Abstract：
:Conventional 3D multicellular tumor spheroids of head and neck squamous cell carcinoma (HNSCC) consisting exclusively of cancer cells have some limitations. They are compact cell aggregates that do not interact with their extracellular milieu, thus suffering from both insufficient extracellular matrix (ECM) deposition and absence of different types of stromal cells. In order to better mimic in vivo HNSCC tumor microenvironment, we have constructed a 3D stroma-rich in vitro model of HNSCC, using cancer-associated MeWo skin fibroblasts and FaDu pharynx squamous cell carcinoma. The expression of stromal components in heterospheroids was confirmed by immunochemical staining. The generated co-culture FaDu/MeWo spheroids were applied to study penetration, distribution and antitumor efficacy of photoactive drugs such as Temoporfin and Chlorin e6 used in the photodynamic therapy flow cytometry and fluorescence microscopy techniques. We also investigated the distribution of photodiagnostic agent Indocyanine Green. We demonstrated that the presence of stroma influences the behavior of photoactive drugs in different ways: (i) No effect on Indocyanine Green distribution; (ii) lower accumulation of Chlorin e6; (iii) better penetration and PDT efficiency of Temoporfin. Overall, the developed stroma-rich spheroids enlarge the arsenal of in vitro pre-clinical models for high-throughput screening of anti-cancer drugs.
journal_name
J Clin Medjournal_title
Journal of clinical medicineauthors
Yakavets I,Jenard S,Francois A,Maklygina Y,Loschenov V,Lassalle HP,Dolivet G,Bezdetnaya Ldoi
10.3390/jcm8101686subject
Has Abstractpub_date
2019-10-15 00:00:00issue
10issn
2077-0383pii
jcm8101686journal_volume
8pub_type
杂志文章abstract：BACKGROUND:Population aging poses unprecedented demands on the healthcare system. There is also a scarcity of evidence on self-care intervention to improve objective measures of morbidity and aging-associated functional and physiological measures in a low-income multi-ethnic population setting. METHODS:We conducted a ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9030885
更新日期：2020-03-24 00:00:00
abstract：:Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder, with the characteristic symptoms of chronic abdominal pain and altered bowel habits (diarrhea, constipation, or both). IBS is a highly prevalent condition, which negatively affects quality of life and is a significant burden on global healthcare...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm6110104
更新日期：2017-11-03 00:00:00
abstract：:The aim of the study was to evaluate the effect of titanium bone fixations on mitochondrial activity, reactive oxygen species (ROS) production, glutathione metabolism, and selected markers of oxidative/nitrosative stress in the periosteum-like tissue of patients treated with mandible fractures. The study group consist...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8010127
更新日期：2019-01-21 00:00:00
abstract：:Polycystic ovary syndrome (PCOS) is a common condition, affecting 5⁻10% of women of reproductive age worldwide. It has serious reproductive implications and causes mood disorders and metabolic disorders, such as type-2 diabetes. Because PCOS reflects multiple abnormalities, there is no single drug that can treat all i...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm7070179
更新日期：2018-07-22 00:00:00
abstract：:Oxidative stress arises when cellular antioxidant defences become overwhelmed by a surplus generation of reactive oxygen species (ROS). Once this occurs, many cellular biomolecules such as DNA, lipids, and proteins become susceptible to free radical-induced oxidative damage, and this may consequently lead to cellular ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm6110100
更新日期：2017-10-27 00:00:00
abstract：:Attention deficit hyperactivity disorder (ADHD) is a frequent neurodevelopmental disorder that often persists into adulthood. Methylphenidate (MPH) is the first-line treatment for ADHD; however, despite its wide usage, little is known about its neurometabolic effects. Until now, no randomized and blinded clinical tria...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9082601
更新日期：2020-08-11 00:00:00
abstract：:Anemia is a common feature of liver and bowel diseases. Although the main causes of anemia in these conditions are represented by gastrointestinal bleeding and iron deficiency, autoimmune hemolytic anemia should be considered in the differential diagnosis. Due to the epidemiological association, autoimmune hemolytic a...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm10030423
更新日期：2021-01-22 00:00:00
abstract：:The number of healthy and frail older adults is increasing worldwide, especially in industrialized countries, as a result of the increase in life expectancy [...]. ...
