Abstract:
BACKGROUND:This retrospective study evaluated the performance of a lipoarabinomannan (LAM)-based immunological method for diagnosing pleural tuberculosis (TB) from pleural effusion samples. Results were compared to those obtained using conventional culture and molecular testing methods. METHODS:Suspected pleural TB patients who visited Beijing Chest Hospital for medical care between January 2016 and June 2017 were retrospectively analysed in the study. Pleural effusion samples were tested for Mycobacterium tuberculosis (MTB) using the BACTEC MGIT 960 System, GeneXpert, and an anti-LAM antibody assay (LAM assay). RESULTS:Pleural effusion samples were collected from a total of 219 retrospectively recruited participants suspected of having pleural TB. Thirteen of 155 confirmed pleural TB cases tested positive for MTB via MGIT culture, for a sensitivity of 8.4% [95% confidence interval (CI): 4.0-12.8%]. In addition, GeneXpert and LAM testing identified 22 and 55 pleural TB cases, for sensitivities of 14.2% (95% CI: 8.7-19.7%) and 35.5% (95% CI: 28.1-43.6%), respectively. The specificities of these two assays were 100.0% (95% CI: 92.9-100.0%) and 96.9% (95% CI: 88.2-99.5%), respectively. Combined application of culture and LAM testing identified 60 positive cases, for a sensitivity of 38.7% (95% CI: 31.0-46.4%) that was significantly higher than that of MGIT culture alone (P < 0.01). Similarly, use of LAM testing in combination with GeneXpert led to correct diagnosis of 40.0% (95% CI: 32.3-47.7%) of pleural TB cases, a higher rate than obtained using GeneXpert alone (P < 0.01). In addition, the specificity of the combined assay of GeneXpert and LAM testing was 96.9% (95% CI: 88.2-99.5%). Patients aged 25 to 44 years were more likely to have positive LAM assay results than those ≥65 years of age (P = 0.02). Meanwhile, the proportion of diabetic patients with positive LAM assay results was significantly lower than that of the non-diabetes group (P = 0.03). CONCLUSIONS:An anti-LAM antibody detection assay showed potential for diagnosis of pleural TB from pleural effusion samples. Combined use of the LAM assay with MGIT culture or GeneXpert methods could improve sensitivity for improved pleural TB diagnosis compared to results of individual conventional tests alone.
journal_name
BMC Infect Disjournal_title
BMC infectious diseasesauthors
Liang Q,Pang Y,Yang Y,Li H,Guo C,Yang X,Chen Xdoi
10.1186/s12879-019-4166-1subject
Has Abstractpub_date
2019-06-21 00:00:00pages
548issue
1issn
1471-2334pii
10.1186/s12879-019-4166-1journal_volume
19pub_type
杂志文章abstract:BACKGROUND:Mycobacterium goodii is a rare cause of significant infection. M. goodii has mainly been associated with lymphadenitis, cellulitis, osteomyelitis, and wound infection. CASE PRESENTATION:A case of a 76-year-old Caucasian female is presented. The patient developed a prosthetic valve endocarditis caused by M. ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-12-301
更新日期:2012-11-14 00:00:00
abstract:BACKGROUND:Lassa fever has been a public health concern in the West African sub-region where it is endemic and a latent threat to the world at large. We investigated the trend in Lassa fever research using bibliometric approach. METHODS:We used the SCOPUS database employing "Lassa fever" as search descriptor. The most...
journal_title:BMC infectious diseases
pub_type: 历史文章,杂志文章
doi:10.1186/s12879-018-3526-6
更新日期:2018-12-10 00:00:00
abstract:BACKGROUND:Carbapenemase-producing organisms (CPOs) have emerged as antibiotic-resistant bacteria of global concern. Here we assessed the performance of the Carba (beta) assay, a multiplex real-time PCR assay developed by SpeeDx for the detection of key carbapenemase-encoding genes: KPC, NDM, OXA-48-like, IMP-4-like, a...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4176-z
更新日期:2019-07-02 00:00:00
abstract:BACKGROUND:Models of infectious disease are increasingly utilising empirical contact data to quantify the number of potentially infectious contacts between age groups. While a growing body of data is being collected on contact patterns across many populations, less attention has been paid to the social contacts of youn...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-017-2735-8
更新日期:2017-09-20 00:00:00
abstract:BACKGROUND:No comparison data have been reported on viral and epidemiological profiles of hospitalized children with severe acute respiratory infection (SARI) in Beijing or Shanghai, China. METHODS:We collected 700 nasopharyngeal aspirates (NPA) from hospitalized children with SARI in Beijing (northern China) and Shan...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4385-5
更新日期:2019-08-20 00:00:00
abstract:BACKGROUND:Poor outcomes of invasive candidiasis (IC) are associated with the difficulty in establishing the microbiological diagnosis at an early stage. New scores and laboratory tests have been developed in order to make an early therapeutic intervention in an attempt to reduce the high mortality associated with inva...
