-5

    authors: El-Assal K,Foulds J,Dobson S,Sanders R

    更新日期:2015-12-07 00:00:00

  • Conjunctival lymphoma during pregnancy: a case report.

    abstract:BACKGROUND:To present a case of conjunctival lymphoma in a young woman complicated by pregnancy. CASE PRESENTATION:A 38-year-old previously healthy woman presented with a 2-year history of progressive right blepharoptosis. Giant papillomatous sessile masses were identified in the upper and lower fornix bilaterally and...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-017-0518-z

    authors: Ong SJ,Jung SM,Lin HC

    更新日期:2017-07-27 00:00:00

  • A 8-year retrospective clinical analysis of 272 patients of epidemic Keratoconjunctivitis in Beijing, China.

    abstract:BACKGROUND:Epidemic keratoconjunctivitis (EKC) is one of the most common eye infections worldwide. The analysis of clinical manifestations in different age groups help better know the disease. This study aims to provide more detailed analysis of 272 cases of EKC in 8 years, describe the differences of the clinical feat...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-019-1266-z

    authors: Hou W,Sun X,Feng J,Zhang Y,Wang Z

    更新日期:2019-12-19 00:00:00

  • Extensive bilateral corneal edema 6 weeks after cataract surgery: Keratopathy due to Asclepias physocarpa: a case report.

    abstract:BACKGROUND:Surgeons may be unaware of the ability of plant toxins to cause corneal damage. Therefore, corneal damage following intraocular surgery due to plant toxins may be misdiagnosed as postoperative infection. CASE PRESENTATION:A 74-year-old man presented with hyperemia and reduced visual acuity in both eyes 6 we...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-017-0400-z

    authors: Matsuura K,Hatta S,Terasaka Y,Inoue Y

    更新日期:2017-01-18 00:00:00

  • Heimann-Bielschowsky phenomenon and hypotropic DVD in case of monocular vision loss: a case report.

    abstract:BACKGROUND:The Heimann-Bielschowsky phenomenon (HBP) is an underrecognized condition characterized by slow, pendular, vertical oscillations of the eye accompanying monocular vision loss. Hypotropic dissociated vertical deviation (DVD) is another rare condition induced by asymmetric visual input. This report documents a...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-020-01502-0

    authors: Choi HJ,Chun BY

    更新日期:2020-06-26 00:00:00

  • Single-session photodynamic therapy combined with intravitreal bevacizumab and triamcinolone for neovascular age-related macular degeneration.

    abstract:BACKGROUND:To evaluate the efficacy of triple therapy consisting of single-session photodynamic therapy (PDT), intravitreal bevacizumab (IVB) and intravitreal triamcinolone (IVT) as initial pulse therapy followed by repeat intravitreal bevacizumab injections for maintenance treatment in neovascular age-related macular ...

    journal_title:BMC ophthalmology

    pub_type: 临床试验,杂志文章

    doi:10.1186/1471-2415-7-10

    authors: Ahmadieh H,Taei R,Soheilian M,Riazi-Esfahani M,Karkhaneh R,Lashay A,Azarmina M,Dehghan MH,Moradian S

    更新日期:2007-06-07 00:00:00

  • Comparison of the biometric values obtained by two different A-mode ultrasound devices (Eye Cubed vs. PalmScan): a transversal, descriptive, and comparative study.

    abstract:BACKGROUND:To assess the reliability of the measurements obtained with the PalmScan, when compared with another standardized A-mode ultrasound device, and assess the consistency and correlation between the two methods. METHODS:Transversal, descriptive, and comparative study. We recorded the axial length (AL), anterior...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/1471-2415-10-8

    authors: Velez-Montoya R,Shusterman EM,López-Miranda MJ,Mayorquin-Ruiz M,Salcedo-Villanueva G,Quiroz-Mercado H,Morales-Cantón V

    更新日期:2010-03-24 00:00:00

  • Assessment of diabetic retinopathy using two ultra-wide-field fundus imaging systems, the Clarus® and Optos™ systems.

    abstract:BACKGROUND:The ability to image wide fundus fields and to conduct swift, non-invasive examinations is increasingly important with the escalation in patients with diabetic retinopathy (DR). METHODS:Fifty eyes of 28 consecutive patients with DR were examined in this prospective observational study. A total of 46 eyes, 2...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-018-1011-z

    authors: Hirano T,Imai A,Kasamatsu H,Kakihara S,Toriyama Y,Murata T

    更新日期:2018-12-20 00:00:00

  • Open globe and penetrating eyelid injuries from fish hooks.

