Abstract:
:Topical application of vascular endothelial growth factor A (VEGFA) inhibitors including Bevacizumab is used for antiangiogenic therapy at the ocular surface. While clinical studies have suggested that this approach is well-tolerated, the effect of the drug on limbal epithelial stem cells has not been studied. In this study, the effect of Bevacizumab on phenotype and functionality of putative limbal epithelial stem cells (SC) was investigated. The effect of Bevacizumab on human limbal epithelial cells was assessed in terms of metabolic activity and scratch wound closure. The different treatment groups featured no difference in proliferation and colony forming efficiency (CFE) of limbal epithelial cells or their putative SC marker expression. A significant delay in scratch closure of all the Bevacizumab-treated groups was detected at 4 h. RNA and protein quantification indicated a dose-responsive increase of keratin 3. VEGFA RNA expression also increased while VEGFC and D as well as VEGFR1, 2 and 3 were unchanged. This study highlights previously unknown effects of Bevacizumab on cultured putative limbal epithelial SC: a dose-related increase of keratin 3, an increase in VEGFA as well as a delay in scratch wound closure. These in vitro data should be considered when using Bevacizumab in the context of limbal epithelial SC transplantation.
journal_name
J Clin Medjournal_title
Journal of clinical medicineauthors
Notara M,Lentzsch A,Clahsen T,Behboudifard S,Braun G,Cursiefen Cdoi
10.3390/jcm8111925subject
Has Abstractpub_date
2019-11-09 00:00:00issue
11issn
2077-0383pii
jcm8111925journal_volume
8pub_type
杂志文章abstract::Early diagnosis and timely management of tooth or dental material wear is imperative to avoid extensive restorations. Previous studies suggested different methods for tooth wear assessment, but no study has developed a three-dimensional (3D) superimposition technique applicable in cases where tooth surfaces, other tha...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9123937
更新日期:2020-12-04 00:00:00
abstract::An increasing number of patients with coronary artery disease are at high operative risk due to advanced age, severe comorbidities, complex coronary anatomy, and reduced ejection fraction. Consequently, these high-risk patients are often offered percutaneous coronary intervention (PCI) as an alternative to coronary ar...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9113414
更新日期:2020-10-25 00:00:00
abstract::Abnormalities in body composition are associated with poor prognosis in cancer patients. We investigated the association between body composition and 90-day mortality in cancer patients who developed septic shock. We included consecutive septic shock patients with active cancer from 2010 to 2017. The muscle area at th...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm8101583
更新日期:2019-10-02 00:00:00
abstract::The outcome of patients with hepatocellular carcinoma (HCC) is still poor. Decorin is a small leucine-rich proteoglycan, which exerts antiproliferative and antiangiogenic properties in vitro. We aimed to investigate the associations of decorin with physical function and prognosis in patients with HCC. We enrolled 65 p...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9040936
更新日期:2020-03-28 00:00:00
abstract::Due to the widespread use of ultrasound, small thyroid nodules (TNs) ≤ 10 mm are common findings. Standardized approaches for the risk stratification of TNs with Thyroid Imaging Reporting and Data Systems (TIRADS) were evaluated for the clinical routine. With TIRADS, the risk of malignancy in TNs is calculated by scor...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9010236
更新日期:2020-01-16 00:00:00
abstract::Hereditary hemorrhagic telangiectasia (HHT) is characterized by mucocutaneous telangiectases and visceral vascular malformations. Individuals suffering from HHT have a significantly increased risk of bacterial infections, but the mechanisms involved in this are not clear. White blood cell subpopulations were estimated...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9030767
更新日期:2020-03-12 00:00:00
abstract::Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is an ultra-rare autosomal recessive disorder of nucleoside metabolism that is caused by mutations in the nuclear thymidine phosphorylase gene (TYMP) gene, encoding for the enzyme thymidine phosphorylase. There are currently no approved treatments for MNGIE...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm8040457
更新日期:2019-04-05 00:00:00
abstract::Cutaneous nerve entrapment plays an important role in neuropathic pain syndrome. Due to the advancement of ultrasound technology, the cutaneous nerves can be visualized by high-resolution ultrasound. As the cutaneous nerves course superficially in the subcutaneous layer, they are vulnerable to entrapment or collateral...
