Abstract:
BACKGROUND:Upregulation of Cyclin dependent kinase 1 (CDK1) protein is closely related with the prognosis of several malignant tumors. Chk1-CDC25C-CDK1 signaling and P53-P21WAF1-CDK1 signaling pathways are closely related with the cell cycle G2/M phase regulation. The present study aimed to analyze the relationship between CDK1 and the proliferation and apoptosis of ovarian cancer cells, investigate its molecular mechanism preliminarily. METHODS:The specific short-hair RNA (shRNA) plasmids and negative control plasmid of CDK1, checkpoint kinase 1 (CHK1) and p53 genes were transfected into ovarian cancer SK-OV-3 and OVCAR-3 cells respectively. The expressions of CDK1, CHK1 and p53 mRNA and CDK1, Chk1 and P53 protein were detected by sqRT-PCR and Western blot, levels of phospho-CDK1(Thr14/Tyr15), CyclinB1, phospho-Chk1(ser345), cell division cycle 25C (CDC25C), phospho-CDC25C(ser216), P21WAF1, phospho-P53(ser15), proliferating cell nuclear antigen (PCNA), Ki-67, Bcl-2, Bax, Caspase8, Cleaved-caspase3 and Cytochrome C were examined by Western blot. The cell proliferation was measured by MTT and Trypan blue exclusion assay respectively, the cell cycle phase distribution and cell apoptosis rate were detected by flow cytometry (FCM) assay. RESULTS:As results of CDK1 inhibition by shRNA, the cell proliferation was repressed, the cell numbers of G2/M phase and cell apoptosis rate were increased in both SK-OV-3 and OVCAR-3 cells. After knockdown of CDK1, expressions of PCNA, Ki-67 and Bcl-2 protein were downregulated, expressions of Bax, Caspase8, Cleaved-caspase3 and Cytochrome C were upregulated. While knockdown the CHK1 and p53 by shRNA respectively, the similar effects were observed on the cell proliferation, cell cycle phase distribution and apoptosis in both SK-OV-3 and OVCAR-3 cells, as well as the expressions of the proliferation and apoptosis related proteins mentioned above. Moreover, the levels of p-CDK1(Thr14/Tyr15) were increased after either CHK1 inhibition or p53 inhibition. CONCLUSIONS:Abnormal activation of CDK1 was implicated in the proliferation and apoptosis regulation of ovarian cancer cells, which might be due to the aberrant regulations of the upstream Chk1-CDC25C and P53-P21WAF1 signaling pathway.
journal_name
J Ovarian Resjournal_title
Journal of ovarian researchauthors
Zhang R,Shi H,Ren F,Zhang M,Ji P,Wang W,Liu Cdoi
10.1186/s13048-017-0356-xsubject
Has Abstractpub_date
2017-09-12 00:00:00pages
60issue
1issn
1757-2215pii
10.1186/s13048-017-0356-xjournal_volume
10pub_type
杂志文章abstract:BACKGROUND:This study aimed to identify the clinical and pathological characteristics and the possible prognostic factors for Chinese patients with early-stage ovarian endometrioid carcinoma. METHODS:The present study reviewed the medical records of patients who received initial treatment and a postoperative pathologi...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-017-0361-0
更新日期:2017-09-18 00:00:00
abstract:INTRODUCTION:Weight gain and fat accumulation are predisposing factors of PCOS. Life-style modification, including increasing physical activity, is the first line approach in managing PCOS. The objective of this study is to assess the effect of exercise intensity on weight changes, androstenedione and free testosterone...
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abstract:BACKGROUND:This study aimed to examine the performance of the four risk of malignancy index (RMI) in discriminating borderline ovarian tumors (BOTs) and benign ovarian masses in daily clinical practice. METHODS:A total of 162 women with BOTs and 379 women with benign ovarian tumors diagnosed at the Second Affiliated H...
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pub_type: 杂志文章
doi:10.1186/s13048-019-0568-3
更新日期:2019-09-16 00:00:00
abstract::RNA methylation can reverse the methylation modification at the RNA level, which is an extremely important epigenetic modification. The function and mechanism of YTHDF2, as a reader of m6A modification, in epithelial ovarian cancer (EOC) have not been elucidated so far. This study aimed to investigate how YTHDF2 and m...
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pub_type: 杂志文章
doi:10.1186/s13048-020-00717-5
更新日期:2020-09-18 00:00:00
abstract:BACKGROUND:The aim of this study was to evaluate the concentrations of IgG antibodies against Hsp60 and Hsp65 in sera of patients with ovarian cancer at various stages of clinical progress and for different histopathological types of disease. METHODS:Serum samples from 149 patients with ovarian carcinoma and 80 health...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-7-30
更新日期:2014-03-11 00:00:00
abstract:BACKGROUND:Because higher survival of follicles during the freezing/thawing procedure improves the quality of cryopreserved tissue reimplanted after oncological therapies, defining an optimal method for human ovarian tissue cryopreservation remains a major issue in this field. One option to improve the cryopreservation...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-016-0268-1
更新日期:2016-09-26 00:00:00
abstract::We have earlier reported the presence of very small embryonic-like stem cells (VSELs) in adult mouse uterus along with slightly bigger progenitors termed endometrial stem cells (EnSCs) and their regulation by ovarian hormones thus demonstrating a crucial role played by them during proliferation, differentiation and re...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-017-0324-5
更新日期:2017-04-24 00:00:00
abstract:BACKGROUND:The mechanisms regulating the function and regression of the corpus luteum (CL) have not yet been elucidated in detail. The regressed CL of cows was previously reported to be filled with unusual vessels like arteriovenous anastomosis (AVA); however how these vessels are being established during luteolysis re...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-016-0277-0
更新日期:2016-10-19 00:00:00
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journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-020-00624-9
更新日期:2020-03-05 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-015-0203-x
更新日期:2015-11-14 00:00:00
abstract:BACKGROUND:Ovarian cancer is the second most common gynecologic malignancy. As the primary imaging modality, computed tomography (CT) can provide staging information for preoperative planning and determination of surgical resectability. As a new three-dimensional postprocessing tool for CT images, cinematic rendering (...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-018-0461-5
更新日期:2018-09-26 00:00:00
abstract:UNLABELLED: BACKGROUND:To investigate the 3.0-Tesla (3 T) magnetic resonance imaging (MRI) characteristics of primary adnexal lesions for discriminating benign from malignant lesions. METHODS:One hundred thirty-nine patients with pathologically proven primary adnexal masses referred for 3 T MRI assessment preoperativ...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-5-33
更新日期:2012-11-14 00:00:00
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journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-016-0238-7
更新日期:2016-05-21 00:00:00
abstract::An amendment to this paper has been published and can be accessed via the original article. ...
