Diagnosis of primary human herpesvirus 6 and 7 infections in febrile infants by polymerase chain reaction.

Abstract:

:Primary human herpesvirus 6 (HHV-6) and 7 (HHV-7) infections were identified in febrile children by qualitative and quantitative polymerase chain reaction (PCR) assays. Diagnosis was based on the differential detection of viral DNA in peripheral blood mononuclear cells (PBMC), but not in saliva. Six of 41 febrile infants, but none of seven non-febrile controls, were identified with primary infections (three HHV-6, three HHV-7). These children had significantly higher viral loads in PBMC (HHV-6, median 24213 genomes/10(6) PBMC; HHV-7, median 6,040,000 genomes/10(6) PBMC) than DNA-aemic, saliva PCR positive children (HHV-6, median 1606 genomes/10(6) PBMC, p < 0.01; HHV-7, median 7089 genomes/ 10(6) PBMC, p < 0.05). Viral DNA was detected in serum by PCR in only 50% of primary infections. All three children with primary HHV-7 infection had febrile convulsions. Thus PCR, including quantitative assays, may identify primary HHV-6 and HHV-7 infections when an appropriate combination of clinical specimens is used.

journal_name

Arch Dis Child

authors

Clark DA,Kidd IM,Collingham KE,Tarlow M,Ayeni T,Riordan A,Griffiths PD,Emery VC,Pillay D

doi

10.1136/adc.77.1.42

subject

Has Abstract

pub_date

1997-07-01 00:00:00

pages

42-5

issue

1

eissn

0003-9888

issn

1468-2044

journal_volume

77

pub_type

杂志文章
  • How has research changed my practice in the last 5 years?

    abstract::You can't teach old dogs new tricks. Teaching middle-aged dogs new tricks is hard enough. They tend to stick to particular styles of attacking the postman that over the years have proved pretty reliable, often choosing to ignore new findings, or simply failing to keep up with progress in current discussions on, say, t...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.2006.094961

    authors: Cameron D

    更新日期:2007-11-01 00:00:00

  • Tartrazine in atopic eczema.

    abstract::Multiple double blind placebo controlled challenges with tartrazine 50 mg (three challenges) and glucose placebo (three challenges) were performed in 12 children with atopic eczema aged 1 to 6 years. The children were selected on the basis of severity (regular clinic attenders) and a parental history that tartrazine p...

    journal_title:Archives of disease in childhood

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1136/adc.67.6.709

    authors: Devlin J,David TJ

    更新日期:1992-06-01 00:00:00

  • Monitoring of cerebral function after severe asphyxia in infancy.

    abstract::Thirty nine infants with severe asphyxia (28 affected perinatally and 11 later) were studied by electrophysiological cerebral function monitoring (CFM) for periods varying from a half to 49 days. Nineteen infants died while still in intensive care and two died later from sequelae. Eighteen survived and were followed u...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.58.12.997

    authors: Bjerre I,Hellström-Westas L,Rosén I,Svenningsen N

    更新日期:1983-12-01 00:00:00

  • Paediatric emergency department anaphylaxis: different patterns from adults.

    abstract:BACKGROUND AND AIMS:Data on acute paediatric anaphylaxis presentations to the emergency department (ED) are limited. All allergic presentations to one Australian paediatric ED were studied to determine epidemiological, clinical, and outcome data. METHODS:Retrospective, case based study of patients under 16 years atten...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.2004.069914

    authors: Braganza SC,Acworth JP,Mckinnon DR,Peake JE,Brown AF

    更新日期:2006-02-01 00:00:00

  • Change in blood pressure after treatment of patent ductus arteriosus with indomethacin.

    abstract::The effect of indomethacin treatment of patent ductus arteriosus (PDA) on blood pressure was studied in 24 preterm infants. PDA was diagnosed clinically and confirmed by echocardiography; the effect of treatment was monitored echocardiographically. Hourly intra-arterial recordings of systolic, diastolic, and mean bloo...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.68.5_spec_no.584

    authors: Evans N,Iyer P

    更新日期:1993-05-01 00:00:00

  • Use of peripheral vessels for exchange transfusion.

