Abstract:
BACKGROUND:Ambiguity in malignant transformation of glioma has made prognostic diagnosis very challenging. Tumor malignant transformation is closely correlated with specific alterations of the metabolic profile. Exploration of the underlying metabolic alterations in glioma cells of different malignant degree is therefore vital to develop metabolic biomarkers for prognosis monitoring. METHODS:We conducted (1)H nuclear magnetic resonance (NMR)-based metabolic analysis on cell lines (CHG5, SHG44, U87, U118, U251) developed from gliomas of different malignant grades (WHO II and WHO IV). Several methods were applied to analyze the (1)H-NMR spectral data of polar extracts of cell lines and to identify characteristic metabolites, including principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), fuzzy c-means clustering (FCM) analysis and orthogonal projection to latent structure with discriminant analysis (OPLS-DA). The expression analyses of glial fibrillary acidic protein (GFAP) and matrix metal proteinases (MMP-9) were used to assess malignant behaviors of cell lines. GeneGo pathway analysis was used to associate characteristic metabolites with malignant behavior protein markers GFAP and MMP-9. RESULTS:Stable and distinct metabolic profiles of the five cell lines were obtained. The metabolic profiles of the low malignancy grade group (CHG5, SHG44) were clearly distinguished from those of the high malignancy grade group (U87, U118, U251). Seventeen characteristic metabolites were identified that could distinguish the metabolic profiles of the two groups, nine of which were mapped to processes related to GFAP and MMP-9. Furthermore, the results from both quantitative comparison and metabolic correlation analysis indicated that the significantly altered metabolites were primarily involved in perturbation of metabolic pathways of tricarboxylic acid (TCA) cycle anaplerotic flux, amino acid metabolism, anti-oxidant mechanism and choline metabolism, which could be correlated with the changes in the glioma cells' malignant behaviors. CONCLUSIONS:Our results reveal the metabolic heterogeneity of glioma cell lines with different degrees of malignancy. The obtained metabolic profiles and characteristic metabolites are closely associated with the malignant features of glioma cells, which may lay the basis for both determining the molecular mechanisms underlying glioma malignant transformation and exploiting non-invasive biomarkers for prognosis monitoring.
journal_name
Mol Cancerjournal_title
Molecular cancerauthors
Shao W,Gu J,Huang C,Liu D,Huang H,Huang Z,Lin Z,Yang W,Liu K,Lin D,Ji Tdoi
10.1186/1476-4598-13-197subject
Has Abstractpub_date
2014-08-27 00:00:00pages
197issn
1476-4598pii
1476-4598-13-197journal_volume
13pub_type
杂志文章相关文献
Molecular Cancer文献大全abstract:BACKGROUND:Aurora-A is an oncogenic kinase playing well-documented roles in mitotic spindle organisation. We previously found that Aurora-A inactivation yields the formation of spindles with fragmented poles that can drive chromosome mis-segregation. Here we have addressed the mechanism through which Aurora-A activity ...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-10-131
更新日期:2011-10-19 00:00:00
abstract:BACKGROUND:Tumor recurrence and metastasis develop as a result of tumors' acquisition of anti-apoptotic mechanisms and therefore, it is necessary to develop novel effective therapeutics against metastatic cancers. In this study, we showed the differential TRAIL responsiveness of human prostate adenocarcinoma PC3 and hu...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-10-46
更新日期:2011-04-25 00:00:00
abstract:BACKGROUND:CD44 is considered as 'a' metastasis associated gene, despite the fact that it is an umbrella term for a group of molecules produced from a single gene by alternative splicing. However, little consideration is given to the above in the literature of colorectal carcinomas as well as other tumour types, leadin...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-11-83
更新日期:2012-11-14 00:00:00
abstract::Sema4D, also known as CD100, is a protein belonging to class IV semaphorin. Its physiologic roles in the immune and nervous systems have been extensively explored. However, the roles of Sema4D have extended beyond these traditionally studied territories. Via interaction with its high affinity receptor Plexin-B1, Sema4...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/1476-4598-9-251
更新日期:2010-09-21 00:00:00
abstract:PURPOSE:Hypoxia is a major regulator of angiogenesis and always influences the release of exosomes in various types of tumors. The present review aimed to assess the role of hypoxia-induced exosomes in the tumor biology. METHODS:The relevant publications were retrieved from PubMed using keywords such as hypoxia, exoso...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/s12943-018-0869-y
更新日期:2018-08-11 00:00:00
