Abstract:
BACKGROUND:Carbapenemase-producing organisms (CPOs) have emerged as antibiotic-resistant bacteria of global concern. Here we assessed the performance of the Carba (beta) assay, a multiplex real-time PCR assay developed by SpeeDx for the detection of key carbapenemase-encoding genes: KPC, NDM, OXA-48-like, IMP-4-like, and VIM. METHODS:DNA extracts of 180 isolates were tested with the Carba (beta) assay, using previously validated in-house TaqMan probe assays for the relevant carbapenemase genes as the reference standard. The Carba (beta) assay was then directly used to screen 460 DNA extracts of faecal specimens, with positive results subjected to the aforementioned in-house assays plus Sanger sequencing. RESULTS:The Carba (beta) assay correctly identified the presence of the respective carbapenemase genes in 154 of 156 isolates and provided negative results for all 24 non-CPO isolates. Two isolates provided positive results for OXA-48-like carbapenemase by the Carba (beta) assay only. The Carba (beta) assay had sensitivities of 100% for all targets, and specificities of 100% for KPC, NDM, IMP-4-like, and VIM targets, and 98.5% for OXA-48-like targets. When applied directly to faecal specimens, eight samples were positive by the Carba (beta) assay, two of which were confirmed by in-house TaqMan probe PCR or DNA sequencing. CONCLUSIONS:The Carba (beta) assay is highly sensitive and specific for detecting key carbapenemase genes in isolates. Further testing is required to assess this assay's suitability for direct screening of clinical specimens.
journal_name
BMC Infect Disjournal_title
BMC infectious diseasesauthors
Bordin A,Trembizki E,Windsor M,Wee R,Tan LY,Buckley C,Syrmis M,Bergh H,Cottrell K,Zowawi HM,Sidjabat HE,Harris PNA,Nimmo GR,Paterson DL,Whiley DMdoi
10.1186/s12879-019-4176-zsubject
Has Abstractpub_date
2019-07-02 00:00:00pages
571issue
1issn
1471-2334pii
10.1186/s12879-019-4176-zjournal_volume
19pub_type
杂志文章abstract:BACKGROUND:The most optimal antifungal agent for empiric treatment of invasive fungal diseases (IFDs) in febrile neutropenia is controversial. Our objective was evaluate the relative efficacy of antifungals for all-cause mortality, fungal infection-related mortality and treatment response in this population. METHODS:P...
journal_title:BMC infectious diseases
pub_type: 杂志文章,meta分析,评审
doi:10.1186/s12879-017-2263-6
更新日期:2017-02-20 00:00:00
abstract:BACKGROUND:Preliminary results suggest that pertussis infection might be considered in infants during a seasonal respiratory syncytial virus (RSV) outbreak. METHODS:In order to analyze clinical features and laboratory findings in infants with pertussis hospitalized for acute respiratory symptoms during a seasonal RSV ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-13-526
更新日期:2013-11-08 00:00:00
abstract:BACKGROUND:Long-term outcome of DAAs therapy in kidney transplant recipients was unknown. Thus, we aimed to evaluate it in a Chinese cohort of HCV-infected kidney transplant recipients. METHODS:Single-center and retrospective study of HCV-infected kidney transplant recipients initiating an DAAs regimen between January...
journal_title:BMC infectious diseases
pub_type: 临床试验,杂志文章
doi:10.1186/s12879-019-4217-7
更新日期:2019-07-19 00:00:00
abstract:BACKGROUND:Tuberculosis (TB) is a major public health problem in developing countries like Bangladesh. Female sex workers (FSWs) and their clients are active sources for spreading TB. The purpose of this study was to assess the knowledge of TB among FSWs in Rajshahi city, Bangladesh. METHODS:It was a cross-sectional s...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4531-0
更新日期:2019-10-11 00:00:00
abstract:BACKGROUND:Identification of the causative agents of invasive fungal infections (IFI) is critical for guiding antifungal therapy. Cultures remain negative in a substantial number of IFI cases. Accordingly, species identification from formalin fixed, paraffin embedded (FFPE) tissue specimens by molecular methods such as...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-11-202
更新日期:2011-07-26 00:00:00
abstract:BACKGROUND:The Global Polio Eradication Initiative plans for coordinated cessation of oral poliovirus vaccine (OPV) use, beginning with serotype 2-containing OPV (i.e., OPV2 cessation) followed by the remaining two OPV serotypes (i.e., OPV13 cessation). The risk of circulating vaccine-derived poliovirus (cVDPV) outbrea...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-1114-6
更新日期:2015-09-24 00:00:00
abstract:BACKGROUND:Streptococcus pneumoniae is a leading cause of morbidity and mortality in the elderly. To prevent invasive pneumococcal diseases, the 23-valent pneumococcal polysaccharide vaccine (PPV) is recommended in subjects over 65 years of age. Although it has been reported to provide approximately 50-80% protection a...
