Abstract:
BACKGROUND:Leishmania donovani is the etiological agent of visceral leishmaniasis (VL) in the Indian subcontinent. However, it is also known to cause cutaneous leishmaniasis (CL) in Sri Lanka. Sri Lankan L. donovani differs from other L. donovani strains, both at the molecular and biochemical level. To investigate the different species or strain-specific differences of L. donovani in Sri Lanka we evaluated sequence variation of the kinetoplastid DNA (kDNA). METHODS:Parasites isolated from skin lesions of 34 CL patients and bone marrow aspirates from 4 VL patients were genotyped using the kDNA minicircle PCR analysis. A total of 301 minicircle sequences that included sequences from Sri Lanka, India, Nepal and six reference species of Leishmania were analyzed. RESULTS:Haplotype diversity of Sri Lankan isolates were high (H d = 0.757) with strong inter-geographical genetic differentiation (F ST > 0.25). In this study, L. donovani isolates clustered according to their geographic origin, while Sri Lankan isolates formed a separate cluster and were clearly distinct from other Leishmania species. Within the Sri Lankan group, there were three distinct sub-clusters formed, from CL patients who responded to standard antimony therapy, CL patients who responded poorly to antimony therapy and from VL patients. There was no specific clustering of sequences based on geographical origin within Sri Lanka. CONCLUSION:This study reveals high levels of haplotype diversity of L. donovani in Sri Lanka with a distinct genetic association with clinically relevant phenotypic characteristics. The use of genetic tools to identify clinically relevant features of Leishmania parasites has important therapeutic implications for leishmaniasis.
journal_name
BMC Infect Disjournal_title
BMC infectious diseasesauthors
Kariyawasam UL,Selvapandiyan A,Rai K,Wani TH,Ahuja K,Beg MA,Premathilake HU,Bhattarai NR,Siriwardena YD,Zhong D,Zhou G,Rijal S,Nakhasi H,Karunaweera NDdoi
10.1186/s12879-017-2883-xsubject
Has Abstractpub_date
2017-12-22 00:00:00pages
791issue
1issn
1471-2334pii
10.1186/s12879-017-2883-xjournal_volume
17pub_type
杂志文章abstract:BACKGROUND:Direct agglutination test (DAT) as a simple, accurate and reliable method, has been widely used for serodiagnosis of visceral leishmaniasis (VL) during the last three decades. The present study is a systematic review and meta-analysis to evaluate the diagnostic accuracy of DAT for serodiagnosis of human VL. ...
journal_title:BMC infectious diseases
pub_type: 杂志文章,meta分析
doi:10.1186/s12879-020-05558-7
更新日期:2020-12-11 00:00:00
abstract:BACKGROUND:Children in developing country suffer the highest burden of pneumonia. However, few studies have evaluated associations between poverty and pneumonia. METHODS:A prospective population-based study on pneumonia was carried out as part of the Latin America Epidemiological Assessment of Pneumococcus (LEAP study...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-11-180
更新日期:2011-06-22 00:00:00
abstract:BACKGROUND:We describe a case of severe Guillain-Barre syndrome (GBS) associated with chronic active hepatitis C and mixed cryoglobulinemia (MC). To our knowledge, this association between GBS and hepatitis C virus (HCV) infection has been rarely reported. CASE PRESENTATION:A 56-year-old man developed symmetrical musc...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4278-7
更新日期:2019-07-17 00:00:00
abstract:BACKGROUND:HIV-infected patients starting antiretroviral treatment (ART) experience deep and early disorders in fat and bone metabolism, leading to concomitant changes in fat mass and bone mineral density. METHODS:We conducted a prospective study in treatment-naive HIV-infected patients randomized to receive two nucle...
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pub_type: 杂志文章,随机对照试验
doi:10.1186/1471-2334-13-293
更新日期:2013-06-28 00:00:00
abstract:BACKGROUND:Worldwide Helicobacter pylori (H.pylori) treatment is of great challenge due to increased antibiotic resistance. The burden of H. pylori antibiotic resistance in Africa is high with unclear information regarding the real magnitude. This systematic review and meta-analysis was conducted to investigate the mag...
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pub_type: 杂志文章,meta分析
doi:10.1186/s12879-018-3099-4
更新日期:2018-04-24 00:00:00
abstract:UNLABELLED:world has not faced a severe pandemic for decades, except the rather mild H1N1 one in 2009, pandemic influenza models are inherently hypothetical and validation is, thus, difficult. We aim at reconstructing a recent seasonal influenza epidemic that occurred in Switzerland and deem this to be a promising vali...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-11-115
更新日期:2011-05-09 00:00:00
abstract:BACKGROUND:Data for predicting which patients with pandemic influenza A (H1N1) infection are likely to run a complicated course are sparse. We retrospectively studied whether the admission serum C-reactive protein (CRP) levels can serve as a predictor of illness severity. METHODS:Included were all consecutive adult pa...