journal_title：Journal of clinical medicine
pub_type： 社论
doi：10.3390/jcm10020166
更新日期：2021-01-06 00:00:00
abstract：:Tuberculosis (TB) remains a major health issue worldwide. In order to contain TB infections, improved vaccines as well as accurate and reliable diagnostic tools are desirable. Exosomes are employed for the diagnosis of various diseases. At present, research on exosomes in TB is still at the preliminary stage. Recent s...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm10010145
更新日期：2021-01-04 00:00:00
abstract：:The recent advances in machine perfusion (MP) technology involve settings ranging between hypothermic, subnormothermic, and normothermic temperatures. Tissue level adenosine triphosphate (ATP) is a long-established marker of viability and functionality and is universal for all organs. In the midst of a growing number ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm8091421
更新日期：2019-09-09 00:00:00
abstract：:Background: Urinary calprotectin is a novel biomarker that distinguishes between intrinsic or prerenal acute kidney injury (AKI) in different studies. However, these studies were based on different populations and different AKI criteria. We evaluated the diagnostic accuracy of urinary calprotectin and compared its dia...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm8010074
更新日期：2019-01-10 00:00:00
abstract：:After decades of pioneering and improvement, kidney transplantation is now the renal replacement therapy of choice for most patients with end-stage kidney disease (ESKD). Where focus has traditionally been on surgical techniques and immunosuppressive treatment with prevention of rejection and infection in relation to ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9061911
更新日期：2020-06-18 00:00:00
abstract：:Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is an ultra-rare autosomal recessive disorder of nucleoside metabolism that is caused by mutations in the nuclear thymidine phosphorylase gene (TYMP) gene, encoding for the enzyme thymidine phosphorylase. There are currently no approved treatments for MNGIE...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8040457
更新日期：2019-04-05 00:00:00
abstract：:Cutaneous nerve entrapment plays an important role in neuropathic pain syndrome. Due to the advancement of ultrasound technology, the cutaneous nerves can be visualized by high-resolution ultrasound. As the cutaneous nerves course superficially in the subcutaneous layer, they are vulnerable to entrapment or collateral...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm7110457
更新日期：2018-11-21 00:00:00
abstract：BACKGROUND:To study the impact of MRI characteristics and of targeted biopsy (TB) core number on the final grade group (GG) prediction. MATERIALS AND METHODS:The cohort was 478 consecutive patients who underwent radical prostatectomy (RP) after positive mpMRI (multiparametric magnetic resonance imaging) followed by fu...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9010225
更新日期：2020-01-15 00:00:00
abstract：BACKGROUND:This study aimed to investigate the outcome of therapeutic penetrating keratoplasty (TPK) for medically-uncontrolled infectious keratitis, and to determine the factors associated with the recurrence of infection after TPK. METHODS:A 10-year retrospective study of medically-uncontrolled infectious keratitis ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9113696
更新日期：2020-11-18 00:00:00
abstract：:MicroRNAs (miRNAs) can provide insight into the pathophysiological states of ocular tissues such as proliferative diabetic retinopathy (PDR). In this study, differences in miRNA expression in vitreous from PDR patients with and without incidence of recurrent vitreous hemorrhage (RVH) after the initial pars-plana vitre...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8122217
更新日期：2019-12-15 00:00:00
abstract：:(1) Background: To examine trends in incidence and outcomes of urinary tract infections (UTIs) among men and women with or without chronic obstructive pulmonary disease (COPD), and to identify the predictors for in-hospital mortality (IHM). (2) Methods: We included patients (aged ≥40 years) who were hospitalized with ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9123979
更新日期：2020-12-09 00:00:00
abstract：:The diagnosis of small-bowel tumors is challenging due to their low incidence, nonspecific presentation, and limitations of traditional endoscopic techniques. In our study, we examined the utility of the mucosal protrusion angle in differentiating between true submucosal masses and bulges of the small bowel on video c...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8040418
