Abstract:
OBJECTIVE:Our purpose was to determine whether cultured human decidual cells produce chemokines in response to different strains of group B streptococci and purified bacterial cell wall components. STUDY DESIGN:Human decidual cells were cultured from term placentas by standard techniques. Different strains of group B streptococci were isolated from neonates with early-onset group B streptococci sepsis. Confluent cell monolayers were incubated with these different strains of group B streptococci and various concentrations of purified bacterial cell wall components (including lipoteichoic acid, sialic acid, lipopolysaccharide, and lipid A) for 16 hours at 37 degrees C. Culture supernatants were collected and assayed for macrophage inflammatory protein-1 alpha and interleukin-8. Statistical analysis was by analysis of variance. RESULTS:We found that cultured human decidual cells produced significant amounts of the two chemokines macrophage inflammatory protein-1 alpha and interleukin-8 in a strain-specific fashion to the various different strains of group B streptococci tested, from 215% to 421% over baseline production (p < 0.05 by analysis of variance). Also, we found that incubation of decidual cells with various concentrations of lipoteichoic acid, sialic acid, lipopolysaccharide, and lipid A resulted in significant concentration-dependent increases in decidual cell macrophage inflammatory protein-1 alpha and interleukin-8 production (p < 0.05.) CONCLUSIONS:Decidual cells produced significant amounts of the chemokines macrophage inflammatory protein-1 alpha and interleukin-8 in response to intact group B streptococci in a strain-specific fashion and in response to various concentrations of different bacterial cell wall components. Because chemokines are important mediators signaling migration of different immune effector cells into areas of inflammation, we suggest that decidual cell chemokine production in response to bacteria and bacterial cell wall components may be a key early event in the pathogenesis of infection-associated preterm labor.
journal_name
Am J Obstet Gynecoljournal_title
American journal of obstetrics and gynecologyauthors
Dudley DJ,Edwin SS,Van Wagoner J,Augustine NH,Hill HR,Mitchell MDdoi
10.1016/s0002-9378(97)70162-5subject
Has Abstractpub_date
1997-09-01 00:00:00pages
666-72issue
3eissn
0002-9378issn
1097-6868pii
S0002-9378(97)70162-5journal_volume
177pub_type
杂志文章abstract::A case discussing the medical management of a 30-year-old gravid patient with recurrent pericarditis and pericardial constriction secondary to juvenile rheumatoid arthritis is presented. ...
journal_title:American journal of obstetrics and gynecology
pub_type: 杂志文章
doi:10.1016/0002-9378(86)90418-7
更新日期:1986-01-01 00:00:00
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更新日期:1990-07-01 00:00:00
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journal_title:American journal of obstetrics and gynecology
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更新日期:1985-05-15 00:00:00
abstract::Serum progesterone was assayed by the competitive protein-binding technique in 20 cases of gestational choriocarcinoma and one case of nongestational choriocarcinoma. Serum human chorionic gonadotropin (HCG) was simultaneously measured from the same blood samples by the hemagglutination-inhibition technique. Serum pro...
journal_title:American journal of obstetrics and gynecology
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journal_title:American journal of obstetrics and gynecology
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更新日期:1992-07-01 00:00:00
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journal_title:American journal of obstetrics and gynecology
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1999-07-01 00:00:00
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更新日期:1982-05-01 00:00:00
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journal_title:American journal of obstetrics and gynecology
pub_type: 临床试验,杂志文章,评审
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更新日期:1986-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0002-9378(11)91417-3
更新日期:1992-08-01 00:00:00
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journal_title:American journal of obstetrics and gynecology
pub_type: 杂志文章
doi:10.1016/j.ajog.2013.04.020
更新日期:2013-08-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:1992-02-01 00:00:00
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journal_title:American journal of obstetrics and gynecology
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1991-12-01 00:00:00
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1999-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-10-01 00:00:00
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更新日期:2014-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0002-9378(74)90117-3
更新日期:1974-07-01 00:00:00
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journal_title:American journal of obstetrics and gynecology
pub_type: 杂志文章
doi:10.1016/s0002-9378(96)70095-9
更新日期:1996-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-08-15 00:00:00
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pub_type: 杂志文章
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更新日期:1995-10-01 00:00:00
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journal_title:American journal of obstetrics and gynecology
pub_type: 杂志文章
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更新日期:1996-05-01 00:00:00
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更新日期:1988-06-01 00:00:00
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journal_title:American journal of obstetrics and gynecology
pub_type: 杂志文章
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更新日期:1981-10-15 00:00:00
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journal_title:American journal of obstetrics and gynecology
pub_type: 杂志文章
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更新日期:2004-08-01 00:00:00
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journal_title:American journal of obstetrics and gynecology
pub_type: 杂志文章
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更新日期:1994-08-01 00:00:00
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journal_title:American journal of obstetrics and gynecology
pub_type: 杂志文章
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更新日期:2011-07-01 00:00:00