journal_title:BMC infectious diseases
pub_type: 临床试验,杂志文章
doi:10.1186/1471-2334-10-60
更新日期:2010-03-10 00:00:00
abstract:BACKGROUND:A novel Ehrlichia, closely related to Ehrlichia ruminantium, was recently discovered from Panola Mountain State Park, GA, USA. We conducted a study to determine if this agent was recently introduced into the United States. METHODS:We developed a sensitive PCR assay based on the conserved gltA (citrate synth...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-8-54
更新日期:2008-04-23 00:00:00
abstract:BACKGROUND:Central nervous system (CNS) infections are a significant contributor to morbidity and mortality globally. However, most published studies have been conducted in developed countries where the epidemiology and aetiology differ significantly from less developed areas. Additionally, there may be regional differ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1640-x
更新日期:2016-06-16 00:00:00
abstract:BACKGROUND:Dose-response assessment is one step in quantitative microbial risk assessment (QMRA). Four infectious microbes capable of causing respiratory diseases important to public health, and for which dose-response functions have not been available are: Bordetella pertussis (whooping cough), group A Streptococcus (...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-0832-0
更新日期:2015-02-24 00:00:00
abstract:BACKGROUND:HIV affects the central nervous system resulting in HIV associated neurocognitive impairment (NCI) in approximately 50% of people living with HIV. It typically affects memory, learning, working memory, fine motor skills, speed of information processing, verbal fluency and executive functioning cognitive doma...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05090-8
更新日期:2020-05-29 00:00:00
abstract:BACKGROUND:Infections by Streptococcus gallolyticus subsp. pasteurianus (SGSP) is often underestimated. Herein, the epidemiological features and resistant characteristics of SGSP in mainland China are characterized to enable a better understanding of its role in clinical infections. METHODS:In the present work, 45 SGS...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4413-5
更新日期:2019-09-09 00:00:00
abstract:BACKGROUND:We have recovered one bla(NDM-1)-harboring bacterial strain, designated as XM1570, from a sputum sample obtained from a fatal case of pneumonia in China. METHODS:Biochemical profiling, 16S rRNA sequencing and antimicrobial susceptibility testing were performed. Conjugation experiments were conducted to dete...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-0870-7
更新日期:2015-03-18 00:00:00
abstract:BACKGROUND:Vitamin D enhances host protective immune responses to Mycobacterium tuberculosis by suppressing Interferon-gamma (IFN-g) and reducing disease associated inflammation in the host. The objectives of this study were to determine whether vitamin D supplementation to patients with tuberculosis (TB) could influen...
journal_title:BMC infectious diseases
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1186/1471-2334-13-22
更新日期:2013-01-19 00:00:00
abstract:BACKGROUND:Diseases caused by Mycobacterium tuberculosis (TB) among adult patients with hematological malignancies have rarely been investigated. METHODS:Adult patients with hematological malignancies at National Taiwan University Hospital between 1996 and 2009 were retrospectively reviewed. Patients with positive ser...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-11-324
更新日期:2011-11-23 00:00:00
abstract:BACKGROUND:Clinically mild encephalitis/encephalopathy with a reversible splenial lesion (MERS) is a mild encephalopathy caused by various pathological processes, but encephalopathy due to bacteria is rare. CASE PRESENTATION:We report the case of a 45-year-old Japanese woman who on receiving chemotherapy for advanced ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1516-0
更新日期:2016-04-18 00:00:00
abstract:BACKGROUND:The accuracy of a new optical biosensor (OB) point-of-care device for the detection of severe infections is studied. METHODS:The OB emits different wavelengths and outputs information associated with heart rate, pulse oximetry, levels of nitric oxide and kidney function. At the first phase, recordings were ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05607-1
更新日期:2020-11-19 00:00:00
abstract:BACKGROUND:Anisakid nematodes (Anisakis spp. or Pseudoterranova spp.) usually infect gastric or intestinal walls, while they rarely infect in extra-gastrointestinal sites of human body. Generally, Anisakis spp. larvae are highly infected in fish intermediate hosts, whereas Pseudoterranova spp. larvae are very rarely in...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3540-8
更新日期:2018-12-04 00:00:00
