Abstract:
PURPOSE:Active HCO3- transport through the corneal endothelial cell layer causes a dehydration of the corneal stroma and is thought to be driven by Na/K- and HCO3(-)-dependent ATPase as well as an electro-genic Na/HCO3- cotransport. Transmembrane bicarbonate transport has also been associated with the recently characterised membrane-anchored isoform of carbonic anhydrase (CA IV) in various tissues. We investigated the localisation of CA IV in human fresh and cultured epi- and endothelium at the light- (LM) and electron-microscopic (EM) level. METHODS:Postmortem corneas were obtained within 12 hours of death, stored in formaldehyde and sectioned in paraffin. LM immunohisto-chemistry was performed using the purified gamma-globulin fraction of a polyclonal chicken antibody against CA IV isolated from human kidneys. Epi- and endothelial cell cultures were grown in uncoated flasks under standard conditions and processed both for LM and EM immunohistochemistry using the same antibody. RESULTS:Lightmicroscopy of fresh tissue showed membrane staining for CA IV in the whole circumference of the endothelium. Little staining was also observed in some cells of the basal cell layer of the epithelium. Immunohistochemical staining at the EM level was confined to the cell surface of confluent cultures of both epi- and endothelial cells. CONCLUSION:The localisation of CA IV to the cell surface of fresh and cultured corneal endothelium suggests the presence of a membrane-bound ion exchange mechanism which may be important for HCO3- transport and corneal hydration. Compromising this mechanism by treatment with local carbonicanhydrase inhibitors may be of clinical importance in selected endothelial disease.
journal_name
Klin Monbl Augenheilkdjournal_title
Klinische Monatsblatter fur Augenheilkundeauthors
Wolfensberger TJ,Mahieu I,Carter ND,Hollande E,Böhnke Mdoi
10.1055/s-2008-1034787subject
Has Abstractpub_date
1999-05-01 00:00:00pages
263-5issue
5eissn
0023-2165issn
1439-3999journal_volume
214pub_type
杂志文章abstract::Instead of the operating doublemicroscope described by Harms the Zeiss slit-lamp was suspended on the microscope stand by means of two special couplings in form of two stairs, so that the biomicroscopy in the optic section can be performed also when operating, especially in surgery of the retina when using contact gla...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:
更新日期:1977-05-01 00:00:00
abstract::Novel microscope-integrated OCT now allows targeted intraoperative high-resolution real-time imaging. In comparison to conventional surgical microscopes, intraoperative real-time OCT visualisation of retinal layers and vitreous body provides valuable information for surgical decision-making. In addition, simultaneous ...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/a-1193-9045
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND:In cataract surgery high quality is demanded. That is why high precision in calculation of IOL power is necessary for predicting postoperative refraction. MATERIALS AND METHODS:In a series of 103 patients the predicted refraction--calculated by the SRK II formula for only one type of IOL (A-constant 118.9)-...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1035289
更新日期:1996-08-01 00:00:00
abstract::Report on a catamnestic study which attempted to follow up patients who had undergone successful surgery for retinal detachment. A striking finding was that, even today, the vast majority of doctors discourage strenuous physical activity and sport. One of the reasons for this seems to be the discussion concerning the ...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1057344
更新日期:1981-12-01 00:00:00
abstract::Diagnosis and treatment of disturbances of binocular vision are made possible by a new orthoptic training device, which is a combination of Bagolini glasses, double prisms and a fixation target which can be shifted sagitally. It combines fusion, accommodation and convergence. ...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1057445
更新日期:1980-02-01 00:00:00
abstract:INTRODUCTION:Sarcoidosis can manifest itself in a variety of affection patterns in the eye. Early diagnosis and treatment are of crucial importance. Therefore the following three cases present occular symptoms associated with sarcoidosis. METHODS:Three case reports are described. RESULTS:We report three cases with pa...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-0032-1328160
更新日期:2013-05-01 00:00:00
abstract::Over a period of 4 months the authors observed 240 outpatients with 302 "red eyes." Oculogenital strains of Chlamydia trachomatis were diagnosed in 12.5% of these cases. Bacterial monoinfections were found in 13.9%, coinfections in 63.9%. The remaining 9.7% included Candida sp. (4%) and Herpes I (1.4%) infections, whi...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1054593