    abstract::During a five and a half year period, exchange transfusions were performed through the peripheral vessels in 201 of the 214 infants (94%) who required either double volume or partial plasma exchange transfusions. Peripheral vessel exchange transfusion is simple, practicable, and safe with few complications. Technical ...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.65.7_spec_no.676

    authors: Fok TF,So LY,Leung KW,Wong W,Feng CS,Tsang SS

    更新日期:1990-07-01 00:00:00

  • Impact of oral corticosteroids on respiratory outcomes in acute preschool wheeze: a randomised clinical trial.

    abstract:OBJECTIVE:To determine if administration of oral prednisolone to preschool children with acute wheeze alters respiratory outcomes. DESIGN:Double-blind, randomised, placebo-controlled equivalence trial. SETTING:Three hospitals in New Zealand. PATIENTS:477 children aged 24-59 months with acute wheeze associated with r...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/archdischild-2020-318971

    authors: Wallace A,Sinclair O,Shepherd M,Neutze J,Trenholme A,Tan E,Brabyn C,Bonisch M,Grey N,Johnson DW,McNamara D,Thompson JMD,Asher I,Dalziel SR

    更新日期:2020-10-16 00:00:00

  • Primary infections of Epstein-Barr virus, cytomegalovirus, and human herpesvirus-6.

    abstract::A total of 121 infants entered a cohort serological study of primary infections with herpes-viruses. All of them had seven samples of blood available: the first sample was taken soon after birth, the other six were taken at 1, 2, 3, 6, 12, and 14 months of age. One sample of maternal blood was collected immediately af...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.68.3.408

    authors: Huang LM,Lee CY,Chang MH,Wang JD,Hsu CY

    更新日期:1993-03-01 00:00:00

  • Why breast feeding fails.

    abstract:BACKGROUND:Vanessa is a 34-year-old lawyer married to a police officer. Her first baby was born after a perfect pregnancy and labour until fetal distress necessitated an easy Ventouse extraction late in the second stage. Baby F had normal Apgar scores and weighed 2920 g. This paper records a conversation between Vaness...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.2006.101600

    authors: Harris V,Hall D

    更新日期:2007-01-01 00:00:00

  • Delayed adolescent growth in homozygous sickle cell disease.

    abstract::Analysis of the growth abnormalities in sickle cell disease has been limited by the lack of longitudinal observations in individuals, and by an inability to quantitate the observed patterns. To investigate the timing and pattern of the adolescent growth spurt, longitudinal observations of height from the Jamaican coho...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.71.5.404

    authors: Singhal A,Thomas P,Cook R,Wierenga K,Serjeant G

    更新日期:1994-11-01 00:00:00

  • The feasibility and acceptability of collecting oral fluid from healthy children for anti-HCV testing.

    abstract::This pilot study investigated the feasibility of surveying, anonymously, HCV infection among healthy children using an oral fluid specimen. Seventy seven per cent of children provided their assent, or where appropriate, consent to participate; 2.8% were anti-HCV positive. Oral fluid collection is acceptable to childre...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.2003.031310

    authors: Chatzipantazi P,Roy KM,Cameron SO,Goldberg D,Welbury R,Bagg J

    更新日期:2004-02-01 00:00:00

  • Henoch-Schönlein syndrome and selective IgA deficiency.

    abstract::A 9 year old girl presented with clinical manifestations of Henoch-Schönlein syndrome and macroscopic haematuria. Laboratory investigations showed selective IgA deficiency and renal biopsy showed mesangial proliferative glomerulonephritis with diffuse granular deposits of C3 on immunofluorescence. IgA deposits were ab...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.60.2.160

    authors: Martini A,Ravelli A,Notarangelo LD,Burgio VL,Plebani A

    更新日期:1985-02-01 00:00:00

  • Efficacy and safety of torasemide in children with heart failure.

    abstract:OBJECTIVE:To examine the efficacy and safety of torasemide in children with chronic heart failure (HF). METHODS:102 children with chronic HF who had received oral torasemide were analysed. Of these, 62 (de novo group) were newly diagnosed as having HF and were given torasemide as a diuretic. The remaining 40 (replacem...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.2007.129239

    authors: Senzaki H,Kamiyama M,Masutani S,Ishido H,Taketazu M,Kobayashi T,Katogi T,Kyo S

    更新日期:2008-09-01 00:00:00

  • Metabolic and hormonal responses of low birthweight infants to intravenously infused calories not exceeding the maintenance energy expenditure.