abstract:BACKGROUND:Melioidosis has become an emerging infection in Sri Lanka; a country which is considered non endemic for it. Paraplegia due to Burkholderia pseudomallei is a very rare entity encountered even in countries where the disease is endemic. There are no reported cases of transverse myelitis due to melioidosis in S...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-12-232
更新日期:2012-09-28 00:00:00
abstract:BACKGROUND:Trichosporon mycotoxinivorans is a recently described yeast-like fungal organism and its association as a pathogen for patients with cystic fibrosis (CF) was reported previously. We show the clinical course of a CF patient over 9 years as well as the applications of modern molecular and proteomic identificat...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1910-7
更新日期:2016-10-25 00:00:00
abstract:BACKGROUND:Non-severe acute respiratory syndrome (non-SARS)-related human coronaviruses (HCoVs), including HCoV-229E, -HKU1, -NL63, and -OC43, have been detected in respiratory tract samples from children and adults. However, the natural prevalence of antibodies against these viruses in serum among population is unknow...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-13-433
更新日期:2013-09-16 00:00:00
abstract:BACKGROUND:With ageing of the HIV-infected population, long-term exposure to treatment, varying adherence, emerging resistance and complications to therapies, effectiveness of Highly Active Antiretroviral Therapy (HAART) needs to be monitored continuously at the population level. The German HIV-1 Seroconverter Cohort i...
journal_title:BMC infectious diseases
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2334-12-94
更新日期:2012-04-19 00:00:00
abstract:BACKGROUND:Data regarding HIV-seronegative pediatric patients with cryptococcal meningitis (CM) have been very limited. METHODS:We retrospectively reviewed non-HIV-infected in patients with CM from January 2002 through December 2013 in Beijing Children's Hospital. Records of the all patients were obtained and compared...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1964-6
更新日期:2016-11-04 00:00:00
abstract:BACKGROUND:Rubella infection in pregnant women can result in serious effects, such as miscarriages, stillbirths, and congenital rubella syndrome (CRS). However, very little is known about the rubella seroprevalence among pregnant women in China. METHODS:This is a cross-sectional and hospital-based study. From June 201...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3032-x
更新日期:2018-03-15 00:00:00
abstract:BACKGROUND:Direct agglutination test (DAT) as a simple, accurate and reliable method, has been widely used for serodiagnosis of visceral leishmaniasis (VL) during the last three decades. The present study is a systematic review and meta-analysis to evaluate the diagnostic accuracy of DAT for serodiagnosis of human VL. ...
journal_title:BMC infectious diseases
pub_type: 杂志文章,meta分析
doi:10.1186/s12879-020-05558-7
更新日期:2020-12-11 00:00:00
abstract:BACKGROUND:Brucella abortus is a highly pathogenic zoonotic agent, tempting for the development of a rapid diagnostic method to enable adequate treatment and prevent further spread. Enrichment of the bacteria is often used as a first step in diagnostics to increase the bacterial number above the detection limit of the ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3134-5
更新日期:2018-06-05 00:00:00
abstract:BACKGROUND:In vitro data suggests that suboptimal initial vancomycin exposure may select for heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) infections. However, no clinical studies have evaluated the relationship between initial vancomycin exposure and emergence of hVISA. This pilot study seeks to ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-017-2609-0
更新日期:2017-08-02 00:00:00
abstract:BACKGROUND:Kazakhstan remains a high-burden TB prevalence country with a concomitent high-burden of multi-drug resistant tuberculosis. For this reason, we performed an in depth genetic diversity and population structure characterization of Mycobacterium tuberculosis complex (MTC) genetic diversity in Kazakhstan with bo...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4201-2
更新日期:2019-06-24 00:00:00
abstract:BACKGROUND:The human major histocompatibility complex class I polypeptide-related sequence B (MICB) is a protein that modulates the NK and T cell activation through the NKG2D receptor and is related to several diseases including cancer. METHODS:The study investigated the prognostic role of soluble MICB (sMICB) protein...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-0754-x
更新日期:2015-01-23 00:00:00
abstract:BACKGROUND:Australia is developing a chlamydia screening program. This study aimed to determine the attitudes of young women to the introduction of chlamydia screening in Australian General Practice. METHODS:In-depth face-to-face interviews with 24 young women from across Victoria, Australia, attending a randomly sele...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-8-62
更新日期:2008-05-09 00:00:00