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Osmotic lysis of tumor spill in ovarian cancer: a murine model.

Abstract：

OBJECTIVE:Our purpose was to determine, in the murine model, whether human ovarian cancer cells injected intraperitoneally are subject to osmotic lysis by peritoneal lavage with sterile water, thereby decreasing the establishment of peritoneal implants. STUDY DESIGN:Preliminary experiments on six nude mice determined that the injection of 20 million cells of the SKOV-3 cell line reliably leads to the establishment of intraperitoneal tumor xenografts in the mice within 60 days. Four other nude mice functioned as sham controls undergoing peritoneal lavage with 3 to 4 ml of saline solution or sterile water to determine any adverse effects from the lavage alone. Subsequently, 36 nude (nu/nu) mice were injected intraperitoneally with 1 ml of the SKOV-3 cell line at a concentration of 20 million cells per milliliter. Alternate mice then underwent intraperitoneal lavage with either 3 to 4 ml of normal saline solution (control group) or sterile water (study group). The mice were followed up until tumor growth caused a moribund status or until 60 days after injection and then were killed. At necropsy the number and size of tumor nodules were recorded, and each mouse was assigned a composite tumor score. Statistical comparison used the X2 or Fisher's exact test for discrete variables. Time to failure analysis used the Kaplan-Meier method. RESULTS:Tumor growth occurred in 35 of 36 (97%) of the mice during the study period. In the first 30 days 89% of the saline solution group grew clinically visible tumor compared with 55% of the water group (p = 0.03). Ascites developed more frequently in the water group than in the saline solution group. The median tumor scores at death were significantly higher for the water group versus the saline solution group. Survival time, as determined by the time from injection until moribund status, was worse for the water group (p = 0.002). CONCLUSIONS:Intraperitoneal lavage with sterile water did not offer protection against the establishment of xenografts after the intraperitoneal injection of human ovarian cancer cells in the nude mouse model.

journal_name

Am J Obstet Gynecol

journal_title

American journal of obstetrics and gynecology

authors

Morris PC,Scholten V

doi

10.1016/s0002-9378(96)70095-9

subject

Has Abstract

pub_date

1996-12-01 00:00:00

pages

1489-92

issue

eissn

0002-9378

issn

1097-6868

pii

S0002-9378(96)70095-9

journal_volume

175

pub_type

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Osmotic lysis of tumor spill in ovarian cancer: a murine model.