journal_title:BMC infectious diseases
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2334-11-60
更新日期:2011-03-09 00:00:00
abstract:BACKGROUND:How HIV-1 enter into the eyes remains obscure. We postulated that HIV-1 Tat protein can alter the expression of specific tight-junction proteins and disturb the blood retinal barrier, and contributes to HIV trafficking into the eyes. This study is to determine the effects of HIV-1 Tat proteins on the barrier...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-8-77
更新日期:2008-06-06 00:00:00
abstract:BACKGROUND:Viral load (VL) testing is being scaled up in resource-limited settings. However, not all commercially available VL testing methods have been evaluated under field conditions. This study is one of a few to evaluate the Biocentric platform for VL quantification in routine practice in Sub-Saharan Africa. METH...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3474-1
更新日期:2018-11-14 00:00:00
abstract:BACKGROUND:Tuberculosis (TB)-associated Immune reconstitution inflammatory syndrome (TB-IRIS) is an aberrant inflammatory response in TB patients with advanced human immunodeficiency virus coinfection, after antiretroviral therapy commencement. CASE PRESENTATION:We present a rare case of a 51-year-old woman living wit...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4182-1
更新日期:2019-06-17 00:00:00
abstract:BACKGROUND:After the onset of HAART, some HIV-infected individuals under treatment present a exacerbated inflammation in response to a latent or a previously treated opportunistic pathogen termed immune reconstitution inflammatory syndrome (IRIS). Few reports of tegumentary leishmaniasis have been described in associat...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-0774-6
更新日期:2015-02-03 00:00:00
abstract:BACKGROUND:Routine culture-based diagnosis of Pseudomonas aeruginosa lung infection in Cystic Fibrosis (CF) patients can be hampered by the phenotypic variability of the microorganism, including its transition to a Viable But Non-Culturable (VBNC) state. The aim of this study was to validate an ecfX-targeting qPCR prot...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3612-9
更新日期:2018-12-27 00:00:00
abstract:BACKGROUND:Infection by Staphylococcus aureus (S. aureus) is a major cause of morbidity and mortality. Colonization by S. aureus increases the risk of infection. Little is known about decolonization strategies for S. aureus beyond antibiotics, however probiotics represent a promising alternative. A randomized controlle...
journal_title:BMC infectious diseases
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12879-018-3028-6
更新日期:2018-03-14 00:00:00
abstract:BACKGROUND:Dengue is the most widespread mosquito-borne viral disease of public health concern. In some patients, endothelial cell and platelet dysfunction lead to life-threatening hemorrhagic dengue fever or dengue shock syndrome. Prognostication of disease severity is urgently required to improve patient management. ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-1271-7
更新日期:2015-11-14 00:00:00
abstract:BACKGROUND:Alcohol use has a detrimental impact on the HIV epidemic, especially in sub-Saharan Africa. HIV counseling and testing (HCT) may provide a contact opportunity to intervene with hazardous alcohol use; however, little is known about how alcohol consumption changes following HCT. METHODS:We utilized data from ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-14-403
更新日期:2014-07-20 00:00:00
abstract:BACKGROUND:The present study aims to investigate the potential mechanism of linezolid-resistant Staphylococcus capitis (LRSC) isolates collected from our hospital. METHODS:The susceptibilities of 5 Staphylococcus capitis isolates displaying resistance towards linezolid were determined by E-test. Polymerase chain react...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-0841-z
更新日期:2015-02-26 00:00:00
abstract:BACKGROUND:To determine the prevalence of enteric infections in Aboriginal children aged 0-2 years using conventional and molecular diagnostic techniques and to explore associations between the presence of pathogens and child growth. METHODS:Cross-sectional analysis of Aboriginal children (n = 62) residing in a remote...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05685-1
更新日期:2021-01-06 00:00:00
abstract:BACKGROUND:NOD2, an intracellular pathogen recognition sensor, modulates innate defences to muropeptides derived from various bacterial species, including Mycobacterium tuberculosis (MTB). Experimentally, NOD2 attenuates two key putative mycobactericidal mechanisms. TNF-alpha synthesis is markedly reduced in MTB-antige...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-7-96
更新日期:2007-08-16 00:00:00
abstract:BACKGROUND:Several highly pathogenic avian influenza (AI) outbreaks have been reported over the past decade. South Korea recently faced AI outbreaks whose economic impact was estimated to be 6.3 billion dollars, equivalent to nearly 50% of the profit generated by the poultry-related industries in 2008. In addition, AI ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-10-236
