Abstract:
:An improved methodology is presented here for transgenic Plasmodium berghei lines that express the negative selectable marker yFCU (a bifunctional protein that combines yeast cytosine deaminase and uridyl phosphoribosyl transferase (UPRT)) and substitutes delivery of selection drug 5-fluorocytosine (5FC) by intraperitoneal injection for administration via the drinking water of the mice. The improved methodology is shown to be as effective, less labour-intensive, reduces animal handling and animal numbers required for successful selection thereby contributing to two of the "three Rs" of animal experimentation, namely refinement and reduction.
journal_name
Malar Jjournal_title
Malaria journalauthors
Orr RY,Philip N,Waters APdoi
10.1186/1475-2875-11-103subject
Has Abstractpub_date
2012-03-31 00:00:00pages
103issn
1475-2875pii
1475-2875-11-103journal_volume
11pub_type
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