    abstract::Eithe low birthweight infants were studied for their metabolic and hormonal responses to intravenous alimentation when the caloric input was varied. The study was made during three consecutive 12-hour periods, when solutions of glucose, glucose and amino-acids, and glucose and amino-acid and lipids were successively i...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.54.7.499

    authors: Rubecz I,Mestyán J,Varga P,Soltész G

    更新日期:1979-07-01 00:00:00

  • Motor disorders of the oesophagus in gastro-oesophageal reflux.

    abstract::Mechanisms of gastro-oesophageal reflux were studied by oesophageal manometry and pH monitoring in 33 children: nine controls, 15 with gastro-oesophageal reflux alone, and nine with reflux oesophagitis. A total of 122 episodes of reflux were analysed in detail: 82 (67%) were synchronous with swallowing and 40 (33%) as...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.63.11.1333

    authors: Mahony MJ,Migliavacca M,Spitz L,Milla PJ

    更新日期:1988-11-01 00:00:00

  • Management of severe acute malnutrition in low-income and middle-income countries.

    abstract::Kwashiorkor and marasmus, collectively termed severe acute malnutrition (SAM), account for at least 10% of all deaths among children under 5 years of age worldwide, virtually all of them in low-income and middle-income countries. A number of risk factors, including seasonal food insecurity, environmental enteropathy, ...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/archdischild-2014-306026

    authors: Trehan I,Manary MJ

    更新日期:2015-03-01 00:00:00

  • Serum pancreatic lipase as a screening test for cystic fibrosis.

    abstract::Pancreatic lipase catalyses the hydrolysis of emulsified triglycerides to form a transparent solution of monoglycerides and fatty acids. Levels of serum pancreatic lipase were measured in neonates known to have cystic fibrosis and compared with levels in control infants. During the first weeks of life infants with cys...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.57.7.553

    authors: Adriaenssens K,Van Riel L

    更新日期:1982-07-01 00:00:00

  • Inconsistencies in sweat testing in UK laboratories.

    abstract:BACKGROUND:Sweat testing procedures are perceived to vary widely. AIM:To evaluate variability in sweat collection, analysis, and interpretation. METHODS:Questionnaire responses from 30 self selected centres: 15 paediatric centres, and 15 district general hospitals. RESULTS:Centres carried out 30-400 tests per year (...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章,多中心研究

    doi:10.1136/adc.82.5.425

    authors: Kirk JM

    更新日期:2000-05-01 00:00:00

  • Bacteriological monitoring of unheated human milk.

    abstract::To assess the bacteriological quality of unpooled expressed breast milk, a pilot bottle sample of each donation was examined before the milk was given to the neonate. Provided the milk did not contain greater than 2500 organisms/ml or potential pathogens it was used unheated. Milk containing between 2500 and 5000 orga...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.54.10.760

    authors: Davidson DC,Poll RA,Roberts C

    更新日期:1979-10-01 00:00:00

  • Simple method for securing umbilical catheters.

    abstract::A method for securing umbilical catheters is described. It has been used successfully in over 350 babies. It is a simple and rapid method that allows for easy repositioning and, unlike some established techniques, has no potential for damage to the preterm baby's skin. ...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.63.7_spec_no.750

    authors: South M,Magnay A

    更新日期:1988-07-01 00:00:00

  • Respiratory health in a total very low birthweight cohort and their classroom controls.

    abstract:AIMS:To compare the respiratory health and function at 8 to 9 years of age of a total population based cohort of 300 very low birthweight (VLBW) children with that of two classroom controls (n = 590) matched for age and sex. STUDY DESIGN:Cohort study with controls. SETTING:Schools throughout Scotland. RESULTS:The VL...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.74.3.188

    authors: McLeod A,Ross P,Mitchell S,Tay D,Hunter L,Hall A,Paton J,Mutch L

    更新日期:1996-03-01 00:00:00

  • Biotinidase deficiency: a survey of 10 cases.

    abstract::Ten patients with biotinidase deficiency were studied. Clinical findings at presentation varied with dermatological signs (dermatitis and alopecia), neurological abnormalities (fits, hypotonia, and ataxia), and recurrent infections being the most common features, although none of these occurred in every case. Biochemi...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.63.10.1244

    authors: Wastell HJ,Bartlett K,Dale G,Shein A

    更新日期:1988-10-01 00:00:00

  • Human metapneumovirus infections in hospitalised infants in Spain.