abstract::Although solid tumors comprise malignant cells, they also contain many different non-malignant cell types in their micro-environment. The cellular components of the tumor stroma consist of immune and endothelial cells combined with a heterogeneous population of stromal cells which include cancer-associated fibroblasts...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/s12943-019-0960-z
更新日期:2019-03-30 00:00:00
abstract:BACKGROUND:New therapies are urgently needed for patients with small cell lung cancer (SCLC). Chemotherapy and targeted therapies, including the Bcl-2 inhibitor ABT-737, may induce tumor cell autophagy. Autophagy can promote survival of cancer cells under stress and comprise a pathway of escape from cytotoxic therapies...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-12-16
更新日期:2013-03-02 00:00:00
abstract:BACKGROUND:The role of TNF-alpha in affecting the fate of tumors is controversial, while some studies have reported apoptotic or necrotic effects of TNF-alpha, others provide evidence that endogenous TNF-alpha promotes growth and development of tumors. Understanding the mechanism(s) of TNF-alpha mediated growth arrest ...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-6-42
更新日期:2007-06-12 00:00:00
abstract:BACKGROUND:CADM1 encodes an immunoglobulin superfamily (IGSF) cell adhesion molecule. Inactivation of CADM1, either by promoter hypermethylation or loss of heterozygosity, has been reported in a wide variety of tumor types, thus it has been postulated as a tumor suppressor gene. FINDINGS:We show for the first time tha...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-11-29
更新日期:2012-05-03 00:00:00
abstract:BACKGROUND:High expression of the long non-coding RNA nuclear-enriched abundant transcript 1 (NEAT1) in whole blood has been reported in colorectal cancer patients; however, its' clinical significance and origin are unclear. We evaluated the diagnostic and prognostic value, and origin of whole blood NEAT1 in colorectal...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-015-0455-5
更新日期:2015-11-09 00:00:00
abstract::Cancer is a leading cause of death worldwide, particularly because of its high mortality rate in patients who are diagnosed at late stages. Conventional biomarkers originating from blood are widely used for cancer diagnosis, but their low sensitivity and specificity limit their widespread application in cancer screeni...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/s12943-020-01298-z
更新日期:2021-01-11 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one of the most common malignant tumours with a poor prognosis worldwide. While early stage tumours can be treated with curative approaches such as liver transplantation or surgical resection, these are only suitable for a minority of patients. Those with advanced stage disease are on...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/s12943-017-0734-4
更新日期:2017-10-23 00:00:00
abstract:BACKGROUND:miR-139-5p was identified to be significantly down-regulated in colon tumor tissues by miRNA array. We aimed to clarify its biological function, molecular mechanisms and direct target gene in colorectal cancer (CRC). METHODS:The biological function of miR-139-5p was examined by cell growth, cell cycle and a...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-13-124
更新日期:2014-05-26 00:00:00
abstract:BACKGROUND:The human microbial pathogen Helicobacter pylori resides in the stomach of about fifty percent of the world's population and represents a risk factor for chronic gastritis, peptic ulcers and, in rare cases, gastric cancer. Alterations of the Wnt/beta-catenin signaling pathway have been described in almost ev...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-9-31
更新日期:2010-02-05 00:00:00
abstract:BACKGROUND:Differential mRNA splicing and alternative promoter usage of the TP73 gene results in the expression of multiple NH2-truncated isoforms that act as oncogenes. Abundant levels of these p73 variants in a variety of human cancers correlated with adverse clinical prognosis and response failure to conventional th...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-8-61
更新日期:2009-08-11 00:00:00
abstract:BACKGROUND:For long, natural killer (NK) cells have been suspected to play a critical role in suppressing the development of spontaneous metastases in cancer patients. Despite a wide range of studies it remains unclear so far to what extent primary tumor growth together with formation of distant metastases and NK cell ...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-13-244
更新日期:2014-11-05 00:00:00
abstract:INTRODUCTION:Tumor-initiating cells (TICs), aka "cancer stem cells", are believed to fuel tumors and to sustain therapy resistance and systemic metastasis. Breast cancer is the first human carcinoma in which a subpopulation of cells displaying a specific CD44+/CD24-/low/ESA+ antigenic phenotype was found to have TIC pr...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-13-213
更新日期:2014-09-12 00:00:00
abstract:BACKGROUND:The implementation of gene therapy for the treatment of pituitary tumors emerges as a promising complement to surgery and may have distinct advantages over radiotherapy for this type of tumors. Up to now, suicide gene therapy has been the main experimental approach explored to treat experimental pituitary tu...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-7-13
