Abstract：
BACKGROUND:Our recent study on a panel of human ovarian cancer cells revealed that SKOV-3 cells barely express the Sprouty isoform 1 (Spry1) while 1A9 cells maintain it at a level similar to normal ovarian cells. Here we investigated the functional outcomes of induced alterations in the expression of Spry1 in the two cell lines in vitro. METHODS:Using the Spry1 specific plasmid and siRNA, the expression of Spry1 was induced and conversely silenced in SKOV-3 and 1A9 cells, respectively. The functional outcome was investigated by means of proliferation, MTT, scratch-wound, migration and invasion assays and selection of the stable clones. Mechanism of the effect was explored by Western blot. RESULTS:In the Spry1-transfected SKOV-3 cells, a significant reduction in growth and proliferation was evident. Stable clones of the Spry1-transfected SKOV-3 were almost undetectable after day 14. The number of migrated and invaded cells and the percentage of the scratch closure were significantly lower in the Spry1-transfected group. Spry1 silencing in 1A9 cells, on the other hand, led to a significant increase in cell growth and proliferation. The number of migrated and invaded cells and the percentage of the scratch closure significantly increased in Spry1-silenced 1A9 group. Mechanistically, overexpression of Bax, activation of caspases 3, 7, 8 and 9, cleavage of PARP and attenuation of Bcl-2 and Bcl-xl were observed along with reduced activation of Erk and Akt and increased amount and activity of PTEN in the Spry1-transfected SKOV-3 cells. CONCLUSIONS:Here, we report the inverse correlation between the expression of Spry1 and growth, proliferation, invasion and migration of ovarian cancer cells.
journal_name
J Ovarian Resjournal_title
Journal of ovarian researchauthors
Masoumi-Moghaddam S,Amini A,Ehteda A,Wei AQ,Morris DLdoi
10.1186/1757-2215-7-61subject
Has Abstractpub_date
2014-06-08 00:00:00pages
61issn
1757-2215pii
1757-2215-7-61journal_volume
7pub_type
杂志文章abstract：BACKGROUND:The imbalance of expression of microRNA-802 may have a significant place in tumor progression. However, the bio-function of epithelial ovarian cancer cells remains unclear. Therefore, we setup this study to explore the pathogenesis of epithelial ovarian cancer based on microRNA-802. METHODS:RT-qPCR analysis...
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journal_title：Journal of ovarian research
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doi：10.1186/s13048-018-0398-8
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pub_type： 杂志文章
doi：10.1186/s13048-018-0461-5
更新日期：2018-09-26 00:00:00
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journal_title：Journal of ovarian research
pub_type： 杂志文章
doi：10.1186/1757-2215-2-8
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abstract：INTRODUCTION:Weight gain and fat accumulation are predisposing factors of PCOS. Life-style modification, including increasing physical activity, is the first line approach in managing PCOS. The objective of this study is to assess the effect of exercise intensity on weight changes, androstenedione and free testosterone...
journal_title：Journal of ovarian research
pub_type： 杂志文章
doi：10.1186/1757-2215-7-37
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pub_type： 杂志文章
doi：10.1186/1757-2215-6-36
更新日期：2013-05-15 00:00:00
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pub_type： 杂志文章,已发布勘误
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更新日期：2020-03-13 00:00:00
abstract：BACKGROUND:Altered androgen hormone homeostasis and androgen receptor (AR) activity have been implicated in ovarian carcinogenesis but the relationship between AR expression in ovarian cancer and clinical outcome remains unclear. METHODS:In this study, the prognostic impact of AR expression was investigated using immu...
journal_title：Journal of ovarian research
pub_type： 杂志文章
doi：10.1186/1757-2215-3-14
更新日期：2010-06-17 00:00:00
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doi：10.1186/s13048-019-0506-4
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journal_title：Journal of ovarian research
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journal_title：Journal of ovarian research
pub_type： 杂志文章,评审
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更新日期：2019-07-15 00:00:00
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journal_title：Journal of ovarian research
pub_type： 杂志文章
doi：10.1186/s13048-018-0476-y
更新日期：2019-01-10 00:00:00
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journal_title：Journal of ovarian research
pub_type： 杂志文章
doi：10.1186/s13048-019-0592-3
更新日期：2019-11-18 00:00:00
abstract：UNLABELLED: BACKGROUND:Women with polycystic ovary syndrome (PCOS) have higher risk for cardiovascular disease (CVD). Heart type fatty acid binding protein (HFABP) has been found to be predictive for myocardial ischemia.Wet ested whether HFABP is the predictor for CVD in PCOS patients, who have an increased risk of ca...
