Accessory factor-bZIP-DNA interactions.

abstract：:A wide assortment of caged compounds, which are species whose biological activity can be unleashed with light, have been synthesized and used to investigate a variety of biological phenomena. In contrast, the construction of caged proteins and their application to biological systems has lagged far behind. Recent advan...

journal_title：Current opinion in chemical biology

authors： Curley K,Lawrence DS

更新日期：1999-02-01 00:00:00

abstract：:New insights have been gained into the formation of the di-iron tyrosyl radical cofactor, which is essential for nucleotide reduction in the class I ribonucleotide reductases. Recent advances include Insight into the function of the tyrosyl radical in initiation of nucleotide reduction. ...

journal_title：Current opinion in chemical biology

authors： Stubbe J

更新日期：2003-04-01 00:00:00

abstract：:The eukaryotic ribonucleoprotein reverse transcriptase (RT) telomerase uses a template within its integral RNA subunit to extend chromosome ends by synthesis of single-stranded telomeric repeats. Telomerase is adapted to its unique cellular role by an ability to release product DNA in single-stranded form, regeneratin...

journal_title：Current opinion in chemical biology

authors： Collins K

更新日期：2011-10-01 00:00:00

abstract：:Lipids are a vital class of molecules that play important and varied roles in biological processes, however, fully understanding these roles is extremely difficult due to the immense number and diversity of possible lipid species. While recent advances in chromatography and high resolution mass spectrometry have great...

journal_title：Current opinion in chemical biology

authors： Zheng X,Smith RD,Baker ES

更新日期：2018-02-01 00:00:00

abstract：:The metal-free hydrogenase from methanogenic archaea (Hmd) is a unique enzyme: it catalyzes the reaction of its substrate, methenyl-tetrahydromethanopterin, with molecular hydrogen without the aid of a transition metal. In other words, Hmd is currently the only example of a purely organic hydrogenation catalyst. Recen...

journal_title：Current opinion in chemical biology

authors： Berkessel A

更新日期：2001-10-01 00:00:00

abstract：:Modular peptide synthetases, which act as the protein templates for the synthesis of a large number of peptide antibiotics and siderophores, hold great potential for the development of novel compounds. Recently, significant progress has been made towards understanding their molecular architecture and substrate specifi...

journal_title：Current opinion in chemical biology

authors： Mootz HD,Marahiel MA

更新日期：1997-12-01 00:00:00

abstract：:Inhibition of the steroid 5alpha-reductases shows promise in the treatment of a number of androgen-dependent disorders, such as benign prostatic hyperplasia, male pattern baldness, and acne. The design of potent and isozyme-selective inhibitors has provided biologists and clinicians with important tools for elucidatin...

journal_title：Current opinion in chemical biology

authors： Harris GS,Kozarich JW

更新日期：1997-08-01 00:00:00

abstract：:Medicinal chemistry principles are being increasingly applied to the design of smaller, high purity, information-rich libraries. Recent computational advances in statistical methodology, the design of libraries to reduce ADMET problems, targeting protein families and revisiting natural products as sources of inspirati...

journal_title：Current opinion in chemical biology

authors： Rose S,Stevens A

更新日期：2003-06-01 00:00:00

abstract：:Aside from the demonstration that individual molecular traits of enzymes can be evolutionarily optimised, the discovery that several traits can be simultaneously optimised is a major advance. The first observations of the effects of evolutionary optimisation at the structural level, through X-ray crystallography, rein...

journal_title：Current opinion in chemical biology

authors： Sutherland JD

更新日期：2000-06-01 00:00:00

abstract：:The cytochrome bc(1) complex is a key mitochondrial enzyme that catalyses transfer of electrons maintaining the membrane potential of mitochondria. Currently, atovaquone is the only drug in clinical use targeting the Plasmodium falciparum bc(1) complex. The rapid emergence of resistance to atovaquone resulted in a cos...