更新日期:2008-01-25 00:00:00
abstract:BACKGROUND:In previously published studies, oncolytic adenovirus-mediated gene therapy has produced good results in targeting cancer cells. However, safety and efficacy, the two most important aspects in cancer therapy, remain serious challenges. The specific expression or deletion of replication related genes in an ad...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-10-133
更新日期:2011-11-01 00:00:00
abstract:BACKGROUND:Osteopontin (OPN) has been shown to play many roles in the progression of cancer. We have recently demonstrated the activation of Akt by OPN. Integrin-linked kinase and PI3-kinase are integral proteins in OPN/AKT pathway in PC3 cells. To investigate the role of the extracellular receptors in OPN signaling, w...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-9-260
更新日期:2010-09-26 00:00:00
abstract:BACKGROUND:In contrast to tumor-suppressive apoptosis and autophagic cell death, necrosis promotes tumor progression by releasing the pro-inflammatory and tumor-promoting cytokine high mobility group box 1 (HMGB1), and its presence in tumor patients is associated with poor prognosis. Thus, necrosis has important clinic...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-10-113
更新日期:2011-09-14 00:00:00
abstract:BACKGROUND:Taurine upregulated gene1 (TUG1) as a 7.1-kb lncRNA, has been shown to play an oncogenic role in various cancers. However, the biological functions of lncRNA TUG1 in small cell lung cancer (SCLC) remain unknown. The aim of this study is to explore the roles of TUG1 in cell growth and chemoresistance of SCLC ...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-016-0575-6
更新日期:2017-01-09 00:00:00
abstract::The mammalian target of rapamycin (mTOR), which exists in two functionally distinct complexes, mTORC1 and mTORC2 plays an important role in tumor growth. Whereas the role of mTORC1 has been well characterized in this process, little is known about the functions of mTORC2 in cancer progression. In this study, we explor...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-9-57
更新日期:2010-03-12 00:00:00
abstract:BACKGROUND:Colorectal cancers carrying the B-Raf V600E-mutation are associated with a poor prognosis. The purpose of this study was to identify B-RafV600E-mediated traits of cancer cells in a genetic in vitro model and to assess the selective sensitization of B-RafV600E-mutant cancer cells towards therapeutic agents. ...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-13-122
更新日期:2014-05-24 00:00:00
abstract:BACKGROUND:Abnormal amplification/expression of HER-2/neu oncogene has been causally linked with tumorigenesis and metastasis in breast cancer and associated with shortened overall survival of patients. Recently, heat shock protein 27 (Hsp27) was reported to be highly expressed in HER-2/neu positive tumors and cell lin...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-6-52
更新日期:2007-08-14 00:00:00
abstract:BACKGROUND:MicroRNAs (miRNAs) are involved in cancer development and progression, acting as tumor suppressors or oncogenes. Our previous studies have revealed that miR-148a and miR-152 are significantly down-regulated in gastrointestinal cancers. Interestingly, miR-148b has the same "seed sequences" as miR-148a and miR...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-10-1
更新日期:2011-01-04 00:00:00
abstract:BACKGROUND:The human cell cycle transcription factor FOXM1 is known to play a key role in regulating timely mitotic progression and accurate chromosomal segregation during cell division. Deregulation of FOXM1 has been linked to a majority of human cancers. We previously showed that FOXM1 was upregulated in basal cell c...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-9-45
更新日期:2010-02-26 00:00:00
abstract::At the moment, pancreatic cancer is among the deadliest gastrointestinal diseases, and pancreatic cancer growth is a complex biological process that is based on different kinds of genes. Exosomes are extracellular vesicles containing microRNAs (miRNAs), messenger RNA (mRNA), and proteins, they act as the most prominen...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/s12943-020-01245-y
更新日期:2020-08-27 00:00:00
abstract:BACKGROUND:Survivin is a dual function protein. It inhibits the apoptosis of cells by inhibiting caspases, and also promotes cell growth by stabilizing microtubules during mitosis. Over-expression of survivin has been demonstrated to induce drug-resistance to various chemo-therapeutic agents such as cisplatin (DNA dama...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-8-43
更新日期:2009-07-03 00:00:00
abstract::The correlation between epigenetic aberrations and disease underscores the importance of epigenetic mechanisms. Here, we review recent findings regarding chromatin modifications and their relevance to cancer. ...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/1476-4598-5-60
更新日期:2006-11-08 00:00:00