Abstract:
BACKGROUND:Viral load (VL) testing is being scaled up in resource-limited settings. However, not all commercially available VL testing methods have been evaluated under field conditions. This study is one of a few to evaluate the Biocentric platform for VL quantification in routine practice in Sub-Saharan Africa. METHODS:Venous blood specimens were obtained from patients eligible for VL testing at two health facilities in Swaziland from October 2016 to March 2017. Samples were centrifuged at two laboratories (LAB-1, LAB-2) to obtain paired plasma specimens for VL quantification with the national reference method and on the Biocentric platform. Agreement (correlation, Bland-Altman) and accuracy (sensitivity, specificity) indicators were calculated at the VL thresholds of 416 (2.62 log10) and 1000 (3.0 log10) copies/mL. Leftover samples from patients with discordant VL results were re-quantified and accuracy indicators recalculated. Logistic regression was used to compare laboratory performance. RESULTS:A total of 364 paired plasma samples (LAB-1: n = 198; LAB-2: n = 166) were successfully tested using both methods. The correlation was high (R = 0.82, p < 0.01), and the Bland-Altman analysis showed a minimal mean difference (- 0.03 log10 copies/mL; 95% CI: -1.15 to 1.08). At the clinical threshold level of 3.0 log10 copies/mL, the sensitivity was 88.6% (95% CI: 78.7 to 94.9) and the specificity was 98.3% (95% CI: 96.1 to 99.4). Sensitivity was higher in LAB-1 (100%; 95% CI: 71.5 to 100) than in LAB-2 (86.4%; 95% CI: 75.0 to 94.0). Most upward (n = 8, 2.2%) and downward (n = 11, 3.0%) misclassifications occurred at the 2.62 log threshold, with LAB-2 having a 16 (95% CI: 2.26 to 113.27; p = 0.006) times higher odds of downward misclassification. After retesting of discordant leftover samples (n = 17), overall sensitivity increased to 93.5% (95% CI: 85.5 to 97.9) and 97.1% (95% CI: 90.1 to 99.7) at the 2.62 and 3.0 thresholds, and specificity increased to 98.6% (95% CI: 96.5 to 99.6) and 99.0% (95% CI: 97.0 to 99.8) respectively. CONCLUSIONS:The test characteristics of the Biocentric platform were overall comparable to the national reference method for VL quantification. One laboratory tended to misclassify VL results downwards, likely owing to unmet training needs and lack of previous hands-on practice.
journal_name
BMC Infect Disjournal_title
BMC infectious diseasesauthors
Kerschberger B,Mpala Q,Uribe PAD,Maphalala G,de la Tour R,Kalombola S,Bekele A,Chawinga T,Mliba M,Ntshalintshali N,Phugwayo N,Kabore SM,Goiri J,Dlamini S,Ciglenecki I,Fajardo Edoi
10.1186/s12879-018-3474-1subject
Has Abstractpub_date
2018-11-14 00:00:00pages
570issue
1issn
1471-2334pii
10.1186/s12879-018-3474-1journal_volume
18pub_type
杂志文章abstract:BACKGROUND:Restricted use of third-generation cephalosporins and fluoroquinolones has been linked to a reduced incidence of hospital-acquired infections with multidrug-resistant bacteria. We implemented an intensified antibiotic stewardship (ABS) programme in the medical service of a university hospital center aiming a...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-14-201
更新日期:2014-04-15 00:00:00
abstract:BACKGROUND:During the fifth wave of human H7N9 infections, a novel highly pathogenic (HP) H7N9 variant emerged with an insertion of multiple basic amino acids in the HA cleavage site. Moreover, a neuraminidase inhibitor (NAI) resistance (R292K in NA) mutation was found in H7N9 isolates from humans, poultry and the envi...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3302-7
更新日期:2018-08-15 00:00:00
abstract:BACKGROUND:Granulysin (GNLY) is produced by human lymphocyte subpopulations and exhibits antimicrobial activity against Mycobacterium tuberculosis. We examined the association between GNLY levels in blood and latent tuberculosis (TB) infection. METHODS:Latency of TB infection among Vietnamese healthcare workers was es...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1911-6
更新日期:2016-10-18 00:00:00
abstract:BACKGROUND:There is paucity of data on seasonal variation in pulmonary tuberculosis (TB) in developing countries contrary to recognized seasonality in the TB notification in western societies. This study examined the seasonal pattern in TB diagnosis among migrant workers from developing countries entering Kuwait. METH...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-8-3
更新日期:2008-01-07 00:00:00
abstract:BACKGROUND:We compared AIDS-related mortality rates in people living with HIV (PLHIV) starting antiretroviral therapy (ART) in Brazil during 2006-2015 and examined associated risk factors . METHODS:Data on ART use in PLHIV and AIDS mortality in Brazil was analysed with piecewise constant exponential models. Mortality ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-3844-3