journal_title:Journal of clinical medicine
pub_type: 杂志文章,评审
doi:10.3390/jcm7110457
更新日期:2018-11-21 00:00:00
abstract::The geographic spread of 2019 novel coronavirus (COVID-19) infections from the epicenter of Wuhan, China, has provided an opportunity to study the natural history of the recently emerged virus. Using publicly available event-date data from the ongoing epidemic, the present study investigated the incubation period and ...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9020538
更新日期:2020-02-17 00:00:00
abstract::Liquid fibrinogen is an injectable platelet concentrate rich in platelets, leukocytes, and fibrinogen obtained by blood centrifugation. The aim of this study was to analyze the release of different growth factors in the liquid fibrinogen at different times and to assess possible correlations between growth factors and...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9041099
更新日期:2020-04-12 00:00:00
abstract::Hepatitis C virus (HCV) infection develops into chronic hepatitis in over two-thirds of acute infections. While current treatments with direct-acting antivirals (DAAs) achieve HCV eradication in >95% of cases, no vaccine is available and re-infection can readily occur. Natural killer (NK) cells represent a key cellula...
journal_title:Journal of clinical medicine
pub_type: 杂志文章,评审
doi:10.3390/jcm9041030
更新日期:2020-04-06 00:00:00
abstract::Androgen/androgen receptor (AR) signaling is a significant driver of prostate cancer progression, therefore androgen-deprivation therapy (ADT) is often used as a standard form of treatment for advanced and metastatic prostate cancer patients. However, after several years of ADT, prostate cancer progresses to castratio...
journal_title:Journal of clinical medicine
pub_type: 杂志文章,评审
doi:10.3390/jcm8030354
更新日期:2019-03-13 00:00:00
abstract::This study aimed to investigate the periodontal health of patients with established rheumatoid arthritis (RA) in relation to oral microbiota, systemic and oral inflammatory mediators, and RA disease activity. Forty patients underwent full-mouth dental/periodontal and rheumatological examination, including collection o...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm8050630
更新日期:2019-05-08 00:00:00
abstract:BACKGROUND:Although the loop electrosurgical excision procedure (LEEP) is a brief procedure, it can cause severe pain and discomfort to patients in the absence of adequate sedation. An admixture of ketamine with propofol (ketofol), may reduce patient movement due to insufficient sedation while providing hemodynamic and...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm8070943
更新日期:2019-06-28 00:00:00
abstract:BACKGROUND AND PURPOSE:The aim of the study was to determine the effects of post-traumatically released High Mobility Group Box-1 protein (HMGB1) and extracellular histones on cardiomyocytes (CM). We also evaluated a therapeutic option to capture circulating histones after trauma, using a hemadsorption filter to treat ...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9051421
更新日期:2020-05-11 00:00:00
abstract:OBJECTIVE:In order to promote physical activity (PA) in patients with complicated type 2 diabetes, a better understanding of daily movement is required. We (1) objectively assessed PA in patients with type 2 diabetes, and (2) studied the association between muscle mass, dietary protein intake, and PA. Methods: We perfo...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9103104
更新日期:2020-09-25 00:00:00
abstract::This study evaluated the efficacy of Lactobacillus acidophilus YT1 (MENOLACTO) for alleviating menopausal symptoms. This study was a multi-center, randomized, double-blinded, placebo-controlled clinical trial involving female subjects (ages: 40-60 years) with menopausal symptoms and a Kupperman index (KMI) score ≥ 20....
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9072173
更新日期:2020-07-09 00:00:00
abstract::Coronary artery calcification (CAC) is a feature of coronary atherosclerosis and a well-known risk factor for cardiovascular disease (CVD). As the absence of CAC is associated with a lower incidence rate of CVD, measurement of a CAC score is helpful for risk stratification when the risk decision is uncertain. This was...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm10030457
更新日期:2021-01-25 00:00:00
abstract::The widely varying therapeutic response of patients with inflammatory bowel disease (IBD) continues to raise questions regarding the unclarified heterogeneity of pathological mechanisms promoting disease progression. While biomarkers for the differentiation of Crohn's disease (CD) versus ulcerative colitis (UC) have b...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9051360
更新日期:2020-05-06 00:00:00
abstract::Social media enables the public sharing of information. With the recent emphasis on transparency and the open sharing of information between doctors and patients, the intersection of social media and healthcare is of particular interest. Twitter is currently the most popular form of social media used for healthcare co...