journal_title：Current opinion in chemical biology

authors： Barton V,Fisher N,Biagini GA,Ward SA,O'Neill PM

更新日期：2010-08-01 00:00:00

abstract：:The active site of cross-linked enzyme crystals can be further modified by chemical means, yielding a new type of chemically engineered enzyme. In the past year, the characteristics of cross-linked protein crystals as microporous materials have been studied and used as stationary phases in chromatographic separations....

journal_title：Current opinion in chemical biology

authors： Häring D,Schreier P

更新日期：1999-02-01 00:00:00

abstract：:Natural evolution has produced a great diversity of proteins that can be harnessed for numerous applications in biotechnology and pharmaceutical science. Commonly, specific applications require proteins to be tailored by protein engineering. Directed evolution is a type of protein engineering that yields proteins with...

journal_title：Current opinion in chemical biology

authors： Lane MD,Seelig B

更新日期：2014-10-01 00:00:00

abstract：:Although new detection screening methods must still be developed, the actual main limitation in combinatorial chemistry seems to be the diversity of ligands that can be generated in terms of real structural and chemical diversity. Thus, there is a strong interest for the development of different strategies for the par...

journal_title：Current opinion in chemical biology

authors： Lavastre O,Bonnette F,Gallard L

更新日期：2004-06-01 00:00:00

abstract：:Oligosaccharyl transferase is part of the macromolecular machinery that processes nascent proteins in the endoplasmic reticulum. The enzyme is highly conserved, catalyzes the initial step in the biosynthesis of N-linked glycoproteins and acts as a 'gatekeeper' for the secretory pathway. As more proteins associated wit...

journal_title：Current opinion in chemical biology

authors： Dempski RE Jr,Imperiali B

更新日期：2002-12-01 00:00:00

abstract：:The micro-environment in which stem cells reside regulates their fate, and synthetic materials have recently been designed to emulate these regulatory processes for various medical applications. Ligands inspired by the natural extracellular matrix, cell-cell contacts, and growth factors have been incorporated into syn...

journal_title：Current opinion in chemical biology

authors： Saha K,Pollock JF,Schaffer DV,Healy KE

更新日期：2007-08-01 00:00:00

abstract：:Transition-metal catalysis has unlocked new paradigms for the late-stage modification and cyclization of peptides by harnessing the innate reactivity of proteinogenic amino acids. The field is rapidly evolving, with recent advances encompassing three fundamental areas-heteroatom couplings, decarboxylative cross-coupli...

journal_title：Current opinion in chemical biology

authors： Malins LR

更新日期：2018-10-01 00:00:00

abstract：:Protein engineering over the past four years has made rhodopsin-based genetically encoded voltage indicators a leading candidate to achieve the task of reporting action potentials from a population of genetically targeted neurons in vivo. Rational design and large-scale screening efforts have steadily improved the dyn...

journal_title：Current opinion in chemical biology

authors： Gong Y

更新日期：2015-08-01 00:00:00

abstract：:Recent developments in the prediction of toxicity from chemical structure have been reviewed. Attention has been drawn to some of the problems that can be encountered in the area of predictive toxicology, including the need for a multi-disciplinary approach and the need to address mechanisms of action. Progress has be...

journal_title：Current opinion in chemical biology

authors： Barratt MD,Rodford RA

更新日期：2001-08-01 00:00:00

abstract：:Phosphorus cycling in the biosphere has traditionally been thought to involve almost exclusively transformations of the element in its pentavalent oxidation state. Recent evidence, however, suggests that a significant fraction of environmental phosphorus may exist in a more reduced form. Most abundant of these reduced...

journal_title：Current opinion in chemical biology

authors： Chin JP,McGrath JW,Quinn JP

更新日期：2016-04-01 00:00:00

abstract：:Initiating and terminating free-radical reactionis via hydrogen atom transfer (HAT) is an attractive means of avoiding substrate prefunctionalization. Small molecule catalysts and reagents, however, struggle to execute this fundamental step with useful levels of diastereoselectivity and enantioselectivity. In contrast...