更新日期:1984-09-01 00:00:00
abstract::Dry-eye is a multifactorial disease consisting of different pathomechanisms including instability of the tearfilm, increase of tearfilm osmolarity and most importantly inflammation of the ocular surface. In this context numerous signalling pathways and related immune mechanisms were identified all pointing towards aut...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章,评审
doi:10.1055/s-0031-1299519
更新日期:2012-05-01 00:00:00
abstract::Description of various affections of the iris which may be treated with argon-laser. The problem of the laser iridectomy in the treatment of glaucoma is illustrated by a histologic study of the evolution of the lesions up to four months after coagulation of rabbit's iris. ...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1055016
更新日期:1982-01-01 00:00:00
abstract::Out of 1300 eyes enucleated between 1966-1974, 300 subsequently revealed histologically-proved malignant melanomas of the uvea. In 264 cases the clinical diagnosis was correctly made. In 36 cases the i.o. malignant melanomas were clinically unsuspected. The incorrect clinical diagnoses included: secondary glaucoma (30...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:
更新日期:1976-03-01 00:00:00
abstract:BACKGROUND:The aim of this study is to report the feasibility of one to two weeks delayed reconstruction after eyelid tumor excision. PATIENTS AND METHODS:A retrospective case-series study was designed. Fourteen consecutive outpatients referred to a tertiary referral eye center for eyelid tumor management were enrolle...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-0031-1299208
更新日期:2012-04-01 00:00:00
abstract:AIM:The aim of this study was to evaluate the functional effect of bilateral implantation of apodised diffractive versus progressive multizonal refractive multifocal intraocular lenses compared to standard monofocal intraocular lenses. PATIENTS AND METHOD:229 patients underwent cataract extraction; 66 Array SA40N (AMO...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-0032-1328642
更新日期:2013-08-01 00:00:00
abstract:BACKGROUND:We present the description of a successful outcome of intravitreal triamcinolone injection (IVTA) in a patient with cystoid macular oedema (CME) and severe retinal vasculitis suggestive of ocular Behçet's disease. HISTORY AND SIGNS:A healthy 32 year-old Caucasian man presented initially with right retinal v...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1027351
更新日期:2008-05-01 00:00:00
abstract:BACKGROUND:MALT Lymphoma of the conjunctiva, eyelid and orbit represents a primary manifestation of the disease in most of the cases. CASE REPORT:A 58-year-old male presented with a tumor in the left inferior eyelid. A full-thickness incisional biopsy was performed. Histopathologically, a lymphoid infiltrate involved ...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2000-10398
更新日期:2000-08-01 00:00:00
abstract::During a 4-year period 22 eyes with rhegmatogenous retinal detachment in children and young adults up to 20 years of age were operated on. This group comprised 6.3 percent of all rhegmatogenous retinal detachments operated on during the same period. In 9 eyes (40.9%) the detachment was due to a direct ocular trauma. I...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1054561
更新日期:1984-07-01 00:00:00
abstract::Brachytherapy with 106Ru/106Rh necessitates protracted irradiation because the applicators have to be sutured to the sclera. Protracted radiotherapy causes more intensive damage in tumor cells with increased metabolism and a higher mitosis rate than in normal tissue. Beta irradiation of rabbit eyes with 0.258-0.231 Gy...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1050770
更新日期:1986-08-01 00:00:00
abstract::Für die (Früh-)Diagnose einer Glaukomerkrankung ist die Beurteilung der Papille von entscheidender Bedeutung. Das Spektrum von High-Tech-Geräten zur Papillenbeurteilung hat sich in den letzten Jahren erweitert und die Anwendung ist in der klinischen Praxis angekommen 1. Voraussetzung für die sinnvolle Anwendung und In...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-0033-1358179
更新日期:2015-09-01 00:00:00
abstract::Scanning laser tomography (HRT, HRT II), scanning laser polarimetry (GDx nerve fibre analyser, GDx VCC), retinal thickness analyser (RTA) and optical coherence tomography (OCT, stratus OCT) provide objective measurements of the optic disc and the retinal nerve fibre layer suitable for the follow-up of glaucoma patient...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章,评审
doi:10.1055/s-2005-859004
更新日期:2006-06-01 00:00:00
abstract::In this clinicopathological case report on a 27-year-old man suffering from mucopolysaccharidosis (MPS) type VI-A (Maroteaux-Lamy), histologic findings in both eyes, and histologic and ultrastructural findings in a keratoplasty button are presented for the first time in the German literature. Type VI-A is a very rare ...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1046370