更新日期:2019-02-28 00:00:00
abstract:BACKGROUND:Leprosy still remains an important public health problem for many parts of the world. An association of gangrene with leprosy is a rare one & can have a number of causative mechanisms. We present a case with Leprosy & gangrene with positive anti phopholipid antibody titers. CASE PRESENTATION:A 50-year-old n...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-5-74
更新日期:2005-09-21 00:00:00
abstract:BACKGROUND:There are inconsistencies in the literature on reproductive and genital health determinants of human papillomavirus (HPV) infection, the primary cause of cervical cancer. We examined these factors in the Ludwig-McGill Cohort Study, a longitudinal, repeated-measurements investigation on the natural history of...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1446-x
更新日期:2016-03-08 00:00:00
abstract:BACKGROUND:Tuberculosis (TB) is a major public health problem in developing countries like Bangladesh. Female sex workers (FSWs) and their clients are active sources for spreading TB. The purpose of this study was to assess the knowledge of TB among FSWs in Rajshahi city, Bangladesh. METHODS:It was a cross-sectional s...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4531-0
更新日期:2019-10-11 00:00:00
abstract:BACKGROUND:It is widely assumed that voluntary counselling and testing (VCT) services contribute to HIV prevention by motivating clients to reduce sexual risk-taking. However, findings from sub-Saharan Africa have been mixed, particularly among HIV-negative persons. We explored associations between VCT use and changes ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-14-159
更新日期:2014-03-22 00:00:00
abstract:BACKGROUND:Despite the successes of routine national childhood vaccination programmes, measles remains a public health concern. The purpose of this paper is to investigate how patterns of parental attitudes are linked to the decision-making process for or against MMR vaccination. This exploratory study was designed to ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1747-0
更新日期:2016-08-11 00:00:00
abstract:BACKGROUND:A cost effective and efficient diagnostic tool for COVID-19 as near to the point of care (PoC) as possible would be a game changer in the current pandemic. We tested reverse transcription loop mediated isothermal amplification (RT-LAMP), a method which can produce results in under 30 min, alongside standard ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05484-8
更新日期:2020-10-20 00:00:00
abstract:BACKGROUND:The East African region is endemic with multiple zoonotic diseases and is one of the hotspots for emerging infectious zoonotic diseases with reported multiple outbreaks of epidemic diseases such as Ebola, Marburg and Rift Valley Fever. Here we present a systematic assessment of published research on zoonotic...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3443-8
更新日期:2018-11-03 00:00:00
abstract:BACKGROUND:The risk of life-threatening complications, such as visceral disseminated varicella zoster virus (VZV) infection, is greater in immunosuppressed individuals, such as systemic lupus erythematosus (SLE) patients. CASE PRESENTATION:Here, a case is reported of a Caucasian woman diagnosed with lupus nephritis an...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05254-6
更新日期:2020-07-23 00:00:00
abstract:BACKGROUND:Tétanos Quick Stick® (TQS) is a test for tetanus immunity screening for wounded patients in emergency departments (EDs), but represents additional costs compared with a medical interview on vaccination history. The study objective was to assess the effectiveness and cost of the TQS in French EDs. METHODS:We...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-014-0603-3
更新日期:2014-11-19 00:00:00
abstract:BACKGROUND:There currently is a severe Zika Virus (ZIKV) epidemic in Brazil and other South American countries. Due to international travel, this poses severe public health risk of ZIKV importation to other countries. We estimate the prevalence of ZIKV in an import region by the time a microcephaly case is detected, si...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-2076-z
更新日期:2016-12-12 00:00:00
abstract:BACKGROUND:Abnormal sexual behaviors presenting as manifestations of rabies have occasionally been reported in the literature, although little attention has been paid to these cases to date. This study aimed to analyze the clinical features of rabies cases with abnormal sexual behaviors as the presenting manifestations...