更新日期:2010-08-10 00:00:00
abstract:BACKGROUND:Typhoid fever mainly occurs in (sub) tropical regions where sanitary conditions remain poor. In other regions it occurs mainly among returning travelers or their direct contacts. The aim of this study was to evaluate the current Dutch guidelines for typhoid vaccination. METHOD:Crude annual attack rates (AR)...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-2059-0
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND:Non-organ-specific autoantibodies are found in a considerable number of anti-HCV positive patients. Previous studies investigated the clinical relevance of these antibodies in patients treated with interferon monotherapy, but not combination therapies. METHODS:Anti-nuclear, anti-smooth muscle, anti-mitochon...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-4-4
更新日期:2004-02-13 00:00:00
abstract:BACKGROUND:H7N9 posed potentially serious health challenges for Chinese society. The previous SARS outbreak in this country was accompanied by contradictory information, while worries about wide-spread influenza led to discrimination worldwide. Early understanding of public threat perceptions is therefore important for...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-14-8
更新日期:2014-01-07 00:00:00
abstract::After publication of the original article [1], we were notified that an author's name has been incorrectly spelled. Jeff Kwong's full name is Jeffery C. Kwong. ...
journal_title:BMC infectious diseases
pub_type: 已发布勘误
doi:10.1186/s12879-019-4579-x
更新日期:2019-10-24 00:00:00
abstract:BACKGROUND:The swine influenza H1N1 first identified in Mexico, spread rapidly across the globe and is considered the fastest moving pandemic in history. The early phase of an outbreak, in which data is relatively scarce, presents scientific challenges on key issues such as: scale, severity and immunity which are funda...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-11-92
更新日期:2011-04-14 00:00:00
abstract:BACKGROUND:Variations of porB1A and porB1B genes and their serotypes exist in Neisseria gonorrhoeae isolates from different geographical areas, and some site mutations in the porB1B gene correlate with drug resistance. METHODS:The β-lactamase production of N. gonorrhoeae isolates was determined by paper acidometric te...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-10-323
更新日期:2010-11-10 00:00:00
abstract:BACKGROUND:Chronic osteomyelitis due to direct bone trauma or vascular insufficiency is a frequent problem in orthopaedic surgery. In contrast, acute haematogenous osteomyelitis represents a rare entity that almost exclusively affects prepubescent children or immunodeficient adults. CASE PRESENTATION:In this article, ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-13-266
更新日期:2013-06-05 00:00:00
abstract:BACKGROUND:Varicella zoster virus (VZV) encephalitis is an infectious inflammatory disease of brain that can cause irreversible mental damage without timely treatment. In fact, many viruses can cause encephalitis, and the viral loads in cerebrospinal fluid (CSF) in the early stage of the disease are usually too low to ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-3986-3
更新日期:2019-04-25 00:00:00
abstract:BACKGROUND:Antibiotic resistance is a threat in developing countries (DCs) because of the high burden of bacterial disease and the presence of risk factors for its emergence and spread. This threat is of particular concern for neonates in DCs where over one-third of neonatal deaths may be attributable to severe infecti...
journal_title:BMC infectious diseases
pub_type: 杂志文章,评审
doi:10.1186/s12879-015-0843-x
更新日期:2015-03-15 00:00:00
abstract:BACKGROUND:Hantavirus infection is worldwide epidemic and can cause life-threatening consequences. With more and more cases reported in countries with atypical morbidity, it is necessarily urgent to know some atypical symptoms and signs of Hantavirus infection. CASE PRESENTATION:Here we report the case of a 44-year ol...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-4775-8
更新日期:2020-01-13 00:00:00
abstract:BACKGROUND:Chlamydia trachomatis is the most common sexually transmitted bacterial pathogen causing female genital tract infection throughout the world. Reinfection with the same serovar, as well as multiple infections with different serovars, occurs in humans. Using a murine model of female C. trachomatis genital trac...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-5-105
更新日期:2005-11-17 00:00:00
abstract:BACKGROUND:Syndromic surveillance is increasingly being evaluated for its potential for early warning of increased disease activity in the population. However, interpretation is hampered by the difficulty of attributing a causative pathogen. We described the temporal relationship between laboratory counts of influenza ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-9-190
更新日期:2009-11-29 00:00:00