更新日期:1989-04-01 00:00:00
abstract:BACKGROUND:Can the capsulotomy with small radial incisions compared to the continuous curvilinear capsulorhexis with perfect IOL centration still be recommended? This problem should be clarified under standard conditions. METHOD:On 100 patients both procedures were applied alternating with corneoscleral incision, phac...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1035520
更新日期:1994-04-01 00:00:00
abstract::Topical administration of Isoproterenol reduces the intraocular pressure in glaucomatous eyes, but produces systemic side-effects (tachycardia arterial hypotension) that make its clinical use unadvisable. Such side-effects are significant when the concentration of Isoproterenol is 4 and 5%. Pre-treatment of the contra...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:
更新日期:1977-04-01 00:00:00
abstract:BACKGROUND:The postoperative clinical course after penetrating keratoplasty and trephination in free form using a guided excimer laser beam has been published before. Here the findings of light-microscopy comparing corneal wound healing after experimental penetrating keratoplasty after laser trephination and after conv...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 评论,杂志文章
doi:10.1055/s-2006-927097
更新日期:2006-12-01 00:00:00
abstract:BACKGROUND:Gene therapy has gained increasing attention and a number of ongoing clinical trials have been initiated. This article provides current perspectives and limitations on gene therapy in ophthalmology. Since a number of comprehensive studies on gene therapy for retinal diseases already exist, we focus attention...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章,评审
doi:10.1055/s-2001-13073
更新日期:2001-03-01 00:00:00
abstract::Fifty patients with open-angle glaucoma were treated twice daily for one year with topical 0.5% levobunolol, 1% levobunolol, or 0.5% timolol. Both concentrations of levobunolol were as effective as timolol in reducing intraocular pressure over the one-year period. At the concentrations tested, levobunolol and timolol ...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1055/s-2008-1054395
更新日期:1985-12-01 00:00:00
abstract:BACKGROUND:A retinal image performance distorted by an asymmetric or irregular corneal surface cannot be compensated for with spherocylindric glasses completely. The best-corrected visual acuity is markedly decreased and contact lens fitting often impossible. The purpose of this study was to calculate the differential ...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1034963
更新日期:1998-09-01 00:00:00
abstract:BACKGROUND:Cat scratch disease (CSD) is a common infectious disease, however its association with a disciforme keratitis is to our knowledge a previously unreported ocular complication. PATIENT:A 65-year old female was treated unsuccessfully with topical antibiotics, acyclovir and corticosteroids for several weeks bec...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2000-10372
更新日期:2000-11-01 00:00:00
abstract:BACKGROUND:A meticulous fundoscopy is an essential examination before administering an orthoptic occlusion therapy (patching), even in cases with a "clear indication" for patching. In this case of an 8-year-old hyperopic boy the subsequent fundoscopy revealed a bilateral maculopathy that explained the stagnation of the...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1027270
更新日期:2008-05-01 00:00:00
abstract::On the occasion of the 150th aniversary of Albrecht von Graefe's birthday his short but successful activity for ophthalmology is brought back to mind. Within the scape of his life-history the attempt is made to portray a man in his time and to waken his importance as ophthalmologist a significant still in our days. ...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 传,历史文章,杂志文章
doi:
更新日期:1978-05-01 00:00:00
abstract::In two groups of patients with two different posterior chamber intraocular lenses, very different correlations were found between postoperative and calculated anterior chamber depth, and between postoperative anterior chamber depth and axial length. Since it has long been known that there are positive correlations bet...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1046396
更新日期:1989-06-01 00:00:00
abstract::The suitability of ultrasonography, computer tomography and Octopus perimetry is illustrated with reference to an investigation carried out in 60 patients. First, the characteristic symptoms are described, depending on the localization of the lesion in the visual pathway. Then the advantages and disadvantages of the v...
journal_title:Klinische Monatsblatter fur Augenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1055172
更新日期:1982-08-01 00:00:00