    abstract:BACKGROUND:A few case reports have described accidental eye injuries caused by fish hooks. The severity of ocular injuries is dependent on the involved ocular structures. Severe ocular injuries due to fish hooks are rare. We describe open globe and penetrating eyelid injuries from fish hooks at the Baltic Sea. METHODS...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-019-1040-2

    authors: Purtskhvanidze K,Saeger M,Treumer F,Nölle B,Roider J

    更新日期:2019-01-21 00:00:00

  • Retinal artery occlusion as the manifestation of left atrial myxoma: a case report.

    abstract:BACKGROUND:Retinal artery occlusion caused by myxoma is relatively rare. There are several points that should be taken into consideration to avoid overlooking this disorder. CASE PRESENTATION:This case report describes a 43-year-old woman with sudden vision loss in her left eye for 20 days after single sudden syncope....

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/1471-2415-14-164

    authors: Yu Y,Zhu Y,Dong A,Su Z

    更新日期:2014-12-23 00:00:00

  • The effects of oral and topical corticosteroid in rabbit corneas.

    abstract:BACKGROUND:To determine the most effective route of administration of corticosteroids in the treatment of ocular surface disease, by characterizing the difference between oral prednisolone and topical dexamethasone administration using an animal model. METHODS:Pharmacokinetic analyses determined the corticosteroid con...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-016-0339-5

    authors: Araki-Sasaki K,Katsuta O,Mano H,Nagano T,Nakamura M

    更新日期:2016-09-05 00:00:00

  • Area of the cone interdigitation zone in healthy Chinese adults and its correlation with macular volume.

    abstract:BACKGROUND:Numerous studies have suggested that the integrity of the cone interdigitation zone (IZ) could be considered to be a marker of photoreceptor damage and its recovery. However, little is known about the IZ in healthy eyes. Our present study was to measure the cone IZ area by optical coherence tomography (OCT),...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-018-0862-7

    authors: Gu R,Deng G,Jiang Y,Jiang C,Xu G

    更新日期:2018-08-01 00:00:00

  • The association between socioeconomic status and visual impairments among primary glaucoma: the results from Nationwide Korean National Health Insurance Cohort from 2004 to 2013.

    abstract:BACKGROUND:Glaucoma is one of the most leading causes of permanent visual impairments in Korea, and social expenses spent for the glaucoma are increasing. This study is to identify association between socioeconomic status and the visual impairments caused by primary glaucoma in Korea. METHODS:This study is based on a ...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-017-0551-y

    authors: Sung H,Shin HH,Baek Y,Kim GA,Koh JS,Park EC,Shin J

    更新日期:2017-08-23 00:00:00

  • The efficacy and safety of Retcam in detecting neonatal retinal hemorrhages.

    abstract:BACKGROUND:To investigate the ability of characterizing neonatal retinal hemorrhage (RH) using RetCam in healthy newborns and the systemic effects during the procedure. METHODS:This prospective study enrolled 68 healthy newborns aged 2 to 4 days old. The RH was imaged and classified according to the location and numbe...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-018-0887-y

    authors: Chen F,Cheng D,Pan J,Huang C,Cai X,Tian Z,Lu F,Shen L

    更新日期:2018-08-20 00:00:00

  • Evaluation of changes in choroidal thickness after implantable collamer lens surgery in high myopia patients with graves' Ophthalmopathy (inactive phase).

    abstract:BACKGROUND:To evaluate the safety and effectiveness of the Visian Implantable Collamer Lens (ICL) implantation in high myopic patients with inactive Graves' ophthalmopathy (GO) by observing the changes of choroidal thickness (CT). METHODS:Eight patients (16 eyes) with high myopia accompanied with inactive GO were sele...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章

    doi:10.1186/s12886-020-01612-9

    authors: He F,Liu Y,Jia R,Zhang J

    更新日期:2020-08-25 00:00:00

  • Readability of patient education materials in ophthalmology: a single-institution study and systematic review.

    abstract:BACKGROUND:Patient education materials should be written at a level that is understandable for patients with low health literacy. The aims of this study are (1) to review the literature on readability of ophthalmic patient education materials and (2) to evaluate and revise our institution's patient education materials ...

    journal_title:BMC ophthalmology

    pub_type: 杂志文章,评审

    doi:10.1186/s12886-016-0315-0

    authors: Williams AM,Muir KW,Rosdahl JA

    更新日期:2016-08-03 00:00:00