High performance liquid chromatography of mebudipine: application to pharmacokinetic study.

Abstract:

PURPOSE:To develop a high performance liquid chromatography system for the determination of a new 1,4-dihydropyridine, mebudipine, in rabbit plasma. METHODS:To 1 ml of rabbit plasma was added internal standard (dibudipine) and 0.5 ml of 1 M NaOH. Mebudipine and internal standard were extracted to 5 ml ethyl acetate, evaporated under slow stream of nitrogen. The residue was reconstituted in 200 microl mobile phase and 20 microl of aliquots were injected into a HPLC system equipped with 4.6 x 250 mm i.d. C18 analytical column. Mobile phase consisted of methanol (70%), water (25%) and acetonitril (5%) and its flow rate was 1 ml/min. RESULTS:There were no interfering peaks from endogenous components in blank plasma chromatograms. Standard curves were linear (r(2)>0.99) over 10 to 500 ng/ml. The extraction efficiency was >90% and the minimum quantifiable concentration was 10 ng/ml (CV<10%). CONCLUSION:A suitable, convenient and simple HPLC assay for pharmacokinetic study of mebudipine in rabbits was developed.

journal_name

J Pharm Pharm Sci

authors

Bohlooli S,Keyhanfar F,Mahmoudian M

keywords:

subject

Has Abstract

pub_date

2001-09-01 00:00:00

pages

244-7

issue

3

issn

1482-1826

journal_volume

4

pub_type

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