High performance liquid chromatographic determination of glucosamine in rat plasma.

Abstract:

PURPOSE:A high performance liquid chromatographic method was developed for the determination of glucosamine (GlcN) in rat plasma. METHOD:Internal standard, galactosamine, was added to 100 micro L of plasma containing GlcN followed by precipitation of plasma proteins with acetonitrile. Evaporation of the decanted supernatant solution was accelerated by the addition of methanol. GlcN was derivatized by addition of a solution containing 1-naphthyl isothiocyanate. Sample cleanup included passage through an anion exchange cartridge. Analysis was accomplished by injection of 0.1 mL of the sample solution into an isocratic HPLC system consisting of a C18 column, a mobile phase of acetonitrile: water: acetic acid: triethylamine (4.5: 95.5:0.1:0.05), a flow rate of 0.9 mL/min, and a UV detector set at 254 nm. RESULTS:Galactosamine and GlcN appeared 26 and 29 min post-injection, respectively. The assay was linear over the range of 1.25-400 micro g/mL (CV<10%) with a detection limit of 0.63 microg/mL and a limit of quantification of 1.25 microg/mL. The method was applied to the determination of GlcN in rat plasma after oral administration of 350 mg/kg of GlcN hydrochloride. CONCLUSION:The present assay is specific, sensitive, precise, and accurate and is suitable for pharmacokinetic studies.

journal_name

J Pharm Pharm Sci

authors

Aghazadeh-Habashi A,Sattari S,Pasutto F,Jamali F

keywords:

subject

Has Abstract

pub_date

2002-05-01 00:00:00

pages

176-80

issue

2

issn

1482-1826

journal_volume

5

pub_type

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