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pub_type: 杂志文章
doi:10.1186/1471-2334-10-288
更新日期:2010-10-04 00:00:00
abstract:BACKGROUND:Polysaccharide pneumococcal vaccine is recommended for use in HIV-infected adults in Brazil but there is uncertainty about its effectiveness in this patient population. The main objective of this study was to assess the effectiveness of the 23-valent polysaccharide pneumococcal vaccine against invasive pneum...
journal_title:BMC infectious diseases
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2334-7-119
更新日期:2007-10-23 00:00:00
abstract:BACKGROUND:The usefulness of procalcitonin (PCT) measurement in critically ill medical patients with suspected nosocomial infection is unclear. The aim of the study was to assess PCT value for the early diagnosis of bacterial nosocomial infection in selected critically ill patients. METHODS:An observational cohort stu...
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pub_type: 杂志文章
doi:10.1186/1471-2334-9-49
更新日期:2009-04-22 00:00:00
abstract:BACKGROUND:The World Health Organization (WHO) recommends viral load (VL) measurement as the preferred monitoring strategy for HIV-infected individuals on antiretroviral therapy (ART) in resource-limited settings. The new WHO guidelines 2016 continue to define virologic failure as two consecutive VL ≥1000 copies/mL (at...
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pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2018-02-12 00:00:00
abstract:BACKGROUND:Diagnosing pneumonia can be challenging in general practice but is essential to distinguish from other respiratory tract infections because of treatment choice and outcome prediction. We determined predictive signs, symptoms and biomarkers for the presence of pneumonia in patients with acute respiratory trac...
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pub_type: 杂志文章
doi:10.1186/s12879-019-4611-1
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abstract:BACKGROUND:High prevalence of risky sexual behaviors and lack of information, skills and preventive support mean that, adolescents face high risks of HIV/AIDS. This study applied the information-motivation-behavioral skills (IMB) model to examine the predictors of consistent condom use among senior high school students...
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pub_type: 杂志文章
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更新日期:2013-06-04 00:00:00
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pub_type: 杂志文章
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abstract:BACKGROUND/AIM:Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common multidrug-resistant organisms in healthcare settings worldwide, but little is known about MRSA transmission outside of acute healthcare settings especially in Asia. We describe the methods for a prospective longitudinal study of...
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pub_type: 杂志文章
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更新日期:2017-10-11 00:00:00
abstract:BACKGROUND:The real burden of smear-positive (PTB+) and bacteriologically confirmed tuberculosis (BCTB) in Ethiopia is not known. Thus, the aim of this community-based study was to measure the prevalence and incidence of tuberculosis in the Hetosa District of Oromia Region, Ethiopia. METHODS:First, a population-based ...
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pub_type: 杂志文章
doi:10.1186/s12879-017-2321-0
更新日期:2017-03-16 00:00:00
abstract:BACKGROUND:Nitric oxide (NO*) plays a pivotal role as a leishmanicidal agent in mouse macrophages. NO* resistant Escherichia coli and Mycobacterium tuberculosis have been associated with a severe outcome of these diseases. METHODS:In this study we evaluated the in vitro toxicity of nitric oxide for the promastigote st...
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pub_type: 杂志文章
doi:10.1186/1471-2334-7-7
更新日期:2007-02-22 00:00:00
abstract:BACKGROUND:Molecular diagnostics have emerged as an efficient and feasible alternative for broad detection of pathogens in faeces. However, collection of stool samples is often impractical in both clinical work and in epidemiology studies. The aim of this study was to investigate the diagnostic performance of rectal sw...
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更新日期:2013-09-27 00:00:00
abstract:BACKGROUND:Identification of people living with HIV is key in HIV prevention and control. Partner Notification service is a World Health Organization backed strategy of reaching out to sexual partners of people diagnosed with HIV for HIV testing. However, its adoption and success rate in Kenya remains unknown. METHODS...
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abstract:BACKGROUND:Community-acquired pneumonia (CAP) remains a major cause of morbidity and mortality throughout the world. Telithromycin (a new ketolide) has shown good in vitro activity against the key causative pathogens of CAP, including S pneumoniae resistant to penicillin and/or macrolides. METHODS:The efficacy and saf...
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pub_type: 临床试验,杂志文章,多中心研究
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abstract:BACKGROUND:Alcohol use has a detrimental impact on the HIV epidemic, especially in sub-Saharan Africa. HIV counseling and testing (HCT) may provide a contact opportunity to intervene with hazardous alcohol use; however, little is known about how alcohol consumption changes following HCT. METHODS:We utilized data from ...
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pub_type: 杂志文章,评审
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更新日期:2019-01-16 00:00:00
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pub_type: 杂志文章,meta分析
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abstract:INTRODUCTION:The World Health Organization recommends that countries conduct two phase evaluations of HIV rapid tests (RTs) in order to come up with the best algorithms. In this report, we present the first ever such evaluation in Uganda, involving both blood and oral based RTs. The role of weak positive (WP) bands on ...
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abstract:BACKGROUND:Antiretroviral therapy (ART) markedly reduces HIV transmission, and testing and treatment programs have been advocated as a method for decreasing transmission at the population level. Little is known, however, about the extent to which sexually transmitted infections (STIs), which increase the HIV infectious...
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abstract:BACKGROUND:American trypanosomiasis is a major disease and public health issue, caused by the protozoan parasite Trypanosoma cruzi. The prevalence of T. cruzi has not been fully documented, and there are few reports of this issue in Nuevo Leon. The aim of this study was to update the seroprevalence rate of T. cruzi inf...
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pub_type: 杂志文章
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