journal_title:BMC infectious diseases
pub_type: 杂志文章,评审
doi:10.1186/s12879-019-4252-4
更新日期:2019-08-01 00:00:00
abstract:BACKGROUND:The objective of this study was to describe the clinical and microbiological characteristics of bloodstream infections (BSIs) due to AmpC producing Enterobacteriaceae (AE) in a large centralized Canadian region over a 9-year period. METHODS:An active surveillance cohort design in Calgary, Canada. RESULTS:A...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-014-0647-4
更新日期:2014-12-14 00:00:00
abstract:BACKGROUND:Cryptococcus neoformans causes meningitis and disseminated infection in healthy individuals, but more commonly in hosts with defective immune responses. Cell-mediated immunity is an important component of the immune response to a great variety of infections, including yeast infections. We aimed to evaluate a...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-12-278
更新日期:2012-10-30 00:00:00
abstract:BACKGROUND:Contact tracing of tuberculosis (TB) patients is rarely performed in low-income countries. Our objective was to assess the outcome of and compliance with directly observed treatment (DOTS) of TB patients over a 3 year period in rural Lao PDR. METHODS:We performed a retrospective cohort study in which we enr...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-10-265
更新日期:2010-09-10 00:00:00
abstract:BACKGROUND:The widespread antimicrobial resistance of Neisseria gonorrhoeae is a serious problem for the treatment and control of gonorrhoea. Many of the previously effective therapeutic agents are no longer viable. Because N. gonorrhoeae infections are not reportable in Germany, only limited data on disease epidemiolo...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-2944-9
更新日期:2018-01-17 00:00:00
abstract:BACKGROUND:Recent evidence indicates that Gram-negative bacterial pathogens, the most common of which are Pseudomonas spp., Enterobacteriaceae, and Acinetobacter baumannii, are frequent causes of hospital-acquired infections. This study aims to evaluate the in vitro activity of doripenem and comparator carbapenem antib...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-12-42
更新日期:2012-02-16 00:00:00
abstract:BACKGROUND:Amphotericin-induced phlebitis is a common infusion-related reaction in patients managed for cryptococcal meningitis. High-quality nursing care is critical component to successful cryptococcosis treatment. We highlight the magnitude and main approaches in the management of amphotericin-induced phlebitis and ...
journal_title:BMC infectious diseases
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1186/s12879-019-4209-7
更新日期:2019-06-26 00:00:00
abstract:BACKGROUND:The association of Chlamydia pneumoniae with atherosclerosis is controversial. We investigated the presence of C. pneumoniae and other Chlamydia spp. in atheromatous carotid artery tissue. METHODS:Forty elective carotid endarterectomy patients were recruited (27 males, mean age 65 and 13 females mean age 68...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-1-12
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Drug susceptibility testing (DST) remains an important concern for implementing treatment of MDR tuberculosis patients. Implementation of molecular tests for drug resistance identification would facilitate DST particularly in developing countries where culturing is difficult to perform. We have characterized...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-11-255
更新日期:2011-09-28 00:00:00
abstract:BACKGROUND:Helminth infections can alter susceptibility to malaria. Studies need to determine whether or not deworming programs can impact on Plasmodium infections in preschool children. METHODS:A double-blind placebo-controlled randomised trial was conducted to investigate the impact of anthelmintic treatment on Plas...
journal_title:BMC infectious diseases
pub_type: 杂志文章,随机对照试验
doi:10.1186/1471-2334-10-277
更新日期:2010-09-21 00:00:00
abstract:BACKGROUND:PCRctic is an innovative assay based on 16S rDNA PCR technology that has been designed to detect a single intact bacterium in a specimen of cerebro-spinal fluid (CSF). The assay's potential for accurate, fast and inexpensive discrimination of bacteria-free CSF makes it an ideal adjunct for confident exclusio...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05160-x
更新日期:2020-06-22 00:00:00
abstract:BACKGROUND:Compared to methicillin-resistant Staphylococcus aureus (MRSA), characteristics of nasal carriage and community-onset infection methicillin-susceptible S. aureus (MSSA) are less well known. No characteristics of MSSA in Taiwan have been reported previously. METHODS:We analyzed 100 nasal carriage and 34 comm...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-12-343
更新日期:2012-12-10 00:00:00
abstract:BACKGROUND:Microscopic examination of peripheral blood smear produces reliable results both about the malaria infection status and level of parasitemia. However, test results are affected by skill of the laboratory personnel, workload, condition of microscopes and quality of laboratory supplies. Therefore, continuous m...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05077-5
更新日期:2020-05-19 00:00:00
abstract:BACKGROUND:Screening household contacts of active tuberculosis (TB) patients is recommended for TB control. Due to resource constraints this rarely occurs in lower income countries. Demographic and clinical features of index cases may influence the likelihood of onwards TB transmission. It has also been proposed that a...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-017-2348-2
更新日期:2017-04-11 00:00:00
abstract:INTRODUCTION:Late presentation to HIV/AIDS care which is attended by problems like, poor treatment outcomes, early development of opportunistic infections, increased healthcare costs, and mortality is a major problem in Ethiopia. Although evidences are available on the prevalence and associated factors of late presenta...
journal_title:BMC infectious diseases
pub_type: 杂志文章,meta分析
doi:10.1186/s12879-019-4156-3
更新日期:2019-06-17 00:00:00