    abstract:BACKGROUND:Human metapneumovirus (hMPV) causes lower respiratory tract infections, particularly in young children and the elderly. METHODS:A prospective study was conducted on the clinical characteristics of infants <2 years of age admitted to hospital for respiratory infection and the characteristics of hMPV infectio...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.2005.082388

    authors: García-García ML,Calvo C,Martín F,Pérez-Breña P,Acosta B,Casas I

    更新日期:2006-04-01 00:00:00

  • Insulin dependent diabetes in thalassaemia.

    abstract::Diabetes mellitus was observed in 29 of 448 patients with thalassaemia major attending seven Italian centres. Twelve patients, at onset of clinical diabetes, presented with an asymptomatic glycosuria, 13 with ketosis, and four with ketoacidosis. All were diagnosed after 1979, at a mean age of 17 years. Mean age at dia...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.63.1.58

    authors: De Sanctis V,Zurlo MG,Senesi E,Boffa C,Cavallo L,Di Gregorio F

    更新日期:1988-01-01 00:00:00

  • Dilemmas in undertaking research in paediatric intensive care.

    abstract::Providing evidence-based interventions for infants and children is important in paediatric intensive care, where decision making impacts most acutely on morbidity and mortality. However, despite the major progress of medicine in the 21st century, we still lack this evidence for majority of the decisions we make. In th...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章,评审

    doi:10.1136/archdischild-2013-304871

    authors: Kanthimathinathan HK,Scholefield BR

    更新日期:2014-11-01 00:00:00

  • Missed opportunities to vaccinate children admitted to a paediatric tertiary hospital.

    abstract:BACKGROUND:Inequalities in vaccine uptake exist. Studies suggest paediatric inpatients have lower rates of immunisation uptake than the general population. Various UK policies advocate opportunistic immunisation. AIM:To evaluate practice within a paediatric tertiary hospital in identifying and facilitating vaccination...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.2006.104778

    authors: Walton S,Elliman D,Bedford H

    更新日期:2007-07-01 00:00:00

  • Haem arginate in acute hereditary coproporphyria.

    abstract::An 11 year old boy presented with severe acute hereditary coproporphyria. Despite supportive measures his condition deteriorated after admission. Haem arginate, started two days after presentation, produced appreciable inhibition of porphyrin precursor overproduction and clinical improvement. ...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.66.6.730

    authors: Manning DJ,Gray TA

    更新日期:1991-06-01 00:00:00

  • Is cholecystectomy really an indication for concomitant splenectomy in mild hereditary spherocytosis?

    abstract:BACKGROUND/AIM:The British Committee for Standards in Haematology currently recommends concomitant splenectomy in children with mild hereditary spherocytosis (HS) undergoing cholecystectomy for symptomatic gallstones. However, splenectomy is associated with a risk of life-threatening infection, particularly in young ch...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/adc.2009.178228

    authors: Alizai NK,Richards EM,Stringer MD

    更新日期:2010-08-01 00:00:00

  • THE APPROPRIATENESS OF USING AVERAGE DISPLACEMENT VALUES FOR PAEDIATRIC INTRAVENOUS DRUG ADMINISTRATIONS.

    abstract:AIM:To determine the impact of displacement values on doses in paediatric patients when using parenteral. To assess the option of using average displacement values (DV) in the preparation of parenteral medicines. METHOD:Over 500 Medusa1 monographs were analysed and 42 medicines were identified with a displacement valu...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/archdischild-2016-311535.30

    authors: Lo A,Christiansen N,Bhatti H

    更新日期:2016-09-01 00:00:00

  • Post-renal transplant urolithiasis in children: an increasingly diagnosed complication: a retrospective cohort study.

    abstract:OBJECTIVE:Urolithiasis in renal transplant (RTx) recipients is a potential cause of allograft loss if obstruction is untreated. It is not clear if paediatric transplant recipients are following the global trend for increased prevalence of urolithiasis over time. DESIGN/SETTING/PATIENTS:A retrospective chart review was...

    journal_title:Archives of disease in childhood

    pub_type: 杂志文章

    doi:10.1136/archdischild-2019-317203

    authors: Ma S,Taher A,Zhu B,Durkan AM

    更新日期:2020-01-01 00:00:00