Clonal dissemination of linezolid-resistant Staphylococcus capitis with G2603T mutation in domain V of the 23S rRNA and the cfr gene at a tertiary care hospital in China.

Abstract:

BACKGROUND:The present study aims to investigate the potential mechanism of linezolid-resistant Staphylococcus capitis (LRSC) isolates collected from our hospital. METHODS:The susceptibilities of 5 Staphylococcus capitis isolates displaying resistance towards linezolid were determined by E-test. Polymerase chain reactions (PCRs) and DNA sequencing were used to investigate the potential molecular mechanism. Clonal relatedness between these strains was analyzed by pulsed-field gel electrophoresis (PFGE). RESULTS:The MICs of linezolid on these 5 isolates were >256 μg/mL. The G2603T mutation was observed in the domain V of the 23S rRNA with cfr gene being also widely detected among these 5 strains. PFGE analysis displayed close genetic relatedness between these linezolid-resistant isolates. CONCLUSIONS:The emergence of LRSC isolates carrying G2603T mutation in the domain V of the 23S rRNA and harboring cfr gene in our hospital may pose a potential challenge to the public health.

journal_name

BMC Infect Dis

journal_title

BMC infectious diseases

authors

Zhou W,Niu D,Cao X,Ning M,Zhang Z,Shen H,Zhang K

doi

10.1186/s12879-015-0841-z

subject

Has Abstract

pub_date

2015-02-26 00:00:00

pages

97

issn

1471-2334

pii

10.1186/s12879-015-0841-z

journal_volume

15

pub_type

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