journal_title:Journal of ovarian research
pub_type: 已发布勘误
doi:10.1186/s13048-020-00733-5
更新日期:2020-11-12 00:00:00
abstract:BACKGROUND:Despite advances in surgical aggressiveness and conventional chemotherapy, ovarian cancer remains the most lethal cause of gynecologic cancer mortality; consequently there is a need for new therapeutic agents and innovative treatment paradigms for the treatment of ovarian cancer. Several studies have demonst...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-016-0282-3
更新日期:2016-10-27 00:00:00
abstract:BACKGROUND:Epithelial ovarian cancer (EOC) is still a major gynecologic problem with poor 5 year survival rate due to distance metastases, despite routine surgery and chemotherapy. The precise underlying molecular mechanisms that trigger EOC migration and invasion are unclear. Recent studies suggest that the expression...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-6-84
更新日期:2013-11-26 00:00:00
abstract::Ovarian hyperstimulation syndrome (OHSS) is a serious complication of controlled ovarian hyperstimulation (COH). The syndrome almost always presents either after hCG administration in susceptible patients or during early pregnancy. Despite many years of clinical experience, there are no precise methods to completely p...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-6-77
更新日期:2013-11-05 00:00:00
abstract:RATIONALE AND OBJECTIVES:To evaluate the impact of metabolic parameters in the peritoneal cavity on the likelihood of achieving complete tumor debulking in patients with ovarian and peritoneal cancers. MATERIALS AND METHODS:Forty-nine patients with ovarian and peritoneal cancers were included, who underwent pre-operat...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-020-00662-3
更新日期:2020-05-20 00:00:00
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journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-018-0433-9
更新日期:2018-07-24 00:00:00
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journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-019-0561-x
更新日期:2019-09-23 00:00:00
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journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-017-0362-z
更新日期:2017-09-26 00:00:00
abstract::Currently, the world is in the seventh month of the COVID-19 pandemic. Globally, infections with novel SARS-CoV-2 virus are continuously rising with mounting numbers of deaths. International and local public health responses, almost in synchrony, imposed restrictions to minimize spread of the virus, overload of health...
journal_title:Journal of ovarian research
pub_type: 杂志文章,评审
doi:10.1186/s13048-020-00737-1
更新日期:2020-11-27 00:00:00
abstract:INTRODUCTION:The use of patient reported outcome measures (PROMs) has increased during the past decade, and the focus on how to use them has resulted in a more proactive application. Studies have shown that proactive use of PROMs during treatment improves patient-clinician communication, leads to better symptom managem...
journal_title:Journal of ovarian research
pub_type: 杂志文章,评审
doi:10.1186/s13048-019-0538-9
更新日期:2019-07-15 00:00:00
abstract:BACKGROUND:galectin-1 has been implicated in tumor invasion and metastasis and is frequently over-expressed in epithelial ovarian cancer (EOC), but its potential as a biomarker remains unclear. In this novel study, we have explored the possible use of galectin-1 as a biomarker for EOC. METHODS:galectin-1 in sera was e...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-015-0206-7
更新日期:2015-11-21 00:00:00
abstract:BACKGROUND:Mucinous carcinomas from different organs are morphologically similar and might share similarities at the molecular and biochemical levels that may illuminate their pathogenesis and influence management. The factors involved in the pathogenesis of mucinous carcinomas remain unknown; which is likely one contr...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-015-0169-8
更新日期:2015-06-17 00:00:00
abstract:BACKGROUND:The imbalance of expression of microRNA-802 may have a significant place in tumor progression. However, the bio-function of epithelial ovarian cancer cells remains unclear. Therefore, we setup this study to explore the pathogenesis of epithelial ovarian cancer based on microRNA-802. METHODS:RT-qPCR analysis...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-019-0576-3
更新日期:2019-10-22 00:00:00
abstract:BACKGROUND:Altered androgen hormone homeostasis and androgen receptor (AR) activity have been implicated in ovarian carcinogenesis but the relationship between AR expression in ovarian cancer and clinical outcome remains unclear. METHODS:In this study, the prognostic impact of AR expression was investigated using immu...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-3-14
更新日期:2010-06-17 00:00:00
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journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-4-20
更新日期:2011-11-10 00:00:00
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journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-7-50
更新日期:2014-05-07 00:00:00