journal_title：Journal of ovarian research
pub_type： 杂志文章
doi：10.1186/1757-2215-5-45
更新日期：2012-12-18 00:00:00
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journal_title：Journal of ovarian research
pub_type： 杂志文章
doi：10.1186/s13048-016-0291-2
更新日期：2016-11-22 00:00:00
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journal_title：Journal of ovarian research
pub_type： 杂志文章
doi：10.1186/1757-2215-7-10
更新日期：2014-01-21 00:00:00
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journal_title：Journal of ovarian research
pub_type： 杂志文章
doi：10.1186/s13048-016-0265-4
更新日期：2016-09-07 00:00:00
abstract：:The diagnosis of primary angiosarcoma of ovary is still a challenge as it has no specific clinical symptoms and is easily confused with other malignant neoplasms in morphology. Here, we described a case of primary ovarian angiosarcoma and reviewed the literature. A 47-year-old female showed a left ovary mass. Grossly,...
journal_title：Journal of ovarian research
pub_type： 杂志文章
doi：10.1186/s13048-021-00771-7
更新日期：2021-01-28 00:00:00
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journal_title：Journal of ovarian research
pub_type： 杂志文章,评审
doi：10.1186/s13048-020-00737-1
更新日期：2020-11-27 00:00:00
abstract：BACKGROUND:It is estimated that more than 20% of ovarian cancer cases are associated with a genetic predisposition that is only partially explained by germline mutations in the BRCA1 and BRCA2 genes. Recently, several pieces of evidence showed that mutations in three genes involved in the homologous recombination DNA r...
journal_title：Journal of ovarian research
pub_type： 杂志文章,meta分析
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更新日期：2020-05-02 00:00:00
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journal_title：Journal of ovarian research
pub_type： 杂志文章
doi：10.1186/s13048-016-0268-1
更新日期：2016-09-26 00:00:00
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pub_type： 杂志文章
doi：10.1186/s13048-017-0362-z
更新日期：2017-09-26 00:00:00
abstract：BACKGROUND:Recent researches demonstrate that pre-treatment with androgen could increase retrieved oocytes number and clinical pregnancy rate in poor ovarian response (POR) patients. In view of gap junction intercellular communication (GJIC) is important for follicular growth, and androgen plays an important role in im...
journal_title：Journal of ovarian research
pub_type： 杂志文章
doi：10.1186/s13048-016-0290-3
更新日期：2016-11-23 00:00:00
abstract：BACKGROUND:Polycystic Ovary Syndrome (PCOS) is a widespread reproductive disorder characterized by a disruption of follicular growth and anovulatory infertility. In women with PCOS, follicular growth and ovulation can be induced by subcutaneous injections of low doses of follicle stimulating hormone (FSH). The aim of t...
journal_title：Journal of ovarian research
pub_type： 杂志文章
doi：10.1186/s13048-015-0192-9
更新日期：2015-10-06 00:00:00
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journal_title：Journal of ovarian research
pub_type： 杂志文章
doi：10.1186/s13048-018-0404-1
更新日期：2018-04-10 00:00:00
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journal_title：Journal of ovarian research
pub_type： 杂志文章,评审
doi：10.1186/s13048-015-0131-9
更新日期：2015-02-25 00:00:00
abstract：BACKGROUND:Metformin decreases polycystic ovary syndrome (PCOS) symptoms, induces ovulation, and may improve developmental competence of in vitro oocyte maturation. This study was designed to define the effects of metformin on the characteristics of in vitro oocyte maturation in estradiol valerate (EV) PCOS-induced rat...
journal_title：Journal of ovarian research
pub_type： 杂志文章
doi：10.1186/s13048-015-0203-x
更新日期：2015-11-14 00:00:00
abstract：:Following publication of the original article [1], the authors opted to correct the incorrect e-mail address of the corresponding author from 82-55-360-1850chungsu1982@gmail.com to chungsu1982@gmail.com , grant in the Acknowledgements section and Author details. Below are the updated version of the Acknowledgements an...
journal_title：Journal of ovarian research
pub_type： 已发布勘误
doi：10.1186/s13048-019-0540-2
更新日期：2019-07-23 00:00:00
abstract：:We have earlier reported the presence of very small embryonic-like stem cells (VSELs) in adult mouse uterus along with slightly bigger progenitors termed endometrial stem cells (EnSCs) and their regulation by ovarian hormones thus demonstrating a crucial role played by them during proliferation, differentiation and re...
journal_title：Journal of ovarian research
pub_type： 杂志文章
doi：10.1186/s13048-017-0324-5
更新日期：2017-04-24 00:00:00