journal_title：Current opinion in chemical biology

authors： Nakano Y,Biegasiewicz KF,Hyster TK

更新日期：2019-04-01 00:00:00

abstract：:There is currently intense interest in unravelling the modus operandi of type I modular polyketide synthases in order to lay the ground work for their use in the combinatorial biosynthesis of new bioactive molecules. Much of our knowledge is derived from studies on 6-deoxyerythronolide B (DEBS), the enzyme assembling ...

journal_title：Current opinion in chemical biology

authors： Floss HG,Yu TW

更新日期：1999-10-01 00:00:00

abstract：:Recently, the hypothesis that protein motions are involved in enzymatic turnover has gained significant attention. We review cases where there is evidence that protein motions are rate-limiting in the overall catalytic cycle and examine experimental and theoretical evidence for how such motions enhance the probability...

journal_title：Current opinion in chemical biology

authors： Nashine VC,Hammes-Schiffer S,Benkovic SJ

更新日期：2010-10-01 00:00:00

abstract：:Oligonucleotide in vitro selection provides surprisingly specific aptamers to protein targets. Synthetic chemistries for modifying nucleotides have been developed to enhance aptamer binding affinity. The diversity of nucleosides amenable to either triphosphate synthesis or phosphoramidite activation is now sufficientl...

journal_title：Current opinion in chemical biology

authors： Eaton BE

更新日期：1997-06-01 00:00:00

abstract：:Synthetic biologists typically construct new pathways within existing cells. While useful, this approach in many ways ignores the undefined but necessary components of life. A growing number of laboratories have begun to try to remove some of the mysteries of cellular life by building life-like systems from non-living...

journal_title：Current opinion in chemical biology

authors： Forlin M,Lentini R,Mansy SS

更新日期：2012-12-01 00:00:00

abstract：:Nitrification is the aerobic process of the nitrogen cycle that converts ammonia to nitrate and is facilitated by ammonia-oxidizing and nitrite-oxidizing microorganisms. Ammonia-oxidizers are unique chemolithotrophs that evolved specialized networks of electron carriers to generate proton motive force using ammonia as...

journal_title：Current opinion in chemical biology

authors： Stein LY

更新日期：2019-04-01 00:00:00

abstract：:Over the past two decades, peptides have expanded the toolbox of additives for the preparation of noble metal nanoparticles. Their functional and structural modularity and accompanying molecular recognition and self-assembly properties offer unique opportunities for the controlled formation of NPs. Within this review,...

journal_title：Current opinion in chemical biology

authors： Corra S,Shoshan MS,Wennemers H

更新日期：2017-10-01 00:00:00

abstract：:Molecular oxygen plays an indispensable role as a terminal electron acceptor in the electron transport chain in mitochondria. Acute or chronic oxygen deprivation (hypoxia) in organisms results in various diseases, and the elucidation of the pathogenic mechanism of hypoxia-related diseases and various cellular response...

journal_title：Current opinion in chemical biology

authors： Tobita S,Yoshihara T

更新日期：2016-08-01 00:00:00

abstract：:Oxidative cysteine modifications have emerged as a central mechanism for dynamic post-translational regulation of all major protein classes and correlate with many disease states. Elucidating the precise roles of cysteine oxidation in physiology and pathology presents a major challenge. This article reviews the curren...

journal_title：Current opinion in chemical biology

authors： Leonard SE,Carroll KS

更新日期：2011-02-01 00:00:00

abstract：:Chromatin organization spans a wide range of structural complexity. Substructures at the 10-200nm scale are poorly characterized, especially in living cells, due to the limitations of electron microscopy and standard optical microscopy. Recently developed super-resolution fluorescence microscopy methods represent an e...

journal_title：Current opinion in chemical biology

authors： Flors C,Earnshaw WC

更新日期：2011-12-01 00:00:00

abstract：:Metabolomics and lipidomics aim to comprehensively measure the dynamic changes of all metabolites and lipids that are present in biological systems. The use of ion mobility-mass spectrometry (IM-MS) for metabolomics and lipidomics has facilitated the separation and the identification of metabolites and lipids in compl...

journal_title：Current opinion in chemical biology

authors： Zhou Z,Tu J,Zhu ZJ

更新日期：2018-02-01 00:00:00