更新日期：2019-03-27 00:00:00
abstract：:Studies have reported controversial findings on the association between fetal growth restriction (FGR) or intertwin weight discordance and the risk of hypertensive disorders of pregnancy (HDP) in twin pregnancies. The aim of this study was to investigate the association between twin growth disorders and HDP. Twin preg...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9103276
更新日期：2020-10-13 00:00:00
abstract：:Pediatric Adamantinomatous Craniopharyngiomas (ACPs) are histologically benign brain tumors that often follow an aggressive clinical course. Their suprasellar location leaves them in close proximity to critical neurological and vascular structures and often results in significant neuroendocrine morbidity. Current trea...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm9020519
更新日期：2020-02-14 00:00:00
abstract：:We investigated the prognostic influence of androgen receptor (AR) on breast cancer. AR status was assessed using immunohistochemistry with tissue microarrays from 395 operable primary breast cancer patients who received curative surgery. The Kaplan-Meier estimator was used to analyze the survival rates and a log-rank...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9041083
更新日期：2020-04-10 00:00:00
abstract：:This study assessed the efficacy of intraoperative high-dose intravenous iron therapy in facilitating recovery from postoperative anemia and reducing the transfusion rate in patients with total knee and total hip arthroplasty. This prospective randomized controlled study involved 58 subjects. Group F received 1000 mg ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8101674
更新日期：2019-10-13 00:00:00
abstract：:Atherosclerosis is a progressive, inflammatory cardiovascular disorder characterized by the development of lipid-filled plaques within arteries. Endothelial cell dysfunction in the walls of blood vessels results in an increase in vascular permeability, alteration of the components of the extracellular matrix, and rete...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
doi：10.3390/jcm8111856
更新日期：2019-11-02 00:00:00
abstract：:In recent years, the genetic architecture of dilated cardiomyopathy (DCM) has been more thoroughly elucidated. However, there is still insufficient knowledge on the modifiers and regulatory principles that lead to the failure of myocardial function. The current study investigates the association of epigenome-wide DNA ...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9051499
更新日期：2020-05-16 00:00:00
abstract：:We aimed to determine if low body weight (LBW) status (<50 kg) is independently associated with increased risk of ischemic stroke and bleeding in Thai patients with non-valvular atrial fibrillation (NVAF). (1) Background: It has been unclear whether LBW influence clinical outcome of patients with NVAF. (2) Methods: Th...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9092713
更新日期：2020-08-22 00:00:00
abstract：BACKGROUND:Currently, proposed nomograms are mainly based on post-operative histopathology. The purpose of this study was to identify preoperative computed tomography (CT) and clinical information that allow prediction of disease-free (DFS) and overall survival (OS) of patients surgically treated for pancreatic head ca...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8101749
更新日期：2019-10-21 00:00:00
abstract：:Branched-chain amino acid (BCAA) and insulin-like growth factor 1 (IGF-1) are essential for muscle protein synthesis. We investigated the association of serum BCAA and IGF-1 levels with sarcopenia and gait speed in 192 patients with liver cirrhosis (LC). Sarcopenia was diagnosed according to the Japan Society of Hepat...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm9103239
更新日期：2020-10-10 00:00:00
abstract：:The authors wish to make the following corrections to this paper [...]. ...
journal_title：Journal of clinical medicine
pub_type： 已发布勘误
doi：10.3390/jcm9020569
更新日期：2020-02-19 00:00:00
abstract：:Purpose: This study aimed to evaluate the effects of a child-centered exergaming program and a traditional teacher-led physical activity (PA) program on preschoolers' executive functions and perceived competence. Methods: Sixty children aged 4⁻5 years from an urban childcare center in China completed an 8-week exergam...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8040469
更新日期：2019-04-06 00:00:00
