Abstract:
PURPOSE:The experiments were designed to provide details on the characteristics of rhodopsin regeneration in the rod photoreceptors of diabetic mice and to evaluate their mechanistic basis. METHODS:A genetically-derived diabetic albino mouse was developed. An excised albino mouse eye preparation was used. The preparation allows for direct measurements of rhodopsin concentration and other spectrally distinct intermediates, and maintains the structural integrity of the compartments involved in rhodopsin regeneration. The experiments were conducted at moderate bleaching levels, averaging 13-14%, with mice that exhibited moderate levels of blood glucose concentration. RESULTS:Rhodopsin regeneration was delayed in both genetically-derived and streptozotocin-injected diabetic mice when compared to non-diabetic mice. During the delay an intermediate of rhodopsin, N-Retinylidene Opsin (NRO440), that precedes the hydrolysis of the bleached chromophore from the opsin and that is associated with acidic conditions was observed. This intermediate was not observed in non-diabetic mice. The delay in rhodopsin regeneration of the diabetic mice was partially eliminated, and the relative concentration of NRO440 was decreased, when the internal pH of the rod photoreceptor was raised by modifying the perfusate composition. CONCLUSIONS:Following a bleach, both genetic and streptozotocin diabetic mice exhibited abnormalities in rhodopsin regeneration. When studied at moderate bleaching levels with animals that were moderately diabetic, both groups of diabetic mice exhibited a delay in the start of the rhodopsin regeneration. The delay appears to be caused by the formation of an acid intermediate of the bleached rhodopsin resulting from acid conditions within the rod photoreceptors.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Ostroy SEdoi
10.1076/ceyr.17.10.979.5236subject
Has Abstractpub_date
1998-10-01 00:00:00pages
979-85issue
10eissn
0271-3683issn
1460-2202journal_volume
17pub_type
杂志文章abstract:AIM OF THE STUDY:The similarity of many benign intrabulbar lesions to a malignant tumor requires detailed differential diagnostics. However, none of the known methods can be used as the only one to determine the type of lesions. The aim of this study was to determine color Doppler imaging (CDI) markers characteristic o...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1341534
更新日期:2017-11-01 00:00:00
abstract::Change of urate and ascorbate levels in retinae of rat left eyes after ophthalmic artery and optic nerve ligation was studied by reversed-phase high-performance liquid chromatography with electrochemical detection. The right eyes were used for sham operation. We found that uric acid levels in the retinae of the left e...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688608996369
更新日期:1986-07-01 00:00:00
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更新日期:2020-09-11 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609017634
更新日期:1996-09-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.24.1.12.5426
更新日期:2002-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688909025807
更新日期:1989-07-01 00:00:00
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更新日期:2004-08-01 00:00:00
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pub_type: 杂志文章
doi:10.3109/02713683.2016.1167920
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journal_title:Current eye research
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journal_title:Current eye research
pub_type: 杂志文章
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journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
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更新日期:1992-12-01 00:00:00
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doi:10.3109/02713688509003352
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pub_type: 杂志文章
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更新日期:2020-07-01 00:00:00
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更新日期:1988-03-01 00:00:00
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更新日期:2002-03-01 00:00:00
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更新日期:2008-04-01 00:00:00
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更新日期:2012-09-01 00:00:00
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更新日期:1997-02-01 00:00:00
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更新日期:2009-10-01 00:00:00
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更新日期:1984-09-01 00:00:00