journal_title:Journal of clinical medicine
pub_type: 杂志文章,评审
doi:10.3390/jcm7060121
更新日期:2018-05-28 00:00:00
abstract::The main risk factor for primary open-angle glaucoma (POAG) is increased intraocular pressure (IOP). It is of interest that about half of the patients have an IOP within the normal range (normal-tension glaucoma, NTG). Additionally, there is a group of patients with a high IOP but no glaucomatous neurodegeneration (oc...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9092979
更新日期:2020-09-15 00:00:00
abstract::We sought to clarify the correlation between non-protein respiratory quotient (npRQ) in indirect calorimetry and serum zinc (Zn) level in chronic liver diseases (CLDs, n = 586, 309 liver cirrhosis (LC) patients, median age = 63 years). Clinical parameters potentially linked to npRQ <0.85 (best cutoff point for the pro...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9010255
更新日期:2020-01-17 00:00:00
abstract::The repair of bone defects caused by trauma, infection or tumor resection is a major clinical orthopedic challenge. The application of bone grafts in orthopedic procedures is associated with a problem of inadequate vascularization in the initial phase after implantation. Meanwhile, the survival of cells within the imp...
journal_title:Journal of clinical medicine
pub_type: 杂志文章,评审
doi:10.3390/jcm8101602
更新日期:2019-10-03 00:00:00
abstract::Despite the increasing incidence of gastroesophageal junction adenocarcinoma (GEJA), the optimal treatment strategy for the disease remains unknown. The objective of this study was to describe treatment patterns for GEJA in the United States. The National Cancer Database was searched to identify all patients who under...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9113495
更新日期:2020-10-29 00:00:00
abstract:BACKGROUND:Our study aims to perform a meta-analysis of benefits and risks associated with the use of non-vitamin K oral anticoagulants (NOAC) versus vitamin K antagonists (VKA) in patients with a percutaneous coronary intervention (PCI) with a particular focus on the combination type: dual vs. dual antithrombotic ther...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9041120
更新日期:2020-04-14 00:00:00
abstract::Restless legs syndrome (RLS) is highly prevalent among pregnant women. In the present study, a neurological-obstetrical sample of 561 postpartum women was retrospectively screened for RLS symptoms during pregnancy and in the first 12 weeks postpartum. The first screening took place within 1 to 6 days of delivery (T0) ...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9093046
更新日期:2020-09-21 00:00:00
abstract::The limited number of available studies that examined the pathology of alcohol hangover focused on biomarkers of alcohol metabolism, oxidative stress and the inflammatory response to alcohol as potentially important determinants of hangover severity. The available literature on alcohol metabolism and oxidative stress ...
journal_title:Journal of clinical medicine
pub_type: 杂志文章,评审
doi:10.3390/jcm9113421
更新日期:2020-10-25 00:00:00
abstract::The Scapula Reposition Test (SRT) is proposed to determine if a relationship exists between scapular dyskinesis and shoulder pathology. The purpose of this study was to evaluate intra-rater and inter-rater reliability of the SRT in subjects with rotator cuff tendinopathy and scapular dyskinesis. In addition, we compar...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9010080
更新日期:2019-12-28 00:00:00
abstract:OBJECTIVE:Clinical presentation of patients with mitral paravalvular leakage (PVL) varies from asymptomatic to heart failure related with hemolytic anemia or pulmonary hypertension. We aimed to investigate the structural and functional characteristics of mitral PVL by multimodal imaging and their association with the s...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm10020222
更新日期:2021-01-10 00:00:00
abstract::C-reactive protein (CRP) and myxovirus resistance protein A (MxA) are associated with bacterial and viral infections, respectively. We conducted a prospective, multicenter, cross-sectional study of adults and children with febrile upper respiratory tract infections (URIs) to evaluate the diagnostic accuracy of a rapid...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm6100094
更新日期:2017-10-07 00:00:00