Abstract:
BACKGROUND:Molecular diagnostics have emerged as an efficient and feasible alternative for broad detection of pathogens in faeces. However, collection of stool samples is often impractical in both clinical work and in epidemiology studies. The aim of this study was to investigate the diagnostic performance of rectal swabs as compared with traditional faeces samples for detection of enteric agents by PCR. METHOD:Three hundred twenty-six pairs of rectal swab and stool samples, obtained from Rwandan children aged 0.5-4.99 years, with or without diarrhoea, were analysed by multiple real-time PCR amplifying 3 viral, 6 bacterial and one protozoan target. RESULTS:For all agents there was a significant correlation (R2 0.31-0.85) between Ct values in faeces and rectal swabs. For most agents the Ct values, a marker for target concentration, were significantly lower (by 1-3 cycles) in faeces, indicating pathogen content up to ten times higher than in rectal swabs. Despite this, there was no significant difference in detection rate between faeces and rectal swabs for any agent, reflecting that pathogen concentration was far above the limit of detection in the majority of cases. CONCLUSION:The similar detection rates and the Ct value correlations as compared with traditional faeces samples indicate that rectal swabs are accurate for real-time PCR-based identification of enteric agents and may be used also for quantitative estimation of pathogen load.
journal_name
BMC Infect Disjournal_title
BMC infectious diseasesauthors
Kabayiza JC,Andersson ME,Welinder-Olsson C,Bergström T,Muhirwa G,Lindh Mdoi
10.1186/1471-2334-13-447subject
Has Abstractpub_date
2013-09-27 00:00:00pages
447issn
1471-2334pii
1471-2334-13-447journal_volume
13pub_type
杂志文章abstract:BACKGROUND:Periodontal diseases may affect local and systemic inflammation, and reactive oxygen species (ROS) levels. This systemic health burden could compromise the outcome of pregnancy in expectant mothers. The aim of the present study was to evaluate oxidative stress markers, including glutathione peroxidase (GPx),...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-1003-z
更新日期:2015-07-08 00:00:00
abstract:BACKGROUND:Provision of information plays a critical role in supporting patients to be engaged or empowered to be involved with infection prevention measures in hospitals. This explorative study evaluated the suitability, readability and accessibility of information on healthcare associated infections (HCAIs) and infec...
journal_title:BMC infectious diseases
pub_type: 杂志文章
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journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-0807-1
更新日期:2015-02-22 00:00:00
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pub_type: 杂志文章
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abstract:BACKGROUND:Monitoring the treatment outcomes of tuberculosis and determining the specific factors associated with unsuccessful treatment outcome are essential to evaluate the effectiveness of tuberculosis control program. This study aimed to assess treatment outcomes and explore the factors associated with unsuccessful...
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更新日期:2018-11-29 00:00:00
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journal_title:BMC infectious diseases
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pub_type: 杂志文章,meta分析,评审
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更新日期:2017-02-20 00:00:00
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更新日期:2016-09-27 00:00:00
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pub_type: 杂志文章
doi:10.1186/1471-2334-9-127
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pub_type: 杂志文章,随机对照试验
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pub_type: 杂志文章
doi:10.1186/1471-2334-13-526
更新日期:2013-11-08 00:00:00
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pub_type: 杂志文章
doi:10.1186/1471-2334-8-116
更新日期:2008-09-15 00:00:00
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更新日期:2020-02-26 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12879-016-1963-7
更新日期:2016-11-11 00:00:00
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journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-13-375
更新日期:2013-08-15 00:00:00
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pub_type: 杂志文章
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journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-10-260
更新日期:2010-09-03 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12879-017-2302-3
更新日期:2017-03-14 00:00:00